Regulation of cell death in human fetal and adult ovaries-Role of Bok and Bcl-XL

Of eight million oocytes formed in fetal ovaries, only 400 are ovulated and the rest are degraded via apoptosis. Studies in rodents suggest an important role for Bok and Bcl-X-L. in ovarian apoptosis. but their expression patterns and roles in human ovaries are not well known. Protein expression of Bok and Bcl-X-L as well as the death pathway effectors TNF and caspase-3 were determined in an important collection of samples consisting of human fetal and adult ovaries. A penetrant expression of Bok, Bcl-X-L, TNF and full length and cleaved caspase-3 were characterized in fetal ovaries, with specific patterns in oocytes and pre-granulosa/granulosa cells. Bok and Bcl-X-L were detected also in adult ovaries. Lentiviral shRNA delivery demonstrated that loss of Bok markedly reduces vulnerability to apoptosis and, conversely, loss of Bcl-X-L increases apoptosis in human granulosa tumour cell line. The results suggest important roles for Bok and Bcl-X-L in human ovarian development, follicle maturation and apoptosis. (C) 2010 Elsevier Ireland Ltd. All rights reserved.