Article
Microbiology
Sreekumar Othumpangat, John D. Noti
Summary: Understanding the host response to influenza A virus (IAV) infection is crucial for developing intervention strategies. Antimicrobial protein beta-defensin-1 plays a key role in regulating IAV survival through the STAT3 pathway. Overexpression of DEFB1 in human bronchial epithelial cells resulted in a significant reduction in IAV replication. This suggests that beta-defensin-1 could be a potential target for antiviral drug development.
Article
Immunology
Rowayna Shouib, Gary Eitzen
Summary: Airway epithelial cells can respond to pathogens and stimulants through cytokine and chemokine secretion, but excessive secretion can cause chronic inflammation and pulmonary disorders. We identified Cdc42 as an important signaling molecule that regulates cytokine production and release. Inhibiting or silencing Cdc42 affected the gene expression and secretion of specific cytokines, particularly IL-8 trafficking and secretion.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tomoki Yano, Kazuto Tsukita, Hatsuho Kanoh, Shogo Nakayama, Hiroka Kashihara, Tomoaki Mizuno, Hiroo Tanaka, Takeshi Matsui, Yuhei Goto, Akira Komatsubara, Kazuhiro Aoki, Ryosuke Takahashi, Atsushi Tamura, Sachiko Tsukita
Summary: This study identified a system triggered by ppMLC at TJ-associated CRs for vertebrate apical constriction, involving microtubules, LUZP1, and myosin phosphatase. LUZP1 was found to inhibit myosin phosphatase at TJ-associated CRs in a microtubule-facilitated manner, significantly contributing to the understanding of apical constriction in vertebrates.
Article
Medicine, Research & Experimental
Xiaobo Zhang, Jun Xie, Hongmei Sun, Qin Wei, Guangmin Nong
Summary: Research on neutrophilic asthma (NA) suggests that decreased miR-29a-3p and elevated SPARC levels are associated with EMT in the mouse model, where SPARC induces EMT in 16HBE cells and miR-29a-3p can regulate this process.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Qiwen Shi, Bei Hu, Chen Yang, Lan Zhao, Jing Wu, Nan Qi
Summary: Arsenic is a common environmental pollutant that can also be used as a chemotherapeutic agent for acute promyelocytic leukemia. The relationship between arsenic exposure and lung diseases is established, but the molecular mechanisms are poorly understood. Research shows that ATF3 plays a pro-apoptotic role in arsenic-induced airway epithelial apoptosis by transcriptionally regulating DR5 and Bcl-xL, highlighting its potential as an early and sensitive biomarker for arsenic-induced lung injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Tomohiro Ogawa, Keiko Kan-o, Ayaka Shiota, Akitaka Fujita, Yumiko Ishii, Satoru Fukuyama, Koichiro Matsumoto
Summary: This study found that during viral infections, antiviral interferons can induce the expression of PD-L1 and PD-L2 in bronchial epithelial cells. By inhibiting the PI3K delta signaling pathway, the expression of these two immune checkpoint molecules can be differentially regulated in bronchial epithelial cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biology
Jia Chen, Daniel St Johnston
Summary: In the adult Drosophila midgut, the process of enteroblast integration resembles lumen formation in mammalian epithelial cysts, with enteroblasts forming a pre-assembled apical compartment and an apical membrane initiation site (AMIS) before integrating into the epithelium.
Article
Genetics & Heredity
Xirui Ma, Huifang Liu, Jing Zhu, Caoxu Zhang, Yajie Peng, Ziming Mao, Yu Jing, Fengling Chen
Summary: MiR-185-5p has been identified as a negative regulator in pro-inflammatory activation of macrophages, exerting anti-inflammatory effects through the CDC42/JNK pathway.
