Article
Biology
C. Maresca, A. Dello Stritto, C. D'Angelo, E. Petti, A. Rizzo, E. Vertecchi, F. Berardinelli, L. Bonanni, A. Sgura, A. Antoccia, G. Graziani, A. Biroccio, E. Salvati
Summary: PARP1 interacts with TRF1 and modifies its DNA affinity, influencing telomere replication and helicase recruitment. This study uncovers a new role for PARP1 as a surveillant of telomere replication, orchestrating protein dynamics at the replication fork.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Masato Mashimo, Momoko Kita, Arina Uno, Moe Nii, Moe Ishihara, Takuya Honda, Yuka Gotoh-Kinoshita, Atsuo Nomura, Hiroyuki Nakamura, Toshihiko Murayama, Ryoichi Kizu, Takeshi Fujii
Summary: Poly(ADP-ribosyl)ation is a post-translational modification process that transfers poly(ADP-ribose) to proteins. TNKS1/2 have been found to play important roles in neuronal development, promoting neurite outgrowth and synapse formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Zhenzhen Li, Zhen Guo, Rui Lan, Sidong Cai, Zhirong Lin, Jingyan Li, Junjian Wang, Zhuoming Li, Peiqing Liu
Summary: BRD4 is crucial in the pathogenesis of cardiac hypertrophy by interacting with PARP1 to induce hypertrophic gene expression and transcription activation. Targeting the inhibition of PARP1-BRD4 interactions may have therapeutic potential for pathological cardiac hypertrophy.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Biology
Pierre-Olivier Esteve, Sagnik Sen, Udayakumar S. Vishnu, Cristian Ruse, Hang Gyeong Chin, Sriharsa Pradhan
Summary: SET8, the enzyme responsible for H4K20me1, is post-translationally poly ADP-ribosylated by PARP1, leading to its degradation and aberrant H4K20 methylation. This regulation plays important roles in mitotic condensation, DNA replication, DNA damage response, and gene expression.
COMMUNICATIONS BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Jeffrey Wang, Mohamed A. Ghonim, Salome Ibba, Hanh H. Luu, Yucel Aydin, Peter A. Greer, A. Hamid Boulares
Summary: This study reveals that poly(ADP-ribosyl)ation plays a critical role in protecting STAT6 from degradation, and can be synthetically targeted for degradation by inhibiting PARP-1. Additionally, the study identifies STAT6 as a bonafide substrate for chaperone-mediated autophagy in the human Jurkat cell-line.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Konstantin N. Naumenko, Mariya V. Sukhanova, Loic Hamon, Tatyana A. Kurgina, Rashid O. Anarbaev, Aswin Mangerich, David Pastre, Olga I. Lavrik
Summary: Y-box-binding protein 1 (YB-1) is involved in the regulation of gene expression and has been found to play a role in the regulation of PARP1 activity. The C-terminal domain of YB-1 is able to interact with PAR and control its synthesis, providing important insights into the regulation of PARP1 activity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Guillaume Bordet, Iaroslava Karpova, Alexei Tulin
Summary: The transcriptome undergoes rapid and massive changes during developmental stage transitions, necessitating tight control by chromatin factors to prevent undesired reactivation of gene expression. PARP-1 and PARG work together to regulate the gene expression profile during the larval/pupa transition, ensuring integrity of the expression profile between developmental stages.
SCIENTIFIC REPORTS
(2022)
Review
Oncology
Giulia Pinton, Sara Boumya, Maria Rosa Ciriolo, Fabio Ciccarone
Summary: This review article explores the role of PARP-1 and poly(ADP-ribosyl)ation in gene expression, DNA repair pathways, and genomic stability, including their impact on chromatin remodelling. The article specifically focuses on how PARP-1 directly modifies histone proteins and enzymes involved in DNA/histone epigenetic modifications to shape chromatin structure during transcription and DNA damage response. Understanding the role of poly(ADP-ribosyl)ation in regulating chromatin organization could provide insights into resistance mechanisms to PARP inhibitors and the clinical relevance of combining epigenetic drugs.
