Article
Medicine, Research & Experimental
Mingtian Deng, Yongjie Wan, Baobao Chen, Xiangpeng Dai, Zifei Liu, Yingnan Yang, Yu Cai, Yanli Zhang, Feng Wang
Summary: This study reveals the crucial regulatory role of lnc_3712 in the development of goat SCNT embryos by repressing Kdm5b expression and affecting H3K4me3 demethylation process. Silencing of lnc_3712 enhances the development of SCNT embryos and shows similar transcriptional activity and expression of critical genes as in vitro fertilization embryos.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Biochemistry & Molecular Biology
Runsheng He, Besa Xhabija, Lijin K. Gopi, Jiji T. Kurup, Zhishan Xu, Zhe Liu, Benjamin L. Kidder
Summary: The H3K4 demethylase KDM5B is overexpressed in various cancers and its high expression is associated with reduced survival. Loss of KDM5B in cancer cells leads to increased proliferation and expression of cancer stem cell markers. KDM5B also plays a role in regulating epigenetic plasticity, leading to changes in chromatin states and activation or repression of alternative transcriptional programs.
Article
Multidisciplinary Sciences
Felicia Lazure, Rick Farouni, Korin Sahinyan, Darren M. Blackburn, Aldo Hernandez-Corchado, Gabrielle Perron, Tianyuan Lu, Adrien Osakwe, Jiannis Ragoussis, Colin Crist, Theodore J. Perkins, Arezu Jahani-Asl, Hamed S. Najafabadi, Vahab D. Soleimani
Summary: Adult stem cells play a crucial role in tissue regeneration, but their function declines with age. In this study, we investigated the impact of the stem cell niche environment on their function using muscle stem cells as a model. We found that aging resulted in significant changes in the gene expression of muscle stem cells, and exposure to a young niche environment restored the expression of a large portion of age-altered genes. This emphasizes the potential of targeting the stem cell niche as a therapeutic strategy for enhancing tissue regeneration in aging.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Xuetian Yue, Jianming Wang, Chun-Yuan Chang, Juan Liu, Xue Yang, Fan Zhou, Xia Qiu, Vrushank Bhatt, Jessie Yanxiang Guo, Xiaoyang Su, Lanjing Zhang, Zhaohui Feng, Wenwei Hu
Summary: This study found that overexpression of LIF in breast cancer promotes tumorigenesis by increasing glucose uptake and driving glycolysis. It also identifies LIF and its downstream signaling as potential therapeutic targets for breast cancers with LIF overexpression.
CELL DEATH & DISEASE
(2022)
Article
Medicine, Research & Experimental
Wei Liu, Xia Zhou, Qi Yao, Chen Chen, Qing Zhang, Keshuo Ding, Lu Li, Zhutian Zeng
Summary: Attenuated Escherichia coli producing CRISPR/CasΦ machinery enables efficient gene editing in Kupffer cells, providing a potential treatment for liver metastasis.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Cell & Tissue Engineering
Yujie Chen, Ruimin Xu, Shuang Zhou, Chengchen Zhao, Ziyue Hu, Yuwei Hua, Yanhong Xiong, Xiaoyu Liu, Junhong Lu, Yao Sun, Chong Li, Shaorong Gao, Yong Zhang
Summary: Cellular mechanical properties play a crucial role in cell fate transitions, but their relationship with transition efficiency and chromatin structure is not well understood. In this study, we found that mechanical strain treatment can induce cellular dedifferentiation and transdifferentiation in mouse cumulus cells (CCs), leading to improved somatic cell nuclear transfer reprogramming efficiency. Mechanistically, mechanical strain treatment increased chromatin accessibility in CCs, partly through the induction of the YAP-TEAD interaction. Moreover, using mechanical strain-treated CCs prevented transcriptional dysregulation in SCNT embryos. Overall, our findings suggest that manipulation of cell mechanical properties to regulate epigenetic status can facilitate cell fate transition.
