Article
Biochemistry & Molecular Biology
Yaqing Huang, Yanmei Zheng, Qianqian Wang, Caixia Qi
Summary: This study evaluated the role of Rolipram in the migration and invasion of human choriocarcinoma cells. The results showed that Rolipram could suppress the migration and invasion of choriocarcinoma cells by inhibiting PDE4, and this was accompanied by decreased expression of MMP9 and TIMP1, as well as increased expression of E-cadherin and decreased expression of vimentin.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2023)
Article
Multidisciplinary Sciences
Phuong Thao Ly, Shaohai Xu, Melissa Wirawan, Dahai Luo, Xavier Roca
Summary: The study demonstrates that different isoforms of ZAP play overlapping yet different roles in antiviral defense and other cellular processes. The regulation of host mRNA by ZAP is mostly unknown, but splice-switching antisense oligonucleotides can modulate the balance between ZAP isoforms. The study also identifies new cellular transcripts and processes regulated by ZAP isoforms, such as unfolded protein response, epithelial-mesenchymal transition, and innate immunity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Nguyen-Tuong Ho, Shu-Wei Lin, Yi-Rong Lee, Chii-Ruey Tzeng, Shu-Huei Kao
Summary: OPNb and OPNc are overexpressed in endometriosis and adenomyosis, and CD44v and the epithelial-mesenchymal transition (EMT) process are also upregulated. Overexpression of OPNb and OPNc splice variants leads to morphological changes, actin remodeling, cell proliferation, cell migration, and EMT through binding with CD44 and alpha v beta 3, subsequently activating the PI3K and NF-kappa B pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Haobin Jiang, Lina Bai, Shen Song, Qianqian Yin, Anteng Shi, Bin Zhou, Hong Lian, Houzao Chen, Cheng-Ran Xu, Yanchun Wang, Yu Nie, Shengshou Hu
Summary: In this study, the role of EZH2 in epicardial development was investigated. The results demonstrate that EZH2 promotes epicardial cell migration by suppressing TIMP3 transcription, which is vital for heart development. Moreover, epicardial Ezh2 deficiency-induced TIMP3 upregulation leads to extracellular matrix reconstruction in the embryonic myocardium.
LIFE SCIENCE ALLIANCE
(2023)
Article
Multidisciplinary Sciences
Rong Li, Xiaoqiu Wang, Zhenyao Huang, Jayani Balaji, Tae Hoon Kim, Tianyuan Wang, Lecong Zhou, Ashley Deleon, Molly E. Cook, Margeaux W. Marbrey, San-Pin Wu, Jae Wook Jeong, Ripla Arora, Francesco J. DeMayo
Summary: The loss of uterine epithelial progesterone receptor (PGR) is crucial for embryo implantation in both humans and mice. This study reveals that the two major isoforms PGRA and PGRB disrupt embryo implantation through FOXO1 pathways in uterine epithelium, and both regulate the LIF-SGK1-FOXO1 signaling pathway.
Article
Cell Biology
Katharina Scheibner, Silvia Schirge, Ingo Burtscher, Maren Buettner, Michael Sterr, Dapeng Yang, Anika Boettcher, Ansarullah, Martin Irmler, Johannes Beckers, Filippo M. Cernilogar, Gunnar Schotta, Fabian J. Theis, Heiko Lickert
Summary: The mesoderm is formed by classical EMT, while the endoderm is formed independently of Snail1 through epithelial cell plasticity mechanisms. Foxa2 acts as an epithelial gatekeeper and EMT suppressor, protecting the endoderm from undergoing a mesenchymal transition.
NATURE CELL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Rosalia Gagliardo, Fabio Bucchieri, Angela Marina Montalbano, Giusy Daniela Albano, Delphine Gras, Alberto Fucarino, Roberto Marchese, Giulia Anzalone, Chiara Lo Nigro, Pascal Chanez, Mirella Profita
Summary: The airway epithelium plays a critical role in the pathophysiology of COPD, with dysregulation of Oct4, CD146, and CD105 expression induced by cigarette smoke exposure potentially exacerbating airway epithelial dysfunction and contributing to disease progression.
