Article
Microbiology
Yaretzi J. Pedroza-Gomez, Raquel Cossio-Bayugar, Hugo Aguilar-Diaz, Silvana Scarcella, Enrique Reynaud, Maria del Rayo Sanchez-Carbente, Veronica Narvaez-Padilla, Estefan Miranda-Miranda
Summary: Bioinformatics analysis identified a putative carboxylesterase B enzyme in Fasciola hepatica, with its gene found in the complete genomes of two reported strains. Experimental assays confirmed the enzyme's molecular weight and activity. Further analysis suggested conservation of the enzyme's DNA sequences in other parasitic trematodes.
Article
Microbiology
Martha V. Fernandez-Baca, Cristian Hoban, Rodrigo A. Ore, Pedro Ortiz, Young-Jun Choi, Cesar Murga-Moreno, Makedonka Mitreva, Miguel M. Cabada
Summary: This study characterized a large sample of Fasciola hepatica with varying triclabendazole susceptibility to determine the complexity of resistance in this parasite. The results showed that resistance varied by geographic location, season, and morphology, providing a basis for further research on the association between resistance phenotype and genotype.
Article
Parasitology
Tania Rojas-Campos, Froylan Ibarra-Velarde, Yolanda Vera-Montenegro, Miguel Flores-Ramos, Irene Cruz-Mendoza, Gerardo Leyva-Gomez, Alicia Hernandez-Campos
Summary: This study compared the fasciolicidal efficacy of a new injectable formulation of fosfatriclaben with subcutaneous closantel and oral triclabendazole. Results showed that fosfatriclaben and triclabendazole had similar efficacy, but fosfatriclaben's intramuscular administration was more convenient and had lower dosage.
VETERINARY PARASITOLOGY
(2021)
Article
Parasitology
Carolin M. Morawietz, Alejandra M. Peter Ventura, Christoph G. Grevelding, Simone Haeberlein, Bernhard Spengler
Summary: Understanding drug penetration, distribution, and metabolization in parasites is essential for drug efficacy. Recently, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) has been used to visualize the distribution patterns of drug compounds in blood flukes and liver flukes. This technique provides high spatial resolution and has the potential to contribute to preclinical drug discovery in liver fluke research.
PARASITOLOGY RESEARCH
(2022)
Article
Parasitology
Froylan Ibarra-Velarde, Tania Rojas-Campos, Yolanda Vera-Montenegro, Miguel Flores-Ramos, Rafael Castillo, Gerardo Leyva-Gomez
Summary: The study aimed to determine the effective dose of fosfatriclaben through a field study in sheep naturally infected with F. hepatica. Results showed that fosfatriclaben had a high fasciolicidal efficacy similar to triclabendazole, with only half the dose required to remove eggs and flukes in the sheep studied.
PARASITOLOGY RESEARCH
(2022)
Article
Veterinary Sciences
Jane M. Kelley, Grant Rawlin, Travis Beddoe, Mark Stevenson, Terry W. Spithill
Summary: This study in Australia highlighted deficiencies in controlling Fasciola hepatica infection in dairy cattle, such as excessive use of clorsulon, limited rotation of flukicides, lack of diagnostic testing to inform treatment, and potential underdosing of animals. Communication gaps were identified in diagnostic testing for flukicide use, rotation of flukicide actives, flukicide administration, and testing of replacement animals.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Parasitology
Anand Chakroborty, Deiniol Pritchard, Marc E. Bouillon, Anna Cervi, Alan Cookson, Charlotte Wild, Caroline Fenn, Joseph Payne, Peter Holdsworth, Colin Capner, Jenna O'Neill, Gilda Padalino, Josephine Forde-Thomas, Sandeep Gupta, Brendan G. Smith, Maggie Fisher, Martina Lahmann, Mark S. Baird, Karl F. Hoffmann
Summary: Control of liver fluke infections is a significant challenge in the livestock sector due to drug resistant parasites. This study evaluated the anthelmintic activities of chemically modified abietic acid analogues against F. hepatica and found moderate activity in one analogue, but it also showed toxicity to mammalian cells. Therefore, further medicinal chemistry improvements are needed to consider these analogues as potential anthelmintics against liver fluke pathogens.