Article
Cell Biology
Yohan Gerber-Ferder, Jason Cosgrove, Aleria Duperray-Susini, Yoann Missolo-Koussou, Marine Dubois, Kateryna Stepaniuk, Manuela Pereira-Abrantes, Christine Sedlik, Sonia Lameiras, Sylvain Baulande, Nathalie Bendriss-Vermare, Pierre Guermonprez, Diana Passaro, Leila Perie, Eliane Piaggio, Julie Helft
Summary: Infiltration of myeloid cells in solid tumors is associated with poor patient prognosis and disease severity. It is crucial to understand the regulation of myeloid cell differentiation during cancer in order to counteract their pro-tumorigenic role. In this study, researchers found that breast tumors can remotely remodel the bone marrow microenvironment and lead to increased myeloid differentiation.
NATURE CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Xiao-Jun Li, Charles Morgan, Loyal A. Goff, Angelika Doetzlhofer
Summary: This study found that transient activation of the progenitor-specific RNA binding protein LIN28B and Activin antagonist follistatin (FST) enhances regenerative competence of maturing/mature cochlear supporting cells (SCs) by reprogramming them into progenitor-like cells in organoid culture. LIN28B drives SC reprogramming, while FST is required to counterbalance hyperactivation of transforming growth factor-beta-type signaling by LIN28B.
Article
Genetics & Heredity
Shiyu An, Dan Yao, Wenyi Zhang, Hao Sun, Tianyi Yu, Ruizhe Jia, Yang Yang
Summary: This study discovered the involvement of WDR36 in self-renewal and differentiation potential of human EPS cells, with the effects being reversible by inhibiting P53.
FRONTIERS IN GENETICS
(2022)
Article
Multidisciplinary Sciences
Rawiya Al Hosni, Laurent Bozec, Scott J. Roberts, Umber Cheema
Summary: The biophysical microenvironment of the cell can be controlled using engineered scaffolds to manipulate cell signaling and function. This study demonstrates that culturing Adipose-derived Mesenchymal Stromal Cells (AMSC) in low collagen density environments under hypoxic conditions enhances cell potency. Differential cell fate responses are observed when varying collagen densities are combined with hypoxic conditions. The findings highlight the importance of the physical and physiological cell environment and present a chemically independent method for reprogramming and isolating skeletal progenitor cells from adipose-derived populations.
Article
Cell Biology
Yihuan Mao, Libin Wang, Bei Zhong, Ning Yang, Zhikun Li, Tongtong Cui, Guihai Feng, Wei Li, Ying Zhang, Qi Zhou
Summary: The study demonstrates that continuous expression of OSKM genes can induce and maintain mouse pluripotency without specific culturing factors. Experimental results show that iPSCs reprogrammed through OSKM expression can stably propagate and form various embryonic layers. Furthermore, OSKM-iPSCs contribute to germline transmission, with potential applications in gene editing in other species.
CELL PROLIFERATION
(2021)
Article
Biochemistry & Molecular Biology
Guangsuo Xing, Zichao Liu, Luyuan Huang, Danyun Zhao, Tao Wang, Hao Yuan, Yi Wu, Linpeng Li, Qi Long, Yanshuang Zhou, Zhihong Hao, Yang Liu, Jianghuan Lu, Shiting Li, Jieying Zhu, Bo Wang, Junwei Wang, Jing Liu, Jiekai Chen, Duanqing Pei, Xingguo Liu, Keshi Chen
Summary: The study revealed that Mkk6 acts as a chromatin relaxer during reprogramming by promoting histone acetylation levels and facilitating transcription factor binding, ultimately enhancing pluripotency gene expression.
CELL DEATH AND DIFFERENTIATION
(2022)
Editorial Material
Immunology
Aniqa B. Khan, Clinton S. Robbins
Summary: Mononuclear phagocyte proliferation can occur in the interstitial macrophage niche in the lung, beyond myeloid progenitor cells and mature macrophages.
