Article
Multidisciplinary Sciences
Wencan Zhang, Xu Cao, Xiancai Zhong, Hongmin Wu, Mingye Feng, Yousang Gwack, Isakov Noah, Zuoming Sun
Summary: This study reveals the important role of steroid nuclear receptor coactivator 2 (SRC2) in regulating the differentiation of regulatory T cells (T-regs). Deficiency of SRC2 in T cells leads to defective T-reg differentiation and the development of autoimmune phenotypes. SRC2 promotes T-reg differentiation through the regulation of Nr4a2 and Foxp3 expression.
Article
Multidisciplinary Sciences
Yue Xiao, Min Hu, Qiyan Lin, Ting Zhang, Siying Li, Linjuan Shu, Xiuli Song, Xiaoyong Xu, Wentong Meng, Xue Li, Hong Xu, Xianming Mo
Summary: This study reveals that Dopey2 and Pcdh7 regulate the proliferation and differentiation of neural stem cells and progenitors during embryonic neurogenesis, leading to the proper size and architecture of zebrafish brains. Dopey2 promotes the proliferation of neural stem cells/progenitors, while Pcdh7 promotes their differentiation. These proteins work together to orchestrate the development and arrangement of neural cells in zebrafish embryonic brains.
Article
Biochemistry & Molecular Biology
Akiko Terauchi, Patricia Yee, Erin M. Johnson-Venkatesh, Mariel P. Seiglie, Lisa Kim, Julia C. Pitino, Eli Kritzer, Qiyu Zhang, Jie Zhou, Yulong Li, David D. Ginty, Wei-Chung A. Lee, Hisashi Umemori
Summary: Dopaminergic projections play a role in various brain functions and are involved in neuropsychiatric disorders. Two groups of TGF-(3 family members regulate the development of dopaminergic synapses in nigrostriatal and mesolimbic neurons. Specific activation of Smad1 and Smad2 is required for the development and function of dopaminergic synapses in nigrostriatal and mesolimbic projections.
Article
Cell Biology
Hellen Weinschutz Mendes, Uma Neelakantan, Yunqing Liu, Sarah E. Fitzpatrick, Tianying Chen, Weimiao Wu, April Pruitt, David S. Jin, Priyanka Jamadagni, Marina Carlson, Cheryl M. Lacadie, Kristen D. Enriquez, Ningshan Li, Dejian Zhao, Sundas Ijaz, Catalina Sakai, Christina Szi, Brendan Rooney, Marcus Ghosh, Ijeoma Nwabudike, Andrea Gorodezky, Sumedha Chowdhury, Meeraal Zaheer, Sarah McLaughlin, Joseph M. Fernandez, Jia Wu, Jeffrey A. Eilbott, Brent Vander Wyk, Jason Rihel, Xenophon Papademetris, Zuoheng Wang, Ellen J. Hoffman
Summary: This study performs parallel in vivo functional analysis of 10 ASD genes at the behavioral, structural, and circuit levels in zebrafish mutants, revealing both unique and overlapping effects of gene loss of function. Whole-brain mapping identifies the forebrain and cerebellum as the most significant contributors to brain size differences, while regions involved in sensory-motor control, particularly dopaminergic regions, are associated with altered baseline brain activity. Finally, a global increase in microglia resulting from ASD gene loss of function in select mutants implicates neuroimmune dysfunction as a key pathway relevant to ASD biology.
Article
Cell Biology
Jia Shang, Bin Li, Han Fan, Peidi Liu, Wen Zhao, Tao Chen, Pu Chen, Longqiu Yang
Summary: This study investigated the effects of sevoflurane on the midbrain's development using human physiologically-relevant midbrain organoids. The results showed that long-term exposure to sevoflurane could promote premature differentiation of midbrain organoids and inhibit cell proliferation, indicating a potential adverse effect on the midbrain development in fetuses.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Neurosciences
Sandrine Bataille, Hadrien Jalaber, Ingrid Colin, Damien Remy, Pierre Affaticati, Cynthia Froc, Jean-Pierre Levraud, Philippe Vernier, Michael Demarque
Summary: This study reveals that neuronal communication starts during the embryonic period before the establishment of synapses, and that it can affect the specification and quantity of dopamine neurons through modulating embryonic neural excitability (ENE). Manipulating ENE can result in an increase or decrease in dopamine neuron numbers, as well as impact the locomotion of larvae.
