Article
Oncology
Chaoyan Wang, Menghan Weng, Shuli Xia, Min Zhang, Chaoyi Chen, Jinlong Tang, Dan Huang, Hongfei Yu, Wenjie Sun, Honghe Zhang, Maode Lai
Summary: This study investigated the distinct functions of different PD-L1 isoforms in colorectal cancer (CRC) and found that isoform b can be considered as a biomarker for responsiveness to anti-PD-1/PD-L1 immunotherapy, while isoform c has a prometastatic role in CRC.
Article
Genetics & Heredity
Qi Liu, Leiming Fang, Chengjun Wu
Summary: Alternative splicing of pre-mRNA is a key mechanism for increasing protein complexity, leading to diversity of transcriptomes and proteomes in a tissue-specific manner. Changes and errors in splicing regulation caused by splicing factors are closely related to various diseases. Understanding cellular factors that mediate alternative splicing regulation is a potential avenue for developing specific drugs targeting the dynamic RNA splicing process.
Review
Biochemistry & Molecular Biology
Andrea Corsi, Cristina Bombieri, Maria Teresa Valenti, Maria Grazia Romanelli
Summary: Tau microtubule-associated proteins play important roles in neurons and are associated with several neurodegenerative diseases. Recent studies have focused on understanding the regulation of Tau splicing to develop new treatment approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
A. Gallo, D. Dolfini, A. Bernardini, N. Gnesutta, R. Mantovani
Summary: NF-YA, the regulatory subunit of the trimeric CCAAT-binding transcription factor NF-Y, has multiple alternative spliced isoforms, and NF-YAx and NF-YAg are enriched in Aves and play a major role in early chicken embryo development. In addition, the absence of exon-5 in NF-YAg leads to a global reduction of beta-stranded elements, while removal of the disordered exon-3 sequence has limited effects on TAD architecture.
Article
Oncology
Carlos Enrique Balcazar Lopez, Juliane Albrecht, Voelundur Hafstao, Cornelia Borjesson Freitag, Johan Vallon-Christersson, Cristian Bellodi, Helena Persson
Summary: This study provides a comprehensive annotation of estrogen receptor alpha (ER) isoforms and investigates their functional differences. It also highlights the potential impact of antibody specificity on the interpretation of ER-status in breast cancer pathology.
Article
Biochemistry & Molecular Biology
Qingnan Liang, Nathaniel Wu, Smriti Zaneveld, Hehe Liu, Shangyi Fu, Keqing Wang, Renae Bertrand, Jun Wang, Yumei Li, Rui Chen
Summary: This study investigated the function and relationship of the two isoforms of Reep6 in rod photoreceptor cells. The findings suggested that the Reep6.1 isoform is essential for the survival of rod photoreceptor cells and has rod-specific functions which cannot be substituted by its canonical isoform. The strict regulation of splicing is crucial for the maintenance of photoreceptor cells.
HUMAN MOLECULAR GENETICS
(2021)
Article
Cell Biology
Yanjun Lu, Lu Tan, Jiazhao Xie, Liming Cheng, Xiong Wang
Summary: This study revealed microglia-specific alternative splicing events in Alzheimer's disease and identified novel pathological mechanisms related to AD.
Article
Plant Sciences
Shijia Zhang, Huili Liu, Li Yuan, Xiaojing Li, Lingbao Wang, Xiaodong Xu, Qiguang Xie
Summary: The study identified that CCA1 alpha and CCA1 beta protein variants respond to light and temperature cues, with higher temperatures promoting CCA1 beta protein production. The levels of CCA1 proteins peak in the morning and the detection of CCA1 beta is dependent on specific conditions at 22 degrees Celsius.
PLANT CELL REPORTS
(2021)
Article
Developmental Biology
Cansu Akkaya, Dila Atak, Altug Kamacioglu, Busra Aytul Akarlar, Gokhan Guner, Efil Bayam, Ali Cihan Taskin, Nurhan Ozlu, Gulayse Ince-Dunn
Summary: KIF2A is essential for dendritic arborization and radial migration of cortical neurons during brain development. Three Kif2a isoforms were studied, with only two supporting neuronal migration. A KIF2A mutation causing brain malformations led to changes in its interactome, including depletion of mitochondrial proteins.
