期刊
MOLECULAR AND CELLULAR PROBES
卷 25, 期 4, 页码 168-173出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2011.04.003
关键词
STD; C. trachomatis; ompA genotypes; Strain D/Ep6; Mixed infection; Chlamydophila abortus; ArrayTube (TM) microarray assay
类别
资金
- ACIP (Inter-Pasteurien Concerted Actions, Institut Pasteur International)
PCR amplification and nucleotide sequencing of the ompA gene of Chlamydia trachomatis were used to determine the prevalence and distribution of genotypes in 51 urine and urethral specimens from Greek male patients with urethritis, that were positive by the COBAS Amplicor test A single C. trachomatis serovar was identified in 43 of the 51 amplified samples. Serovars F and E were the most prevalent (both 12, 28%), followed by D (9, 21%), G (4, 9%), B and K (both 2,5%) and H and J (both 1, 2%). Over one third of the samples bared a variant ompA genotype that had been previously identified in other areas worldwide. Two results in this study, both observed for the first time, were of particular interest. First, the emergence of the unique variant genotype D/Ep6 (X77364.2) identified in 3 urethral samples. Second, the ompA genotype OCLH196 of the animal pathogen Chlamydophila abortus as well as a 235 rRNA gene fragment of this species detected by the assay ArrayTube (TM), was found in 7 urethral samples. The implications resulting from this observation for the health of the general population are discussed. (C) 2011 Elsevier Ltd. All rights reserved.
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