Article
Multidisciplinary Sciences
Jing Zhou, Xingli Zhang, Jiajia Hu, Rihao Qu, Zhibin Yu, Hao Xu, Huifang Chen, Lichong Yan, Chenbo Ding, Qiang Zou, Youqiong Ye, Zhengting Wang, Richard A. Flavell, Hua-Bing Li
Summary: The m(6)A eraser ALKBH5 plays a crucial role in controlling the pathogenicity of CD4(+) T cells during autoimmunity, as its deficiency results in weakened immune responses and reduced recruitment of neutrophils into the central nervous system.
Article
Neurosciences
Vladimir A. Ilin, Qing Bai, Alan M. Watson, Maxim Volgushev, Edward A. Burton
Summary: Zebrafish DC2/4 neurons share many physiological properties with mammalian dopaminergic neurons, including the cell-autonomous generation of action potentials. However, in contrast to mammalian dopaminergic neurons, the pacemaker driving intrinsic rhythmic activity in zebrafish DC2/4 neurons does not involve calcium conductances, HCN channels, or sodium leak currents.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Chee Ern David Wong, Khang Hua, Simon Monis, Vishal Saxena, Anwar Norazit, Suzita Mohd Noor, Marc Ekker
Summary: The study demonstrates that gdnf in zebrafish plays a crucial role in the survival and differentiation of dopaminergic neurons, and its deficiency results in reduced dopaminergic neuron numbers and neurogenesis.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Neurosciences
Yuganthini Vijayanathan, Naemah Md Hamzah, Siong Meng Lim, Fei Ting Lim, Maw Pin Tan, Abu Bakar Abdul Majeed, Kalavathy Ramasamy
Summary: This study investigated the specific phases of neuroregeneration in a time-based manner in an adult zebrafish-based Parkinson's disease model. The results showed a downregulation of gene and protein expression at day three and nine postlesion, a transient increase of cell proliferation in the olfactory bulb and telencephalon at day seven postlesion, and a significant increase of cell proliferation and activated astrocytes at day 14 postlesion in the lesion area. Furthermore, at day 30 postlesion, the proliferative cells matured into dopaminergic neurons.
BRAIN RESEARCH BULLETIN
(2022)
Article
Environmental Sciences
Mahesh Rachamalla, Arash Salahinejad, Maria Khan, Ashok Kumar Datusalia, Som Niyogi
Summary: The study aimed to evaluate the effects of chronic dietary arsenic exposure on the cognitive performance of adult zebrafish and uncover the possible mechanisms behind these neurotoxic effects. The results showed that dietary treatment with arsenic significantly impaired the fish's performance in various latent learning tasks. Chronic dietary exposure to arsenic also increased arsenic accumulation and dopamine levels, and induced oxidative stress in the zebrafish brain. The study suggests that chronic environmentally relevant dietary exposure to arsenic can impair cognitive performance in zebrafish by inducing oxidative stress and disrupting dopaminergic neurotransmission.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Neurosciences
Adele Leggieri, Judit Garcia-Gonzalez, Jose V. Torres-Perez, William Havelange, Saeedeh Hosseinian, Aleksandra M. Mech, Marcus Keatinge, Elisabeth M. Busch-Nentwich, Caroline H. Brennan
Summary: ANKK1, a protein involved in cell proliferation, differentiation, and transcription factor activation, is suggested to play a role in vulnerability to addictions. It may affect the development and functioning of dopaminergic pathways, and has a functional relationship with the dopamine receptor DRD2.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Taichi Suzuki, Yo Hirai, Tomoko Uehara, Rie Ohga, Kenjiro Kosaki, Atsuo Kawahara
Summary: Studies on trrap gene-knockout zebrafish mutants revealed smaller eyes and heads, reduced size of ventral pharyngeal arches, impaired teeth mineralization, and altered expression of dlx3 and dlx2b, suggesting these mutants may serve as a useful model for human disorders associated with genetic mutations in the TRRAP gene.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Brianna Peskin, James Norman, Jennifer Bagwell, Adam Lin, Priyom Adhyapok, Stefano Di Talia, Michel Bagnat
Summary: The vertebrate spine is composed of alternating vertebral bodies and intervertebral discs. Recent studies in zebrafish have found that the sheath surrounding the notochord differentiates into cartilage-like and mineralizing segments, which serve as a blueprint for vertebral body formation. The activation of Notch signaling is essential for sequential notochord segmentation, but the mechanism of activation and the molecular components involved remain unclear. This study reveals that BMP signaling precedes Notch activation and plays a role in segment growth and spine morphogenesis.
