Article
Multidisciplinary Sciences
Kensuke Toriumi, Yuta Onodera, Toshiyuki Takehara, Tatsufumi Mori, Joe Hasei, Kanae Shigi, Natsumi Iwawaki, Toshifumi Ozaki, Masao Akagi, Mahito Nakanishi, Takeshi Teramura
Summary: In this study, the researchers discovered that the transmembrane protein LRRC15 correlated with the expression of TWIST1 in MSCs, and could be used to estimate the stemness of MSCs. The LRRC15-positive MSC populations expressed stemness-associated transcription factors and therapeutic cytokines, and showed enhanced therapeutic effects in a pulmonary fibrosis mouse model. This study highlights the importance of TWIST1 in MSC stemness and the utility of LRRC15 as a marker for evaluating stem cell quality in MSCs before transplantation.
Article
Cell Biology
Haibin Jiang, Mingxia Du, Yaning Li, Tengfei Zhou, Jia Lei, Hongqing Liang, Zhen Zhong, Rafia S. Al-Lamki, Ming Jiang, Jun Yang
Summary: It has been found that ID1 and ID3 play important roles in the survival and pluripotency of hESCs, regulating the transition between primed and naive-like states through the AKT signaling pathway. TCF3 mediates transcriptional inhibition of MCL1 to promote AKT phosphorylation.
CELL DEATH & DISEASE
(2022)
Article
Toxicology
Chun-Hong Yu, Shui-Qing Yang, Yu-Jing Zhang, Long Rong, Zong-Chun Yi
Summary: This study investigated the role of GATA factors in hydroquinone (HQ) inhibited erythroid differentiation in K562 cells. The results showed that HQ reduced the occupancy of GATA1 and increased the expression of GATA2, thereby inhibiting erythroid differentiation.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Multidisciplinary Sciences
Monika Gonczi, Joao M. C. Teixeira, Susana Barrera-Vilarmau, Laura Mediani, Francesco Antoniani, Tamas Milan Nagy, Krisztina Feher, Zsolt Raduly, Viktor Ambrus, Jozsef Tozser, Endre Barta, Katalin E. Kover, Laszlo Csernoch, Serena Carra, Monika Fuxreiter
Summary: The context-dependent higher-order assembly of Mef2D controls muscle lineage development by simultaneously interacting with the basal machinery and myogenic factors. The study found that the alternatively spliced acidic beta-domain enhances the transcription of Mef2D. The beta-domain can serve as an interaction element for higher-order assembly, forming nuclear condensates and solid-like aggregates. These findings suggest that the beta-domain fine-tunes Mef2D assembly and provides a platform for myogenic regulatory factors and transcriptional apparatus during muscle development.
NATURE COMMUNICATIONS
(2023)
Article
Cell & Tissue Engineering
Chuti Laowtammathron, Chanchao Lorthongpanich, Nittaya Jiamvoraphong, Pimonwan Srisook, Phatchanat Klaihmon, Pakpoom Kheolamai, Sudjit Luanpitpong, Surapol Issaragrisil
Summary: The study explores the role of YAP in hematopoietic differentiation of hiPSCs. It is found that suppressing YAP activity enhances the efficiency of hematopoietic differentiation and increases the yield of HSPCs.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Genetics & Heredity
Anastasia Ryzhkova, Nariman Battulin
Summary: Hematopoiesis serves as a convenient model to study how chromatin dynamics regulate cell fate. The interaction between transcription factors and epigenetic modifiers leads to silencing of multipotent transcriptome and activation of transcriptional program for terminal differentiation.
Article
Cell Biology
Jing Xu, Fengtian Li, Ya Gao, Rongtian Guo, Liangping Ding, Mengyuan Fu, Yong Yi, Hu Chen, Zhi-Xiong Jim Xiao, Mengmeng Niu
Summary: Targeted therapy has significantly improved survival and prognosis of cancer patients, but treatment progress for squamous cell carcinoma (SCC) lags behind that for adenocarcinoma. The transcription factor E47 plays different roles in cell proliferation in adenocarcinoma and SCC, acting as a tumor suppressor in the former and an oncogene in the latter. The E47-Delta Np63 alpha axis may serve as potential therapeutic targets for squamous cell carcinoma.
