期刊
MOLECULAR AND CELLULAR NEUROSCIENCE
卷 46, 期 1, 页码 32-44出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.mcn.2010.07.015
关键词
Dorsal root ganglion; Transcription factor; High content analysis; Screen; Systems biology; Cerebellar granule neuron; STAT3
资金
- Miami Project to Cure Paralysis
- Buoniconti Fund [DOD W81XWH-05-1-0061]
- Paralyzed Veterans of America Research Foundation [2396]
- NIH [HD057632, NS059866]
- Lois Pope LIFE Scholar award
Neurons in the peripheral nervous system (PNS) display a higher capacity to regenerate after injury than those in the central nervous system, suggesting cell specific transcriptional modules underlying axon growth and inhibition. We report a systems biology based search for PNS specific transcription factors (TFs). Messenger RNAs enriched in dorsal root ganglion (DRG) neurons compared to cerebellar granule neurons (CGNs) were identified using subtractive hybridization and DNA microarray approaches. Network and transcription factor binding site enrichment analyses were used to further identify TFs that may be differentially active. Combining these techniques, we identified 32 TFs likely to be enriched and/or active in the PNS. Twenty-five of these TFs were then tested for an ability to promote CNS neurite outgrowth in an overexpression screen. Real-time PCR and immunohistochemical studies confirmed that one representative TF, STAT3, is intrinsic to PNS neurons, and that constitutively active STAT3 is sufficient to promote CGN neurite outgrowth. (C) 2010 Elsevier Inc. All rights reserved.
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