VETERINARY PARASITOLOGY
(2022)
Article
Parasitology
Monique Costa, Anderson Saravia, Diego Ubios, Pablo Lores, Valeria da Costa, Maria Florencia Festari, Mercedes Landeira, Santiago A. Rodriguez-Zraquia, Georgget Banchero, Teresa Freire
Summary: The aim of this research was to investigate the relationship between hepatic function, hematological parameters, leukocyte counts in circulation, parasite egg shedding, and the effects of triclabendazole (TCZ) treatment during Fasciola hepatica infection in cattle. The results showed increased levels of serum aspartate aminotransferase (AST) and gamma glutamyltransferase (GGT) in the early stages of infection. Additionally, high circulating eosinophil count and plateletcrit levels were correlated with the number of flukes in the livers of infected cattle. TCZ treatment reduced parasite burden and liver damage in chronically infected cattle but did not completely eliminate them.
EXPERIMENTAL PARASITOLOGY
(2022)
Article
Genetics & Heredity
Estefan Miranda-Miranda, Silvana Scarcella, Enrique Reynaud, Veronica Narvaez-Padilla, Gisela Neira, Roberto Mera-Y-Sierra, Hugo Aguilar-Diaz, Raquel Cossio-Bayugar
Summary: The anthelmintic resistance of Fasciola hepatica may be related to the catalytic activity of xenobiotic metabolizing enzymes. An inducible enzyme, carboxylesterase B (CestB), was found to have a single nucleotide polymorphism at position 643 resulting in an amino acid substitution at position 215. This mutation affects the enzyme's affinity to inhibitors and ester ligands. A field genotyping survey showed the distribution of different alleles in parasites from Argentina and Mexico, without any linkage to host species or geographic origin.
Article
Pharmacology & Pharmacy
Anand Chakroborty, Deiniol R. R. Pritchard, Marc E. E. Bouillon, Anna Cervi, Rolf Kraehenbuehl, Charlotte Wild, Caroline Fenn, Peter Holdsworth, Colin Capner, Gilda Padalino, Josephine E. E. Forde-Thomas, Joseph Payne, Brendan G. G. Smith, Maggie Fisher, Martina Lahmann, Mark S. S. Baird, Karl F. F. Hoffmann
Summary: This study explores the ex vivo anthelmintic potential of synthetic derivatives of hederagenin isolated from Hedera helix. The most active compound identified was MC042, which showed good selectivity against immature and adult flukes.
Article
Veterinary Sciences
Rafael Zafra, Leandro Buffoni, Raul Perez-Caballero, Veronica Molina-Hernandez, Maria T. Ruiz-Campillo, Jose Perez, Alvaro Martinez-Moreno, Francisco J. Martinez Moreno
Summary: In this study, sheep were vaccinated with two different vaccines containing a cocktail of antigens and formulated with different adjuvants. The results showed that the first vaccine significantly reduced fluke burden, decreased egg output, and alleviated hepatic lesions. Additionally, animals in the first vaccine group exhibited higher levels of IgG1 and IgG2 in the serological study, supporting the hypothesis of protection found in this group.
VETERINARY RESEARCH
(2021)
Article
Parasitology
Zeynep Ergenc, Eda Kepenekli, Nurhayat Yakut, Ozge Yapici, Utku Batu, Engin Tutar
Summary: This case report presents the successful treatment of pediatric fascioliasis using metronidazole. The patient was cured and recovered using a course of metronidazole.
IRANIAN JOURNAL OF PARASITOLOGY
(2022)
Article
Biochemical Research Methods
Clare F. Collett, Helen C. Phillips, Maggie Fisher, Sian Smith, Caroline Fenn, Phil Goodwin, Russell M. Morphew, Peter M. Brophy
Summary: Fasciola hepatica, the common liver fluke, has significant impacts on both animal health and food security, causing substantial economic losses globally. It is also re-emerging as a foodborne human disease. This study focused on cathepsin proteases, which are important targets for vaccines and diagnostics. The study revealed that cathepsin L zymogens contain highly antigenic epitopes and showed promising efficacy as markers for infection and treatment monitoring.
JOURNAL OF PROTEOME RESEARCH
(2022)
Review
Agriculture, Dairy & Animal Science
Gemma Zerna, Terry W. Spithill, Travis Beddoe
Summary: Maintaining sustainable agricultural systems is crucial for feeding the growing population. Parasite infections impact livestock production, including goats. Current liver fluke control methods involve drug application and pasture management.