Article
Neurosciences
Kerilyn Casey Omuro, Christina M. Gallo, Lauren Scrandis, Angela Ho, Uwe Beffert
Summary: Human APOER2 isoforms have differential cleavage events and synaptic properties.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Gastroenterology & Hepatology
Ming Zhang, Can Chen, Zequn Lu, Yimin Cai, Yanmin Li, Fuwei Zhang, Yizhuo Liu, Shuoni Chen, Heng Zhang, Shuhui Yang, Hui Gen, Yuan Jiang, Caibo Ning, Jinyu Huang, Wenzhuo Wang, Linyun Fan, Yi Zhang, Meng Jin, Jinxin Han, Zhen Xiong, Ming Cai, Jiuyang Liu, Chaoqun Huang, Xiaojun Yang, Bin Xu, Heng Li, Bin Li, Xu Zhu, Yongchang Wei, Ying Zhu, Jianbo Tian, Xiaoping Miao
Summary: This study provides a comprehensive functional analysis of splicing quantitative trait loci (sQTLs) in cancer and specifically in colorectal cancer (CRC). The researchers identified genetic variants that control messenger RNA splicing and examined their association with CRC risk. They also conducted biological experiments to investigate the potential mechanisms of these sQTLs and target genes. The study highlights the role of sQTLs in cancer susceptibility and suggests their potential as biomarkers and therapeutic targets.
Review
Biology
Showkat Ahmad Ganie, Anireddy S. N. Reddy
Summary: When facing environmental stresses, alternative splicing (AS) plays a crucial role in rice by rapidly regulating gene expression to help the plant adapt to various pressures. Studies have shown that AS is important for rice growth and development, especially under stress conditions. Understanding the impact of AS on rice and its pathogens can provide valuable insights for developing stress-resistant rice varieties in the future.
Article
Genetics & Heredity
Dongjie Zhang, Qian Zhang, Liang Wang, Jiaxin Li, Wanjun Hao, Yuanlu Sun, Di Liu, Xiuqin Yang
Summary: The study found that alternative splicing occurs in pig CREB genes, and two isoforms, V1 and V2, have similar roles but exhibit gene-specific effects. Additionally, V3 has no effect on gene expression, while V4 acts as a repressor. The different mechanisms underlying the roles of V1 and V2 were identified through RNA-seq, and specific genes regulated by them were identified.
Article
Fisheries
Qiang Luo, Xuefeng Zhou, Xing Lv, Weiwei Zheng, Shang Geng, Tianjun Xu, Yuena Sun
Summary: Alternative splicing is an important mechanism for controlling gene expression in eukaryotes. This study identified three different splicing isoforms in the ferritin gene fthl27 of Miichthys miiuy and found that they showed an upregulation trend after stimulation. These isoforms may have different regulatory effects on inflammatory factors and antiviral immune factors, playing a significant role in the innate immune response of fish.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kamila Kwiecien, Pawel Majewski, Maciej Bak, Piotr Brzoza, Urszula Godlewska, Izabella Skulimowska, Joanna Cichy, Mateusz Kwitniewski
Summary: This study identified novel transcript variants of mouse Rarres2 and found limited role for alternative splicing in generating chemerin isoform diversity, except in brown adipose tissue. Only one transcript variant of human RARRES2 was present in liver and adipose tissue.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Veterinary Sciences
Adam D. Naylor, Douglas Richardson, Morag Sellar, Jane Harley, Adrian W. Philbey, Simon J. Girling
JOURNAL OF ZOO AND WILDLIFE MEDICINE
(2018)
Article
Pathology
Maristela L. Onozato, Clarence Yapp, Douglas Richardson, Tilak Sundaresan, Varun Chahal, Jesse Lee, James P. Sullivan, Marisa W. Madden, Hyo S. Shim, Matthew Liebers, Quan Ho, Shyamala Maheswaran, Daniel A. Haber, Zongli Zheng, Brian Clancy, Hunter L. Elliott, Jochen K. Lennerz, A. John Iafrate
JOURNAL OF MOLECULAR DIAGNOSTICS
(2019)
Article
Pharmacology & Pharmacy
Constantin Mircioiu, Valentina Anuta, Ion Mircioiu, Adrian Nicolescu, Nikoletta Fotaki
Article
Biology
Claudia Prahst, Parham Ashrafzadeh, Thomas Mead, Ana Figueiredo, Karen Chang, Douglas Richardson, Lakshmi Venkaraman, Mark Richards, Ana Martins Russo, Kyle Harrington, Marie Ouarne, Andreia Pena, Dong Feng Chen, Lena Claesson-Welsh, Kin-Sang Cho, Claudio A. Franco, Katie Bentley
Editorial Material
