期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 31, 期 22, 页码 4550-4562出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.05817-11
关键词
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资金
- Academy of Sciences of the Czech Republic [AV0Z50520514]
- GACR [MEM/09/E011]
- Center of Molecular and Cellular Immunology (Ministry of Education, Youth and Sports of the Czech Republic) [1M0506]
- Faculty of Science, Charles University, Prague, Czech Republic
Formation of the immunological synapse between an antigen-presenting cell (APC) and a T cell leads to signal generation in both cells involved. In T cells, the lipid raft-associated transmembrane adaptor protein LAT plays a central role. Its phosphorylation is a crucial step in signal propagation, including the calcium response and mitogen-activated protein kinase activation, and largely depends on its association with the SLP76 adaptor protein. Here we report the discovery of a new palmitoylated transmembrane adaptor protein, termed SCIMP. SCIMP is expressed in B cells and other professional APCs and is localized in the immunological synapse due to its association with tetraspanin-enriched microdomains. In B cells, it is constitutively associated with Lyn kinase and becomes tyrosine phosphorylated after major histocompatibility complex type II (MHC-II) stimulation. When phosphorylated, SCIMP binds to the SLP65 adaptor protein and also to the inhibitory kinase Csk. While the association with SLP65 initiates the downstream signaling cascades, Csk binding functions as a negative regulatory loop. The results suggest that SCIMP is involved in signal transduction after MHC-II stimulation and therefore serves as a regulator of antigen presentation and other APC functions.
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