Article
Infectious Diseases
Angelica Terashima, Marco Canales, Vicente Maco, A. Luis Marcos
Summary: This retrospective study in Peru investigated alternative TCBZ regimens for patients with chronic fascioliasis who failed the standard-of-care treatment, showing a cure rate of 74%. The term 'TCBZ resistance' may need to be re-evaluated using biomarkers to guide treatment decisions.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Infectious Diseases
O. Escobar-Chavarria, R. Cossio-Bayugar, P. Ramirez-Noguera, M. G. Prado-Ochoa, A. M. Velazquez-Sanchez, M. A. Munoz-Guzman, E. Angeles, F. Alba-Hurtado
Summary: Ethyl-4-bromophenylcarbamate (LQM 919) and ethyl-4-chlorophenylcarbamate (LQM 996) have the ability to induce apoptosis in Rhipicephalus microplus ovarian cells, as well as tick and mammalian cell cultures, displaying a significant acaricidal effect.
TICKS AND TICK-BORNE DISEASES
(2021)
Article
Biology
Sana Naseer, Javed Iqbal, Abeel Naseer, Sobia Kanwal, Ishtiaq Hussain, Yong Tan, Liliana Aguilar-Marcelino, Raquel Cossio-Bayugar, Zbigniew Zajac, Yousef A. Bin Jardan, Tariq Mahmood
Summary: Despite advancements in modern medicine, plant-derived medicines continue to be widely used due to their minimal side effects, low cost, and biocompatibility. This study evaluated the biological potential of methanolic and chloroform extracts from the roots, leaves, seeds, and flowers of Saussurea lappa. The extracts exhibited antioxidant, antibacterial, and cytotoxic activities, indicating their potential therapeutic applications. Further studies are recommended to isolate and characterize the bioactive compounds present in these plant parts for drug development.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Review
Biology
Adil Khan, Arif Ahmed Muhammed, Nasreen Nasreen, Furhan Iqbal, Raquel Cossio-Bayugar, Said Sajjad Ali Sha, Abdullah D. Alanazi, Zbigniew Zajac
Summary: Livestock industry plays a vital role in Pakistan's economy, however, small ruminants are at risk of haemoparasitic diseases transmitted by ticks. There is limited knowledge about tick-borne diseases and their vectors in goats and sheep in Pakistan, highlighting the need for further research and control measures.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Farhat Gul, Ilham Khan, Javed Iqbal, Banzeer Ahsan Abbasi, Amir Shahbaz, Raffaele Capasso, Itzel Amaro-Estrada, Yousef A. Bin Jardan, Raquel Cossio-Bayugar, Tariq Mahmood
Summary: The current study evaluated the phytochemicals and their potentials in Oxalis pes-caprae. The plant exhibited rich phenolic, flavonoid, alkaloid, and saponin contents, as well as significant antioxidative, antibacterial, and cytotoxic activities. Molecular docking analysis revealed that certain components of Oxalis pes-caprae showed good affinity with the SARS CoV-2 protein.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Genetics & Heredity
Estefan Miranda-Miranda, Silvana Scarcella, Enrique Reynaud, Veronica Narvaez-Padilla, Gisela Neira, Roberto Mera-Y-Sierra, Hugo Aguilar-Diaz, Raquel Cossio-Bayugar
Summary: The anthelmintic resistance of Fasciola hepatica may be related to the catalytic activity of xenobiotic metabolizing enzymes. An inducible enzyme, carboxylesterase B (CestB), was found to have a single nucleotide polymorphism at position 643 resulting in an amino acid substitution at position 215. This mutation affects the enzyme's affinity to inhibitors and ester ligands. A field genotyping survey showed the distribution of different alleles in parasites from Argentina and Mexico, without any linkage to host species or geographic origin.
Article
Genetics & Heredity
Saman Saman, Chien-Chin Chen, Nosheen Malak, Afshan Khan, Nasreen Nasreen, Adil Khan, Sadaf Niaz, Gauhar Rehman, Roger I. I. Rodriguez-Vivas, Raquel Cossio-Bayugar
Summary: The acaricidal activity of Datura innoxia ethanolic plant extract against Rhipicephalus microplus was studied using the adult immersion test and larval packet test. In vitro experiments and molecular docking simulations showed that the plant extract had consistent acaricidal activity and strong interactions with the target protein. This study also established the inhibitory effect of the extract on oviposition and larval mortality, indicating its potential use in tick management.
Article
Agriculture, Dairy & Animal Science
Sana Ayub, Nosheen Malak, Raquel Cossio-Bayugar, Nasreen Nasreen, Afshan Khan, Sadaf Niaz, Adil Khan, Abdallah D. Alanazi, Mourad Ben Said
Summary: A recent study explored the effectiveness of Pinus roxburghii plant extract in controlling populations of Rhipicephalus (Boophilus) microplus ticks. The study found that the plant extract showed high efficacy in vitro against different tick stages and demonstrated strong binding interaction between the plant's phytochemicals and the tick protein. This suggests the potential use of P. roxburghii as a promising anti-tick agent.
