Article
Chemistry, Organic
Kalyana Kumar Ponnapalli, Yi-Chi Ho, Mei-Chun Tseng, Bhanu Vijaya Sekhar Vasamsetti, Jiun-Jie Shie
Summary: This study describes a double one-pot strategy for the synthesis of N-glycans, assisted by an IM-MS analysis approach for rapid screening of optimized glycosylation reaction conditions. The method enables the efficient synthesis of high-mannose type N-glycans with excellent stereo- and regioselectivities.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Rosa L. Cordeiro, Camila R. Santos, Mariane N. Domingues, Tatiani B. Lima, Renan A. S. Pirolla, Mariana A. B. Morais, Felippe M. Colombari, Renan Y. Miyamoto, Gabriela F. Persinoti, Antonio C. Borges, Marcelo A. de Farias, Fabiane Stoffel, Chao Li, Fabio C. Gozzo, Marin van Heel, Marcelo E. Guerin, Eric J. Sundberg, Lai-Xi Wang, Rodrigo V. Portugal, Priscila O. Giuseppe, Mario T. Murakami
Summary: This study elucidated the key biochemical steps and molecular mechanisms by which Bifidobacterium longum utilizes high-mannose N-glycans, providing insights into the utilization of this perennial carbon and energy source in the intestinal lumen.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Medicine, Research & Experimental
You Zhuo, David A. Keire, Kang Chen
Summary: The N-glycosylation pattern of Asn-297 can impact mAb drug plasma clearance, ADCC, and CDC. Using a middle-down NMR spectroscopy based analytical procedure, the composition and structure of glycans on adalimumab and trastuzumab were assessed without glycan cleavage. The obtained information is relevant for assessing mAb quality attributes.
MOLECULAR PHARMACEUTICS
(2021)
Article
Physics, Multidisciplinary
H. L. Crawford, V Tripathi, J. M. Allmond, B. P. Crider, R. Grzywacz, S. N. Liddick, A. Andalib, E. Argo, C. Benetti, S. Bhattacharya, C. M. Campbell, M. P. Carpenter, J. Chan, A. Chester, J. Christie, B. R. Clark, I. Cox, A. A. Doetsch, J. Dopfer, J. G. Duarte, P. Fallon, A. Frotscher, T. Gaballah, T. J. Gray, J. T. Harke, J. Heideman, H. Heugen, R. Jain, T. T. King, N. Kitamura, K. Kolos, F. G. Kondev, A. Laminack, B. Longfellow, R. S. Lubna, S. Luitel, M. Madurga, R. Mahajan, M. J. Mogannam, C. Morse, S. Neupane, A. Nowicki, T. H. Ogunbeku, W. -j. Ong, C. Porzio, C. J. Prokop, B. C. Rasco, E. K. Ronning, E. Rubino, T. J. Ruland, K. P. Rykaczewski, L. Schaedig, D. Seweryniak, K. Siegl, M. Singh, S. L. Tabor, T. L. Tang, T. Wheeler, J. A. Winger, Z. Xu
Summary: New half-lives for exotic isotopes approaching the neutron drip-line were measured and compared to theoretical models. The results suggest a well-developed region of deformation in N=28 isotones and confirm the weakening of subshell closure in Si with Z=14. The observed reduction in the half-life of 38Mg compared to its neighbors highlights the need for more advanced theoretical efforts.
PHYSICAL REVIEW LETTERS
(2022)
Article
Chemistry, Physical
Ana Garcia-Garcia, Sonia Serna, Zhang Yang, Ignacio Delso, Victor Taleb, Thomas Hicks, Raik Artschwager, Sergey Y. Vakhrushev, Henrik Clausen, Jesus Angulo, Francisco Corzana, Niels C. Reichardt, Ramon Hurtado-Guerrero
Summary: Core fucosylation by FUT8 is affected by the underlying peptide sequence for paucimannose and high-mannose N-glycans, but not for complex-type N-glycans. FUT8 recognizes all sugar units and most amino acid residues of the G0 N-glycan, with optimal recognition requiring prior binding to GDP-Fuc. Genetic engineering in CHO cells demonstrated that core fucosylation mainly occurs on complex-type N-glycans, but certain glycosites can acquire Fuc on high-mannose N-glycans.