Article
Immunology
Vincent Fregona, Manon Bayet, Mathieu Bouttier, Laetitia Largeaud, Camille Hamelle, Laura A. Jamrog, Nais Prade, Stephanie Lagarde, Sylvie Hebrard, Isabelle Luquet, Veronique Mansat-De Mas, Marie Nolla, Marlene Pasquet, Christine Didier, Ahmed Amine Khamlichi, Cyril Broccardo, Eric Delabesse, Stephane J. C. Mancini, Bastien Gerby
Summary: The PAX5::ELN oncogenic model disrupts differentiation of preleukemic cells and leads to the emergence of rare and quiescent pre-LSCs that reactivate an immature molecular program similar to chemo-resistant cells in B-ALL.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
David M. Zhang, Rachita Navara, Tiankai Yin, Jeffrey Szymanski, Uri Goldsztejn, Camryn Kenkel, Adam Lang, Cedric Mpoy, Catherine E. Lipovsky, Yun Qiao, Stephanie Hicks, Gang Li, Kaitlin M. S. Moore, Carmen Bergom, Buck E. Rogers, Clifford G. Robinson, Phillip S. Cuculich, Julie K. Schwarz, Stacey L. Rentschler
Summary: Noninvasive cardiac radiotherapy may effectively manage ventricular tachycardia in refractory patients by reprogramming cardiac conduction without transmural fibrosis, potentially offering a new treatment strategy for arrhythmia management.
NATURE COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Wai Lim Ku, Kosuke Nakamura, Weiwu Gao, Kairong Cui, Gangqing Hu, Qingsong Tang, Bing Ni, Keji Zhao
Article
Immunology
Chao Zhong, Mingzhu Zheng, Kairong Cui, Andrew J. Martins, Gangqing Hu, Dan Li, Lino Tessarollo, Serguei Kozlov, Jonathan R. Keller, John S. Tsang, Keji Zhao, Jinfang Zhu
Article
Cell & Tissue Engineering
Zhilin Ma, Jian Xu, Limei Wu, Junjie Wang, Qiqi Lin, Fabliha Ahmed Chowdhury, Habibul Hasan Mazumder, Gangqing Hu, Xue Li, Wei Du
Article
Oncology
Jae Young So, Nicolas Skrypek, Howard H. Yang, Anand S. Merchant, George W. Nelson, Wei-Dong Chen, Hiroki Ishii, Jennifer M. Chen, Gangqing Hu, Bhagelu R. Achyut, Esther C. Yoon, Liying Han, Chuanshu Huang, Margaret C. Cam, Keji Zhao, Maxwell P. Lee, Li Yang
Article
Cell Biology
Tingliang Wang, Ryan C. Hill, Monika Dzieciatkowska, Lian Zhu, Aniello M. Infante, Gangqing Hu, Kirk C. Hansen, Ming Pei
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Oncology
Tayvia Brownmiller, Jamie A. Juric, Abby D. Ivey, Brandon M. Harvey, Emily S. Westemeier, Michael T. Winters, Alyson M. Stevens, Alana N. Stanley, Karen E. Hayes, Samuel A. Sprowls, Amanda S. Gatesman Ammer, Mackenzee Walker, Erik A. Bey, Xiaoliang Wu, Zuan-Fu Lim, Lin Zhu, Sijin Wen, Gangqing Hu, Patrick C. Ma, Ivan Martinez
Review
Pharmacology & Pharmacy
Wei-Chih Chen, Gangqing Hu, Lori A. Hazlehurst
CURRENT OPINION IN PHARMACOLOGY
(2020)
Article
Engineering, Biomedical
Yiming Wang, Gangqing Hu, Ryan C. Hill, Monika Dzieciatkowska, Kirk C. Hansen, Xiao-Bing Zhang, Zuoqin Yan, Ming Pei
Summary: This study demonstrates that expansion on decellularized extracellular matrix (dECM) can rejuvenate high-passage infrapatellar fat pad stem cells (IPFSCs) by reversing cellular senescence. SV40LT transduced IPFSCs showed increased proliferation and adipogenic potential but decreased chondrogenic potential. Matrix components like basement membrane proteins were identified as key factors in matrix-mediated rejuvenation of IPFSCs.
