期刊
MOLECULAR BIOLOGY OF THE CELL
卷 20, 期 2, 页码 658-672出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E08-05-0482
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资金
- National Institutes of Health [AI-060767]
- American Heart Association [0230079N, 0755368U]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI060767] Funding Source: NIH RePORTER
The inability to synthesize cholesterol is universal among protozoa. The intracellular pathogen Toxoplasma depends on host lipoprotein-derived cholesterol to replicate in mammalian cells. Mechanisms of cholesterol trafficking in this parasite must be important for delivery to proper organelles. We characterized a unique D-bifunctional protein variant expressed by Toxoplasma consisting of one N-terminal D-3-hydroxyacyl-CoA dehydrogenase domain fused to two tandem sterol carrier protein-2 (SCP-2) domains. This multidomain protein undergoes multiple cleavage steps to release free SCP-2. The most C-terminal SCP-2 carries a PTS1 that directs the protein to vesicles before processing. Abrogation of this signal results in SCP-2 accumulation in the cytoplasm. Cholesterol specifically binds to parasite SCP-2 but with 10-fold lower affinity than phosphatidylcholine. In mammalian cells and Toxoplasma, the two parasite SCP-2 domains promote the circulation of various lipids between organelles and to the surface. Compared with wild-type parasites, TgHAD-2SCP-2-transfected parasites replicate faster and show enhanced uptake of cholesterol and oleate, which are incorporated into neutral lipids that accumulate at the basal end of Toxoplasma. This work provides the first evidence that the lipid transfer capability of an ancestral eukaryotic SCP-2 domain can influence the lipid metabolism of an intracellular pathogen to promote its multiplication in mammalian cells.
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