Article
Plant Sciences
Lu Zhai, Lingli Xie, JinSong Xu, Benbo Xu, Jing Dong, XueKun Zhang
Summary: This study investigated the effects and mechanisms of Thidiazuron (TDZ) treatment on seed size of Brassica napus, providing important insights for improving rapeseed yield.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Ti Zhang, Sally Vail, Hema S. N. Duddu, Isobel A. P. Parkin, Xulin Guo, Eric N. Johnson, Steven J. Shirtliffe
Summary: Phenotyping crop performance is crucial for plant breeding. Utilizing vegetation indices and aerial imagery from UAVs can effectively estimate canola flower numbers and develop models for predicting canola seed yield. The normalized difference yellowness index (NDYI) and flowering pixel numbers extracted from imagery over time show promise in predicting canola yield.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
Esmaeil Zangani, Kamran Afsahi, Farid Shekari, Eileen Mac Sweeney, Andrea Mastinu
Summary: The study found that nitrogen and phosphorus levels significantly impacted the yield and leaf traits of rapeseed. The application of nitrogen and phosphorus together showed the best results in increasing rapeseed yield.
Article
Agronomy
James Anderson, Brant B. Bigger, Kirk Howatt, Joseph Mettler, Marisol T. Berti
Summary: Camelina and canola, both non-transgenic cultivars, showed potential in reducing weed pressure and nutrient leaching in the upper Midwest and northern Great Plains of the USA. Sulfonylurea-resistant camelina and canola reduced late-season dry weight biomass of weeds by >75% and >= 60% respectively compared with fallow plots. However, the application of sulfonylurea herbicides did not significantly alter crop biomass, seed yield, and nutrient retention.
Article
Biochemistry & Molecular Biology
Javier Canales, Jose Verdejo, Gabriela Carrasco-Puga, Francisca M. Castillo, Anita Arenas-M, Daniel F. Calderini
Summary: This study conducted a transcriptomic analysis of the relationship between seed weight and number in two rapeseed genotypes, revealing that a reduction in the source-sink ratio induced the expression of specific gene groups related to sucrose transport, seed weight, and stress responses. Additionally, the research identified co-expression modules correlated with seed weight and negatively correlated with seed number, with a significant enrichment of transcription factors in one module. Network analysis predicted NAC transcription factors as key regulators of seed weight and number compensation in rapeseed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Shan Tang, Ning Guo, Qingqing Tang, Fei Peng, Yunhao Liu, Hui Xia, Shaoping Lu, Liang Guo
Summary: The study highlights the important role of BnaBASS2 in seed lipid metabolism and accumulation in Brassica napus, showing that gene manipulation can alter metabolites in seeds and influence lipid synthesis.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Plant Sciences
Jinyao Yan, Xiaolei Ye, Yi Song, Tao Ren, Chongming Wang, Xiaokun Li, Rihuan Cong, Zhifeng Lu, Jianwei Lu
Summary: This study found that phosphorus (P) and potassium (K) have synergistic effects on crop photosynthesis and productivity. The addition of P gradually removed the limiting factor of P availability and improved photosynthesis. Meanwhile, the addition of K enhances the overall uptake of P and promotes plant growth, mitigating P restrictions on photosynthesis by prioritizing investment in metabolic P fractions.
Article
Biotechnology & Applied Microbiology
Mariana Mamede, Joao Cotas, Kiril Bahcevandziev, Leonel Pereira
Summary: Seaweed polysaccharides have been proven to be more effective than synthetic commercial fertilizers in promoting plant growth. This study extracted polysaccharides from different seaweeds and examined their impact on Brassica napus L. seed germination. Carrageenan showed the best results in seedling growth and germination percentage, which may be attributed to its sulphation level, pH and conductivity.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Agronomy
Xiaohui Zhang, Qiyang Huang, Pengfei Wang, Feiyang Liu, Mudan Luo, Xiang Li, Zhuanrong Wang, Lili Wan, Guangsheng Yang, Dengfeng Hong
Summary: A major QTL for seed weight in rapeseed was fine-mapped, with a 24,482-bp deletion likely playing a crucial role through multiple pathways. Analysis of the candidate causal locus identified important genes controlling seed weight, providing a promising target for breeding higher-yielding rapeseed. The study offers valuable insights into crop yield improvement and seed formation mechanisms in rapeseed.
