Review
Neurosciences
Samuel W. Du, Krzysztof Palczewski
Summary: MicroRNAs are short, evolutionarily conserved noncoding RNAs that are critical for controlling normal cellular physiology. Recent research has highlighted their role in supporting retina function through regulating RPE activities. They have potential applications as therapeutics and biomarkers for visual diseases.
TRENDS IN NEUROSCIENCES
(2022)
Article
Multidisciplinary Sciences
Kakkad Regha, Mayuri Bhargava, Abdurrahmaan Al-Mubaarak, Chou Chai, Bhav Harshad Parikh, Zengping Liu, Claudine See Wei Wong, Walter Hunziker, Kah Leong Lim, Xinyi Su
Summary: Retinal pigment epithelial (RPE) cell transplantation is a promising option for treating age-related macular degeneration. Clinical grade stem-cell derived RPE requires efficient in vitro differentiation and purification methods. Different stem cell sources require customized purification strategies for RPE. Among the five purification methods compared, flow cytometry-based approach is suitable for isolating RPE from cultures with highly adherent non-RPE cells, although with lower yield. A combination of enzymatic and manual purification is ideal for clinical applications based on high purity and relatively short processing time.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Niina Bhattarai, Niina Piippo, Sofia Ranta-aho, Yashavanthi Mysore, Kai Kaarniranta, Anu Kauppinen
Summary: Resvega has been shown to improve cell membrane integrity, reduce LDH leakage, and decrease NLRP3 release, caspase-1 activity, and inflammatory cytokine secretion in IL-1 alpha-primed ARPE-19 cells. These results suggest that Resvega may potentially reduce NLRP3 inflammasome-mediated inflammation in RPE cells with impaired protein clearance.
Article
Multidisciplinary Sciences
Haibin Tian, Zhiyang Chen, Xiaoman Zhu, Qingjian Ou, Zhe Wang, Binxin Wu, Jing-Ying Xu, Caixia Jin, Furong Gao, Juan Wang, Jingfa Zhang, Jieping Zhang, Lixia Lu, Guo-Tong Xu
Summary: This study demonstrates that transforming RPE cells with anti-EMT properties can avoid the risk of EMT after transplantation and may provide a suitable candidate for AMD treatment.
Article
Biochemistry & Molecular Biology
Luigi Donato, Concetta Scimone, Simona Alibrandi, Sergio Zaccaria Scalinci, Carmela Rinaldi, Rosalia D'Angelo, Antonina Sidoti
Summary: This study demonstrates for the first time the dynamic RNA editome profiles in human retinal pigment epithelium cells, shedding more light on new mechanisms underlying retinal degeneration.
Article
Biochemistry & Molecular Biology
Parameswaran Gangadharan Sreekumar, Feng Su, Christine Spee, Elise Hong, Ravikiran Komirisetty, Eduardo Araujo, Steven Nusinowitz, Srinivasa Reddy, Ram Kannan
Summary: Oxidative stress plays a crucial role in the development of AMD, and PON2 has been found to have a beneficial role in preserving retinal function and protecting against mitochondrial dysfunction.
Article
Cell Biology
Mi Zhou, Yuanjun Zhao, Sarah R. Weber, Christopher Gates, Nicholas J. Carruthers, Han Chen, Xiaoming Liu, Hong-Gang Wang, Michael Ford, Matthew T. Swulius, Alistair J. Barber, Stephanie L. Grillo, Jeffrey M. Sundstrom
Summary: This study found that the expression of R345W-Fibulin-3 mutation alters the size and cargo of extracellular vesicles (EVs), which are sufficient to enhance cell migration in a TGF beta-dependent manner. These findings suggest that EV-bound TGF beta plays a critical role in inducing epithelial-mesenchymal transition (EMT) in retinal pigment epithelial (RPE) cells.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Cell Biology
Mi Zhou, Yuanjun Zhao, Sarah R. Weber, Christopher Gates, Nicholas J. Carruthers, Han Chen, Xiaoming Liu, Hong-Gang Wang, Michael Ford, Matthew T. Swulius, Alistair J. Barber, Stephanie L. Grillo, Jeffrey M. Sundstrom
Summary: The expression of R345W-Fibulin-3 alters the size and cargo of extracellular vesicles (EVs), which are sufficient to enhance cell migration and induce epithelial-mesenchymal transition (EMT) in recipient cells. Transforming growth factor beta (TGF beta) plays a critical role in EV-mediated EMT induction.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Biochemistry & Molecular Biology
Blanca Chinchilla, Rosario Fernandez-Godino
Summary: Changes in the Bruch's membrane can lead to age-related macular degeneration, with abnormal ECM leading to epithelial mesenchymal transition in RPE cells. Understanding these processes may lead to the development of new therapies for AMD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Lujia Feng, Liang Liang, Shaochong Zhang, Jinglu Yang, Yanan Yue, Xuedong Zhang
Summary: This study investigated the relationship between HMGB1, the macroautophagy/autophagy-lysosome pathway, and DR, finding that high glucose inhibited autophagy and diminished its degradative capacity due to lysosome membrane permeabilization. HMGB1 was involved in lysosome membrane permeabilization via the CTSB-dependent pathway in the early stages of DR.
