Article
Chemistry, Medicinal
Ahmed Alafnan, Abdulwahab Alamri, Talib Hussain, Syed Mohd Danish Rizvi
Summary: This study elucidates the anticancer efficacy of Cur-B against prostate cancer cells PC3, with findings including reduced cell viability, nuclear fragmentation and condensation, activation of caspase, ROS-mediated oxidative stress, dissipation of mitochondrial membrane potential, downregulation of JAK/STAT signaling cascade, and increased expression of CDK inhibitor p21(Cip1).
Article
Developmental Biology
Sumitra Tatapudy, Jobelle Peralta, Todd Nystul
Summary: In Drosophila ovarian epithelial follicle stem cells, the E2 ubiquitin ligase, Bendless, plays multiple roles in JNK signaling, differentiation, proliferation, and niche competition, with a relationship to hedgehog signaling pathway regulation.
Article
Biochemistry & Molecular Biology
Jiajia Gao, Yan Gao, Guiran Xiao
Summary: This study reveals the crucial role of the zinc transporter Catsup in ovary morphogenesis, as knockdown of Catsup in escort cells leads to defects in niche establishment and germline stem cell self-renewal. Further investigations demonstrate that Catsup affects adult ovary formation by suppressing Notch signaling, and the defects caused by Catsup knockdown can be restored by overexpressing heat shock cognate protein 70 (Hsc70).
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Wenyuan Wang, Ye-Fan Hu, Meijun Pang, Nannan Chang, Chunxiao Yu, Qi Li, Jing-Wei Xiong, Yuanyuan Peng, Ruilin Zhang
Summary: The study demonstrated the crucial roles of BMP and Notch signaling pathways in zebrafish heart regeneration, but the interaction between them and the specific mechanisms in cardiomyocyte proliferation still require further exploration.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Multidisciplinary Sciences
Mehmet Neset ozel, Felix Simon, Shadi Jafari, Isabel Holguera, Yen-Chung Chen, Najate Benhra, Rana Naja El-Danaf, Katarina Kapuralin, Jennifer Amy Malin, Nikolaos Konstantinides, Claude Desplan
Summary: Deciphering neuronal diversity in the Drosophila optic lobe during pupal development reveals transient extrinsic neurons and dorsoventral asymmetry in visual circuits.
Article
Cell Biology
Deeptiman Chatterjee, Fei Cong, Xian-Feng Wang, Caique Almeida Machado Costa, Yi-Chun Huang, Wu -Min Deng
Summary: In proliferating neoplasms, microenvironment-derived selective pressures promote tumor heterogeneity by imparting diverse capacities for growth, differentiation, and invasion. However, what makes a tumor cell respond to signaling cues differently from a normal cell is not well understood.
Article
Cell Biology
Allison Jevitt, Yi-Chun Huang, Su-Mei Zhang, Deeptiman Chatterjee, Xian-Feng Wang, Geng-Qiang Xie, Wu-Min Deng
Summary: Research has shown that the Notch signaling pathway is dysregulated in many cancer types, often through nuclear accumulation of the active receptor NICD. Using a tumor model in Drosophila ovarian follicular epithelium, it was found that NICD accumulation contributes to tumor growth and reduced apoptosis. Key genes involved in the response to NICD accumulation during tumorigenesis were identified through bulk RNA sequencing.
Article
Developmental Biology
Dongyu Jia, Allison Jevitt, Yi-Chun Huang, Belen Ramos, Wu-Min Deng
Summary: Epithelial cell shape transition between cuboidal and squamous shapes in Drosophila egg chamber is regulated by Notch signaling, ecdysone signaling, and JAK/STAT signaling. Ecdysone signaling is essential for cell stretching, while JAK/STAT signaling regulates cell clustering. This study reveals the inhibitory role of ecdysone signaling in suppressing the zinc-finger transcription factor Broad (Br) and highlights gene expression changes as Br is suppressed and cells become flattened using single-cell RNA sequencing data.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Biology
Fahad Khan, Pratibha Pandey, Niraj Kumar Jha, Mohammad Khalid, Shreesh Ojha
Summary: The study found that rutin exhibited dose-dependent anti-proliferative effects on Caski cervical cancer cells, inducing significant apoptosis through caspase-3/9 activation, ROS generation, and alteration in Bax/Bcl2 mRNA expression. Cell cycle analysis showed G0/G1 phase arrest associated with reduced expression of CDK4 and Cyclin D1. Gene expression analysis indicated that rutin treatment decreased Notch-1 and Hes-1 mRNA expression levels.
Article
Developmental Biology
Chhavi Sood, Virginia T. Justis, Susan E. Doyle, Sarah E. Siegrist
Summary: Notch signaling coordinates neuroblast intrinsic temporal programs with extrinsic nutrient cues to regulate quiescence in fruit flies. Reduction in Notch activity delays or bypasses quiescence, resulting in continuous neuroblast division. Notch and its ligand Delta are expressed in neuroblasts before entering quiescence, and Delta transactivates Notch in adjacent GMC daughters. Over time and with increasing Delta-expressing daughters, neuroblast Notch activity increases, leading to cell cycle exit and attenuation of Notch pathway activity in quiescent neuroblasts.