Article
Forestry
Katarina Razna, Jana Ziarovska, Eva Ivanisova, Lucia Urbanova, L'ubomir Harencar, Adam Kovacik, Matus Kucka, Pavel Hrubik
Summary: Through research on different species of lime-tree flowers, it was found that miRNA markers can be used to determine the polymorphism of plant flowers, and it was also discovered that silver linden flowers have the highest content of bioactive compounds and antioxidant activity.
Article
Biotechnology & Applied Microbiology
Jichen Chen, Hong Du, Zidong Liu, Tangcheng Li, Hua Du, Wanna Wang, Muhammad Aslam, Weizhou Chen, Ping Li, Haodong Luo, Hao Fang, Xiaojuan Liu
Summary: This study investigated the effects of tunicamycin stress on the marine diatom Phaeodactylum tricornutum. The results showed that tunicamycin stress significantly inhibited cell growth and photosynthesis, and caused alterations in carbon and nitrogen metabolisms. Additionally, the study enriched the N-glycan database of P. tricornutum and provided important information for studying the function of N-glycosylation modification on proteins.
MICROBIAL CELL FACTORIES
(2022)
Article
Environmental Sciences
Daniel E. Dawson, Christopher Lau, Prachi Pradeep, Risa R. Sayre, Richard S. Judson, Rogelio Tornero-Velez, John F. Wambaugh
Summary: In this study, a random forest method was used to model the t(1/2) of PFAS across four species and eleven compounds. A classification model for t(1/2) was developed, and the t(1/2) of 3890 compounds was predicted. This model provides a basis for tentative extrapolation and prioritization.
Article
Physics, Nuclear
C. Ma, Y. Lu, Y. Lei, Y. M. Zhao
Summary: In this paper, we systematically calculate the n-decay half-lives of waiting-point isotones with N = 81 and 82 in the r-process using the nucleon-pair approximation. A phenomenological form of the Hamiltonian, including single-particle energies, pairing plus quadrupole interactions, and Gamow-Teller interactions, is assumed. Theoretical n-decay energies are derived based on Bayesian machine learning, and a good agreement with experimental values is achieved by using the nucleon-pair truncated model space dominated by S-pair condensation.
Article
Multidisciplinary Sciences
Shiva Izadi, Mokhtar Jalali Javaran, Sajad Rashidi Monfared, Alexandra Castilho
Summary: Thrombolytic and fibrinolytic therapies are effective but face challenges in protein stability. By mutating critical residues, stable monomeric fusion proteins were successfully expressed, showing the ability to dissolve blood clots effectively. Strategies to improve glycosylation towards serum-like structures in plant-based expression systems were also addressed in this study.
Article
Biochemistry & Molecular Biology
Ala' Abu-Odeh, Mayadah Shehadeh, Ghadeer A. R. Y. Suaifan, Nida Karameh, Diana Abdel Rahman, Yasser Kandil
Summary: This study evaluated the blood-glucose-lowering potential of Terfezia claveryi truffle extracts and found that the aqueous extracts exhibited α-glucosidase inhibitory effect in vitro and significant antihyperglycemic activity in a diabetic mice model.
Article
Physics, Nuclear
R. Gharaei, S. Mohammadi, Dashty T. Akrawy, Ali H. Ahmed
Summary: In this study, 16 different versions of proximity potentials were used to investigate the alpha decay half-lives of 21 possible isotopes around N = Z in the range of 52 ≤ Z ≤ 56. The results showed that the theoretical half-lives of the Prox. 81 and Prox. 88 potential models were in close agreement with experimental data. The study also discussed the variation of the penetration probability of alpha particles and alpha decay half-lives with neutron content based on the isotopic dependence of the Coulomb potential barrier and the released energy. Additionally, the present approaches were compared with other empirical and semi-empirical formulae, and the SemFIS formula was found to have the lowest standard deviation among them for the alpha decay of the studied nuclei.