THEORETICAL AND APPLIED GENETICS
(2021)
Article
Biotechnology & Applied Microbiology
Zhongchun Xiao, Fang Tang, Liyuan Zhang, Shengting Li, Shufeng Wang, Qiang Huo, Bo Yang, Chao Zhang, Daojie Wang, Qing Li, Lijuan Wei, Tao Guo, Cunmin Qu, Kun Lu, Yanfeng Zhang, Liang Guo, Jiana Li, Nannan Li
Summary: The study on the FAX1-1 gene in rapeseed seeds has the potential to improve seed oil content, oil quality, and biological yield simultaneously, providing new approaches for future molecular breeding.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Plant Sciences
Zheng-Jun Guan, Min Zheng, Zhi-Xi Tang, Wei Wei, C. Neal Stewart Jr
Summary: In this study, differential expression of total protein in leaves of two transgenic oilseed rape lines and their non-transgenic parent plant was analyzed. Fourteen differential protein spots were identified, including eleven upregulated and three downregulated proteins, involved in photosynthesis, transporter function, metabolism, protein synthesis, and cell growth and differentiation. These changes in protein spots may be attributed to the insertion of foreign transgenes in transgenic oilseed rape, but transgenic manipulation does not necessarily cause significant changes in the proteomes of oilseed rape.
Article
Agronomy
Maoyan Zou, Taoxiong Shi, Wei Wang, Guangda Ding, Fangsen Xu, Lei Shi
Summary: This study investigated the genetic mechanism of seed yield and related traits in oilseed rape (B. napus) in response to nitrogen deficiency. Significant quantitative trait loci (QTLs) were detected for plant height, branch number, pod number, seed number, 1000-seed weight, and seed yield under optimal and low nitrogen conditions. Stable QTLs were integrated from multiple trials, and consensus QTLs and unique QTLs were obtained. Epistatic interactions affecting seed yield were identified. These findings provide insights into the genetic basis of yield traits under low nitrogen conditions and facilitate the breeding of nitrogen-efficient varieties.
MOLECULAR BREEDING
(2022)
Article
Biochemical Research Methods
Masoud Shahsavari, Valiollah Mohammadi, Bahram Alizadeh, Houshang Alizadeh
Summary: This study aims to enhance the efficiency of indirect selection for rapeseed seed yield (SY) by combining machine learning algorithms and feature selection methods. The results indicate that a combination of multilayer perceptron neural network algorithm with stepwise and backward selection methods can accurately predict the SY of rapeseed.
Article
Agronomy
Haijiang Liu, Maoyan Zou, Bingbing Zhang, Xinyu Yang, Pan Yuan, Guangda Ding, Fangsen Xu, Lei Shi
Summary: This study conducted a genome-wide association study on the genetic mechanisms of seed yield in oilseed rape, and identified several significant SNPs associated with seed yield. Two candidate genes were identified through transcriptome and candidate gene analysis. These results provide valuable information and resources for understanding the genetic control of seed yield in oilseed rape and breeding high-yield cultivars.
MOLECULAR BREEDING
(2022)
Article
Plant Sciences
Han Liu, Baogang Lin, Yun Ren, Pengfei Hao, Lan Huang, Bowen Xue, Lixi Jiang, Yang Zhu, Shuijin Hua
Summary: In this study, a precise genome editing method was established to edit the A5 and C5 loci of BnFAD2 in rapeseed, resulting in a significant increase in seed oleic acid content. The mutants showed a relative proportion of oleic acid greater than 85% in both the T-1 and T-4 generations, indicating a successful editing of the targeted genes. There were no significant differences in seed yield and oil content between the mutants and the wild type, but the mutants exhibited a markedly higher relative proportion of oleic acid.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Lei Hua, Sean R. Stevenson, Ivan Reyna-Llorens, Haiyan Xiong, Stanislav Kopriva, Julian M. Hibberd
Summary: The gene expression patterns in the bundle sheath and mesophyll cells of rice show specialized functions in water transport, sulphur assimilation, and jasmonic acid biosynthesis. Despite the small chloroplast compartment in bundle sheath cells, there is significant photosynthesis gene expression. These patterns are not linked to specific transcription factors, but instead are associated with gradients across the leaf. Comparisons with Arabidopsis suggest the conservation of gene expression patterns in bundle sheath cells across species.
