期刊
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
卷 169, 期 2, 页码 108-114出版社
ELSEVIER
DOI: 10.1016/j.molbiopara.2009.10.009
关键词
Parasite; FACS; Macrophages; Bone marrow; Secreted proteins
资金
- CSBE Centre
- BBSRC
- EPSRC
- BBSRC [BB/D019621/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/D019621/1] Funding Source: researchfish
Investigating the proteome of intracellular Leishmania amastigotes has recently become possible due to the exploitation of fluorescence activated intracellular parasite sorting. Here, we employed this technology in combination with gel free analysis to greatly improve proteome coverage and suggest proteins putatively secreted by the parasites. In total, 1764 proteins were identified of which 741 had not been reported before. Protein abundance indices were calculated to rank individual proteins according to their abundance in vivo. Using the LeishCyc resource, an overview of metabolically relevant proteins was produced that integrated protein abundance data. Bioinformatic analysis identified 143 proteins possibly secreted by L. mexicana amastigotes, half of which have no known function. The data provide a useful resource, e.g. for modelling metabolic flux or selecting novel vaccine antigens. (C) 2009 Elsevier B.V. All rights reserved.
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