Article
Biochemistry & Molecular Biology
Min Liang, Zhaohua Cai, Yangjing Jiang, Huanhuan Huo, Linghong Shen, Ben He
Summary: In this study, the researchers discovered that SUMOylation modification plays an important role in the function of myocardin, promoting the expression of smooth muscle-specific genes through increased stability of myocardin protein. They also found that SENP2 regulates SUMOylation modification and protein stability, and is involved in the phenotypic switching of VSMCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Andrology
Xiongcai Zhou, Chao Luo, Junhong Fan, Guangqian Gao, Tao Wang, Haibo Zhang, Anyang Wei
Summary: This study investigated the mechanism of phenotypic transformation of corporal cavernosum smooth muscle cells (CCSMCs) under hypoxic conditions. The results showed that HIF-1 alpha and Myocd played important roles in the phenotypic modulation of CCSMCs. Myocd overexpression could inhibit the phenotypic switch induced by hypoxia, suggesting it as a potential therapeutic target for erectile dysfunction under hypoxia or HIF-1 alpha activation.
WORLD JOURNAL OF MENS HEALTH
(2023)
Review
Cell Biology
Jiali Yao, Linqian Cai, Yingrui Chen, Jie Zhang, Wenwen Zhuang, Jingyan Liang, Hongliang Li
Summary: This review discusses the impact of Exosomes on atherosclerosis (AS) from different perspectives, focusing on the phenotypic transition of vascular smooth muscle cells (VSMCs). It provides insights into the current status and potential future directions in this field, offering new ideas for clinical research and treatment of AS.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Cell Biology
Danyang Shi, Jinhua Ding, Shouqiang Xie, Lei Huang, Hongmin Zhang, Xiaojie Chen, Xuejun Ren, Sa Zhou, Hongpeng He, Wenjian Ma, Tongcun Zhang, Nan Wang
Summary: MYOCD knockdown triggers autophagy and decreases the expression of contractile proteins in VSMCs. MYOCD activates the transcription of miR-30a, which targets ATG6 to regulate autophagy. PDGF-BB reduces miR-30a levels, while re-expression of miR-30a inhibits cell proliferation. Restoring miR-30a expression in an animal model prevents neointimal formation.
CELL DEATH & DISEASE
(2022)
Article
Pharmacology & Pharmacy
Chih-Feng Lien, Chin-Sheng Lin, Song-Kun Shyue, Po-Shiuan Hsieh, Sy-Jou Chen, Yi-Tan Lin, Shu Chien, Min-Chien Tsai
Summary: The study investigated the pathological roles of PPARd in atherosclerotic plaque vulnerability. The results showed that plasma PPARd levels were lower in patients with ACS than those with SA. PPARd agonist reduced plaque vulnerability, synthetic SMC phenotype, ER stress, and NLRP3 inflammasome expression in ApoE(-/-) mice fed a high-cholesterol diet. PPARd protected against SMC phenotypic switching by inhibiting NF?B signaling and alleviating ER stress.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Yi Song, Tao Wang, Chunjie Mu, Wenting Gui, Yao Deng, Runwei Ma
Summary: This study found that SENCR inhibits vascular smooth muscle cell proliferation, migration, and synthetic phenotype-related gene expression in aortic dissection through the miR-206/myocardin axis, thereby maintaining the contractile phenotype and suppressing aortic dilatation.
NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES
(2022)
Review
Cell Biology
Hao-Yue Tang, Ai-Qun Chen, Huan Zhang, Xiao-Fei Gao, Xiang-Quan Kong, Jun-Jie Zhang
Summary: This article provides an updated summary of the recent studies and systematic knowledge of vascular smooth muscle cells (VSMCs) phenotypic switching in cardiovascular diseases such as atherosclerosis, in-stent restenosis, aortic aneurysms, and vascular calcification. It may guide future research and provide novel insights into the prevention and treatment of related diseases.
Review
Cardiac & Cardiovascular Systems
Runji Chen, David G. McVey, Daifei Shen, Xiaoxin Huang, Shu Ye
Summary: This article discusses the different VSMC phenotypes in atherosclerotic lesions and introduces various methods for studying VSMC phenotypic switching.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Article
Genetics & Heredity
Adam W. Turner, Shengen Shawn Hu, Jose Verdezoto Mosquera, Wei Feng Ma, Chani J. Hodonsky, Doris Wong, Gaelle Auguste, Yipei Song, Katia Sol-Church, Emily Farber, Soumya Kundu, Anshul Kundaje, Nicolas G. Lopez, Lijiang Ma, Saikat Kumar B. Ghosh, Suna Onengut-Gumuscu, Euan A. Ashley, Thomas Quertermous, Aloke Finn, Nicholas J. Leeper, Jason C. Kovacic, Johan L. M. Bjoerkgren, Chongzhi Zang, Clint L. Miller
Summary: This study applied single-nucleus technology to analyze nuclei from patients with coronary artery disease, identifying elements and transcription factors associated with the disease and proposing key driver transcription factors. This research is of great significance for understanding the regulatory mechanisms of coronary artery disease risk.