Article
Dentistry, Oral Surgery & Medicine
Yuri Miyakawa, Risako Chiba-Ohkuma, Takeo Karakida, Ryuji Yamamoto, Saeko Kobayashi, Yasuo Yamakoshi, Yoshinobu Asada
Summary: This study investigated the differential response of TGF beta isoforms to epithelial-mesenchymal transition (EMT) in enamel epithelial cells. The results showed that EMT occurred in the presence of TGF-beta 1 and beta 3, but not TGF-beta 2. The Rho signaling pathway was identified as the main pathway associated with TGF-beta-induced EMT. The differences in response across TGF-beta isoforms were attributed to their endoglin-mediated binding affinity for the TGF-beta receptor.
ARCHIVES OF ORAL BIOLOGY
(2022)
Review
Oncology
Debra R. Garvey, Gagan Chhabra, Mary A. Ndiaye, Nihal Ahmad
Summary: PLK4, a member of the polo-like kinase family, plays a role in regulating centriole duplication during the cell cycle, and is associated with overexpression in various human cancers, suggesting a potential role in poor cancer prognosis. Inhibition of PLK4 has shown promising results in inducing cancer cell death, but the underlying mechanisms are still largely unknown.
MOLECULAR CANCER THERAPEUTICS
(2021)
Review
Oncology
Cosima C. Hoch, Fabian Stoegbauer, Barbara Wollenberg
Summary: Salivary adenoid cystic carcinoma (SACC) is an aggressive tumor with a high tendency for metastasis. Epithelial-mesenchymal transition (EMT) plays a crucial role in the invasiveness of SACC by enhancing mobility and invasiveness. This manuscript reviews the latest research on potential biomarkers of EMT in SACC, aiming to identify new therapeutic targets and improve patient management.
Article
Medicine, General & Internal
Eabha O'Driscoll, Emily Hughes, Mustapha Irnaten, Markus Kuehn, Deborah Wallace, Colm O'Brien
Summary: Optic nerve head cupping, a feature of glaucoma, is associated with extracellular matrix remodeling and lamina cribrosa fibrosis. Peripapillary atrophy is common in glaucoma and involves the loss of retinal pigment epithelium adjacent to the optic nerve head. Under pro-fibrotic conditions, epithelial cells can undergo epithelial-to-mesenchymal transition and contribute to extracellular matrix fibrosis. This study investigates the role of this process in glaucoma and peripapillary atrophy, demonstrating the presence of retinal pigment epithelium cells in glaucomatous optic nerve heads and the potential of these cells to differentiate into fibroblast-like cells.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Oncology
Yingying Luo, Danfang Deng, Lamei Lin, Yikun Zhou, Lan Wang, Xinrong Zou, Xiaoqin Wang
Summary: This study found that different isoforms of FGF2 played different roles in EMT in diabetic nephropathy, with LMW FGF2 promoting the process and HMW FGF2 exerting a protective effect.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Genetics & Heredity
Xiangtian Yu, XiaoYong Pan, ShiQi Zhang, Yu-Hang Zhang, Lei Chen, Sibao Wan, Tao Huang, Yu-Dong Cai
Summary: This study established a systematic workflow integrating effective feature selection, multiple machine learning models (Random forest RF, Support vector machine SVM), rule learning, and functional enrichment analyses to find new biomarkers for distinguishing single-cell isolated TCs with unique epithelial or mesenchymal markers. The discovered signatures may provide an effective and precise transcriptomic reference to monitor EMT progression at the single-cell level and contribute to the exploration of detailed tumorigenesis mechanisms during EMT.
FRONTIERS IN GENETICS
(2021)
Review
Oncology
Mark Primeaux, Saiprasad Gowrikumar, Punita Dhawan
Summary: Cellular plasticity is crucial for cancer progression, metastasis, and treatment resistance. The concepts of stemness and epithelial-mesenchymal plasticity in cancer reflect the cancer cells' ability to adopt and adapt developmental programs to enhance survival and growth. CD44, a cell adhesion molecule, is not only a widely accepted marker for cancer stem cells, but also a key player in regulating both stemness and epithelial-mesenchymal plasticity. Understanding the dynamics and effects of CD44 isoform expressions in cancer can provide valuable insights for future research and therapeutic interventions.
