Article
Multidisciplinary Sciences
Helen Tanton, Tomasz Sewastianik, Hyuk-Soo Seo, David Remillard, Roodolph St Pierre, Pratyusha Bala, Daulet Aitymbayev, Peter Dennis, Keith Adler, Ezekiel Geffken, Zoe Yeoh, Nicholas Vangos, Filip Garbicz, David Scott, Nilay Sethi, James Bradner, Sirano Dhe-Paganon, Ruben D. Carrasco
Summary: The study identified a specific small-molecule inhibitor that can suppress Wnt/beta-catenin signaling and slow down the growth of colorectal cancer. This finding is of great significance for the development of novel therapies for colorectal cancer.
Article
Multidisciplinary Sciences
Shion Orikasa, Nobuyuki Kawashima, Kento Tazawa, Kentaro Hashimoto, Keisuke Sunada-Nara, Sonoko Noda, Mayuko Fujii, Tetsu Akiyama, Takashi Okiji
Summary: This study revealed that BCL9 plays a crucial role in the osteo/odontoblastic differentiation of dental pulp stem cells (DPSCs) under hypoxic conditions. This differentiation process is partially dependent on the Wnt/β-catenin signaling pathway, with BCL9 acting as a key mediator between HIF1 alpha and Wnt/β-catenin signaling.
SCIENTIFIC REPORTS
(2022)
Review
Chemistry, Medicinal
Hao Zhang, Ya Bao, Chenglong Liu, Jianqi Li, Di Zhu, Qingwei Zhang
Summary: The Wnt/β-catenin signaling pathway plays a crucial role in organism development and disease, making the direct targeting of protein-protein interactions a promising strategy for pathway inhibition. Despite the early stage of research in this area, there is hope for future development of inhibitors.
FUTURE MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Abhishek Sinha, Vinson B. Fan, Aravinda-Bharathi Ramakrishnan, Nicole Engelhardt, Jennifer Kennell, Ken M. Cadigan
Summary: SOX9 promotes turnover of β-catenin independently of the destruction complex and proteasome in mammalian cell culture. This requires the activation of MAML2, which acts as a coactivator for SOX9 in antagonizing the Wnt/β-catenin pathway. This newly identified pathway provides a parallel mechanism for restricting Wnt/β-catenin signaling.
Article
Immunology
Ruqing Gao, Xiaoqiang Zheng, Aimin Jiang, Wangxiao He, Tianya Liu
Summary: The tumor-specific biomimetic Wnt pathway suppressor CM-CA effectively inhibits the Wnt/β-catenin signaling pathway and cellular proliferation in lung adenocarcinoma, leading to significant tumor growth inhibition. CM-CA also promotes T cell infiltration and enhances the immune response within tumor tissues.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Yalei Lv, Yujie Shan, Lina Song, Yufei Zhao, Ruixue Lai, Junyan Su, Xiaoyun Zhang
Summary: This study found that high levels of ITGB1 expression in gastric cancer patients are associated with abundant expression of type I collagen and poor overall survival. Through in vitro experiments, it was demonstrated that ITGB1 may promote the activation of the BCL9L/beta-catenin signaling pathway, leading to chemo-resistance in gastric cancer cells. Blocking the beta-catenin signals improved the anticancer effects of chemotherapy, providing a potential new approach for clinical gastric cancer therapy.
Article
Chemistry, Medicinal
Zhen Wang, Min Zhang, Harriet M. Thompson, Haitao Ji
Summary: A series of new small-molecule beta-catenin/B-cell lymphoma 9 (BCL9) protein-protein interaction inhibitors were reported. Among them, compound 21 showed potent inhibition in cellular context and demonstrated higher potential in regulating transcription and expression of beta-catenin target genes and suppressing survival of beta-catenin-dependent cancer cells.
ACS MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Oncology
Shaojian Lin, Weiwei Zhang, Ziwen Shi, Langping Tan, Yue Zhu, Honghao Li, Xinzhi Peng
Summary: The study revealed the transcriptional regulation of LINC01278 by beta-catenin/LEF-1 transcription factor and its negative feedback regulation on beta-catenin signal in papillary thyroid carcinoma (PTC) cells. This negative regulation inhibits downstream target gene expression activated by the Wnt/beta-catenin pathway.
CANCER CELL INTERNATIONAL
(2021)
Article
Pharmacology & Pharmacy
Weiming You, Fang Ma, Zhang Zhang, Jin Yan
Summary: A gadolinium-gold-based core/shell nanostructure drug delivery system was developed in this study, which effectively enhanced tumor response to immune therapy and maintained good biosafety and biocompatibility.
