Article
Biochemistry & Molecular Biology
Katherine E. E. Stanford, Xiaohong Zhao, Nathan Kim, Daniel C. C. Masison, Lois E. E. Greene
Summary: The yeast Sup35 protein misfolds into the infectious [PSI+] prion, which is propagated by Hsp104. Unlike other yeast prions, this prion is efficiently cured by overexpression or inactivation of Hsp104. The extent of curing is not affected by asymmetric segregation or inhibition of cell division, indicating that Hsp104 overexpression leads to prion dissolution.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Qiao Wang, Xinren Dai, Hongying Pang, Yanxia Cheng, Xiong Huang, Hui Li, Xiaojing Yan, Fachuang Lu, Hairong Wei, Ronald R. Sederoff, Quanzi Li
Summary: This study focuses on the transcriptional regulation of the key enzyme CAld5H2 for sinapyl alcohol biosynthesis. Through yeast one-hybrid screen and ChIP-qPCR experiments, 12 upstream transcription factors (TFs) and a negative regulator BLH6a were identified. Luciferase complementation imaging analyses indicated extensive protein-protein interactions among these TFs, suggesting a combinatorial regulation for sinapyl alcohol biosynthesis in poplar.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Ren Wang, Ellie Himschoot, Matteo Grenzi, Jian Chen, Alaeddine Safi, Melanie Krebs, Karin Schumacher, Moritz K. Nowack, Wolfgang Moeder, Keiko Yoshioka, Daniel Van Damme, Ive De Smet, Danny Geelen, Tom Beeckman, Jiri Friml, Alex Costa, Steffen Vanneste
Summary: This study evaluates the effects of different auxin analogs on the accumulation of PIN proteins in BFA bodies and their ability to induce Ca2+ responses. The results show that not all auxin analogs can elicit Ca2+ responses and their ability to do so partially correlates with their inhibition of BFA body formation.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Immunology
Luhao Zhang, Xin Zhang, Jiaming Liao, Linting Xu, Shaozhu Kang, Hong Chen, Mengshi Sun, Siting Wu, Zhuqing Xu, Shina Wei, Qiwei Qin, Jingguang Wei
Summary: This study cloned and identified a cGAS homolog, EccGAS, from orange-spotted grouper. EccGAS was found to be abundantly expressed in the blood, skin, and gills, and it inhibited the production of type I interferon and interacted with other signaling molecules, suggesting it may serve as a negative regulator of the cGAS-STING signaling pathway in fish.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Kamilla M. E. Laidlaw, Katherine M. Paine, Daniel D. Bisinski, Grant Calder, Karen Hogg, Sophia Ahmed, Sally James, Peter J. O'Toole, Chris MacDonald
Summary: Cell surface protein trafficking is regulated in response to nutrient availability. Recycling of internalized protein and lipid cargoes is attenuated during glucose starvation, potentially due to changes in endosomal PI3K activity and the involvement of Gpa2.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Multidisciplinary Sciences
Jun Sun, Dong Yeon Shin, Mark Eiseman, Alisha R. Yallowitz, Na Li, Sarfaraz Lalani, Zan Li, Michelle Cung, Seoyeon Bok, Shawon Debnath, Sofia Jenia Marquez, Tommy E. White, Abdul G. Khan, Ivo C. Lorenz, Jae-Hyuck Shim, Francis S. Lee, Ren Xu, Matthew B. Greenblatt
Summary: Slitrk5 acts as a negative regulator of hedgehog signaling in osteoblasts by enhancing osteoblast differentiation and inhibiting hedgehog signaling downstream targets. Mechanistically, Slitrk5 interacts with hedgehog ligands via its extracellular domain and with PTCH1 via its intracellular domain, ultimately modulating hedgehog signaling activity in the primary cilium.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Yu Zhou, Yi Lei, Long-Feng Lu, Dan-Dan Chen, Can Zhang, Zhuo-Cong Li, Xiao-Yu Zhou, Shun Li, Yong-An Zhang
Summary: The study reveals that the cGAS orthologs from crucian carp and grass carp can negatively regulate the IFN response by targeting the RIG-I sensor, inhibiting IFN induction and antiviral response, thus facilitating viral replication.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marcel Albacar, Diego Velazquez, Antonio Casamayor, Joaquin Arino
Summary: Overexpression of protein phosphatase Ppz1 in Saccharomyces cerevisiae strongly inhibits cell growth. However, overexpression of its subunit Hal3 can completely counteract the toxic effects caused by excess Ppz1. Hal3 not only inhibits the enzymatic activity of Ppz1, but also recruits the phosphatase to internal structures.