Article
Dentistry, Oral Surgery & Medicine
Y. Li, J. Zhang, W. Cai, C. Wang, Z. Yu, Z. Jiang, K. Lai, Y. Wang, G. Yang
Summary: The long-term success rate of dental implants can be improved by establishing a favorable biological sealing with a high-quality epithelial attachment. The application of mesenchymal stem cells (MSCs) holds promise for facilitating the soft tissue integration around implants, but the molecular mechanism is still unclear and the general application of MSC sheet for soft tissue integration is also relatively unexplored.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Biology
Peng Shang, Nadezda Stepicheva, Kenneth Teel, Austin McCauley, Christopher Scott Fitting, Stacey Hose, Rhonda Grebe, Meysam Yazdankhah, Sayan Ghosh, Haitao Liu, Anastasia Strizhakova, Joseph Weiss, Imran A. Bhutto, Gerard A. Lutty, Ashwath Jayagopal, Jiang Qian, Jose-Alain Sahel, J. Samuel Zigler, James T. Handa, Yuri Sergeev, Raju V. S. Rajala, Simon Watkins, Debasish Sinha
Summary: The study highlights the crucial role of beta A3/A1-crystallin in the retinal pigmented epithelium, with its deficiency causing EGFR endocytosis defects and actin network disruption leading to polarity disruption and degeneration through the PITP beta/PLC signaling pathway.
COMMUNICATIONS BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Meng-Yu Zhang, Ying-Xiao Jiang, Yi-Can Yang, Jian-Yu Liu, Chen Huo, Xiu-Li Ji, Yi-Qing Qu
Summary: The study found that cigarette smoke induces inflammation and promotes pyroptosis in 16HBE cells through the ROS/NLRP3/caspase-1 pathway.
Article
Cell Biology
Beibei Guo, Mengwei Qi, Shuai Huang, Run Zhuo, Wenxue Zhang, Yufang Zhang, Man Xu, Mei Liu, Tuchen Guan, Yan Liu
Summary: The study identified Cadherin-12 (CDH12) as a gene that promotes neurite outgrowth by affecting Rac1/Cdc42 phosphorylation. This research provides a preliminary explanation for the mechanism of CDH12 in promoting axonal extension in vitro and in vivo.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Jin Wang, Xin Tian, Jie Zhang, Lirong Tan, Nan Ouyang, Beibei Jia, Chunying Chen, Cuicui Ge, Jianxiang Li
Summary: Long-term pulmonary exposure to carbon nanotubes (CNTs) can induce carcinogenesis and tumor metastasis. A study on human lung cells exposed to single-walled carbon nanotubes (SWCNTs) showed that postchronic exposure led to increased migration and invasion abilities, irreversible malignant transformation, and promotion of carcinogenesis in vivo through DNA methylation and transcriptome dysregulation.
Article
Environmental Sciences
Lin Xu, Qianwen Zhao, Daochuan Li, Jiao Luo, Wanli Ma, Yuan Jin, Chuanhai Li, Jing Chen, Kunming Zhao, Yuxin Zheng, Dianke Yu
Summary: This study revealed that hsa-miR-760 plays an important role in protecting lung cells against PM2.5-induced toxicity by upregulating HMOX1 expression through targeting the coding region and interacting with YBX1 protein, providing new insights into the diverse functions of miRNAs.
