期刊
MOLECULAR BIOLOGY
卷 52, 期 4, 页码 621-628出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0026893318040088
关键词
Fungal genome; CTAB; PEG; polysaccharides; Schizophyllum commune
资金
- National Natural Science Foundation of China [31471810, 31272081]
Polysaccharides influence concentration and purity of extracted DNA. Here we present rapid and efficient protocol for DNA extraction from samples rich in polysaccharides. The technique has been developed using cultures of Schizophyllum commune and involves a modification of known Cetyltrimethyl Ammonium Bromide (CTAB) protocol. To remove polysaccharides, Polyethylene Glycol (PEG) 8000 was added during DNA precipitation. Genomic DNA obtained with the CTAB-PEG method had high integrity, with average fragment size > 30 kb, the concentration higher than 100 ng/mu L, and the yield more than 30 mu g/g. Presented technique is suitable for DNA extraction from fungi, bacteria, archaea or even mollusks with high polysaccharide content.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据