Review
Cell Biology
Yuxiang Du, Bo Yan
Summary: The retinal pigment epithelium is crucial for maintaining ocular immune privilege by using various mechanisms to suppress immune responses and prevent inflammation. This review focuses on the role of retinal pigment epithelium cells in immune regulation, particularly the changes that occur under pathological conditions.
JOURNAL OF LEUKOCYTE BIOLOGY
(2023)
Article
Ophthalmology
Yi Chen, Binxin Wu, Jian Feng He, Jingyao Chen, Zi Wei Kang, Dandan Liu, Junjie Luo, Kexin Fang, Xiaoxu Leng, Haibin Tian, Jingying Xu, Caixia Jin, Jieping Zhang, Juan Wang, Jingfa Zhang, Qingjian Ou, Lixia Lu, Furong Gao, Guo-Tong Xu
Summary: This study successfully induced EMT in RPE cells through prolonged low-density culture, and found that the combination of the ROCK inhibitor Y27632 and the TGF-beta receptor inhibitor RepSox effectively suppressed and reversed the EMT process, maintaining the morphology and function of RPE cells and the retina. This combined therapy could be a new strategy for treating proliferative retinal diseases involving EMT of RPE cells.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Jongmin Kim, Ju Young Park, Jeong Sik Kong, Hyungseok Lee, Jae Yon Won, Dong Woo Cho
Summary: The retinal pigment epithelium (RPE) plays a crucial role in retinal degenerative diseases, but there is currently no cure. Research has shown that bioink derived from porcine Bruch's membrane can enhance RPE functionality, and a Bruch's membrane-mimetic substrate (BMS) developed using 3D printing technology can facilitate appropriate RPE functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Arnau Navines-Ferrer, Sheila Ruiz-Nogales, Rafael Navarro, Esther Pomares
Summary: This study investigated the correlation between the p.Pro77Ser mutation in the BEST1 gene and the ocular phenotype in patients with Best Vitelliform Macular dystrophy (BVMD). The results suggest that this mutation may function as a gain-of-function mutation and disrupt the neck region of the BEST1 channel. These findings not only have implications for understanding the mutations in BEST1, but also contribute to improved diagnosis and prognosis of the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Andrew W. Taylor, Samuel Hsu, Tat Fong Ng
Summary: The retinal pigment epithelial cells (RPE) in the ocular tissue microenvironment play a crucial role in regulating immune cells by expressing immunomodulating molecules to suppress inflammation and promote immune tolerance. Understanding how RPE mediate this regulation and the changes seen under pathological conditions is important in the study of ocular immune privilege.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Chun Austin Changou, Reni Ajoy, Szu-Yi Chou
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2017)
Article
Oncology
J-Y Chuang, W-L Lo, C-Y Ko, S-Y Chou, R-M Chen, K-Y Chang, J-J Hung, W-C Su, W-C Chang, T-I Hsu
Article
Food Science & Technology
Shan-Jen Lin, Yu-Tang Chin, Yih Ho, Szu-Yi Chou, Yu-Chen Sh Yang, Andre Wendindonde Nana, Kuan-Wei Su, Yee-Tong Lim, Kuan Wang, Sheng-Yang Lee, Ya-Jung Shih, Yi-Ru Chen, Jacqueline Whang-Peng, Paul J. Davis, Hung-Yun Lin, Earl Fu
FOOD AND CHEMICAL TOXICOLOGY
(2018)
Article
Food Science & Technology
Yih Ho, Yu-Chen S. H. Yang, Yu-Tang Chin, Szu-Yi Chou, Yi-Ru Chen, Ya-Jung Shih, Jacqueline Whang-Peng, Chun A. Changou, Hsuan-Liang Liu, Shwu-Jiuan Lin, Heng-Yuan Tang, Hung-Yun Lin, Paul J. Davis