EUROPEAN PHYSICAL JOURNAL A
(2022)
Article
Chemistry, Medicinal
Zhishan Xu, Mengke Xu, Xueya Wu, Sheng Guo, Zhongwei Tian, Di Zhu, Jixuan Yang, Jiyun Fu, Xi Li, Guozhen Song, Zhe Liu, Xiangfeng Song
Summary: This study reports a half-sandwich complex Ru(II) with aryl-bis(imino) acenaphthene chelating ligand, which induces immunogenic cell death (ICD) in melanoma in vitro and in vivo. The complex displays strong anti-proliferative and potential cell migration inhibition effects against melanoma cell lines. Furthermore, the complex drives the biochemical hallmarks of ICD in melanoma cells, leading to the activation of adaptive immune responses and anti-tumor immunity.
Article
Environmental Sciences
Elisa Terzaghi, Elena Alberti, Giuseppe Raspa, Elisabetta Zanardini, Cristiana Morosini, Simone Anelli, Stefano Armiraglio, Antonio Di Guardo
Summary: This paper presents a new dataset of PCBs half-lives in soil, showing the potential of different plant species and soil conditions to stimulate biodegradation of PCBs. The obtained half-lives ranged from 1.3 to 5.6 years, indicating the importance of cultivation and plant-microbe interactions in speeding up PCB biodegradation and improving soil remediation predictions.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Microbiology
Lifeng Zhu, Qinnan Yang, Mallory J. Suhr Van Haute, Car Reen Kok, Joao Carlos Gomes-Neto, Natasha Pavlovikj, Resmi Pillai, Rohita Sinha, Haley Hassenstab, Aaryn Mustoe, Etsuko N. Moriyama, Robert Hutkins, Jeffrey French, Andrew K. Benson
Summary: The study characterized the fecal microbiomes of common marmosets and found that adult animals were dominated by certain species of Bifidobacterium. The research also identified several species of Bifidobacterium that are abundant members of the captive marmoset microbiome throughout their life history. Genomic analysis suggested species-specific adaptations in Bifidobacterium to different components of the captive marmoset diet.
Article
Biochemistry & Molecular Biology
Linda J. Olson, Ramiro Orsi, Francis C. Peterson, Armando J. Parodi, Jung-Ja P. Kim, Cecilia D'Alessio, Nancy M. Dahms
Article
Plant Sciences
Francisca Blanco-Herrera, Adrian A. Moreno, Rodrigo Tapia, Francisca Reyes, Macarena Araya, Cecilia D'Alessio, Armando Parodi, Ariel Orellana
Review
Biochemistry & Molecular Biology
Cecilia D'Alessio, Nancy M. Dahms
CURRENT PROTEIN & PEPTIDE SCIENCE
(2015)
Article
Biochemistry & Molecular Biology
Luis M. Bredeston, Cristina Marino-Buslje, Vanesa S. Mattera, Lucila I. Buzzi, Armando J. Parodi, Cecilia D'Alessio
Article
Multidisciplinary Sciences
Leonardo M. Diaz-Nieto, Cecilia D'Alessio, M. Alejandra Perotti, Corina M. Beron
Article
Cell Biology
Mariana Melani, Ayelen Valko, Nuria M. Romero, Milton O. Aguilera, Julieta M. Acevedo, Zambarlal Bhujabal, Joel Perez-Perri, Rocio V. de la Riva-Carrasco, Maximiliano J. Katz, Eleonora Sorianello, Cecilia D'Alessio, Gabor Juhasz, Terje Johansen, Maria I. Colombo, Pablo Wappner
MOLECULAR BIOLOGY OF THE CELL
(2017)
Article
Biophysics
Rodrigo S. Pagano, Maximo Lopez Medus, Gabriela E. Gomez, Paula M. Couto, Maria S. Labanda, Lucas Landolfo, Cecilia D'Alessio, Julio J. Caramelo
BIOPHYSICAL JOURNAL
(2014)
Article
Biochemistry & Molecular Biology
