Article
Biochemical Research Methods
Christine R. Zheng, Abhyudai Singh, Alexandra Libby, Pamela A. Silver, Elizabeth A. Libby
Summary: By developing modular, direct, and specific sensors of bacterial kinase activity, researchers successfully measured the activity of PrkC kinase in different growth conditions, and found that its activity increased in response to a cell-wall active antibiotic.
ACS SYNTHETIC BIOLOGY
(2021)
Editorial Material
Genetics & Heredity
Hartwig Visser, Adam D. Thomas
Summary: DNA damage-induced miRNAs are important in the DNA damage response, influencing cell fate. However, it is still unclear who is in charge in this process.
TRENDS IN GENETICS
(2021)
Article
Oncology
Xiuyuan Zhang, Qijian Zheng, Xiuying Yue, Zhanna Yuan, Jiming Ling, Yanzhi Yuan, Yanying Liang, Aihua Sun, Yuchen Liu, Hui Li, Kaikun Xu, Fuchu He, Jian Wang, Jin Wu, Chunling Zhao, Chunyan Tian
Summary: This study reveals that ZNF498 is highly expressed in hepatocellular carcinoma (HCC) and is associated with advanced pathological grade and poor prognosis. ZNF498 promotes HCC initiation and progression by inhibiting p53 transcriptional activation and phosphorylation. ZNF498 also inhibits p53-mediated apoptosis and ferroptosis.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Microbiology
Dehui Qu, Xiaohui Zhao, Yao Sun, Fan-Lin Wu, Sheng-Ce Tao
Summary: This study revealed the important role of serine/threonine protein kinase PknB in regulating cell wall formation through phosphorylating the L-rhamnose biosynthesis enzyme RmlA in Mycobacterium tuberculosis. Mutations in RmlA led to negative effects on cell wall formation and reduced virulence in a macrophage model, highlighting the significance of PknB-dependent RmlA phosphorylation in pathogenicity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yawen Gao, Hongsu Wang, Xuening Li, Xiaodi Niu
Summary: Through virtual screening and in vitro bioactivity assays, (-)-epicatechin gallate (ECG), a polyphenol compound from green tea, was found to exhibit significant anti-Ser/Thr phosphatase (Stp1) activity against Staphylococcus aureus (S. aureus). ECG targeted S. aureus Stp1, preventing virulence gene up-regulation and antibody membrane formation, and protected mice from S. aureus infection. Molecular dynamics simulation revealed the allosteric inhibitory mechanism of ECG on Stp1.
Article
Biochemical Research Methods
Transito Garcia-Garcia, Thibaut Douche, Quentin Giai Gianetto, Sandrine Poncet, Nesrine El Omrani, Wiep Klaas Smits, Elodie Cuenot, Mariette Matondo, Isabelle Martin-Verstraete
Summary: This study provides a detailed map of kinase-substrate relationships in C. difficile, revealing the significant role of protein phosphorylation in bacterial cell signaling and pathogenicity. The identified phosphoproteins targeted different pathways involved in bacterial growth and metabolism, shedding light on their regulatory mechanisms.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Microbiology
Transito Garcia-Garcia, Sandrine Poncet, Elodie Cuenot, Thibaut Douche, Quentin Giai Gianetto, Johann Peltier, Pascal Courtin, Marie-Pierre Chapot-Chartier, Mariette Matondo, Bruno Dupuy, Thomas Candela, Isabelle Martin-Verstraete
Summary: The STK signaling pathway regulates PGH homeostasis to precisely control PG hydrolysis during cell division. The study on PG hydrolase CwlA in C. difficile reveals its important role in cell division and its regulation by PrkC, highlighting a novel regulatory mechanism by which STK controls protein export.
Article
Cell Biology
Pengyi Yan, Zixuan Li, Junhao Xiong, Zilong Geng, Weiting Wei, Yan Zhang, Gengze Wu, Tao Zhuang, Xiaoyu Tian, Zhijie Liu, Junling Liu, Kun Sun, Fengyuan Chen, Yuzhen Zhang, Chunyu Zeng, Yu Huang, Bing Zhang
Summary: In this study, LARP7 is identified as an aging antagonist, and the ATM-LARP7-SIRT1-p53/p65 pathway plays a crucial role in DNA damage response-mediated cellular senescence and atherosclerosis.