Article
Multidisciplinary Sciences
Jyothi Mahadevan, Asmita Jha, Johannes Rudolph, Samuel Bowerman, Domenic Narducci, Anders S. Hansen, Karolin Luger
Summary: PARP1 contributes to genome architecture and DNA damage repair through its dynamic association with chromatin. PARP1/2 recognize damaged DNA and recruit the DNA repair machinery. Single-molecule microscopy in live cells revealed two classes of freely diffusing PARP1/2 and two classes of bound PARP1/2. Majority of PARP1/2 diffuse freely in both undamaged and damaged nuclei, while a small fraction becomes transiently bound upon laser-induced DNA damage. Treatment with PARP1/2 inhibitors in the presence of DNA damage causes subtle changes in the dynamics of bound PARP1/2, suggesting potential for targeted therapy.
Review
Cell Biology
Palmiro Poltronieri, Angela Celetti, Luca Palazzo
Summary: NAD(+) is crucial in post-translational modification of proteins and nucleic acids, with its synthesis, degradation, and transport playing a vital role in maintaining optimal NAD(+) levels. The functional connection between NAD(+-utilizing enzymes and mono(ADP-ribosyl)ating enzymes is linked to chronic diseases.
Article
Biochemistry & Molecular Biology
Liang Kong, Baomin Feng, Yan Yan, Chao Zhang, Jun Hyeok Kim, Lahong Xu, Johannes Gregor Matthias Rack, Ying Wang, Jyan-Chyun Jang, Ivan Ahel, Libo Shan, Ping He
Summary: This study reveals that the noncanonical ADP-ribosyltransferase SRO2 mediates the MARylation of zinc finger proteins SZF1 and SZF2, key regulators of immune gene expression. MARylation antagonizes polyubiquitination of SZF1, stabilizing the protein and maintaining protein homeostasis to mediate immune responses.
Article
Biochemistry & Molecular Biology
T. A. Kurgina, O. I. Lavrik
Summary: Poly(ADP-ribose) (PAR) is a negatively charged polymer composed of ADP-ribose monomers that is synthesized by poly(ADP-ribose)polymerase (PARP) enzymes upon DNA damage. PARP1 and PARP2 are the most well-studied members of the PARP family and play crucial roles in regulating DNA repair. A newly discovered histone PARylation factor (HPF1) modulates the activity of PARP1/2 by forming a transient joint active site with them. The review discusses the discovery and classical mechanism of PARylation in higher eukaryotes, as well as the role of HPF1 in this process.
Article
Biochemistry & Molecular Biology
Chuanchao Zhang, Bo Zhou, Feng Gu, Hongmei Liu, Honglin Wu, Fuwen Yao, Hui Zheng, Hui Fu, Wei Chong, Shurui Cai, Min Huang, Xiaolu Ma, Zhifang Guo, Tingting Li, Wenyuan Deng, Meiwen Zheng, Qiao Ji, Yongliang Zhao, Yongjie Ma, Qi-En Wang, Tie-Shan Tang, Caixia Guo
Summary: This study identifies a human long noncoding RNA (lncRNA)-derived micropeptide, PACMP, which functions to maintain CtIP abundance and promote poly(ADP-ribosyl)ation. Targeting PACMP inhibits tumor growth through synthetic lethal interaction between CtIP and PARP inhibitions, and enhances sensitivity to various anticancer drugs and radiation.
Article
Biochemistry & Molecular Biology
Dongsheng Yao, Marcus A. Arguez, Ping He, Andrew F. Bent, Junqi Song
Summary: PARylation and K63-linked ubiquitination coordinately regulate plant immunity, with the PARylation of UBC13 regulating K63-linked ubiquitination of PDIs, which may further promote correct protein folding and secretion of PDIs.
Article
Multidisciplinary Sciences
Fabian Konrath, Michael Willenbrock, Dorothea Busse, Claus Scheidereit, Jana Wolf
Summary: The activation of IKK/NF-kappa B is a crucial process in the DNA damage response, affecting cell-fate decisions and interpathway regulation. This study reveals a pathway structure with two hubs and demonstrates how changes in individual components impact pathway activity. It provides mechanistic understanding relevant for experimental and clinical studies.
