Effect of extracellular recombinant human heat shock protein 70 (HSP70) on protein pattern observed after endotoxin-induced macrophage activation

The protein pattern of murine macrophages cell line J774 has been investigated using 2D electrophoresis after combined action of bacterial endotoxins (LPS), heat shock treatment (HS), and administration of recombinant human Hsp70. The investigation demonstrated significant protective effect of HS and recombinant Hsp70 treatment applied before LPS introduction. This effect is apparently realized by means of several signal transduction systems. In the course of the investigation, we identified eight proteins that exhibited pronounced changes in their synthesis due to combined treatment. The data accumulated may shed light on molecular mechanisms underlying protective antiseptic action of HS and/or recombinant Hsp70 applied before LPS administration.