Article
Pharmacology & Pharmacy
Hua Chai, Hua Qu, Shan He, Lei Song, Yu Yang, Hongbo Huang, Dazhuo Shi
Summary: Studies have found that zedoarondiol improves atherosclerosis plaques by inhibiting monocyte migration and adhesion to endothelial cells, and regulating the CXCL12/CXCR4 pathway. This suggests that zedoarondiol may be a promising new drug for atherosclerosis.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Elena Madrazo, Raquel Gonzalez-Novo, Candido Ortiz-Placin, Mario Garcia de Lacoba, Africa Gonzalez-Murillo, Manuel Ramirez, Javier Redondo-Munoz
Summary: CXCL12 has novel regulatory functions in nuclear deformability and epigenetic changes of T-ALL cells, making it a potential pharmacological target for treating T-ALL dissemination.
Article
Oncology
Nicholas R. Anderson, Vipul Sheth, Hui Li, Mason W. Harris, Shaowei Qiu, David K. Crossman, Harish Kumar, Puneet Agarwal, Takashi Nagasawa, Andrew J. Paterson, Robert S. Welner, Ravi Bhatia
Summary: Using a mouse model of FLT3-ITD AML, researchers found that FLT3-ITD AML LSC were enriched within the ST-HSC population and had increased expression of CXCR4. Deletion of CXCL12 from the microenvironment enhanced targeting of AML cells by Flt3-TKI plus chemotherapy treatment, including enhanced LSC targeting. Inhibition of p38 signaling reduced the maintenance of Flt3-ITD AML LSC and enhanced their sensitivity to treatment.
Article
Chemistry, Multidisciplinary
Camelia-Mihaela Zara-Danceanu, Anca-Emanuela Minuti, Cristina Stavila, Luminita Labusca, Dumitru-Daniel Herea, Crina Elena Tiron, Horia Chiriac, Nicoleta Lupu
Summary: Magnetic nanoparticles (MNPs) have potential applications in drug delivery, tracking, and targeting agents in the biomedical field. Coating MNPs with lipids can improve their stability and dispersibility, and enhance cell viability. The choice of coating and cell type can affect the senescence resistance and mobility of loaded cells. Coating MNPs with oleic acid (OA) has shown significant improvements in cell activity and mobility.
Article
Cell Biology
Liam A. Ridge, Dania Kewbank, Dagmar Schuetz, Ralf Stumm, Peter J. Scambler, Sarah Ivins
Summary: The study shows that CXCL12 signaling through its receptor CXCR4 plays distinct roles in different stages of SLV development, guiding cellular distribution and regulating cell proliferation. The atypical chemokine receptor CXCR7 may have a role in regulating ligand bioavailability during SLV morphogenesis.
Article
Biochemistry & Molecular Biology
Linhao Zhang, Chong Zhao, Wenting Dai, Huan Tong, Wenjuan Yang, Zhiyin Huang, Chengwei Tang, Jinhang Gao
Summary: Disrupting the interaction between cholangiocytes and B cells can attenuate liver fibrosis by blocking the CXCL12-CXCR4 axis and utilizing the COX-2 independent effect of celecoxib.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Hematology
Bojing Shao, Christopher Hoover, Huiping Shi, Yuji Kondo, Robert H. Lee, Junmei Chen, Xindi Shan, Jianhua Song, J. Michael McDaniel, Meixiang Zhou, Samuel McGee, Karen Vanhoorelbeke, Wolfgang Bergmeier, Jose A. Lopez, James N. George, Lijun Xia
Summary: GPIba-mediated activation of integrin alpha IIb beta 3 plays an important role in the formation of thrombosis in patients with thrombotic thrombocytopenic purpura (TTP).