Article
Biochemistry & Molecular Biology
Richa Virmani, Prashant Pradhan, Jayadev Joshi, Avril Luyang Wang, Hem Chandra Joshi, Andaleeb Sajid, Anoop Singh, Vishal Sharma, Bishwajit Kundu, Daniel Blankenberg, Virginie Molle, Yogendra Singh, Gunjan Arora
Summary: PrkC protein kinase plays a vital role in the phenotypic memory and germination of Bacillus anthracis spores, and the loss of PrkC-dependent phosphorylation affects spore development. The key metabolic enzyme Phosphoglycerate mutase (Pgm) is involved in spore germination by catalyzing the isomerization of 2-PGA and 3-PGA. This study demonstrates that PrkC phosphorylates and regulates Pgm, negatively regulating its activity and maintaining its apo-like isoform before germination.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Ignacio Rodriguez-Pastrana, Eleni Birli, Amanda S. Coutts
Summary: The tumor suppressor p53 plays a crucial role in the cellular response to DNA damage, including cell cycle arrest, apoptosis, and DNA repair. JMY, an actin nucleator and DNA damage-responsive protein, undergoes nuclear accumulation during DNA damage. Through transcriptomics, we identified that JMY is required for the regulation of key p53 target genes involved in DNA repair. Lack or knockout of JMY leads to increased DNA damage and JMY's actin nucleation function is necessary for promoting the clearance of DNA lesions. This study suggests a role for actin in JMY's nuclear activity during the DNA damage response.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Oncology
Lepakshe S. Madduri, Nichole D. Brandquist, Chitra Palanivel, Geoffrey A. Talmon, Michael J. Baine, Sumin Zhou, Charles A. Enke, Keith R. Johnson, Michel M. Ouellette, Ying Yan
Summary: This study reveals a novel mechanism by which p53 regulates the stability of PR55α protein via FBXL20, demonstrating that p53 deficiency leads to increased PR55α stability, thereby promoting the growth and proliferation of pancreatic cancer cells.
Article
Oncology
Sonja J. Gill, Paul W. G. Wijnhoven, Jacqueline H. L. Fok, Rebecca L. Lloyd, Jonathan Cairns, Joshua Armenia, Jenni Nikkila, Alan Lau, Christopher J. Bakkenist, Susan M. Galbraith, Conchita Vens, Mark J. O'Connor
Summary: Radiopotentiation profiling of DNA damage response inhibitors (DDRi) revealed lower effective concentrations in radiotherapy combinations, with preferential sensitization of p53-deficient cells. Olaparib, ceralasertib, and adavosertib showed moderate increases in radiotherapy dose enhancement with increasing inhibitor concentration, while small increases in AZD0156 and KU-60648 resulted in steep increases in dose enhancement. Multiple concentration testing demonstrated a relationship between drug concentration and radiotherapy effect, providing valuable insights for dose-escalation strategies in clinical trials.
MOLECULAR CANCER THERAPEUTICS
(2021)
Article
Microbiology
Chunguang Liang, Ana B. Rios-Miguel, Marcel Jarick, Priya Neurgaonkar, Myriam Girard, Patrice Francois, Jacques Schrenzel, Eslam S. Ibrahim, Knut Ohlsen, Thomas Dandekar
Summary: The serine/threonine kinase PknB and its corresponding phosphatase Stp play important roles in regulating various cell functions in the pathogen S. aureus. They impact amino acid catabolism, gluconeogenesis, and synthesis of key cellular components. The study also highlighted interactions of PknB with CdaA and the yvcK/glmR regulon, as well as the phosphorylation-mediated decrease in expression of virulence factors in S. aureus.
Article
Biochemistry & Molecular Biology
Karla Gisel Calderon-Gonzalez, Ixaura Medina-Medina, Lucia Haronikova, Lenka Hernychova, Ondrej Bonczek, Lukas Uhrik, Vaclav Hrabal, Borivoj Vojtesek, Robin Fahraeus, Jesus Hernandez-Monge, Vanesa Olivares-Illana
Summary: HDMX and HDM2 are essential proteins in the cell, with HDMX playing a role in regulating the activity of p53. Despite having high similarity in their RING domains, HDMX does not possess E3 ligase activity like HDM2. However, HDMX is still important for the proper poly-ubiquitination of p53. The phosphorylation of HDM2 at serine 395 changes its conformation and activity, but the effect of phosphorylation on HDMX is still unknown. In this study, the researchers investigate the conformation and E3 ligase activity of HDMX, as well as its binding with p53.