CELL DEATH & DISEASE
(2021)
Article
Multidisciplinary Sciences
Abhinav Adhikari, William Kim, Judith Davie
Summary: The skeletal muscle gene expression is regulated by the myogenic regulatory family (MRF) with key factors MyoD and myogenin (MYOG). MYOG is essential for recruiting TBP and RNAPII to muscle gene promoters, while MyoD1 and MYOG act in a feed forward loop to maintain each other's expression. The study demonstrates that MYOG, not MYOD1, is required to activate gene expression on late muscle gene promoters bound by both factors.
Review
Medicine, General & Internal
Angela Quispe-Salcedo, Hayato Ohshima
Summary: The dental pulp is a vital connective tissue responsible for maintaining tooth vitality after injuries. This literature review focuses on the role of dendritic cells in dentinogenesis and proposes the concept of dentin-pulp immunology for understanding the interactions among different cell types in the pulp tissue.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Pharmacology & Pharmacy
Tomasz M. Grzywa, Dominika Nowis, Jakub Golab
Summary: CECs play diverse roles in immune response, from affecting vulnerability to infectious diseases in neonates, regulating feto-matemal tolerance in pregnant women, to influencing immune response in patients with cancer. Their mechanisms include suppressing proinflammatory responses, decreasing T-cell proliferation and promoting T-cell differentiation into regulatory T-cells.
PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Stacia L. Koppenhafer, Kelli L. Goss, Ellen Voigt, Emma Croushore, William W. Terry, Jason Ostergaard, Peter M. Gordon, David J. Gordon
Summary: Sarcomas are difficult to treat and have long-term and life-threatening side effects. The treatment for many sarcomas has not changed much over the past two decades. However, a study found that targeting the ID family of proteins with the FDA-approved drug homoharringtonine may provide a novel therapeutic approach for Ewing sarcoma tumors.
Article
Biochemistry & Molecular Biology
Chie Suzuki, Tohru Fujiwara, Hiroki Shima, Koya Ono, Kei Saito, Hiroki Kato, Koichi Onodera, Satoshi Ichikawa, Noriko Fukuhara, Yasushi Onishi, Hisayuki Yokoyama, Yukio Nakamura, Kazuhiko Igarashi, Hideo Harigae
Summary: The study revealed that FAM210B protein plays a crucial role in erythroid differentiation by regulating mitochondrial energy metabolism, providing new insights into the mechanism of erythroid differentiation.
MOLECULAR AND CELLULAR BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Kai P. Hoefig, Alexander Reim, Christian Gallus, Elaine H. Wong, Gesine Behrens, Christine Conrad, Meng Xu, Lisa Kifinger, Taku Ito-Kureha, Kyra A. Y. Defourny, Arie Geerlof, Josef Mautner, Stefanie M. Hauck, Dirk Baumjohann, Regina Feederle, Matthias Mann, Michael Wierer, Elke Glasmacher, Vigo Heissmeyer
Summary: Post-transcriptional gene regulation in T cells is a dynamic and complex process, involving multiple RNA-binding proteins and unexpected functions for certain transcription factors and signal transducers. By studying the regulation of Roquin-1 and Roquin-2, it was found that multiple RBPs contribute to the coregulation of mRNA targets by individual trans-acting factors.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Tomasz M. Grzywa, Anna Sosnowska, Zuzanna Rydzynska, Michal Lazniewski, Dariusz Plewczynski, Klaudia Klicka, Milena Malecka-Gieldowska, Anna Rodziewicz-Lurzynska, Olga Ciepiela, Magdalena Justyniarska, Paulina Pomper, Marcin M. Grzybowski, Roman Blaszczyk, Michal Wegrzynowicz, Agnieszka Tomaszewska, Grzegorz Basak, Jakub Golab, Dominika Nowis
Summary: The study shows that early CD71+ erythroid cells expand in the spleen of anemic mice and humans, utilizing high levels of arginase 2 (ARG2) and reactive oxygen species (ROS) to suppress T-cell activation. These findings provide insight into the regulatory role of CECs in the immune response.
