期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 388, 期 1-2, 页码 41-50出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2014.03.004
关键词
Regeneration; Diabetes; Cell therapy; Paracrine signaling
资金
- Research Fund for the Doctoral Program of Higher Education of China [20090071110019]
- Shanghai Municipal Government [09ZR1405900]
Transplantation of bone marrow mesenchymal stem cells (MSCs) has been shown to effectively lower blood glucose levels in diabetic individuals, but the mechanism has not been adequately explained. We hypothesized that MSCs exert beneficial paracrine actions on the injured islets by releasing biologically active factors. To prove our hypothesis, we tested the cytoprotective effect of conditioned medium from cultured MSCs on isolated islets exposed to STZ in vitro and on mice islets after the experimental induction of diabetes in vivo. We assessed islet regeneration in the presence of conditioned medium and explored the possible mechanisms involved. Transplantation of MSCs can ameliorate hyperglycemia in diabetic mice by promoting the regeneration of 13 cells. Both p cell replication and islet progenitors differentiation contribute to 3 cell regeneration. MSC transplantation resulted in increases in pAkt and pErk expression by islets in vivo. Treatment with MSC-CM promoted islet cell proliferation and resulted in increases in pAkt and pErk expression by islets in vitro. The -MSC-CM-mediated induction of (3 cell proliferation was completely blocked by the PI3K/Akt inhibitor LY294002 but not by the MEK/Erk inhibitor PD98059. Together, these data suggest that the PI3K/Akt signal pathway plays a critical role in beta cell proliferation after MSC transplantation. (c) 2014 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据