ENVIRONMENTAL POLLUTION
(2021)
Article
Environmental Sciences
Feng Yu, Xia Zhang, Lei Gao, Hainan Xue, Ling Liu, Suhua Wang, Shijie Chen, Lihua Huang
Summary: This study investigated the function and mechanism of long-chain non-coding RNAs in NPsNd(2)O(3)-induced airway inflammation, revealing that lncRNA loc105377478 promotes NF-kappa B activation by negatively regulating ADIPOR1 expression and upregulating IL-6 and IL-8 in 16HBE cells treated with NPs-Nd2O3.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biology
Joanne Durgan, Yun-Yu Tseng, Jens C. Hamann, Marie-Charlotte Domart, Lucy Collinson, Alan Hall, Michael Overholtzer, Oliver Florey
Review
Biochemistry & Molecular Biology
J. Durgan, O. Florey
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Joanne Durgan, Peter J. Parker
BIOCHEMICAL JOURNAL
(2010)
Article
Biochemistry & Molecular Biology
Joanne Durgan, Guangbo Tao, Matthew S. Walters, Oliver Florey, Anja Schmidt, Vanessa Arbelaez, Neal Rosen, Ronald G. Crystal, Alan Hall
Article
Biochemistry & Molecular Biology
Joanne Durgan, Noriko Kaji, Dan Jin, Alan Hall
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Immunology
Oliver Florey, Joanne Durgan, William Muller
JOURNAL OF IMMUNOLOGY
(2010)
Article
Biochemistry & Molecular Biology
Xiaojian Xu, Dan Jin, Joanne Durgan, Alan Hall
MOLECULAR AND CELLULAR BIOLOGY
(2013)
Article
Cell Biology
Yongchan Lee, Jens C. Hamann, Mark Pellegrino, Joanne Durgan, Marie-Charlotte Domart, Lucy M. Collinson, Cole M. Haynes, Oliver Florey, Michael Overholtzer
Article
Biochemistry & Molecular Biology
Joanne Durgan, Alf H. Lystad, Katherine Sloan, Sven R. Carlsson, Michael I. Wilson, Elena Marcassa, Rachel Ulferts, Judith Webster, Andrea F. Lopez-Clavijo, Michael J. Wakelam, Rupert Beale, Anne Simonsen, David Oxley, Oliver Florey
Summary: Autophagy is a crucial cellular process mediated by ATG8 protein lipidation to PE or PS, which are differentially delipidated by the ATG4 family, showing distinct cellular dynamics; this reveals important molecular differences in the autophagy pathway.
Editorial Material
Cell Biology
Joanne Durgan, Oliver Florey
Summary: The alternative conjugation of Atg8-family proteins to PS and PE in CASM is responsive to various cellular stimuli. Regulation of conjugation to PE and PS by ATG4s varies, along with altered dynamics between the two species. The discovery of alternative Atg8-family protein PS lipidation raises exciting new questions on their roles, regulation, and biology during non-canonical autophagy.
Article
Cell Biology
Jose L. Nieto-Torres, Joanne Durgan, Anais Franco-Romero, Paolo Grumati, Carlos M. Guardia, Andrew M. Leidal, Michael A. Mandell, Christina G. Towers, Fei Wang
Summary: The AIM Center organized a virtual eSymposium during the COVID-19 pandemic in 2020, providing a platform for early-career investigators to showcase their research in autophagy. The event was a learning experience for all involved, discussing the pros and cons of virtual meeting formats.
JOURNAL OF CELL SCIENCE
(2021)
Article
Cell Biology
Kirsty M. Hooper, Elise Jacquin, Taoyingnan Li, Jonathan M. Goodwin, John H. Brumell, Joanne Durgan, Oliver Florey
Summary: This study reveals the important role of V-ATPase in non-canonical autophagy, as it is sufficient to activate CASM. V-ATPase recruits ATG16L1 during LAP, STING- and drug-induced CASM, indicating a common mechanism. Key molecular players, including NADPH oxidase/ROS, converge on V-ATPase during LAP. The study also demonstrates the contribution of CASM to the Salmonella host response and the disruption of V-ATPase-ATG16L1 axis by SopF.
JOURNAL OF CELL BIOLOGY
(2022)
Review
Multidisciplinary Sciences
Joanne Durgan, Oliver Florey
Summary: Autophagy is a fundamental catabolic process coordinated by a network of autophagy-related proteins. The noncanonical autophagy pathway, which utilizes the same proteins, plays a crucial role in immunity, inflammation, cancer, and neurodegeneration. This review discusses the various stimuli and processes involved in noncanonical autophagy, as well as the molecular features of the associated pathways. The authors propose an updated and unified mechanism for noncanonical autophagy.
Article
Cell Biology
Joanne Durgan, Marta Rodriguez-Martinez, Brendan Rouse
Summary: Scientific research has a significant environmental impact, using a large amount of energy and resources. Scientists have a crucial role in addressing climate change and ecological crises, but may not fully realize the contribution of their research and work-related activities to emissions and pollution. In light of the ongoing consequences of climate change and ecological breakdown, scientists need to take urgent action to promote sustainable solutions. This article explores how research organizations can reduce their environmental footprint, share resources, and encourage global engagement in sustainable science.
IMMUNOLOGY AND CELL BIOLOGY
(2023)