Article
Biochemistry & Molecular Biology
Andras Zeke, Laszlo Dobson, Levente Istvan Szekeres, Tamas Lango, Gabor E. Tusnady
Summary: The article introduces a comprehensive database containing experimentally verified mammalian proteins displaying polarized sorting or secretion, with a focus on epithelial cells. This database provides various information relevant to cell secretion and sorting, which can be valuable for researchers in conducting related studies or comparative analyses.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Satoshi Hirano, Ryohei Goto, Yasuo Uchida
Summary: This study developed a comprehensive method to determine the localization of apical and basolateral membrane proteins, and applied it to analyze mouse liver proteome. The method successfully distinguished different membrane-localized proteins and revealed the differential protein expression between apical and basolateral membranes.
Article
Biochemical Research Methods
Laszlo Dobson, Andras Zeke, Gabor E. Tusnady
Summary: This study utilized neural networks to classify transmembrane proteins on apical and basolateral membranes, aiding in understanding drug transport and its effects. The method provided may assist researchers in identifying or characterizing molecular networks regulating the distribution of transporters or receptors.
Article
Cell Biology
Alexandra A. M. Fischer, Larissa Schatz, Julia Baaske, Winfried Roemer, Wilfried Weber, Roland Thuenauer
Summary: Each cell in a multicellular organism adjusts the concentration of its cell surface proteins, and a novel approach based on split luciferases allows for sensitive measurement of protein concentration in live cells. The method uses split luciferase fragments that generate luminescence when the protein of interest arrives at the cell surface.
Article
Biology
Qingqing Zhao, Yang Kong, Alec Kittredge, Yao Li, Yin Shen, Yu Zhang, Stephen H. Tsang, Tingting Yang
Summary: This study reveals that loss-of-function and gain-of-function mutations of the BEST1 gene in patients exhibit different requirements for mutant to wild-type molecule ratios, suggesting distinct epigenetic requirements in bestrophinopathy development. Furthermore, gain-of-function mutations have a strong dominant effect that impedes the restoration of BEST1-dependent Cl- currents in RPE cells by gene augmentation, unlike the rescue of loss-of-function mutations. It is also shown that gain-of-function mutations can be rescued by a combination of gene augmentation and CRISPR/Cas9-mediated knockdown of endogenous BEST1 expression, providing a universal treatment strategy for bestrophinopathy patients with different mutation types.
Article
Immunology
Zhirong Zhang, Rossella Venditti, Li Ran, Zengzhen Liu, Karl Vivot, Annette Schurmann, Juan S. Bonifacino, Maria Antonietta De Matteis, Romeo Ricci
Summary: The NLRP3 inflammasome plays a crucial role in the innate immune response, and disruption of the endoplasmic reticulum-endosome membrane contact sites (EECS) leads to NLRP3 accumulation in endosomes and activation of the inflammasome. Lowering endosomal phosphatidylinositol 4-phosphate (PI4P) levels prevents NLRP3 association with endosomes and inhibits inflammasome activation. This discovery helps us better understand the activation mechanism of the inflammasome.
Article
Biochemistry & Molecular Biology
Anika Koetemann, Bernd Wollscheid
Summary: This article discusses the importance of apicobasal polarity and surfaceomes in epithelial cells, and identifies potential key regulators of these processes. By using chemoproteomic surfaceome scanning, proteotype maps were established and quantitative distributions of proteins that affect polarized cell functionality were revealed. The study also found that the tumor suppressor PTEN regulates the architecture of the polarized surfaceome and may play a role in collective cell migration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Hannah Faris, Mohammadali Almasieh, Leonard A. Levin
Summary: PS and PE exhibit distinct patterns of externalization in normal and degenerating neurons, suggesting a differential role for these phospholipids in transducing neuronal injury.
CELL DEATH DISCOVERY
(2021)
Article
Multidisciplinary Sciences
Elisabetta Mantuano, Pardis Azmoon, Michael A. Banki, Cory B. Gunner, Steven L. Gonias
Summary: In this study, we compared three ligands of LRP1 that inhibit inflammatory responses triggered by LPS. These ligands activate cell signaling and require N-methyl-D-aspartate receptor and intact lipid rafts. Among them, alpha M-2 is the only ligand that absolutely requires LRP1, while S-PrP can activate cell signaling independently of membrane-anchored PrPc.
SCIENTIFIC REPORTS
(2022)