BIOSCIENCE REPORTS
(2022)
Article
Multidisciplinary Sciences
Jonathan Cowan, Michael R. Longman, Andrew K. Snabaitis
Summary: The study investigates the impact of reduced expression of the alpha 4 protein on cardiomyocytes, showing that alpha 4 knockdown increases DNA damage and cell death, indicating an anti-apoptotic role of alpha 4 in the heart. Additionally, alpha 4 knockdown reduces the expression of anti-apoptotic proteins, increases phosphorylation of p53 and ATM/ATR substrates, and enhances sensitivity to cytotoxic drugs.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Reika Iwakawa, Takashi Kohno, Masato Enari, Tohru Kiyono, Jun Yokota
Article
Oncology
Yuudai Kondo, Kentaro Nagai, Shingo Nakahata, Yusuke Saito, Tomonaga Ichikawa, Akira Suekane, Tomohiko Taki, Reika Iwakawa, Masato Enari, Masafumi Taniwaki, Jun Yokota, Sumio Sakoda, Kazuhiro Morishita
Article
Cell Biology
Yoshie Endo, Atsumi Sugiyama, Shun-Ai Li, Kazuji Ohmori, Hirokazu Ohata, Yusuke Yoshida, Masabumi Shibuya, Kohji Takei, Masato Enari, Yoichi Taya
Article
Biochemistry & Molecular Biology
Hirokazu Ohata, Nobuyuki Ota, Mikako Shirouzu, Shigeyuki Yokoyama, Jun Yokota, Yoichi Taya, Masato Enari
JOURNAL OF MOLECULAR BIOLOGY
(2009)
Article
Biochemistry & Molecular Biology
Hirokazu Ohata, Makoto Miyazaki, Ryo Otomo, Yuko Matsushima-Hibiya, Chihiro Otsubo, Takahiro Nagase, Hirofumi Arakawa, Jun Yokota, Hitoshi Nakagama, Yoichi Taya, Masato Enari
MOLECULAR AND CELLULAR BIOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Takashi Kohno, Hitoshi Ichikawa, Yasushi Totoki, Kazuki Yasuda, Masaki Hiramoto, Takao Nammo, Hiromi Sakamoto, Koji Tsuta, Koh Furuta, Yoko Shimada, Reika Iwakawa, Hideaki Ogiwara, Takahiro Oike, Masato Enari, Aaron J. Schetter, Hirokazu Okayama, Aage Haugen, Vidar Skaug, Suenori Chiku, Itaru Yamanaka, Yasuhito Arai, Shun-ichi Watanabe, Ikuo Sekine, Seishi Ogawa, Curtis C. Harris, Hitoshi Tsuda, Teruhiko Yoshida, Jun Yokota, Tatsuhiro Shibata
Article
Biochemistry & Molecular Biology
K. Ohmori, Y. Endo, Y. Yoshida, H. Ohata, Y. Taya, M. Enari
Article
Cell Biology
M Enari, K Ohmori, I Kitabayashi, Y Taya
GENES & DEVELOPMENT
(2006)
Article
Multidisciplinary Sciences
PC Klöhn, L Stoltze, E Flechsig, M Enari, C Weissmann
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2003)
Article
Multidisciplinary Sciences
C Weissmann, M Enari, PC Klöhn, D Rossi, E Flechsig
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2002)
Article
Immunology
C Weissmann, M Enari, PC Klöhn, D Rossi, E Flechsig
JOURNAL OF INFECTIOUS DISEASES
(2002)
Article
Biochemistry & Molecular Biology
E Flechsig, I Hegyi, M Enari, P Schwarz, J Collinge, C Weissmann
MOLECULAR MEDICINE
(2001)
Article
Multidisciplinary Sciences
M Enari, E Flechsig, C Weissmann
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2001)
Review
Biochemistry & Molecular Biology
M Enari