Review
Biochemistry & Molecular Biology
Galina A. Zhouravleva, Stanislav A. Bondarev, Nina P. Trubitsina
Summary: The number of yeast prions and prion-like proteins has significantly increased since 1994. Initially, skepticism existed among scientists regarding the use of the term "prion" to describe yeast cytoplasmic elements, but now it is widely accepted that prion-like phenomena are common in yeast. This article provides an overview of yeast prions discovered so far and discusses different approaches used to identify them, as well as the potential for discovering new yeast prions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Lingjian Zhou, Xutong Xue, Ke Yang, Zhi Feng, Min Liu, Jose C. Pastor-Pareja
Summary: The study reveals that the trans-Golgi is the main location for lysosomes in Drosophila cells, where basal microautophagy of secretory cargos normally takes place, and contributes to autophagic vesicle formation under stress conditions.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
James Osei-Owusu, Junhua Yang, Ka Ho Leung, Zheng Ruan, Wei Lu, Yamuna Krishnan, Zhaozhu Qiu
Summary: The proton-activated Cl- (PAC) channel has been identified as a low pH sensor in endosomes, preventing hyper-acidification by releasing Cl- from the lumen.
Article
Multidisciplinary Sciences
Karin Vogel, Tobias Blaeske, Marie-Kristin Nagel, Christoph Globisch, Shane Maguire, Lorenz Mattes, Christian Gude, Michael Kovermann, Karin Hauser, Christine Peter, Erika Isono
Summary: This study identified two plasma membrane-localized deubiquitylating enzymes (DUBs) in Arabidopsis that are involved in the endocytic transport and degradation of plasma membrane proteins by binding to anionic lipids. The results highlight the importance of OTU11 and OTU12 in the fine-tuning of plasma membrane proteins in Arabidopsis.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Hongbo Li, Ruihua Huang, Yanglan Liao, Shuhong Yang, Bojin Feng, Hongting Qin, Jun Zhou, Yonglun Zeng, Jinbo Shen, Xiaohong Zhuang, Liwen Jiang, Marisa S. Otegui, Shengchun Zhang, Caiji Gao
Summary: The study identifies a plant-specific endosomal regulator, BLISTER (BLI), that interacts with the retromer complex and plays a crucial role in regulating endosomal trafficking in plants.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Bryan Grimes, Walter Jacob, Amanda R. Liberman, Nathan Kim, Xiaohong Zhao, Daniel C. Masison, Lois E. Greene
Summary: The Sup35 prion protein of budding yeast can undergo phase separation to form liquid droplets, which requires the involvement of the C-terminal domain and is related to stress granules. Additionally, the phase separation of Sup35 appears to be dependent on the presence of mRNA, and the condensates formed in vivo do not disassemble when the intracellular pH is increased.
Article
Biochemistry & Molecular Biology
Kohta Takahashi, Kristiina Kanerva, Lauri Vanharanta, Leonardo Almeida-Souza, Daniel Lietha, Vesa M. Olkkonen, Elina Ikonen
Summary: The study reveals the mechanism by which ORP2 regulates lipid and protein trafficking in cells, controlling FAK activation and LDL-cholesterol plasma membrane delivery by facilitating cholesterol and PI(4,5)P-2 exchange between late and recycling endosomes.