Article
Developmental Biology
Gianluca Zambanini, Anna Nordin, Mattias Jonasson, Pierfrancesco Pagella, Claudio Cantu
Summary: We developed a modified version of CUT&RUN called LoV-U, which allows for stable and reproducible generation of beta-catenin binding profiles, uncovering its direct target genes in human cells and mouse tissues. This method outperforms the original CUT&RUN when targeting co-factors that do not bind DNA, and is suitable for processing multiple samples simultaneously.
Article
Biochemistry & Molecular Biology
Qiyang Xiao, Lijing Wang, Juling Zhang, Xinyu Zhong, Zhou Guo, Jiahao Yu, Yuanyuan Ma, Haigang Wu
Summary: This study investigates the effects of 660 nm laser radiation on mouse breast tissue, revealing that high dosage light exposure can cause differential gene expression and activate transcription-related pathways. The Wnt/β-catenin pathway appears to play a critical role in the response to 660 nm light exposure. Additionally, the 660 nm laser can affect nucleotide metabolism by regulating purine metabolism.
Article
Environmental Sciences
Bugao Guan, Jun Ma, Zhi Yang, Fei Yu, Jian Yao
Summary: The lncRNA NCK1-AS1 is highly expressed in gastric cancer and acts as an oncogene by competitively binding to miR-22-3p to upregulate BCL9 expression, leading to the activation of the Wnt/β-catenin signaling pathway and promoting gastric cancer progression.
ENVIRONMENTAL TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Min Gyu Lee, Hwamok Oh, Jong Woo Park, Jueng Soo You, Jeung-Whan Han
Summary: Nuclear S6K1 functions in the regulation of Wnt target gene expression and has an impact on cell proliferation and invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Mathias Wendt, Rosa Bellavita, Alan Gerber, Nina-Louisa Efrem, Thirza van Ramshorst, Nicholas M. Pearce, Paul R. J. Davey, Isabel Everard, Mercedes Vazquez-Chantada, Elisabetta Chiarparin, Paolo Grieco, Sven Hennig, Tom N. Grossmann
Summary: This study designed beta-sheet mimetics targeting intracellular protein beta-catenin, effectively inhibiting the Wnt signaling pathway. The presented design strategy can support the development of inhibitors for other beta-sheet-mediated protein-protein interactions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Multidisciplinary Sciences
Natoya J. Peart, Jae Yeon Hwang, Mathieu Quesnel-Vallieres, Matthew J. Sears, Yuequin Yang, Peter Stoilov, Yoseph Barash, Juw Won Park, Kristen W. Lynch, Russ P. Carstens
Summary: Epithelial splicing regulatory proteins ESRP1 and ESRP2 play crucial roles in the regulation of alternative splicing during mammalian development, particularly in genes involved in maintaining epithelial cell function. Through eCLIP and RNA-Seq analyses, it was found that ESRP1 primarily functions in splicing regulation by directly binding to specific sites to promote exon inclusion or skipping. Additionally, ESRP1 also exhibits widespread binding in the untranslated regions of genes related to epithelial cell function, suggesting its involvement in post-transcriptional regulation beyond splicing.
Article
Multidisciplinary Sciences
Virginia Andreani, Senthilkumar Ramamoorthy, Abhinav Pandey, Ekaterina Lupar, Stephen L. Nutt, Tim Laemmermann, Rudolf Grosschedl
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Immunology
Marta Derecka, Josip Stefan Herman, Pierre Cauchy, Senthilkumar Ramamoorthy, Ekaterina Lupar, Dominic Gruen, Rudolf Grosschedl
Article
Cell Biology
Senthilkumar Ramamoorthy, Kohei Kometani, Josip S. Herman, Marc Bayer, Soeren Boller, Joy Edwards-Hicks, Haribaskar Ramachandran, Rui Li, Ramon Klein-Geltink, Erika L. Pearce, Dominic Gruen, Rudolf Grosschedl
GENES & DEVELOPMENT
(2020)
Article
Immunology
Yuanting Wang, Nikolay Zolotarev, Cheng-Yuan Yang, Angelika Rambold, Gerhard Mittler, Rudolf Grosschedl
Retraction
Biochemistry & Molecular Biology
Oliver Bischof, Klaus Schwamborn, Nadine Martin, Andreas Werner, Claudio Sustmann, Rudolf Grosschedl, Anne Dejean
Article
Multidisciplinary Sciences
Tanya Kapoor, Mauro Corrado, Erika L. Pearce, Edward J. Pearce, Rudolf Grosschedl
SCIENTIFIC REPORTS
(2020)
Article
Cell Biology
Gustavo Antonio Urrutia, Haribaskar Ramachandran, Pierre Cauchy, Kyungjin Boo, Senthilkumar Ramamoorthy, Soeren Boller, Esen Dogan, Thomas Clapes, Eirini Trompouki, Maria -Elena Torres-Padilla, Jorma J. Palvimo, Andrea Pichler, Rudolf Grosschedl
Summary: The study shows that SUMOylation of SATB2 in ESCs may play a key role in regulating pluripotency and differentiation by impacting gene silencing and activation, as well as higher-order chromatin interactions.