Article
Plant Sciences
Sandra E. Zimmermann, Ruben M. Benstein, Maria Flores-Tornero, Samira Blau, Armand D. Anoman, Sara Rosa-Tellez, Silke C. Gerlich, Mohamed A. Salem, Saleh Alseekh, Stanislav Kopriva, Vera Wewer, Ulf-Ingo Fluegge, Richard P. Jacoby, Alisdair R. Fernie, Patrick Giavalisco, Roc Ros, Stephan Krueger
Summary: The study reveals the crucial role of the phosphorylated pathway of serine biosynthesis (PPSB) in plant growth and metabolism, with deficiency in PPSB leading to nitrogen assimilation induction.
Article
Biotechnology & Applied Microbiology
Mahnaz Nezamivand-Chegini, Esmaeil Ebrahimie, Ahmad Tahmasebi, Ali Moghadam, Saeid Eshghi, Manijeh Mohammadi-Dehchesmeh, Stanislav Kopriva, Ali Niazi
Summary: The study analyzed the evolution of the SPX gene family in legumes and other species, revealing fluctuations in different classes of SPX proteins in different plants, as well as the sub- or neo-functionalization of duplicated genes in legumes. Functional orthologs in soybean and Medicago were identified using two different methods.
Article
Biochemistry & Molecular Biology
Aurelien D'Oria, Lun Jing, Mustapha Arkoun, Sylvain Pluchon, Stephanie Pateyron, Jacques Trouverie, Philippe Etienne, Sylvain Diquelou, Alain Ourry
Summary: The study showed that even in mild water deficit, ion transport was one of the most significantly affected processes, supported by changes in ion composition occurring before growth reduction. Metabolomic and most of the transcriptomic data indicated that well-known early leaf responses to drought, such as phytohormone metabolism, proline accumulation, and oxidative stress defense, were induced later than repression of genes related to nutrient transport.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Natallia Ashykhmina, Kai Xun Chan, Henning Frerigmann, Frank Van Breusegem, Stanislav Kopriva, Ulf-Ingo Fluegge, Tamara Gigolashvili
Summary: Plants have highly compartmentalized eukaryotic cells, with plastids and mitochondria relying on the flow of information to and from the nuclei. Retrograde and anterograde signals, such as the retrograde signaling molecule PAP, play a role in coordinating intracellular functions in response to biotic and abiotic stresses. The SAL1 protein, which dephosphorylates PAP to AMP, has different stability and impacts in different cell compartments. Further research is needed to understand the role of the SAL1-PAP pathway in various cellular processes under stress conditions.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Plant Sciences
Anna Koprivova, Stanislav Kopriva
Summary: Plants communicate with microorganisms through metabolites exuded from roots, which play a crucial role in modulating the functions of plant associated microbiota. Recent research has identified various metabolites involved in this communication network, but there are still significant knowledge gaps to be filled in future studies.
CURRENT OPINION IN PLANT BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ludmila Maiber, Anna Koprivova, Daniel Bender, Stanislav Kopriva, Katrin Fischer-Schrader
Summary: The roles of ARC1 and ARC2 in NO production in higher plants are different, but their specific physiological functions remain unclear.
