期刊
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
卷 165, 期 2, 页码 142-152出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2009.01.014
关键词
Leishmania; Life cycle; Maxicircle; Divergent region; Heteroplasmy
资金
- Russian Foundation for Basic Research [05-04-48895a]
- International Association for the promotion of co-operation with scientists from the New Independent States of the former Soviet Union (INTAS) [2001-216, 05-1000008-8043]
The kinetoplast genome contains several thousands of minicircles of various sequence classes and several scores of maxicircles. We demonstrated that maxicircles are heterogeneous in clonal cultures of Leishmania major, and, therefore, probably heterogeneous (heteroplasmic) within the kinetoplast. Sequence heterogeneity was observed in a non-coding fragment upstream of the 12S rRNA gene. We identified about 20 stable variants of this fragment, which were composed of one to five non-identical repeats 200-300 bp in length. Promastigote-to-amastigote and amastigote-to-promastigote differentiation was often accompanied by shifts in abundance of some maxicircle classes. Reversion to promastigote-specific maxicircle patterns was usually observed in the life cycle (promastigote-amastigote-promastigote), however there were many exceptions. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据