CLINICAL & EXPERIMENTAL METASTASIS
(2022)
Article
Cell Biology
Siqin Yang, Hwajung Choi, Tak-Heun Kim, Ju-Kyung Jeong, Yudong Liu, Hidemitsu Harada, Eui-Sic Cho
Summary: Fine control of beta-catenin is crucial for Hertwig's epithelial root sheath (HERS) integrity and junctional protein expression, which play pivotal roles in tooth root formation. Stabilization of beta-catenin promotes unfragmented HERS formation and prevents HERS dissociation, while its destabilization leads to premature disruption of HERS and interrupted root elongation.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Critical Care Medicine
Roxana Wasnick, Martina Korfei, Katarzyna Piskulak, Ingrid Henneke, Jochen Wilhelm, Poornima Mahavadi, Ruth Charlotte Dartsch, Daniel von der Beck, Miriam Koch, Irina Shalashova, Astrid Weiss, Oleksiy Klymenko, Ingolf Askevold, Ludger Fink, Heiko Witt, Holger Hackstein, Elie El Agha, Saverio Bellusci, Walter Klepetko, Melanie Koenigshoff, Oliver Eickelberg, Ralph Theo Schermuly, Thomas Braun, Werner Seeger, Clemens Ruppert, Andreas Guenther
Summary: This study reveals that Notch signaling plays a crucial role in the proliferation and dedifferentiation of type II alveolar epithelial cells (AEC2s), as well as the development of lung fibrosis in idiopathic pulmonary fibrosis (IPF).
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Vitalii Kryvenko, Andres Alberro-Brage, Athanasios Fysikopoulos, Miriam Wessendorf, Khodr Tello, Rory E. Morty, Susanne Herold, Werner Seeger, Christos Samakovlis, Istvan Vadasz
Summary: A study investigated the mechanisms of cellular albumin uptake in a three-dimensional culture of precision-cut lung slices (PCLS). The study found that PCLS cells incorporate labeled albumin in a time- and concentration-dependent manner and identified a receptor-driven internalization process for albumin uptake. The findings highlight the potential for improving outcomes in patients with respiratory failure through pulmonary drug delivery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cardiac & Cardiovascular Systems
Manuel J. Richter, Federico Fortuni, Fawaz Alenezi, Michele D'Alto, Roberto Badagliacca, Nathan W. Brunner, Arie P. van Dijk, Philipp Douschan, Henning Gall, Stefano Ghio, Francesco Lo Giudice, Ekkehard Grunig, Francois Haddad, Luke Howard, Sudarshan Rajagopal, Niels Stens, Davide Stolfo, Dick H. J. Thijssen, Carmine Dario Vizza, Roham T. Zamanian, Liang Zhong, Werner Seeger, Hossein A. Ghofrani, Khodr Tello
Summary: A meta-analysis of 35 studies found that right atrial (RA) imaging assessment is a relevant prognostic marker in patients with pulmonary hypertension (PH). RA area, RA indexed area, RA peak longitudinal strain, and RA total emptying fraction were significantly associated with combined end-points including death, clinical worsening, and/or lung transplantation. RA volume and volume index showed marginal significant associations.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Article
Medicine, General & Internal
William G. Herrington, Natalie Staplin, Christoph Wanner, Jennifer B. Green, Sibylle J. Hauske, Jonathan R. Emberson, David Preiss, Parminder Judge, Kaitlin J. Mayne, Sarah Y. A. Ng, Emily Sammons, Doreen Zhu, Michael Hill, Will Stevens, Karl Wallendszus, Susanne Brenner, Alfred K. Cheung, Zhi-Hong Liu, Jing Li, Lai Seong Hooi, Wen Liu, Takashi Kadowaki, Masaomi Nangaku, Adeera Levin, David Cherney, Aldo P. Maggioni, Roberto Pontremoli, Rajat Deo, Shinya Goto, Xavier Rossello, Katherine R. Tuttle, Dominik Steubl, Michaela Petrini, Dan Massey, Jens Eilbracht, Martina Brueckmann, Martin J. Landray, Colin Baigent, Richard Haynes
Summary: Empagliflozin therapy reduces the risk of disease progression or cardiovascular death in patients with chronic kidney disease.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Medicine, General & Internal
Fritz C. Roller, Armin Schuessler, Nils Kremer, Sebastian Harth, Steffen D. Kriechbaum, Christoph B. Wiedenroth, Stefan Guth, Andreas Breithecker, Manuel Richter, Khodr Tello, Werner Seeger, Eckhard Mayer, Gabriele A. Krombach