Article
Biochemistry & Molecular Biology
Diana Romenskaja, Karolina Kriauciunaite, Justina Pajarskiene, Vytautas Kaseta, Virginijus Tunaitis, Tarja Malm, Rashid Giniatulin, Augustas Pivoriunas
Summary: This study found that EVs derived from human exfoliated deciduous teeth stem cells can promote microglial motility and migration through the P2X4R/MFG-E8 dependent mechanism. These findings provide new insights into the molecular mechanisms through which EVs target human microglia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Yoshimi Oka
Summary: Membrane-anchored proteins and their mimics, such as PNAs, show selective partitioning into lipid raft or non-raft domains based on their lipophilic anchors. In this study, anchor-matched PNA was found to be excluded from the lipid raft domains of giant unilamellar vesicles when hybridized with complementary flavin-labeled DNA. The distribution of PNA was not only dependent on the lipophilic anchor, but also on the structure of the hybridized DNA or PNA pair.
Article
Cell Biology
Jiachi Ma, Wanqing Liang, Yaosheng Qiang, Lei Li, Jun Du, Chengwu Pan, Bangling Chen, Chensong Zhang, Yuzhong Chen, Qingkang Wang
Summary: The study investigated the cooperative role of CXCR4/CXCL12 axis and IL-1Ra in the metastasis of colorectal cancer cells. It was found that IL-1 alpha and rIL-1 alpha promoted CXCL12 expression, while IL-1Ra inhibited this effect. Additionally, CXCL12 enhanced migration and proliferation of endothelial cells, and IL-1Ra inhibited migration, proliferation, and angiogenesis of colorectal cancer cells.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Pharmacology & Pharmacy
Ting Xiao, Dandi Gao, Xiaoting Gu, Yanping Zhang, Yuxin Zhu, Zihui Zhang, Yiming He, Luqing Wei, Hongli Li, Honggang Zhou, Cheng Yang
Summary: This study evaluated the effect of Flavokawain A (FKA) on pulmonary fibrosis and found that FKA alleviated the development of fibrosis and improved lung function by inhibiting TGF-beta 1/Smad signaling and myofibroblast activation. Additionally, FKA reduced extracellular matrix production and collagen deposition by blocking CXCL12/CXCR4 signaling and inhibiting CXCL12 dimerization. FKA shows promise as a therapeutic agent for pulmonary fibrosis.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Environmental Sciences
Nikita Gupta, Chakrabhavi Dhananjaya Mohan, Muthu K. Shanmugam, Young Yun Jung, Arunachalam Chinnathambi, Sulaiman Ali Alharbi, Milad Ashrafizadeh, Manas Mahale, Andreas Bender, Alan Prem Kumar, Thomas Choudary Putti, Kanchugarakoppal S. Rangappa, Xianbin Zhang, Kwang Seok Ahn, Gautam Sethi
Summary: Environmental factors and lack of therapeutic targets contribute to the development and poor prognosis of TNBC. This study identified CXCR4 as a potential therapeutic target in TNBC, and found that ZGA can downregulate CXCR4 expression and inhibit TNBC cell migration and invasion. Furthermore, ZGA was effective in inhibiting tumor growth and metastasis in a TNBC mouse model. Overall, this research highlights the importance of targeting CXCR4 in the treatment of TNBC.
ENVIRONMENTAL RESEARCH
(2023)
Article
Oncology
Xun Zheng, Shu Rui, Xiao-Fei Wang, Xiu-He Zou, Yan-Ping Gong, Zhi-Hui Li
Summary: The study shows that miR-455-5p suppresses MTC growth and metastasis by targeting the CXCL12/CXCR4 signaling pathway, while circPVT1 promotes MTC growth and metastasis.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Kaoru Kurowarabe, Masataka Endo, Daichi Kobayashi, Haruko Hayasaka
Summary: Lymphocytes stimulated by CXCL12 can affect surrounding cells' chemotactic response, promoting chemotaxis of CXCR4-deficient cells but inhibiting CCR7 ligand-dependent directionality. This suggests that paracrine factors can guide surrounding cells to migrate towards CXCL12 by regulating responsiveness to chemokines.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2021)