COMMUNICATIONS BIOLOGY
(2021)
Article
Cell Biology
Marc Garcia-Montolio, Cecilia Ballare, Enrique Blanco, Arantxa Gutierrez, Sergi Aranda, Antonio Gomez, Chung H. Kok, David T. Yeung, Timothy P. Hughes, Pedro Vizan, Luciano Di Croce
Summary: Polycomb group proteins, including PHF19, play important roles in regulating cell fate determination and could be potential therapeutic targets for myeloid leukemia treatment. PHF19 depletion reduces cell proliferation and promotes leukemia cell differentiation, with its homolog MTF2 partially compensating for its effects in specific gene targets.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Janis V. de la Iglesia, Robbert J. C. Slebos, Laura Martin-Gomez, Xuefeng Wang, Jamie K. Teer, Aik Choon Tan, Travis A. Gerke, Garrick Aden-Buie, Tessa van Veen, Jude Masannat, Ritu Chaudhary, Feifei Song, Michelle Fournier, Erin M. Siegel, Matthew B. Schabath, J. Trad Wadsworth, Jimmy Caudell, Louis Harrison, Bruce M. Wenig, Jose Conejo-Garcia, Juan C. Hernandez-Prera, Christine H. Chung
CLINICAL CANCER RESEARCH
(2020)
Article
Hematology
Cristina Fugazza, Gloria Barbarani, Sudharshan Elangovan, Maria Giuseppina Marini, Serena Giolitto, Isaura Font-Monclus, Maria Franca Marongiu, Laura Manunza, John Strouboulis, Claudio Cantu, Fabio Gasparri, Silvia M. L. Barabino, Yukio Nakamura, Sergio Ottolenghi, Paolo Moi, Antonella Ellena Ronchi
Summary: Coup-TFII is identified as a specific activator of the gamma-globin gene, playing a role in activating gamma-globin expression during early embryonic/fetal stages. Overexpression of Coup-TFII can overcome the repression of gamma-globin in adult cells, while knockout of Coup-TFII increases the beta/gamma+beta globin ratio.
Editorial Material
Hematology
John Strouboulis
Article
Hematology
Gaetan Juban, Nathalie Sakakini, Hedia Chagraoui, David Cruz Hernandez, Qian Cheng, Kelly Soady, Bilyana Stoilova, Catherine Garnett, Dominic Waithe, Georg Otto, Jessica Doondeea, Batchimeg Usukhbayar, Elena Karkoulia, Maria Alexiou, John Strouboulis, Edward Morrissey, Irene Roberts, Catherine Porcher, Paresh Vyas
Summary: GATA1s induces defects in erythroid and megakaryocytic differentiation at specific stages in the hematopoietic cellular hierarchy, resulting in a transient myeloproliferative disorder. The effects are more profound in ESC-derived cultures, where a delay in maturation and aberrant gene expression are observed in both erythroid and megakaryocytic lineage cells. Further studies are needed to understand the oncogenic action of GATA1s in the specific hematopoietic compartment.
Article
Physiology
Aishwarya Gurumurthy, Qiong Wu, Rukiye Nar, Kimberly Paulsen, Alexis Trumbull, Ryan C. Fishman, Marjorie Brand, John Strouboulis, Zhijian Qian, Jorg Bungert
FRONTIERS IN PHYSIOLOGY
(2020)
Review
Cell Biology
Alba Saenz de Villaverde Cortabarria, Laura Makhoul, John Strouboulis, Giovanna Lombardi, Eugene Oteng-Ntim, Panicos Shangaris
Summary: Sickle Cell Disease is a prevalent severe monogenic disorder caused by a beta-globin gene missense mutation. Haematopoietic stem cell transplantation is the only curative option, with prenatal diagnosis and fetal therapeutic intervention offering potential in utero treatment. In utero stem cell transplantation shows promise as a non-myeloablative, non-immunosuppressive alternative with unique advantages and potential for safer postnatal management.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Christine H. Chung, Marcelo Bonomi, Conor E. Steuer, Jiannong Li, Priyanka Bhateja, Matthew Johnson, Jude Masannat, Feifei Song, Juan C. Hernandez-Prera, Bruce M. Wenig, Helen Molina, Joaquim M. Farinhas, Caitlin P. McMullen, J. Trad Wadsworth, Krupal B. Patel, Julie A. Kish, Jameel Muzaffar, Kedar Kirtane, James W. Rocco, Michael J. Schell, Nabil F. Saba
Summary: The combination of cetuximab and nivolumab was well tolerated in patients with incurable head and neck squamous cell carcinoma. While patients without prior immune checkpoint inhibitor exposure showed a trend for more favorable progression-free survival, the improvement in 1-year overall survival did not reach statistical significance in this heavily treated patient population. Optimal sequencing of cetuximab and checkpoint inhibitors may impact prognosis and requires further evaluation.
