Anti-inflammatory effects of kolaviron modulate the expressions of inflammatory marker genes, inhibit transcription factors ERK1/2, p-JNK, NF-κB, and activate Akt expressions in the 93RS2 Sertoli cell lines

The anti-inflammatory effects of kolaviron (Kol-v) have been demonstrated in several experimental models. The ability of Kol-v to modulate the expressions of inflammatory genes in lipopolysaccharide (LPS)-stimulated Sertoli cell line, 93RS2 was investigated in this study. Kol-v decreased the expressions of inflammatory genes TNF-alpha, Tlr-4, and Nf kappa b1 and has synergistic effect on LPS-induced COX-2 and iNOS expressions at high concentrations (25-100 mu M). At lower concentrations (5-15 mu M), the expressions of TNF-alpha, IL-6, and IL-1 alpha were down-regulated by Kol-v except Tgf beta 1 that was up-regulated. The LPS-induced decrease in the expression of the anti-inflammatory genes IL-3, IL-4, and IL-10 was blocked by Kol-v at all concentrations of Kol-v tested. The LPS-induced phosphorylations of mitogen-activated protein kinase family members (ERK1/2, and p-JNK), decreased I kappa B alpha expression, and decreased Akt phosphorylation was blocked by Kol-v. Our results highlight the potential for Kol-v at lower concentration to ameliorate cellular damage caused by local inflammation.