Review
Microbiology
Anusha Naganathan, Gloria M. Culver
Summary: The production of ribosomal subunits directly affects the fitness of bacteria, and extra-ribosomal protein factors play a crucial role in this process.
ANNUAL REVIEW OF MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Kerstin Doerner, Chiara Ruggeri, Ivo Zemp, Ulrike Kutay
Summary: The assembly of ribosomal subunits is a highly coordinated process involving many accessory factors. Most of these factors have been identified through genetic screening and proteomic approaches in yeast, and their requirement is conserved in evolution. However, the function and mechanism of action of many factors remained unknown until recently, when structural, biochemical, and cellular studies provided a detailed understanding of their role in ribosome maturation.
Article
Biochemistry & Molecular Biology
Cristina Remes, Anas Khawaja, Sarah F. Pearce, Adam M. Dinan, Shreekara Gopalakrishna, Miriam Cipullo, Vasileios Kyriakidis, Jingdian Zhang, Xaquin Castro Dopico, Olessya Yukhnovets, Ilian Atanassov, Andrew E. Firth, Barry Cooperman, Joanna Rorbach
Summary: The detailed mechanism of mitochondrial translation in human mitochondria remains unclear. This study reveals that leaderless mRNA transcripts can be directly loaded onto assembled 55S mitochondrial ribosomes in human mitochondria, but not onto the small subunit, and this process requires initiator fMet-tRNA(Met) binding. Furthermore, this study demonstrates that mtIF3 is not involved in translation of leaderless mitochondrial transcripts but is essential for translation of ATP6 in the bicistronic ATP8/ATP6 transcript. These findings provide insights into the evolutionary divergence of mitochondrial translation and enhance our understanding of a critical process in eukaryotic metabolism.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Yuxin Wang, Yixun Su, Guangdan Yu, Xiaorui Wang, Xiaoying Chen, Bin Yu, Yijun Cheng, Rui Li, Juan C. Saez, Chenju Yi, Lan Xiao, Jianqin Niu
Summary: This study demonstrates that reduced oligodendrocyte precursor cells (OPCs) are associated with hindered hippocampal astrocytic development in an improved parental isolation mouse model, and loss of OPCs suppresses astrocytic network formation and activity. Moreover, OPC-derived Wnt ligands, particularly Wnt7b, are essential for Wnt/beta-catenin pathway-mediated astrocytic development and neuronal function. Local replenishment of Wnt7a/b is sufficient to rescue astrocytic maldevelopment.
Article
Biochemistry & Molecular Biology
Ingrid Roessler, Sarah Weigl, Jose Fernandez-Fernandez, Sara Martin-Villanueva, Daniela Strauss, Ed Hurt, Jesus de la Cruz, Brigitte Pertschy
Summary: The small ribosomal subunit protein Rps15/uS19 is involved in both nucleolar ribosome assembly and cytoplasmic pre-40S maturation. The C-terminal tail of Rps15 plays a role in quality control during late pre-40S maturation, ensuring functional Rps15 C-terminal tail in 40S ribosomal subunits entering translation.
Article
Multidisciplinary Sciences
Jingdong Cheng, Otto Berninghausen, Roland Beckmann
Summary: Assembly of the mitoribosome involves numerous assembly factors, with late transitions mainly involving rRNA domains IV and V. Structures of the human 39S late assembly intermediates reveal several assembly factors maintaining the immature conformations of 16S rRNA, and unexpected deacylated tRNA in the ribosomal E-site, suggesting a role in 39S assembly.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Emma S. V. Andrews, Wayne M. Patrick
Summary: Ribosomes are protein production machines in cells, and the process of ribosome biogenesis is not fully understood. YbeY and YbeZ are two genes involved in RNA-related functions, and they participate together in the maturation of 16S rRNA and protein synthesis. A model is proposed to explain the function of YbeZ in 16S rRNA maturation.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Gaurav Verma, Alexander Bowen, Sevda Gheibi, Alexander Hamilton, Sowndarya Muthukumar, Luis Rodrigo Cataldo, Olof Asplund, Jonathan Esguerra, Alexandros Karagiannopoulos, Claire Lyons, Elaine Cowan, Cristian Bellodi, Rashmi Prasad, Malin Fex, Hindrik Mulder
Summary: DIMT1 deficiency leads to mitochondrial dysfunction and disrupted insulin secretion, which may be one of the pathogenic processes in type 2 diabetes (T2D).