FOOD AND CHEMICAL TOXICOLOGY
(2018)
Article
Neurosciences
Chih-Ju Chang, Ming-Yuan Chang, Szu-Yi Chou, Chi-Chen Huang, Jian-Ying Chuang, Tsung-I Hsu, Hsing-Fang Chang, Yi-Hsin Wu, Chung-Che Wu, Daniel Morales, Artur Kania, Tzu-Jen Kao
JOURNAL OF NEUROSCIENCE
(2018)
Article
Oncology
Ching-Yu Lin, Shun-Tai Yang, Shing-Chuan Shen, Yi-Chen Hsieh, Fei-Ting Hsu, Cheng-Yu Chen, Yung-Hsiao Chiang, Jian-Ying Chuang, Kai-Yun Chen, Tsung-I Hsu, Wan-Chong Leong, Yu-Kai Su, Wei-Lun Lo, Yi-Shian Yeh, Yudha Nur Patria, Hsiu-Ming Shih, Che-Chang Chang, Szu-Yi Chou
MOLECULAR ONCOLOGY
(2018)
Article
Multidisciplinary Sciences
Reni Ajoy, Szu-Yi Chou
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2018)
Article
Plant Sciences
Chu-Jen Kuo, Chi-Chen Huang, Szu-Yi Chou, Yu-Chun Lo, Tzu-Jen Kao, Nai-Kuei Huang, Connie Lin, Hsin-Chuan Lin, Hui-Ching Lin, Yi-Chao Lee
Article
Biochemistry & Molecular Biology
Chung-Che Wu, Pin-Tse Lee, Tzu-Jen Kao, Szu-Yi Chou, Ruei-Yuan Su, Yi-Chao Lee, Shiu-Hwa Yeh, Jing-Ping Liou, Tsung-I Hsu, Tsung-Ping Su, Cheng-Keng Chuang, Wen-Chang Chang, Jian-Ying Chuang
Article
Clinical Neurology
N-F Chi, H-Y Chiou, S-Y Chou, C-J Hu, K-Y Chen, C-F Chang, Y-C Hsieh
EUROPEAN JOURNAL OF NEUROLOGY
(2020)
Article
Cell Biology
Szu-Yi Chou, Lung Chan, Chen-Chih Chung, Jing-Yuan Chiu, Yi-Chen Hsieh, Chien-Tai Hong
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Reni Ajoy, Yu-Chun Lo, Man-Hau Ho, You-Yin Chen, Yun Wang, Yuan-Hao Chen, Chiu Jing-Yuan, Chun Austin Changou, Yuan-Chin Hsiung, Hui-Min Chen, Tzu-Hao Chang, Cheng-Yang Lee, Yung-Hsiao Chiang, Wen-Chang Chang, Barry Hoffer, Szu-Yi Chou
Summary: In addition to its neuroinflammatory functions, chemokine CCL5 also exhibits strong neurotrophic properties. CCL5 plays a critical role in promoting hippocampal glucoregulatory efficiency, which in turn enhances synaptic plasticity and memory and cognition functions. CCL5's actions on glucose aerobic metabolism are essential for mitochondrial function, contributing to hippocampal spine and synapse formation, ultimately improving learning and memory.
MOLECULAR PSYCHIATRY
(2021)
Article
Clinical Neurology
Ouada Nebie, Kevin Carvalho, Lassina Barro, Liling Delila, Emilie Faivre, Ting-Yi Renn, Ming-Li Chou, Yu-Wen Wu, Ariunjargal Nyam-Erdene, Szu-Yi Chou, Luc Buee, Chaur-Jong Hu, Chih-Wei Peng, David Devos, David Blum, Thierry Burnouf
Summary: TBI causes significant brain anatomopathological damages which can be addressed by a synergistic biotherapy such as human platelet lysate. Its therapeutic potential was tested in in vitro and in vivo TBI models, showing promising results in improving motor function and mitigating neuroinflammation and oxidative stress. The data strongly support that human platelet lysate is a reliable and effective source of neurorestorative factors for brain trauma treatment.
Article
Biochemistry & Molecular Biology
Man-Hau Ho, Chia-Hung Yen, Tsung-Hsun Hsieh, Tzu-Jen Kao, Jing-Yuan Chiu, Yung-Hsiao Chiang, Barry J. Hoffer, Wen-Chang Chang, Szu-Yi Chou
Summary: This study found that CCL5 plays an important role in activating GPX1 antioxidant activity during days 1-3 post-injury, protecting hippocampal neurons from ROS damage, and improving memory function after trauma.
Article
Anatomy & Morphology
V. Chia-Hsuan Tseng, Chee Ho Chew, Wan-Ting Huang, Yang-Kao Wang, Ko-Shao Chen, Szu-Yi Chou, Chien-Chung Chen
CELLS TISSUES ORGANS
(2017)