Article
Cell Biology
Jiahui Du, Junjun Jing, Yuan Yuan, Jifan Feng, Xia Han, Shuo Chen, Xiang Li, Weiqun Peng, Jian Xu, Thach-Vu Ho, Xinquan Jiang, Yang Chai
Summary: In this study, loss of Arid1a was found to affect differentiation and root length of tooth root progenitors, with Plagl1 potentially conferring functional specificity to Arid1a. Additionally, loss of Arid1a resulted in increased expression of Arid1b, which has a different role in regulating Hh signaling.
Article
Chemistry, Multidisciplinary
Jin-yi Liu, Wei-qi Fu, Xiang-jin Zheng, Wan Li, Li-wen Ren, Jin-hua Wang, Cui Yang, Guan-hua Du
Summary: Avasimibe demonstrated potent anticancer effects on human glioblastoma cells by inhibiting proliferation, inducing apoptosis, and regulating multiple signaling pathways. The drug showed promising potential as a chemotherapy treatment for glioblastoma.
ACTA PHARMACOLOGICA SINICA
(2021)
Article
Biochemistry & Molecular Biology
Anja C. Nagel, Dominik Mueller, Mirjam Zimmermann, Anette Preiss
Summary: The membrane-bound Notch regulator (Mnr) supports Notch signaling by promoting the cleavage of the Notch receptor, which in turn enhances Notch receptor activation. Overexpression of Mnr stimulates γ-secretase activity, while knockdown of Mnr inhibits this activity, demonstrating Mnr as a positive regulator of Notch signaling.
Article
Oncology
Haini Wang, Junli Zuo
Summary: Overexpressed survivin is associated with worse survival of several types of human tumors. In this study, the antitumor activity of shikonin in non-small-cell lung cancer (NSCLC) by regulating survivin pathway was investigated. Result showed that shikonin inhibited the NSCLC H1299 cell proliferation in a dose-dependent manner. Moreover, shikonin fits well with survivin by molecular docking. Shikonin also inhibited the mRNA expression and protein level of survivin in H1299 cells. Shikonin arrested H1299 cell cycle at the G0/G1 phase by regulating CDK/cyclin family members. In addition, shikonin regulated the expression of X-linked inhibitor of apoptosis- (XIAP-) mediated caspases 3 and 9, thus leading to the damage of mitochondrial membrane potential and induction of H1299 cell apoptosis. Overall, shikonin inhibited H1299 cell growth by inducing apoptosis and blocking the cell cycle. The underlying mechanism involves targeting survivin, which subsequently regulates the protein expression of XIAP/caspase 3/9, CDK2/4, and cyclin E/D1. Thus, shikonin, a survivin inhibitor, is a promising therapeutic strategy in NSCLC treatment.
ANALYTICAL CELLULAR PATHOLOGY
(2021)
Article
Pharmacology & Pharmacy
Ahmed Alafnan, Nasrin E. E. Khalifa, Talib Hussain, Mhdia Elhadi Osman
Summary: This study explores the anticancer effects of Cucurbitacin-B (Cur-B), a triterpenoid compound found in the Cucurbitaceae family, on androgen-dependent prostate cancer LNCaP cells. The results show that Cur-B inhibits cell viability, induces apoptosis and cell cycle arrest, and reduces the expression of the Notch signaling cascade in LNCaP cells. Cur-B has the potential to be a promising therapeutic agent for androgen-dependent prostate cancer.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Genetics & Heredity
Lylah D. Deady, Jianjun Sun
Article
Genetics & Heredity
Lylah D. Deady, Wei Shen, Sarah A. Mosure, Allan C. Spradling, Jianjun Sun
Article
Multidisciplinary Sciences
Elizabeth Knapp, Jianjun Sun
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Genetics & Heredity
Wei Shen, Jianjun Sun
G3-GENES GENOMES GENETICS
(2017)
Article
Biology
Lylah D. Deady, Wei Li, Jianjun Sun
Article
Multidisciplinary Sciences
Wei Li, Jessica F. Young, Jianjun Sun
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Biochemistry & Molecular Biology
Jianjun Sun, Allan C. Spradling
Article
Developmental Biology
John S. Poulton, Yi-Chun Huang, Laila Smith, Jianjun Sun, Nicholas Leake, Justin Schleede, Leslie M. Stevens, Wu-Min Deng
Article
Biology
Jianjun Sun, Allan C. Spradling
Article
Developmental Biology
Elizabeth M. Knapp, Wei Li, Jianjun Sun
Article
Developmental Biology
Wei Shen, Jianjun Sun
Article
Biology
Elizabeth M. Knapp, Wei Li, Vijender Singh, Jianjun Sun
Article
Multidisciplinary Sciences
Kewa Jiang, Jiyang Zhang, Yuping Huang, Yingzheng Wang, Shuo Xiao, M. Kyle Hadden, Teresa K. Woodruff, Jianjun Sun
Summary: There is a significant unmet need for new contraceptive options for both women and men due to various reasons. However, using Drosophila as a model, potential compounds have been identified as nonsteroidal contraceptive candidates, showing promise for the development of novel contraceptive agents.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Developmental Biology
Rebecca Oramas, Elizabeth M. Knapp, Baosheng Zeng, Jianjun Sun
Summary: In this study, it was found that both Sim and Tgo transcription factors play important roles in promoting follicle maturation and ovulation in Drosophila.