Summary: The aim of this study was to assess the RV and LV blood pool T1 and T2 values in CTEPH patients and their correlation to pulmonary hemodynamic. Results showed that compared to control subjects, CTEPH patients had significantly reduced RV T2 values, which increased significantly after balloon pulmonary angioplasty. RV T2 values were positively correlated with hemodynamic measures. Therefore, RV T2 mapping can be a novel non-invasive CMR imaging marker for assessing disease severity, prognosis, follow-up, and therapy monitoring in PH.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Cardiac & Cardiovascular Systems
Baktybek Kojonazarov, Norbert Weissmann, Werner Seeger, Ralph Theo Schermuly
CARDIOLOGY JOURNAL
(2023)
Article
Respiratory System
Rogerio Souza, David B. Badesch, H. Ardeschir Ghofrani, J. Simon R. Gibbs, Mardi Gomberg-Maitland, Vallerie V. Mclaughlin, Ioana R. Preston, Aaron B. Waxman, Ekkehard Gruenig, Grzegorz Kope, Gisela Meyer, Karen M. Olsson, Stephan Rosenkranz, Jianxin Lin, Amy O. Johnson-Levonas, Janethe de Oliveira Pena, Marc Humbert, Marius M. Hoeper
Summary: Sotatercept demonstrated significant improvements in pulmonary artery pressure and right heart function in pre-treated patients with pulmonary arterial hypertension (PAH).
EUROPEAN RESPIRATORY JOURNAL
(2023)
Article
Cardiac & Cardiovascular Systems
Zvonimir A. Rako, Athiththan Yogeswaran, Balint K. Lakatos, Alexandra Fabian, Selin Yildiz, Bruno Brito da Rocha, Istvan Vadasz, Hossein Ardeschir Ghofrani, Werner Seeger, Henning Gall, Nils C. Kremer, Manuel J. Richter, Pascal Bauer, Ryan J. Tedford, Robert Naeije, Attila Kovacs, Khodr Tello
Summary: This study aimed to assess the relationship between right ventricular contraction pattern and right ventricular-pulmonary arterial (RV-PA) coupling. The results showed that early RV-PA uncoupling is associated with reduced longitudinal function, while advanced uncoupling leads to reduced anteroposterior movement and left ventricular preload. These findings are important for risk assessment in patients.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Article
Immunology
Andres Alberro-Brage, Vitalii Kryvenko, Christina Malainou, Stefan Guenther, Rory E. Morty, Werner Seeger, Susanne Herold, Christos Samakovlis, Istvan Vadasz
Summary: Acute respiratory distress syndrome (ARDS) is a common complication of influenza virus (IV) infection, which leads to the accumulation and impairment of alveolar protein. It has been found that IV infection inhibits the clearance of alveolar protein, possibly through upregulation of a proteinase called MMP. In addition, inhibition of MMP proteinase may improve the clearance of alveolar protein and thus provide a potential therapeutic approach for ARDS.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, General & Internal
Dirk Bandorski, Khodr Tello, Harun Erdal, Janine Sommerlad, Jochen Wilhelm, Istvan Vadasz, Matthias Hecker, Dieter Walmrath, Werner Seeger, Ekaterina Krauss, Stefan Kuhnert
Summary: This study evaluates the diagnostic value of gastric biomarkers, such as pepsin and bile acid, in detecting aspiration in the intensive care unit (ICU). The results indicate that measuring pepsin and bile acid does not reliably diagnose aspiration or significantly impact survival outcomes. Further validation studies are needed to assess the clinical usefulness and reliability of these biomarkers in diagnosing aspiration-related conditions.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Respiratory System
Athiththan Yogeswaran, Zvonimir A. Rako, Selin Yildiz, Hossein Ardeschir Ghofrani, Werner Seeger, Bruno Brito da Rocha, Henning Gall, Nils C. Kremer, Philipp Douschan, Silvia Papa, Carmine Dario Vizza, Domenico Filomena, Ryan J. Tedford, Robert Naeije, Manuel J. Richter, Roberto Badagliacca, Khodr Tello
Summary: Our study demonstrates the usefulness of echocardiography-derived S'/RAAi for noninvasive assessment of RV diastolic function and prognosis in PH.
