期刊
MOLECULAR BIOLOGY AND EVOLUTION
卷 31, 期 9, 页码 2331-2341出版社
OXFORD UNIV PRESS
DOI: 10.1093/molbev/msu177
关键词
chromosomal inversion; inversion breakpoints; breakpoint reuse; Drosophila
资金
- Barcelona Subobscura Initiative (BSI)
- Ministerio de Economia y Competitividad, Spain [BFU2012-35168]
- Comissio Interdepartamental de Recerca i Innovacio Tecnologica, Generalitat de Catalunya, Spain [2009SGR-1287]
Inversions are an integral part of structural variation within species, and they play a leading role in genome reorganization across species. Work at both the cytological and genome sequence levels has revealed heterogeneity in the distribution of inversion breakpoints, with some regions being recurrently used. Breakpoint reuse at the molecular level has mostly been assessed for fixed inversions through genome sequence comparison, and therefore rather broadly. Here, we have identified and sequenced the breakpoints of two polymorphic inversions-E-1 and E-2 that share a breakpoint-in the extant E-st and E(1+2) under bar chromosomal arrangements of Drosophila subobscura. The breakpoints are two medium-sized repeated motifs that mediated the inversions by two different mechanisms: E-1 via staggered breaks and subsequent repair and E-2 via repeat-mediated ectopic recombination. The fine delimitation of the shared breakpoint revealed its strict reuse at the molecular level regardless of which was the intermediate arrangement. The occurrence of other rearrangements in the most proximal and distal extended breakpoint regions reveals the broad reuse of these regions. This differential degree of fragility might be related to their sharing the presence outside the inverted region of snoRNA-encoding genes.
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