Linda J. Olson, Ramiro Orsi, Solana G. Alculumbre, Francis C. Peterson, Ivan D. Stigliano, Armando J. Parodi, Cecilia D'Alessio, Nancy M. Dahms
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Multidisciplinary Sciences
Natalia Y. Boynak, Federico Rojas, Cecilia D'Alessio, Salome C. Vilchez Larrea, Vanina Rodriguez, Pablo D. Ghiringhelli, Maria T. Tellez-Inon
Article
Biochemistry & Molecular Biology
Giovanna L. Gallo, Ayelen Valko, Sofia I. Aramburu, Emiliana Etchegaray, Christof Voelker, Armando J. Parodi, Cecilia D'Alessio
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Multidisciplinary Sciences
Claudia R. Arbeitman, Gabriela Auge, Matias Blaustein, Luis Bredeston, Enrique S. Corapi, Patricio O. Craig, Leandro A. Cossio, Liliana Dain, Cecilia D'Alessio, Fernanda Elias, Natalia B. Fernandez, Yamila B. Gandola, Javier Gasulla, Natalia Gorojovsky, Gustavo E. Gudesblat, Maria G. Herrera, Lorena I. Ibanez, Tommy Idrovo, Matias Iglesias Rando, Laura Kamenetzky, Alejandro D. Nadra, Diego G. Noseda, Carlos H. Pavan, Maria F. Pavan, Maria F. Pignataro, Ernesto Roman, Lucas A. M. Ruberto, Natalia Rubinstein, Javier Santos, Francisco Velazquez, Alicia M. Zelada
SCIENTIFIC REPORTS
(2020)
Article
Cell Biology
Giovanna L. Gallo, Ayelen Valko, Nathalia Herrera Aguilar, Ariel D. Weisz, Cecilia D'Alessio
Summary: In this study, a platform of Schizosaccharomyces pombe mutants was constructed to investigate the molecular basis of Congenital Disorders of Glycosylation type I (CDG-I). The results showed that the presence of glucose (Glc) in lipid-linked oligosaccharides (LLOs) is more relevant to the transfer efficiency than the number of mannose (Man) residues in S. pombe. Surprisingly, a decrease in the number of Man residues in glycans improved the glycan transfer. Furthermore, expressing a single-subunit oligosaccharyltransferase with broad substrate specificity could revert the deficiency of glycosylation.
JOURNAL OF CELL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Paula M. Berguer, Matias Blaustein, Luis M. Bredeston, Patricio O. Craig, Cecilia D'Alessio, Fernanda Elias, Paola C. Farre, Natalia B. Fernandez, Hernan G. Gentili, Yamila B. Gandola, Javier Gasulla, Gustavo E. Gudesblat, Maria G. Herrera, Lorena I. Ibanez, Tommy Idrovo-Hidalgo, Alejandro D. Nadra, Diego G. Noseda, Carlos H. Pavan, Maria F. Pavan, Maria F. Pignataro, Ernesto A. Roman, Lucas A. M. Ruberto, Natalia Rubinstein, Maria Sanchez, Javier Santos, Diana E. Wetzler, Alicia M. Zelada
Summary: In this study, multimeric particles composed of the receptor binding domain (RBD) of SARS-CoV-2 Spike protein covalently coupled to Lumazine Synthase from Brucella abortus were produced. These particles demonstrated significantly higher humoral immune response and induced a high titer of neutralizing antibodies. Thus, multimeric RBD-BLS particles are promising candidates for a protein-based vaccine.
SCIENTIFIC REPORTS
(2022)
Article
Biology
Ayelen Valko, Giovanna L. Gallo, Ariel D. Weisz, Armando J. Parodi, Cecilia D'Alessio
Summary: This article presents a protocol for determining the LLO patterns produced by S. pombe cells, and provides a simplified method for analyzing paper chromatograms. The method is cost-effective and can be applied to other cell types.