Article
Chemistry, Analytical
Minggang Tian, Yaru Sun, Xiuqi Kong, Baoli Dong
Summary: Studying the phase separation of ER membranes is crucial for understanding the fundamental biology of ER. However, NIR ratiometric imaging of ER phase behaviors has not been explored in living cells and diverse tissues. In this study, we developed a polarity-responsive NIR fluorescent probe to visualize the phase behavior in ER membranes and revealed different phase behaviors in different tissues.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yuka Maetani, Satoshi Asano, Akiko Mizokami, Yosuke Yamawaki, Tomomi Sano, Masato Hirata, Masahiro Irifune, Takashi Kanematsu
Summary: The study reveals that PRIP regulates cell cycle progression by modulating the PI3K/AKT signaling pathway, influencing proliferation and tumor growth of breast cancer cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Pharmacology & Pharmacy
Masaya Matsuda, Yoshiyuki Tanaka, Hayato Shimora, Naoki Takemoto, Miku Nomura, Ryogo Terakawa, Kennosuke Hashimoto, Harumi Sakae, Akira Kanda, Hiroshi Iwai, Kazuyuki Kitatani, Takeshi Nabe
Summary: This study aimed to develop a murine model of steroid-resistant asthma and investigate the role of group 2 innate lymphoid cells (ILC2s) in producing IL-5, a type-2 cytokine. The results showed that in steroid-resistant asthma, ILC2s produced larger amounts of IL-5 in response to IL-33/TSLP, and the up regulation of TSLP receptors on ILC2s may contribute to the pathogenic alteration. Treatment with anti-IL-5 monoclonal antibody in combination with dexamethasone effectively suppressed airway remodeling in the steroid-resistant model. Overall, IL-5 blockage could be a promising therapeutic strategy for steroid-resistant asthma.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Keiko Tamiya-Koizumi, Yurika Otoki, Kiyotaka Nakagawa, Reiji Kannagi, Naoki Mizutani, Motoshi Suzuki, Mamoru Kyogashima, Soichiro Iwaki, Mineyoshi Aoyama, Takashi Murate, Kazuyuki Kitatani, Takahisa Kuga, Yasuyoshi Mizutani, Akira Tokumura
Summary: Plasmalogen, localized in the raft of mammalian cell membranes, is involved in the storage of polyunsaturated fatty acids (PUFA) and is found to be more abundant in malignant cells surviving and growing in hypoxic conditions. In this study using liquid chromatography-tandem mass spectrometry, it was observed that the intracellular concentration of plasmalogen species containing a PUFA at the sn-2-position did not change for two days under hypoxic culture in human colorectal cancer-derived Caco2 cells. However, on the third day of hypoxia, there was a significant increase in ethanolamine plasmalogen and choline plasmalogen compared to the second day. This increase was suggested to be regulated by the up-regulation of mRNA of Ca2+-independent phospholipase A(2)beta and cytoplasmic phospholipase A(2)gamma, as well as the down-regulation of lysoplasmalogenase in Caco2 cells under hypoxia.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Jun Fujita, Makoto Taniguchi, Chieko Hashizume, Yoshibumi Ueda, Shota Sakai, Tadakazu Kondo, Mayumi Hashimoto-Nishimura, Kentaro Hanada, Takeo Kosaka, Toshiro Okazaki
Summary: Ceramide plays a role in NCS-induced apoptosis by enhancing the assembly of ATM and MRN complex proteins. NCS increases ceramide production through the activation of nSMase and suppression of SMS, leading to the colocalization of ceramide with ATM and MRN complex. Inhibition of ceramide generation and nSMase activity can prevent the activation of ATM and apoptosis induced by NCS.