Review
Biochemistry & Molecular Biology
Soni Savai Pullamsetti, Ravikumar Sitapara, Robin Osterhout, Astrid Weiss, Laura L. Carter, Lawrence S. Zisman, Ralph Theo Schermuly
Summary: Pulmonary arterial hypertension (PAH) is characterized by vascular remodeling and increased pulmonary vascular resistance. Histologically, PAH is characterized by plexiform and neointimal lesions composed of dysregulated endothelial cells and myofibroblasts. The PDGFR-CSF1R-c-KIT signaling network has been implicated in PAH pathogenesis, and inhibition of this network has potential therapeutic benefits. Seralutinib, which targets these pathways, shows promise for the treatment of PAH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biophysics
Milad Eyvazi Hesar, Niloofar Sadat Seyedsadrkhani, Dibyendu Khan, Adib Naghashian, Mateusz Piekarski, Henning Gall, Ralph Schermuly, Hossein Ardeschir Ghofrani, Sven Ingebrandt
Summary: This research presents a wearable epidermal electronic system for continuous monitoring of a prognostic parameter for hypertension. The system is flexible, wireless, and smartphone-enabled, allowing real-time monitoring of electrocardiograms and seismocardiograms. The system shows promising performance in predicting hypertension.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Cardiac & Cardiovascular Systems
Marc Humbert, Gabor Kovacs, Marius M. Hoeper, Roberto Badagliacca, Rolf M. F. Berger, Margarita Brida, Jorn Carlsen, Andrew J. S. Coats, Pilar Escribano-Subias, Pisana Ferrari, Diogenes S. Ferreira, Hossein Ardeschir Ghofrani, George Giannakoulas, David G. Kiely, Eckhard Mayer, Gergely Meszaros, Blin Nagavci, Karen M. Olsson, Joanna Pepke-Zaba, Jennifer K. Quint, Goran Radegran, Gerald Simonneau, Olivier Sitbon, Thomy Tonia, Mark Toshner, Jean-Luc Vachiery, Anton Vonk Noordegraaf, Marion Delcroix, Stephan Rosenkranz, Markus Schwerzmann, Anh-Tuan Dinh-Xuan, Andy Bush, Magdy Abdelhamid, Victor Aboyans, Eloisa Arbustini, Riccardo Asteggiano, Joan-Albert Barbera, Maurice Beghetti, Jelena Celutkiene, Maja Cikes, Robin Condliffe, Frances de Man, Volkmar Falk, Laurent Fauchier, Sean Gaine, Nazzareno Galie, Wendy Gin-Sing, John Granton, Ekkehard Gruenig, Paul M. Hassoun, Merel Hellemons, Tiny Jaarsma, Barbro Kjellstrom, Frederikus A. Klok, Aleksandra Konradi, Konstantinos C. Koskinas, Dipak Kotecha, Irene Lang, Basil S. Lewis, Ales Linhart, Gregory Y. H. Lip, Maja-Lisa Lochen, Alexander G. Mathioudakis, Richard Mindham, Shahin Moledina, Robert Naeije, Eva Prescott, Amina Rakisheva, Abilio Reis, Arsen D. Ristic, Jens Cosedis Nielsen, Horst Olschewski, Isabelle Opitz, Steffen E. Petersen
GIORNALE ITALIANO DI CARDIOLOGIA
(2023)
Letter
Urology & Nephrology
Hristos Karakizlis, Vipul Agarwal, Mostafa Aly, Kevin Strecker, Benjamin Csala, Isla Esso, Jiangping Chen, Christian Nahrgang, Martin Wolter, Heiko Slanina, Christian G. Schuettler, Soenke Jessen, Claudio Ronco, Werner Seeger, Rolf Weimer, Martina Sester, Horst-Walter Birk, Faeq Husain-Syed
JOURNAL OF NEPHROLOGY
(2023)