Article
Multidisciplinary Sciences
Megan E. Grund, Soo J. Choi, Dudley H. McNitt, Mariette Barbier, Gangqing Hu, P. Rocco LaSala, Christopher K. Cote, Rita Berisio, Slawomir Lukomski
Article
Biochemistry & Molecular Biology
Weiwu Gao, Wai Lim Ku, Lixia Pan, Jonathan Perrie, Tingting Zhao, Gangqing Hu, Yuzhang Wu, Jun Zhu, Bing Ni, Keji Zhao
Summary: The novel indexing strategy of iscDNase-seq enables profiling of chromatin accessibility in individual cells, revealing epigenomic variability and regulatory elements among them. This technique accurately identifies cell types and predicts gene expression variability, showing higher correlation compared to scATAC-seq and scRNA-seq. iscDNase-seq provides a promising alternative method for single-cell epigenomics studies.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Mingzhu Zheng, Kairui Mao, Difeng Fang, Dan Li, Jun Lyu, Dingkang Peng, Xi Chen, Nikki Cannon, Gangqing Hu, Jiajia Han, Keji Zhao, Wanjun Chen, Jinfang Zhu
Summary: IgA production in the gut relies on IgA-producing plasma cells derived from conventional B cells, with T cells and helper-like ILCs playing crucial roles in the formation of lymphoid structures in the gut. However, the functions of non-LTi helper-like ILCs in promoting IgA production are still not well understood.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Osama M. Elzamzamy, Brandon E. Johnson, Wei-Chih Chen, Gangqing Hu, Reinhold Penner, Lori A. Hazlehurst
Summary: The study investigated the treatment mechanism of MTI-101 for multiple myeloma (MM) and found that MTI-101 can induce Ca2+ and Na+ flux through TRPC1 heteromers. The formation of TRPC1-calcium-regulating protein STIM1 complex plays a key role in MTI-101-induced cell death.
Article
Oncology
Sebastian A. Dziadowicz, Lei Wang, Halima Akhter, Drake Aesoph, Tulika Sharma, Donald A. Adjeroh, Lori A. Hazlehurst, Gangqing Hu
Summary: The bone marrow microenvironment can protect multiple myeloma (MM) cells from therapeutic agents, leading to drug resistance. This study aimed to understand the mechanisms of de novo multi-drug resistance induced by interactions between MM cells and bone marrow stromal cells (BMSCs). Through genome-wide analysis, we identified changes in gene expression and chromatin accessibility in MM cells interacting with BMSCs. We found that BMSC-derived soluble factors and physical adhesion can induce distinct changes in the transcriptome and regulome of MM cells. Furthermore, we identified candidate transcription factors that regulate the BMSC-induced transcriptome and modulate the regulome. These findings provide valuable insights into the epigenetic mechanisms of BMSC-induced multi-drug resistance in MM.
Article
Oncology
Clark Jones, Sebastian Dziadowicz, Samuel Suite, Ashley Eby, Wei-Chih Chen, Gangqing Hu, Lori A. Hazlehurst
Summary: MTI-101, a novel cyclic peptide, has shown anti-tumor activity in multiple myeloma and castrate-resistant prostate cancer models. It induces a change in cell state in lung cancer cell lines, leading to increased sensitivity to EGFR inhibitors and synergistic effects with standard of care agents. Additionally, it reduces bone metastasis occurrence. These findings highlight the potential therapeutic value of chronic MTI-101 treatment.
Article
Oncology
Lei Wang, Weijun Yi, Li Ma, Emily Lecea, Lori A. Hazlehurst, Donald A. Adjeroh, Gangqing Hu
Summary: This study found that bone marrow mesenchymal stem cells (MSCs) in multiple myeloma patients have tumor-supportive and inflammatory characteristics, contributing to drug resistance. In vitro expansion of MSCs leads to the loss of inflammatory signature, but cytokine stimulation and coculture with immune cells or cancer cells can reactivate these characteristics. These findings provide an important foundation for further research on the role of inflammation in the tumor-supportive functions of bone marrow MSCs in disease progression and therapy resistance.