期刊
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
卷 185, 期 2, 页码 127-136出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2012.08.001
关键词
Translation; Leishmania; DED1; VAS; DEAD-box protein; Trypanosoma
资金
- Israel Science Foundation (ISF) [395/09]
- US-Israel Binational Foundation (BSF) [2007287]
- Division of Computing and Communication Foundations
- Direct For Computer & Info Scie & Enginr [2007287] Funding Source: National Science Foundation
DED1/VAS belong to the DEAD-box family of RNA helicases that are associated with translation initiation in higher eukaryotes. Here we report on two DED1/VAS homologs that were identified in the genome of Leishmania. The two paralogs include all the domains that are typical of DEAD-box proteins and a phylogenetic analysis suggests that their duplication predates the branching of DED1 and VAS, which took place along with the appearance of early metazoans. The two Leishmania DED1 paralogs complement a yeast strain that fails to express the endogenous DED1, suggesting that they are responsible for a similar function. This is also supported by RNAi-mediated silencing experiments performed in Trypanosoma brucei. The two proteins are functionally redundant, since defects in protein synthesis and cell growth arrest were observed only when both paralogs were eliminated. A partial stage-specific specialization is observed, as LeishDED1-2 is more abundant in promastigotes, whereas expression of LeishDED1-1 increases in amastigotes. Duplication of an essential gene usually offers a safety net against mutations but in this case it also generated two proteins with stage specific expression. (C) 2012 Elsevier B.V. All rights reserved.
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