4.6 Article

L-FABP T94A decreased fatty acid uptake and altered hepatic triglyceride and cholesterol accumulation in Chang liver cells stably transfected with L-FABP

期刊

MOLECULAR AND CELLULAR BIOCHEMISTRY
卷 345, 期 1-2, 页码 207-214

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SPRINGER
DOI: 10.1007/s11010-010-0574-7

关键词

Liver fatty acid-binding protein; Genetic polymorphism; Fatty acids; Triglyceride; Cholesterol; Chang liver cells

资金

  1. National Undergraduate Student Innovation Experimental Design Project [YA07048]
  2. Hunan Province Science and Technology Plan [2007FG3035]

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Liver fatty acid-binding protein (L-FABP, FABP1) is a highly conserved key factor in lipid metabolism. This study was undertaken to verify whether the T94A mutation in the L-FABP gene affects fatty acid uptake and intracellular esterification into specific lipid pools. Candidate SNPs were recreated using site-directed mutagenesis and tested for physical function in stably transfected Chang liver cell lines. We found that the T94A mutant of L-FABP lowered FFA uptake but had no effect on FFA efflux. L-FABP T94A-expressing cells showed decreased triglyceride content and increased cholesterol accumulation compared to the wild-type control for cells incubated with an FFA mixture (oleate: palmitate, 2: 1 ratio). In conclusion, our study provided additional indications of the functional relevance of the L-FABP T94A SNP in hepatic fatty acid and lipid metabolism in humans.

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