Review
Biochemistry & Molecular Biology
Niko Linzer, Alexis Trumbull, Rukiye Nar, Matthew D. Gibbons, David T. Yu, John Strouboulis, Joerg Bungert
Summary: TFII-I, a key transcription factor, plays crucial roles in regulating Pol II transcription at the stages of initiation and elongation by interacting with various co-regulators and influencing transcription positively or negatively. Its expression changes are associated with a range of diseases and developmental processes, highlighting its important role in gene regulation.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Bowen Yan, Jennifer Yang, Min Young Kim, Huacheng Luo, Nicholas Cesari, Tao Yang, John Strouboulis, Jiwang Zhang, Ross Hardison, Suming Huang, Yi Qiu
Summary: The activity of hematopoietic factor GATA-1 is modulated by acetylation and deacetylation processes mediated by HDAC1, allowing for chromatin binding and transcriptional regulation of erythroid/megakaryocyte commitment and differentiation.
NUCLEIC ACIDS RESEARCH
(2021)
Letter
Hematology
John Brewin, Sara El Hoss, John Strouboulis, David Rees
Editorial Material
Hematology
John Strouboulis, Antonella E. Ronchi
Summary: In this article, Ludwig et al characterize a rare form of inherited hemolytic anemia and identify novel mutations in an intrinsically disordered region (IDR) in the GATA1 carboxyl (C)-terminal domain. These mutations have novel functions in selectively regulating GATA1 chromatin occupancies in erythropoiesis.
Article
Oncology
Christine H. Chung, Jiannong Li, Conor E. Steuer, Priyanka Bhateja, Matthew Johnson, Jude Masannat, Maria I. Poole, Feifei Song, Juan C. Hernandez-Prera, Helen Molina, Bruce M. Wenig, Sunil Kumar, Charlotte Kuperwasser, Philip J. Stephens, Joaquim M. Farinhas, Dong M. Shin, Julie A. Kish, Jameel Muzaffar, Kedar Kirtane, James W. Rocco, Michael J. Schell, Nabil F. Saba, Marcelo Bonomi
Summary: A phase II multi-institutional clinical trial was conducted to evaluate the overall survival (OS) of patients with recurrent and/or metastatic head and neck squamous cell carcinoma (HNSCC) treated with a combination of cetuximab and nivolumab. The results showed that this combination therapy was effective in both previously treated and untreated patients, and the expression of p16 and PD-L1 was associated with treatment response and survival.
CLINICAL CANCER RESEARCH
(2022)
Article
Hematology
John N. Brewin, Amina Nardo-Marino, Sara Stuart-Smith, Sara El Hoss, Anke Hanneman, John Strouboulis, Stephan Menzel, John S. Gibson, David C. Rees
Summary: This study found that alpha-thalassemia significantly reduces erythrocyte KCC activity in both HbSS and HbSC patients, potentially inducing a milder phenotype by reducing red cell cation loss. Additionally, alpha-thalassemia can increase hemoglobin and decrease erythropoietin levels in patients with HbSS, demonstrating a measurable response to improved tissue oxygenation.
AMERICAN JOURNAL OF HEMATOLOGY
(2022)
Editorial Material
Hematology
John Strouboulis, Sara El Hoss
Review
Biotechnology & Applied Microbiology
Claudio Mussolino, John Strouboulis
Summary: Tissue oxygenation depends on the activity of hemoglobin, mutations or dysregulation of which can lead to hemoglobinopathies. Advances in understanding the complex mechanisms controlling globin gene expression and protective genetic traits have led to the exploration of innovative therapeutic approaches for hemoglobinopathies.
FRONTIERS IN GENOME EDITING
(2021)