GENES & DEVELOPMENT
(2021)
Article
Immunology
Roshni Roy, Senthilkumar Ramamoorthy, Benjamin D. Shapiro, Mary Kaileh, Dena Hernandez, Dimitra Sarantopoulou, Sampath Arepalli, Soeren Boller, Amit Singh, Arsun Bektas, Jaekwan Kim, Ann Zenobia Moore, Toshiko Tanaka, Julia McKelvey, Linda Zukley, Cuong Nguyen, Tonya Wallace, Christopher Dunn, Robert Wersto, William Wood, Yulan Piao, Kevin G. Becker, Christopher Coletta, Supriyo De, Jyoti Misra Sen, Alexis Battle, Nan-ping Weng, Rudolf Grosschedl, Luigi Ferrucci, Ranjan Sen
Summary: This study identified lineage-specific DNA methylation signatures of six immune cell types from human peripheral blood, with PU.1 transcription factor found to be associated with lineage-specific DNA methylation. The observations suggest that innate and adaptive immune lineages are specified by distinct epigenetic mechanisms.
Editorial Material
Biochemistry & Molecular Biology
Marc Bayer, Rudolf Grosschedl
Summary: Gain-of-function NOTCH1 mutations promote oncogenic MYC expression in T-ALL cells. Zhou et al. (2022) reveal that therapy-resistant T-ALL cells activate EBF1, leading to a T-to-B lineage shift and sustained MYC expression in the absence of Notch signaling.
Correction
Immunology
Roshni Roy, Senthilkumar Ramamoorthy, Benjamin D. Shapiro, Mary Kaileh, Dena Hernandez, Dimitra Sarantopoulou, Sampath Arepalli, So Ren Boller, Amit Singh, Arsun Bektas, Jaekwan Kim, Ann Zenobia Moore, Toshiko Tanaka, Julia McKelvey, Linda Zukley, Cuong Nguyen, Tonya Wallace, Christopher Dunn, Robert Wersto, William Wood, Yulan Piao, Kevin G. Becker, Christopher Coletta, Supriyo De, Jyoti Misra Sen, Alexis Battle, Nan-ping Weng, Rudolf Grosschedl, Luigi Ferrucci, Ranjan Sen
Article
Multidisciplinary Sciences
Zhaoping Qiu, Weijie Guo, Bo Dong, Yu Wang, Pan Deng, Chi Wang, Jinpeng Liu, Qing Zhang, Rudolf Grosschedl, Zhiyong Yu, Jiong Deng, Yadi Wu
Summary: EBF1 is highly expressed in TNBC and plays a crucial role in tumorigenicity and progression; By forming a transcriptional complex with HIF1 alpha, EBF1 regulates cell mitophagy and prevents cell death; Targeting EBF1 pathway may offer alternative treatment strategies for TNBC.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Marc Bayer, Soren Boller, Senthilkumar Ramamoothy, Nikolay Zolotarev, Pierre Cauchy, Norimasa Iwanami, Gerhard Mittler, Thomas Boehm, Rudolf Grosschedl
Summary: The study identified the nuclear import receptor Transportin-3 (Tnpo3) as an important regulator of the transcription factor EBF1, which plays a key role in B cell development. Tnpo3 ensures proper programming of B cells in nonpermissive conditions.
GENES & DEVELOPMENT
(2022)
Article
Immunology
Aurelie Lenaerts, Iwo Kucinski, Ward Deboutte, Marta Derecka, Pierre Cauchy, Thomas Manke, Berthold Gottgens, Rudolf Grosschedl
Summary: This study implicates EBF1 in regulating myeloid/lymphoid fate bias in MPPs by constraining C/EBP alpha-driven myelopoiesis and priming the B-lymphoid fate specifically in MPP3 cells.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Nikolay Zolotarev, Marc Bayer, Rudolf Grosschedl
Summary: The continuous activity of EBF1 is required for the stable maintenance of the transcriptional and epigenetic state of pro-B cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Iwo Kucinski, Nicola K. Wilson, Rebecca Hannah, Sarah J. Kinston, Pierre Cauchy, Aurelie Lenaerts, Rudolf Grosschedl, Berthold Goettgens