Article
Microbiology
L. Ruffier d'Epenoux, E. Tessier, A. Guillouzouic, E. Fayoux, C. Bourigault, P. Bemer, S. Corvec
Summary: There is a possibility of introducing a new molecular test in the routine diagnosis of bacterial vaginosis. This study proposes a combination of Nugent scoring and molecular testing for diagnosing bacterial vaginosis, especially for cases with intermediate Nugent scores.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Pragati Grover Sehgal, Rajneesh Dadwal, Bhawna Sharma, Amit Sehgal, Rashmi Bagga, Seema Chopra, Archana Angrup, Rakesh Yadav, Nandita Sharma, Sunil Sethi
Summary: The study found a high sensitivity, specificity, negative predictive value, and odds ratio for the detection of co-infection of A. vaginae and G. vaginalis for the diagnosis of BV.
Article
Pathology
Aurore Loquet, Remi Le Guern, Teddy Grandjean, Claire Duployez, Marvin Bauduin, Eric Kipnis, Gilles Brabant, Damien Subtil, Rodrigue Dessein
Summary: This study utilized high-throughput quantitative PCR to screen 34 microorganisms in asymptomatic pregnant women and identified a combination of A. vaginae, Gardnerella spp., L. crispatus, and P. bivia as the optimal bacteria combination for diagnosing BV, with a sensitivity of 77.1% and specificity of 97.0% compared to the Nugent score. The quantitative PCR method implemented in this study provides a reproducible and easily assessible way of identifying the absence of BV in pregnant women.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2021)
Article
Cell Biology
Michael L. Neugent, Ashwani Kumar, Neha V. Hulyalkar, Kevin C. Lutz, Vivian H. Nguyen, Jorge L. Fuentes, Cong Zhang, Amber Nguyen, Belle M. Sharon, Amy Kuprasertkul, Amanda P. Arute, Tahmineh Ebrahimzadeh, Nitya Natesan, Chao Xing, Vladimir Shulaev, Qiwei Li, Philippe E. Zimmern, Kelli L. Palmer, Nicole J. De Nisco
Summary: Postmenopausal women are greatly affected by recurrent urinary tract infection (rUTI). This study identifies changes in the urogenital microbiome composition and functions that are associated with rUTI susceptibility in postmenopausal women. It also reveals an enrichment of lactobacilli in postmenopausal women taking estrogen hormone therapy.
CELL REPORTS MEDICINE
(2022)
Article
Infectious Diseases
Aliona S. Rosca, Joana Castro, Lucia G. Sousa, Angela Franca, Mario Vaneechoutte, Nuno Cerca
Summary: This study investigated the role of interactions between bacterial species in multi-species biofilms in bacterial vaginosis (BV) in relation to antimicrobial tolerance. The results showed that when these species were co-cultured, they could synergistically increase resistance to antibiotics.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Microbiology
Joana Castro, Aliona S. Rosca, Christina A. Muzny, Nuno Cerca
Summary: This study analyzed the impact of A. vaginae and P. bivia on a pre-established G. vaginalis biofilm and found that although they were unable to increase the total biofilm biomass, they significantly influenced the expression of key virulence genes of G. vaginalis. The microbial relationships between co-infecting bacteria can deeply affect the G. vaginalis biofilm, which is a crucial marker of BV.
Article
Biotechnology & Applied Microbiology
Tongyang Deng, Anquan Shang, Ying Zheng, Lianzhen Zhang, Hong Sun, Wei Wang
Summary: The aim of this study was to explore a new marker for the quantitative detection of bacterial vaginosis (BV) with high sensitivity and specificity. By comparing the differences in bacterial abundance between BV, healthy, and BV-intermediate groups using 16S rRNA-sequencing, candidate markers were identified. Real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was then used to quantitatively detect these candidate markers and evaluate their sensitivity and specificity. Four new indicators for BV detection were discovered, with log L. crispatus/G. vaginalis (L/G) < 0 being the best indicator. A molecular diagnostic system independent of the Nugent Score could have an important impact on the clinical management of BV.
Article
Infectious Diseases
Joana Castro, Lucia G. V. Sousa, Angela Franca, Lenka Podpera Tisakova, Lorenzo Corsini, Nuno Cerca
Summary: Bacterial vaginosis (BV) is a common vaginal infection in reproductive-age women. This study investigated the role of an investigational engineered phage endolysin in eradicating biofilms associated with BV.
Review
Immunology
Xiaodi Chen, Yune Lu, Tao Chen, Rongguo Li
Summary: The vaginal microbiome is crucial for women's health, with bacterial vaginosis (BV) associated with various adverse gynecologic and obstetric outcomes. Research on diagnosis and treatment strategies for BV is making progress.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Pawel Laniewski, Melissa M. Herbst-Kralovetz
Summary: This study demonstrated that 3D cervical epithelial cells colonized with cervicovaginal microbiota faithfully reproduce the immunometabolic microenvironment previously observed in clinical studies and can successfully be used as a robust tool to evaluate host responses to commensal and pathogenic bacteria in the female reproductive tract.
