期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 382, 期 1, 页码 244-253出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2013.10.014
关键词
MicroRNA-224; Mouse cumulus expansion; Pbc3; EGF
资金
- National Natural Science Foundation of China [31171379, 81125005]
- Chinese Academy of Sciences Knowledge Creative Program [KSCX2-EW-R-07]
MicroRNAs (miRNAs) are indicated to regulate ovarian development in a cell- or stage-specific manner. Our previous study showed that miR-224 is involved in TGF-beta 1-mediated follicular granulosa cell (GC) growth and estradiol (E2) production during the transition from the preantral to early antral stage by targeting Smad4. In this study, miR-224 was found to target pentraxin 3 (Ptx3), a gene critical for cumulus expansion during ovulation. In addition, PTX3 was up-regulated in mouse mural GCs and cumulus-oocyte complexes (COCs) by TGF-beta 1 treatment, which was partially mediated by miR-224. The effect of miR-224 during ovulation was further examined in vitro and in vivo by construction of an adenovirus-mediated expression vector for miR-224 (Ad-miR-224). In vitro studies demonstrated that miR-224 could perturb cumulus expansion in EGF-stimulated COCs by decreasing PTX3 secretion. In vivo studies also showed that injection of Ad-miR-224 into ovarian bursa decreased PTX3 expression and disrupted ovulation, which led to a decreased number of implantation sites and offspring being born. These results indicate that miR-224 may affect ovulation and subsequent embryo development by targeting Ptx3, suggesting potential roles for miRNAs in offering new treatments for ovulation disorder-associated infertility, or, conversely, designing new contraceptives. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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