Article
Biochemistry & Molecular Biology
Yunxiao Yang, Enzehua Xie, Yuhua Liu, Zhan Peng, Cuntao Yu, Kun Hua, Xiubin Yang
Summary: The study found that patients with Marfan syndrome (MFS) have elevated blood calcium levels and dyslipidemia. Increased calcium concentration is associated with promoted vascular smooth muscle cell (VSMC) phenotype switching in MFS. SERCA may become a novel therapeutic target for suppressing aneurysm progression in MFS.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Cansu Karakaya, Mark C. C. van Turnhout, Valery L. L. Visser, Tommaso Ristori, Carlijn V. C. Bouten, Cecilia M. M. Sahlgren, Sandra Loerakker
Summary: Mechanical stimuli and Notch signaling play important roles in regulating vascular growth and remodeling. This study reveals that cyclic strain decreases Notch signaling and leads to the loss of contractile features in vascular smooth muscle cells. Activation of Notch signaling partially rescues the contractile features of these cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Runwei Ma, Dayong Zhang, Yi Song, Jichang Kong, Chunjie Mu, Pin Shen, Wenting Gui
Summary: This study explores the function and mechanism of miR-335-5p in aortic dissection (AD). The results indicate that miR-335-5p inhibits proliferation, migration, and phenotypic switching of vascular smooth muscle cells (VSMCs) by negatively regulating specificity protein 1 (SP1), suggesting that miR-335-5p may be a potential therapeutic target in AD.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2022)
Article
Cell Biology
Ravi Abishek Bharadhwaj, Regalla Kumarswamy
Summary: This study reveals a novel role for the lncRNA TUG1 in modulating SMC differentiation via the KLF4-myocardin axis, which may have potential implications in AAA development.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yu Zhou, Shaligram Sharma, Xiaonan Sun, Xiaoqing Guan, Yuning Hou, Zhe Yang, Hang Shi, Ming-Hui Zou, Ping Song, Jiliang Zhou, Shenming Wang, Zuojun Hu, Chunying Li
Summary: SMYD2 is a histone lysine methyltransferase that regulates carcinogenesis and inflammation. In this study, the role of SMYD2 in VSMC phenotypic modulation and vascular intimal hyperplasia was investigated. The results showed that downregulation of SMYD2 promoted VSMC phenotypic switching and enhanced proliferation and migration. On the other hand, overexpression of SMYD2 inhibited VSMC proliferation and migration, and attenuated arterial narrowing. Mechanistically, SMYD2 regulated VSMC contractile gene expression by promoting the expression and transactivation of myocardin, leading to an open chromatin state around SMC contractile gene promoters via SMYD2-mediated H3K4 methylation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Jing Wang, Xiaoxiang Tian, Chenghui Yan, Hanlin Wu, Yuxin Bu, Jia Li, Dan Liu, Yaling Han
Summary: Phenotypic switching of vascular smooth muscle cells plays a central role in abdominal aortic aneurysm pathology. Knockdown of TCF7L1 inhibits the differentiation of vascular smooth muscle cells. This study suggests potential interventions to prevent AAA by maintaining a normal differentiated smooth muscle cell state, and TCF7L1 may serve as a biomarker for AAA.
JACC-BASIC TO TRANSLATIONAL SCIENCE
(2023)
Article
Hematology
Yun-Fei Yan, Jian-Fei Pei, Yang Zhang, Ran Zhang, Fang Wang, Peng Gao, Zhu-Qin Zhang, Ting-Ting Wang, Zhi-Gang She, Hou-Zao Chen, De-Pei Liu
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2017)
Article
Oncology
Ke Chen, Haibing Xiao, Jin Zeng, Gan Yu, Hui Zhou, Chunhua Huang, Weimin Yao, Wei Xiao, Junhui Hu, Wei Guan, Lily Wu, Jiaoti Huang, Qihong Huang, Hua Xu, Zhangqun Ye
CLINICAL CANCER RESEARCH
(2017)
Article
Biochemistry & Molecular Biology
Siyu Xia, Jing Feng, Ke Chen, Yanbing Ma, Jing Gong, Fangfang Cai, Yuxuan Jin, Yang Gao, Linjian Xia, Hong Chang, Lei Wei, Leng Han, Chunjiang He
NUCLEIC ACIDS RESEARCH
(2018)
Article
Oncology
Tao Yang, Hui Zhou, Peijun Liu, Libin Yan, Weimin Yao, Ke Chen, Jin Zeng, Heng Li, Junhui Hu, Hua Xu, Zhangqun Ye
Article
Biochemistry & Molecular Biology
Jing Feng, Ke Chen, Xin Dong, Xiaolong Xu, Yuxuan Jin, Xinyang Zhang, Wenbo Chen, Yujing Han, Lin Shao, Yang Gao, Chunjiang He
Article
Oncology
Jin Zeng, Wei Xiang, Yucong Zhang, Chunhua Huang, Ke Chen, Zhiqiang Chen
Review
Cell Biology
Pei-Heng Li, Ran Zhang, Li-Qin Cheng, Jin-Jing Liu, Hou-Zao Chen
AGEING RESEARCH REVIEWS
(2020)
Review
Oncology
Gongwei Long, Siquan Ma, Runlin Shi, Yi Sun, Zhiquan Hu, Ke Chen
Summary: This review focuses on the role of circular RNAs (circRNAs) in drug resistance in genitourinary tumors and summarizes the underlying mechanisms. It highlights the essential role of circRNAs in drug resistance development and points out potential future research directions. The potential clinical prospect of circRNAs in regulating tumor drug resistance is also discussed.