NPJ BIOFILMS AND MICROBIOMES
(2021)
Article
Pediatrics
Florence Bretelle, Sandrine Loubiere, Raoul Desbriere, Anderson Loundou, Julie Blanc, Helene Heckenroth, Thomas Schmitz, Alexandra Benachi, Bassam Haddad, Franck Mauviel, Xavier Danoy, Pierre Mares, Nawal Chenni, Jean-Pierre Menard, Jean-Francois Cocallemen, Nadia Slim, Marie Victoire Senat, Celine Chauleur, Caroline Bohec, Gilles Kayem, Cynthia Trastour, Andre Bongain, Patrick Rozenberg, Valerie Serazin, Florence Fenollar
Summary: This study evaluated the clinical and economic effects of point-of-care quantitative real-time polymerase chain reaction screening and treatment for bacterial vaginosis (BV) in low-risk pregnant women on preterm birth rates. The results showed that this strategy did not significantly reduce preterm birth rates or total costs compared to usual care. However, it was found to be more effective in low-risk nulliparous women.
Article
Microbiology
Victoria Horrocks, Charlotte K. Hind, Matthew E. Wand, Paul-Enguerrand Fady, Joel Chan, Jade C. Hopkins, Georgina L. Houston, Rachel M. Tribe, J. Mark Sutton, A. James Mason
Summary: Bacterial vaginosis (BV) is a common vaginal infection in women of childbearing age, increasing the risk of sexually transmitted infections and preterm delivery. This study investigates the metabolic diversity of BV-associated bacteria and lactobacilli, and the symbiotic relationships between them, to understand the mechanism and approach to risk prediction in BV and/or preterm birth.
Article
Pediatrics
Florence Bretelle, Sandrine Loubiere, Raoul Desbriere, Anderson Loundou, Julie Blanc, Helene Heckenroth, Thomas Schmitz, Alexandra Benachi, Bassam Haddad, Franck Mauviel, Xavier Danoy, Pierre Mares, Nawal Chenni, Jean-Pierre Menard, Jean-Francois Cocallemen, Nadia Slim, Marie Victoire Senat, Celine Chauleur, Caroline Bohec, Gilles Kayem, Cynthia Trastour, Andre Bongain, Patrick Rozenberg, Valerie Serazin, Florence Fenollar
Summary: This study aimed to evaluate the clinical and economic effects of point-of-care quantitative real-time polymerase chain reaction screen and treat for bacterial vaginosis (BV) in low-risk pregnant women on preterm birth. The results showed that this strategy did not significantly reduce the rate of preterm birth compared to usual care.
Article
Veterinary Sciences
Jinok Kwak, Sriniwas Pandey, Jinho Cho, Minho Song, Eun Sol Kim, Hyunok Doo, Gi Beom Keum, Sumin Ryu, Yejin Choi, Juyoun Kang, Sheena Kim, Jeongyun Kim, Hyeun Bum Kim
Summary: This study developed a mouse model for human BV induced by G. vaginalis, and demonstrated comparable clinical attributes to patients with BV.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Obstetrics & Gynecology
Agnieszka Kolakowska, Fatma El Alaoui, Heida Ben Alba, Salima Challal, Etienne Carbonnelle, Francoise Jaureguy
Summary: The incidence of extra pelvic infections due to vaginal microflora bacteria has increased. However, there is little knowledge about the risk factors, clinical presentation, treatment, and outcome of bone infections. We describe a case of spondylodiscitis caused by polymicrobial vaginal flora, which recovered after antibiotic treatment.
ARCHIVES OF GYNECOLOGY AND OBSTETRICS
(2022)
Article
Multidisciplinary Sciences
Kristen E. Pascal, Christopher M. Coleman, Alejandro O. Mujica, Vishal Kamat, Ashok Badithe, Jeanette Fairhurst, Charleen Hunt, John Strein, Alexander Berrebi, Jeanne M. Sisk, Krystal L. Matthews, Robert Babb, Gang Chen, Ka-Man V. Lai, Tammy T. Huang, William Olson, George D. Yancopoulos, Neil Stahl, Matthew B. Frieman, Christos A. Kyratsous
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Immunology
Erika K. Libby, Kristen E. Pascal, Eli Mordechai, Martin E. Adelson, Jason P. Trama
MICROBES AND INFECTION
(2008)
Article
Immunology
David W. Hilbert, Kristen E. Pascal, Erika K. Libby, Eli Mordechai, Martin E. Adelson, Jason P. Trama
MICROBES AND INFECTION
(2008)