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Oncology
Derek B. Oien, Upasana Ray, Christopher L. Pathoulas, Ling Jin, Prabhu Thirusangu, Deokbeom Jung, Joseph E. Kumka, Yinan Xiao, Sayantani Sarkar Bhattacharya, Dennis Montoya, Jeremy Chien, Viji Shridhar
Summary: This study investigates the mechanism of action of quinacrine in chemoresistant ovarian cancer cells, finding that it increases DNA damage by inhibiting the ribosomal biogenesis pathway and inducing nucleolar stress, ultimately sensitizing cells to PARP inhibitors. This supports new therapeutic strategies for treating refractory ovarian cancer.
Article
Microbiology
Xinxing Yang, Ryan McQuillen, Zhixin Lyu, Polly Phillips-Mason, Ana de la Cruz, Joshua W. McCausland, Hai Liang, Kristen E. DeMeester, Cintia C. Santiago, Catherine L. Grimes, Piet de Boer, Jie Xiao
Summary: The study identified two populations of FtsW molecules during bacterial cell division: a fast-moving group associated with FtsZ dynamics and a slow-moving group correlated with active sPG synthesis. FtsN was also found to play a role in promoting the slow-moving population, suggesting a two-track model for the spatial and temporal coordination of sPG synthesis.
NATURE MICROBIOLOGY
(2021)
Article
Pediatrics
Xia Zhang, Cun Liu, Yi Cao, Li Liu, Fusheng Sun, Lin Hou
Summary: This study found that RRS1 plays a role in regulating proliferation and cell cycle in neuroblastoma, and knockdown of RRS1 can inhibit cell proliferation through the PI3K/Akt/NF-kappa B pathway. Therefore, RRS1 may be a potential target for neuroblastoma treatment.
PEDIATRIC RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jingdong Cheng, Benjamin Lau, Matthias Thoms, Michael Ameismeier, Otto Berninghausen, Ed Hurt, Roland Beckmann
Summary: This study reveals the process of biogenesis of the small ribosomal subunit in eukaryotes and describes the structural transitions of assembly intermediates in detail. These findings demonstrate the evolutionary conserved mechanism of yeast and human assembly of the 40S ribosomal subunit, as well as some minor differences.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Medicine, Research & Experimental
Matteo Rossi, Mikhail Steklov, Fanny Huberty, Thuy Nguyen, Jerome Marijsse, Celine Jacques-Hespel, Paul Najm, Caroline Lonez, Eytan Breman
Summary: Genome engineering technologies are powerful tools for optimizing cell functionality. A microRNA-based multiplex shRNA platform can deliver multiple shRNA-like sequences, allowing for precise modulation of target genes.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Biochemistry & Molecular Biology
Mason A. McCool, Amber F. Buhagiar, Carson J. Bryant, Lisa M. Ogawa, Laura Abriola, Yulia V. Surovtseva, Susan J. Baserga
Summary: This study shows that the nucleolar protein RSL24D1 and the PeBoW complex play important roles in multiple steps of ribosome biogenesis, including pre-ribosomal RNA transcription and mature 28S rRNA production. They regulate pre-ribosomal RNA transcription by modulating the steady-state levels of RNA polymerase I. Specifically, RSL24D1 is demonstrated to coimmunoprecipitate with the RNA polymerase I subunit, RPA194, and together regulate the process of ribosome biogenesis.
Article
Multidisciplinary Sciences
Yazhong Cui, Yang Miao, Longzhi Cao, Lifang Guo, Yue Cui, Chuanzhe Yan, Zhi Zeng, Mo Xu, Ting Han
Summary: The loss of melanocortin-1 receptor (MC1R) in melanoma cells is associated with enhanced T cell infiltration and anti-tumor immune responses.
NATURE COMMUNICATIONS
(2023)