MOLECULAR PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Satoshi Asano, Yuka Maetani, Yukio Ago, Takashi Kanematsu
Summary: In this study, we found that phospholipase C-related catalytically inactive protein (PRIP) enhances cisplatin-induced apoptosis in breast cancer cells. PRIP promotes XIAP degradation by inhibiting PI (3,4,5)P3/AKT signaling, leading to enhanced cisplatin-induced apoptotic cell death. Therefore, PRIP1-PH liposomes may be a novel agent to avoid cisplatin resistance.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Immunology
Masaya Matsuda, Miki Inaba, Junpei Hamaguchi, Hiro Tomita, Miyu Omori, Hayato Shimora, Harumi Sakae, Kazuyuki Kitatani, Takeshi Nabe
Summary: IL-10 can suppress eosinophil and neutrophil infiltration by inhibiting the proliferation of ICAM-1+ and VCAM1+ pulmonary vascular endothelial cells, resulting in inhibition of airway hyperresponsiveness in steroid-insensitive asthmatic mice.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Makoto Taniguchi, Shingo Nagaya, Kohei Yuyama, Ai Kotani, Yasuyuki Igarashi, Toshiro Okazaki
Summary: Ceramide metabolism through receptor-mediated exosome release plays a crucial role in drug resistance and cell proliferation in leukemia cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Eiji Imado, Samnang Sun, Abrar Rizal Abawa, Takeru Tahara, Takahiro Kochi, Tran Ngoc Bao Huynh, Satoshi Asano, Shigeru Hasebe, Yoki Nakamura, Kazue Hisaoka-Nakashima, Yaichiro Kotake, Masahiro Irifune, Kazuhiro Tsuga, Kazuhiro Takuma, Norimitsu Morioka, Norikazu Kiguchi, Yukio Ago
Summary: Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by deficits in social communication and social interaction and the presence of restricted, repetitive behaviors. This study found that prenatal exposure to valproic acid (VPA) can cause increased tactile sensitivity and that spinal microglia play an important role in regulating nociceptive responses.
NEUROCHEMISTRY INTERNATIONAL
(2022)
Article
Neurosciences
Yukio Ago, Rei Yokoyama, Satoshi Asano, Hitoshi Hashimoto
Summary: The antidepressant effects of ketamine may be related to both the dopaminergic system and the serotonergic system, while the effects of (R)-ketamine may be independent of the monoaminergic system. Ketamine metabolites differ in their ability to regulate monoamine neurotransmitters, and some metabolites may share common serotonergic signaling mechanisms with ketamine.
Article
Oncology
Satoshi Asano, Misa Yamasaka, Kairi Ozasa, Kotaro Sakamoto, Atsuko Hayata-Takano, Takanobu Nakazawa, Hitoshi Hashimoto, James A. A. Waschek, Yukio Ago
Summary: Phosphoinositide metabolism plays a critical role in human cancer cell migration and metastatic growth. This study demonstrates that VIPR2 regulates WAVE2-mediated actin nucleation and elongation for lamellipodium formation through the synthesis of PI(3,4,5)P-3, thereby controlling cancer cell migration.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yosuke Yamawaki, Hiroki So, Kana Oue, Satoshi Asano, Hisako Furusho, Mutsumi Miyauchi, Kotaro Tanimoto, Takashi Kanematsu
Summary: Periodontal disease is associated with neural diseases, and Porphyromonas gingivalis induces brain inflammation and neural damage through inflammatory responses. The antidepressant imipramine inhibits inflammatory responses and ameliorates neural damage related to periodontal disease.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Kotaro Sakamoto, Satoshi Asano, Yukio Ago, Takatsugu Hirokawa
Summary: In this study, the binding mechanism between VIPR2 and KS-133 was elucidated through the construction of models and simulations. The study also discovered the functional significance of the S-S bond formation between Cys residues in VIPR2.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Microbiology
Masaya Matsuda, Seito Shimizu, Kazuyuki Kitatani, Takeshi Nabe
Summary: Extracellular vesicles play a crucial role in allergen immunotherapy by transforming into a suppressive phenotype of type 2 allergic inflammation.
Article
Biochemistry & Molecular Biology
Satoshi Asano, Ami Ono, Kotaro Sakamoto, Atsuko Hayata-Takano, Takanobu Nakazawa, Kotaro Tanimoto, Hitoshi Hashimoto, Yukio Ago
Summary: VIPR2 overexpression promotes breast cancer cell proliferation and tumor growth by activating the cAMP/PKA/ERK signaling pathway.
Article
Cell Biology
Harumi Sakae, Yuri Ogiso, Masaya Matsuda, Hayato Shimora, Tye Deering, Todd E. Fox, Mark Kester, Takeshi Nabe, Kazuyuki Kitatani
Summary: This study investigated the therapeutic effects of ceramide nanoliposomes (CNL) on type 2 inflammation-based asthma. The results showed that CNL treatment significantly decreased airway remodeling and suppressed goblet cell hyperplasia induced by ovalbumin challenges. This suggests that CNL may have therapeutic effects on airway diseases like asthma.