Article
Biochemistry & Molecular Biology
Martin Bachman, Santiago Uribe-Lewis, Xiaoping Yang, Heather E. Burgess, Mario Iurlaro, Wolf Reik, Adele Murrell, Shankar Balasubramanian
NATURE CHEMICAL BIOLOGY
(2015)
Article
Biotechnology & Applied Microbiology
Santiago Uribe-Lewis, Rory Stark, Thomas Carroll, Mark J. Dunning, Martin Bachman, Yoko Ito, Lovorka Stojic, Silvia Halim, Sarah L. Vowler, Andy G. Lynch, Benjamin Delatte, Eric J. de Bony, Laurence Colin, Matthieu Defrance, Felix Krueger, Ana-Luisa Silva, Rogier ten Hoopen, Ashraf E. K. Ibrahim, Francois Fuks, Adele Murrell
Article
Multidisciplinary Sciences
Lovorka Stojic, Malwina Niemczyk, Arturo Orjalo, Yoko Ito, Anna Elisabeth Maria Ruijter, Santiago Uribe-Lewis, Nimesh Joseph, Stephen Weston, Suraj Menon, Duncan T. Odom, John Rinn, Fanni Gergely, Adele Murrell
NATURE COMMUNICATIONS
(2016)
Article
Oncology
Helen Ross-Adams, Stephen Ball, Kate Lawrenson, Silvia Halim, Roslin Russell, Claire Wells, Siri H. Strand, Torben F. Orntoft, Melissa Larson, Sebastian Armasu, Charles E. Massie, Mohammad Asim, Martin M. Mortensen, Michael Borre, Kathryn Woodfine, Anne Y. Warren, Alastair D. Lamb, Jonathan Kay, Hayley Whitaker, Antonio Ramos-Montoya, Adele Murrell, Karina D. Sorensen, Brooke L. Fridley, Ellen L. Goode, Simon A. Gayther, John Masters, David E. Neal, Ian G. Mills
Article
Genetics & Heredity
Malwina Niemczyk, Yoko Ito, Joanna Huddleston, Anna Git, Sayeda Abu-Amero, Carlos Caldas, Gudrun E. Moore, Lovorka Stojic, Adele Murre
AMERICAN JOURNAL OF HUMAN GENETICS
(2013)
Article
Biochemistry & Molecular Biology
Liang Wu, Pierre Murat, Dijana Matak-Vinkovic, Adele Murrell, Shankar Balasubramanian
Article
Chemistry, Multidisciplinary
Martin Bachman, Santiago Uribe-Lewis, Xiaoping Yang, Michael Williams, Adele Murrell, Shankar Balasubramanian
Editorial Material
Genetics & Heredity
Adele Murrell
Article
Biochemistry & Molecular Biology
Yoko Ito, Raffaella Nativio, Adele Murrell
NUCLEIC ACIDS RESEARCH
(2013)
Article
Multidisciplinary Sciences
Benjamin Delatte, Jana Jeschke, Matthieu Defrance, Martin Bachman, Catherine Creppe, Emilie Calonne, Martin Bizet, Rachel Deplus, Laura Marroqui, Myriam Libin, Mirunalini Ravichandran, Francoise Mascart, Decio L. Eizirik, Adele Murrell, Tomasz P. Jurkowski, Francois Fuks
SCIENTIFIC REPORTS
(2015)
Article
Oncology
Claudia Fabiola Mendez-Catala, Svetlana Gretton, Alexander Vostrov, Elena Pugacheva, Dawn Farrar, Yoko Ito, France Docquier, Georgia-Xanthi Kita, Adele Murrell, Victor Lobanenkov, Elena Klenova
Review
Biochemistry & Molecular Biology
Giuseppina Pisignano, Ioanna Pavlaki, Adele Murrell
DNA PACKAGING: NUCLEOSOME AND CHROMATIN
(2019)
Article
Multidisciplinary Sciences
Santiago Uribe-Lewis, Thomas Carroll, Suraj Menon, Anna Nicholson, Piotr J. Manasterski, Douglas J. Winton, Simon J. A. Buczacki, Adele Murrell
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Basetti Madhu, Santiago Uribe-Lewis, Martin Bachman, Adele Murrell, John R. Griffiths
SCIENTIFIC REPORTS
(2020)
Article
Biotechnology & Applied Microbiology
Stephen Richer, Yuan Tian, Stefan Schoenfelder, Laurence Hurst, Adele Murrell, Giuseppina Pisignano
Summary: This study investigates the differences in three-dimensional chromatin conformation between heterozygous loci, taking into consideration parent-of-origin differences and genome-wide allele-specific chromatin conformation associations. A bioinformatic pipeline called HiCFlow is developed for haplotype assembly and visualization of parental chromatin architecture. The study identifies stable allele-specific interactions at specific gene loci and detects allele-specific differences in A/B compartmentalization. The findings highlight the widespread differences in chromatin conformation and provide insights into allele-specific expressed genes, including previously unidentified ones.