Biochemistry & Molecular Biology
Yupeng Wang, Douglas S. Richardson
Article
Veterinary Sciences
Simon J. Girling, Romain Pizzi, Adam D. Naylor, Douglas Richardson, Una Richardson, Jane Harley, Georgina Cole, Donna Brown, Mary Fraser, Emma Tillman, David Barclay
JOURNAL OF ZOO AND WILDLIFE MEDICINE
(2020)
Review
Biochemical Research Methods
Erin E. Diel, Jeff W. Lichtman, Douglas S. Richardson
Correction
Biochemical Research Methods
Erin E. Diel, Jeff W. Lichtman, Douglas S. Richardson
Article
Oncology
Isidora Tosic, Lisa N. Heppler, Susana P. Egusquiaguirre, Natalie Boehnke, Santiago Correa, Daniel F. Costa, Elizabeth A. Grossman Moore, Sharmistha Pal, Douglas S. Richardson, Alexander R. Ivanov, Daphne A. Haas-Kogan, Daniel K. Nomura, Paula T. Hammond, David A. Frank
Summary: The oncogenic transcription factor STAT3 is aberrantly activated in breast cancers, leading to changes in cellular lipid distribution. By using layer-by-layer nanoparticles with different surface layers, targeted therapy can be achieved for STAT3-activated breast cancer cells, showing promising therapeutic effects.
MOLECULAR CANCER THERAPEUTICS
(2021)
Editorial Material
Pharmacology & Pharmacy
Constantin Mircioiu, Valentina Anuta, Momir Mikov, Adrian Nicolescu, Victor A. Voicu
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Amit A. Nagarkar, Samuel E. Root, Michael J. Fink, Alexei S. Ten, Brian J. Cafferty, Douglas S. Richardson, Milan Mrksich, George M. Whitesides
Summary: The study demonstrates that digital data can be stored in mixtures of fluorescent dye molecules on a surface, tethered by amide bonds. Individual dyes in the mixture can be distinguished using a microscope equipped with a fluorescence detector, encoding them into binary information. The use of molecular mixtures instead of sequence-defined macromolecules reduces synthesis time and eliminates sequencing requirements.
ACS CENTRAL SCIENCE
(2021)
Article
Pharmacology & Pharmacy
Victor Voicu, Marilena Jiquidi, Constantin Mircioiu, Roxana Sandulovici, Adrian Nicolescu
Summary: The study investigated the decorporation kinetics of internal radionuclide contamination and found that mono- and bi-compartmental decorporation kinetics models are applicable for rapid intervention, while a diffusion model of the radionuclide from tissues to blood is better for describing decorporation kinetics after more than ten days post treatment. The square-root law for describing the Zn-65 decorporation predicts a slower release rate than exponential equations and better explains the long persistence of radionuclides in the living body.
Article
Biology
Theodore J. Zwang, Rachel E. Bennett, Maria Lysandrou, Benjamin Woost, Anqi Zhang, Charles M. Lieber, Douglas S. Richardson, Bradley T. Hyman, Albert Cardona
Summary: In this manuscript, a procedure for rapidly designing immunostaining protocols for antibody labeling of cleared brain tissue is described. Libraries of fixed, pre-treated, and cleared tissue sections were prepared and used to optimize staining conditions for a panel of antibodies. The approach outlined here will help researchers successfully label a variety of targets, thus removing a major hurdle to accessing the rich 3D information available in large, cleared human tissue volumes.
Article
Clinical Neurology
Theodore J. Zwang, Benjamin Woost, Joshua Bailey, Zachary Hoglund, Douglas S. Richardson, Rachel E. Bennett, Bradley T. Hyman
Summary: Zwang et al. use tissue clearing techniques to image and characterize tau tangles, neurons, and nuclei across cortical layers. They identified dozens of ghost tangles, a rare event, and found that they are more abundant in layers three and five.
BRAIN COMMUNICATIONS
(2023)
Review
Multidisciplinary Sciences
Douglas S. Richardson, Webster Guan, Katsuhiko Matsumoto, Chenchen Pan, Kwanghun Chung, Ali Erturk, Hiroki R. Ueda, Jeff W. Lichtman
Summary: Tissue clearing technology has widespread applications and potential in the field of microscopy. This article outlines a modular approach for researchers to develop clearing processes, and provides overviews of certain applications, technological limitations, and future development prospects.
NATURE REVIEWS METHODS PRIMERS
(2021)