期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 341, 期 1-2, 页码 18-24出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2011.03.008
关键词
Postnatal fetal Leydig cells; Steroidogenesis; Steroidogenic gene expression
资金
- Swedish Research Council [8282]
- Swedish Children's Cancer Fund
- Frimurare Barnhuset Foundation in Stockholm
- Sallskapet Barnavard
- Stiftelsen Samariten
- Karolinska Institute
We studied steroidogenesis and the regulation of Leydig cell-specific gene expression in primary cultures of highly purified postnatal fetal Leydig cells (PFLCs). PFLCs activated by hCG and (Bu)(2)cAMP demonstrated transient capacity to produce testosterone (T) in vitro. A time dependent decline in T production by (Bu)(2)cAMP-stimulated PFLCs was observed and associated with the accumulation of progesterone in the culture media and complete suppression of P450c17 expression at the translational but not transcriptional level. PFLCs was found to lose their capacity to express Leydig cell-related genes (e.g., 3 beta HSD, P450c17, Ins13), which was restored by treatment with (Bu)(2)cAMP. It was also found that PDGF alpha alone and in combination with (Bu)(2)cAMP significantly stimulated proliferation of the isolated PFLCs in vitro. Our data indicate that cAMP-activated signaling pathway(s) play an important role in the regulation of PFLC differentiation and function. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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