Article
Multidisciplinary Sciences
Zigang Cao, Yunlong Meng, Fanghua Gong, Zhaopeng Xu, Fasheng Liu, Mengjie Fang, Lufang Zou, Xinjun Liao, Xinjuan Wang, Lingfei Luo, Xiaokun Li, Huiqiang Lu
Summary: This study reveals that calcineurin inhibition can induce proximal growth of regeneration blastema in zebrafish fins, establishing calcineurin as a molecular switch for specifying the blastema of the proximodistal axis in fin regeneration polarity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Chirag Nepal, Marion Coolen, Yavor Hadzhiev, Delphine Cussigh, Piotr Mydel, Vidar M. Steen, Piero Carninci, Jesper B. Andersen, Laure Bally-Cuif, Ferenc Muller, Boris Lenhard
NUCLEIC ACIDS RESEARCH
(2016)
Review
Developmental Biology
Ajay Chitnis, Laure Balle-Cuif
Article
Developmental Biology
Carl Breacker, Iain Barber, William H. J. Norton, Jonathan R. McDearmid, Ceinwen A. Tilley
Article
Developmental Biology
Emmanuel Than-Trong, Sara Ortica-Gatti, Sebastien Mella, Chirag Nepal, Alessandro Alunni, Laure Bally-Cuif
Article
Biochemical Research Methods
Hector Carreno Gutierrez, Irene Vacca, Anna Inguanzo Pons, William H. J. Norton
JOURNAL OF NEUROSCIENCE METHODS
(2018)
Article
Biochemistry & Molecular Biology
Angelo Bitetti, Allison C. Mallory, Elisabetta Golini, Claudia Carrieri, Hector Carreno Gutierrez, Emerald Perlas, Yuvia A. Perez-Rico, Glauco P. Tocchini-Valentini, Anton J. Enright, William H. J. Norton, Silvia Mandillo, Donal O'Carroll, Alena Shkumatava
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2018)
Article
Clinical Neurology
Merlin Lange, Cynthia Froc, Hannah Grunwald, William H. J. Norton, Laure Bally-Cuif
PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY
(2018)
Article
Biochemistry & Molecular Biology
Angelo Bitetti, Allison C. Mallory, Elisabetta Golini, Claudia Carrieri, Hector Carreno Gutierrez, Emerald Perlas, Yuvia A. Perez-Rico, Glauco P. Tocchini-Valentini, Anton J. Enright, William H. J. Norton, Silvia Mandillo, Donal O'Carroll, Alena Shkumatava
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Marion Coolen, Miriam Labusch, Abdelkrim Mannioui, Laure Bally-Cuif
Article
Biochemical Research Methods
Ramona Corman, Willem Boutu, Anna Campalans, Pablo Radicella, Joana Duarte, Maria Kholodtsova, Laure Bally-Cuif, Nicolas Dray, Fabrice Harms, Guillaume Dovillaire, Samuel Bucourt, Hamed Merdji
BIOMEDICAL OPTICS EXPRESS
(2020)
Article
Multidisciplinary Sciences
Emmanuel Than-Trong, Bahareh Kiani, Nicolas Dray, Sara Ortica, Benjamin Simons, Steffen Rulands, Alessandro Alunni, Laure Bally-Cuif
Review
Biochemistry & Molecular Biology
Nicolas Dray, Emmanuel Than-Trong, Laure Bally-Cuif
Summary: Adult stem cell populations must coordinate their own maintenance and the generation of differentiated cell types in a spatially controlled manner to sustain organ physiology. Feedback mechanisms coordinate stem cell maintenance and progeny generation in epithelia, while little is known about potential community processes underlying neural stem cell maintenance. Recent work on neural stem cell pools suggests that homeostasis may rely on population asymmetry and long-term spatiotemporal coordination of neural stem cell states and fates, indicating neural stem cell assemblies in the vertebrate brain behave as self-organized systems.
Article
Biology
Sebastien Herbert, Leo Valon, Laure Mancini, Nicolas Dray, Paolo Caldarelli, Jerome Gros, Elric Esposito, Spencer L. Shorte, Laure Bally-Cuif, Nathalie Aulner, Romain Levayer, Jean-Yves Tinevez
Summary: Quantitative imaging of epithelial tissues requires accurate and widely applicable bioimage analysis tools. This paper introduces a user-friendly toolbox that can robustly project epithelia on their 2D surface from 3D volumes, producing accurate morphology measurements corrected for projection distortion. The toolbox consists of the LocalZProjector Fiji plugin and the DeProj MATLAB tool, which combine to effectively deproject the 2D segmentation in 3D, demonstrating effectiveness across various biological samples.
Article
Biochemistry & Molecular Biology
Giacomo Furlan, Valentina Cuccioli, Nelly Vuillemin, Lara Dirian, Anna Janue Muntasell, Marion Coolen, Nicolas Dray, Sebastien Bedu, Corinne Houart, Emmanuel Beaurepaire, Isabelle Foucher, Laure Bally-Cuif