期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 376, 期 1-2, 页码 33-42出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2013.05.025
关键词
Microtus oeconomus; Mouse; Hypoxia; Insulin-like growth factor; mRNA stability; U-rich element
资金
- Ministry of Science and Technology of China
- National Basic Research Program (973) of China [2012CB518200]
- National Natural Science Foundation of China [31071047, 30870300, 30770807]
Our previous study revealed the particular expression patterns of insulin-like growth factor 1 (IGF1) and insulin-like growth factor binding protein 1 (IGFBP1) in the Qinghai-Tibet plateau root vole (Microtus oeconomus) under hypoxic challenge. Here we report the molecular mechanisms of Igf gene regulation associated with adaptation to hypoxia. M. oeconomus IGF1 and IGFBP1 were shown to be highly conserved. Hypoxia (8.0% O-2, 6 h) did not change the liver-derived le expression in either M. oeconomus or mouse. Hypoxia significantly upregulated hepatic Igfbp1 gene expression and IGFBP1 levels in the liver and plasma of the mouse, but not in M. oeconomus. A functional U-rich element in the 3' untranslated region was found in mouse Igfbp1 mRNA, which was associated with Igfbp1 mRNA stabilization and upregulation under hypoxia, and this U-rich element was eliminated in the M. oeconomus Igfbp1, resulting in blunted Igfbp1 mRNA upregulation, which might be understood as a sequence variation modified during molecular evolution under hypoxia. (C) 2013 Published by Elsevier Ireland Ltd.
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