Article
Genetics & Heredity
Zhiquan Hu, Siquan Ma, Yi Sun, Gongwei Long, Ke Chen
Summary: The abnormal expression of long non-coding RNAs (lncRNAs) is closely related to the prognosis of patients, which may indicate a new target for the treatment of malignant tumors. The study identified MIR4435-2HG as the only lncRNA related to the prognosis and treatment of BCG-resistant patients and validated its prognostic value in bladder cancer.
Article
Biochemical Research Methods
Joanna Bons, Jacob Rose, Ran Zhang, Jordan B. Burton, Christopher Carrico, Eric Verdin, Birgit Schilling
Summary: Post-translational modifications (PTMs) dynamically regulate proteins and biological pathways through combined effects of multiple PTMs. This study presents a powerful approach combining antibody-based enrichment, comprehensive data-independent acquisition (DIA), and spectral library-free data processing. This novel workflow allows for confident PTM identification, precise site localization, and accurate quantification. The analysis of mouse brains revealed hundreds of malonylated and succinylated peptides and demonstrated the impact of SIRT5 regulation on these PTMs and related pathways.
Article
Multidisciplinary Sciences
Ran Zhang, Joanna Bons, Grace Scheidemantle, Xiaojing Liu, Olga Bielska, Chris Carrico, Jacob Rose, Indra Heckenbach, Morten Scheibye-Knudsen, Birgit Schilling, Eric Verdin
Summary: This study found that the posttranslational modification lysine malonylation is present in many proteins, including histones. The availability of malonyl-CoA affects lysine malonylation, and the deacylase SIRT5 reduces malonylation of histones. Knocking down KAT2A reduced histone malonylation levels. Mass spectrometry revealed that H2B_K5 was highly malonylated and regulated by SIRT5. Additionally, histone malonylation increased nucleolar area and ribosomal RNA expression, and the levels of lysine malonylation and ACC expression were higher in older mouse brains. These experiments highlight the importance of histone malonylation in ribosomal gene expression.
Article
Biology
Wenbo Chen, Xin Chen, Zhenyu Zhao, Menglu Li, Shuang Dong, Sheng Hu, Xiaoyu Li, Mingqian Feng, Ke Chen, Shan Zhong, Chunjiang He
Summary: This study systematically analyzed the relationship between metabolic pathways and prognosis in multiple cancers, and found significant associations between the expression of metabolic checkpoint genes, activity of metabolic pathways, and survival of cancer patients. This provides important clues for further research on cancer diagnosis and therapy.
Article
Multidisciplinary Sciences
Ran Zhang, Xu Wang, Jia-Hua Qu, Bing Liu, Peng Zhang, Tao Zhang, Peng-Cheng Fan, Xiao-Man Wang, Guang-Yuan Xiao, Ye Su, Yan Xie, Yue Liu, Jian-Fei Pei, Zhu-Qin Zhang, De-Long Hao, Ping Xu, Hou-Zao Chen, De-Pei Liu
Article
Biochemical Research Methods
Lijun Lei, Siyu Xia, Dan Liu, Xiaoqing Li, Jing Feng, Yaqi Zhu, Jun Hu, Linjian Xia, Lieping Guo, Fei Chen, Hui Cheng, Ke Chen, Hanyang Hu, Xiaohua Chen, Feng Li, Shan Zhong, Nupur Mittal, Guohua Yang, Zhijian Qian, Leng Han, Chunjiang He
BRIEFINGS IN BIOINFORMATICS
(2018)
Review
Urology & Nephrology
Heng Li, Zhize Wang, Kun Tang, Hui Zhou, Haoran Liu, Libin Yan, Wei Guan, Ke Chen, Hua Xu, Zhangqun Ye
EUROPEAN UROLOGY FOCUS
(2018)