Article
Multidisciplinary Sciences
Cesar A. Arreguin-Perez, Estefan Miranda -Miranda, Jorge Folch-Mallol, Eduardo Ferrara-Tijera, Raquel Cossio-Bayugar
Summary: The southern cattle tick Rhipicephalus microplus is the most important bovine ectoparasite, transmitting lethal cattle diseases and causing billions of dollars in losses for the global livestock industry. Pesticide resistance has developed in cattle ticks due to decades of chemical treatment, leading to a need for research on biocontrol alternatives. Acaro-pathogenic microorganisms, such as Aspergillus flavus strain INIFAP-2021, show potential as biocontrol agents against R. microplus.
Article
Microbiology
Hugo Aguilar-Diaz, Rosa Estela Quiroz-Castaneda, Ixchel Guadalupe Diaz-Esquivel, Raquel Cossio-Bayugar, Estefan Miranda-Miranda
Summary: The search for alternatives to control ticks and tick-borne diseases is an ongoing issue. This study identified an estrogen-related receptor gene in cattle ticks and investigated its interaction with different estrogens, suggesting its potential as a target for tick control strategies.
Article
Microbiology
Cesar A. Arreguin-Perez, Estefan Miranda-Miranda, Jorge Luis Folch-Mallol, Raquel Cossio-Bayugar
Summary: In this study, an A. flavus strain isolated from naturally infected cattle ticks was found to possess a range of gene-coded virulence factors. Experimental bioassays showed that this strain exhibited high efficacy in controlling ticks, causing high morbidity and mortality rates, as well as inhibiting oviposition and reproduction.
Article
Microbiology
Farhan Ahmad Atif, Sami Ullah, Raquel Cossio-Bayugar, Muhammad Kashif, Aman Ullah Khan, Wen-Feng Wu
Summary: This study investigated the molecular prevalence, risk factors, and phylogenetic analysis of A. ovis infection in sheep and goats in different agro-climatic regions of Central and Southern Punjab, Pakistan. The overall molecular prevalence of anaplasmosis was 57.5%, with a higher occurrence in goats than in sheep. The genetic diversity of A. ovis strains varied between goat and sheep isolates.
Article
Microbiology
Estefan Miranda-Miranda, Raquel Cossio-Bayugar, Lauro Trejo-Castro, Hugo Aguilar-Diaz
Summary: The expression of the CestB gene in Fasciola hepatica is induced by exposure to the anthelmintic triclabendazole (TCBZ), resulting in increased enzyme activity. Variations in the gene sequence can lead to radical amino acid substitutions at the ligand binding site, potentially impacting the interaction and catalytic properties of the enzyme. This study identified novel CestB polymorphisms in TCBZ-resistant parasites and evaluated their effects on the binding of the metabolically oxidized form of TCBZ (TCBZSOX) using 3D modeling. The findings suggest that these polymorphisms create a binding pocket for TCBZSOX and may contribute to drug sequestration type of anthelmintic resistance. These results lay the groundwork for future development of a molecular diagnostic tool for identifying anthelmintic resistance in F. hepatica.
Article
Biology
Mohammed Ageeli Hakami, Nosheen Malak, Afshan Khan, Hidayat Ullah, Raquel Cossio-Bayugar, Nasreen Nasreen, Sadaf Niaz, Adil Khan, Chien-Chin Chen
Summary: This study explored the acaricidal effect and inhibitory mechanisms of Camellia sinensis plant extracts on ticks and mites. The results showed that the ethanolic plant extracts of C. sinensis exhibited high efficacy against mites and different stages of ticks in in vitro tests. In silico molecular docking revealed strong binding interactions between ellagic acid and the SsGST protein, as well as between quercetin and the RmGST protein.
Article
Multidisciplinary Sciences
Estefan Miranda-Miranda, Raquel Cossio-Bayugar, Hugo Aguilar-Diaz, Veronica Narvaez-Padilla, Bernardo Sachman-Ruiz, Enrique Reynaud
Summary: Fasciola hepatica is a globally distributed zoonotic parasitic worm that has developed resistance to anthelmintic compounds, prompting scientific efforts to study the molecular basis of this resistance for improved treatments. Transcriptome sequencing of sensitive and resistant strains treated with Triclabendazole and Albendazole revealed numerous new genes and RNA transcripts, providing insights into the mechanisms of resistance in these parasites.