Article
Plant Sciences
M. Shamim Hasan, Divykriti Chopra, Anika Damm, Anna Koprivova, Stanislav Kopriva, Andreas J. Meyer, Stefanie Mueller-Schuessele, Florian M. W. Grundler, Shahid Siddique
Summary: Cyst nematodes are damaging parasitic organisms that infect crop plants, and this study reveals the role of glutathione in plant resistance to cyst nematode infection. Glutathione levels were found to influence plant susceptibility to the nematode, possibly by regulating the balance between cellular redox environment and the production of compounds related to defense against infection.
MOLECULAR PLANT PATHOLOGY
(2022)
Review
Multidisciplinary Sciences
Daniela Ristova, Stanislav Kopriva
Summary: As sessile organisms, plants have developed mechanisms to sense and utilize nutrients from the environment and coordinate their growth and development accordingly. This research highlights the complex regulation of nutrient assimilation and the importance of sulfur in comparison to other macronutrients such as nitrogen and phosphorus. The study also focuses on sulfur sensing and signaling, uptake and assimilation, and highlights the core genes regulated by the transcriptional factor SLIM1/EIL3 in response to sulfur deficiency.
Article
Plant Sciences
Jaroslav Pavlu, Pavel Kerchev, Martin Cerny, Jan Novak, Miroslav Berka, Timothy O. Jobe, Jose Maria Lopez Ramos, Inigo Saiz-Fernandez, Aaron Michael Rashotte, Stanislav Kopriva, Bretislav Brzobohaty
Summary: Cytokinins have an interplay with sulfur homeostasis and are involved in regulating the expression of sulfur-responsive genes and levels of sulfate and glutathione. The cytokinin receptor ARABIDOPSIS HISTIDINE KINASE 3 and cytokinin-deficient plants play a role in glutathione accumulation. On the other hand, cytokinin also triggers sulfur starvation-like gene expression and decreases sulfate and glutathione content.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Parisa Rahimzadeh Karvansara, Ciaran Kelly, Raissa Krone, Ivan Zenzen, Daniela Ristova, Emely Silz, Timothy O. Jobe, Stanislav Kopriva
Summary: The regulatory mechanisms of sulfate assimilation in rice and Setaria viridis are different from Arabidopsis, with both species being capable of oxidizing cysteine to sulfate.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Anna Koprivova, Melina Schwier, Vanessa Volz, Stanislav Kopriva
Summary: Plants release secondary metabolites from the roots to shape their microbiome, with many of these compounds having antimicrobial activities and playing a role in plant immunity. The study focused on the dynamics of camalexin synthesis and exudation in Arabidopsis thaliana when interacting with the plant growth promoting bacteria Pseudomonas sp. CH267 or the bacterial pathogen Burkholderia glumae PG1. The results show that camalexin accumulation and exudation are induced more rapidly but transiently by the growth promoting bacteria, while the pathogen induces higher and more stable levels of camalexin. Furthermore, camalexin synthesis is controlled on a whole plant level and coordinated between the shoots and the roots, where exuded camalexin originates in the shoots.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Anna Koprivova, Buesra Elkatmis, Silke C. Gerlich, Martin Trick, Andrea L. Harper, Ian Bancroft, Stanislav Kopriva
Summary: Sulfur is crucial for plant metabolism and is involved in many different compounds. However, there is limited knowledge about sulfur sensing and signaling, as well as mechanisms controlling sulfur metabolism and homeostasis. Variations in sulfur content exist not only among different plant species, but also among accessions of the same species. In Arabidopsis thaliana, loss of the OASC gene reduces sulfate and glutathione levels in the leaves due to lower sulfate uptake and translocation. A single nucleotide polymorphism in the OASC gene is linked to variation in sulfate content. The mitochondrial isoform of OAS-TL is the next metabolic enzyme with a role in regulating sulfate content in Arabidopsis, after sulfurylase and APS reductase 2.
Editorial Material
Plant Sciences
Titouan Bonnot, Fanelie Bachelet, Julie Boudet, Christine Le Signor, Emmanuelle Bancel, Vanessa Vernoud, Catherine Ravel, Karine Gallardo
Summary: Improving seed protein quality by stabilizing sulfur nutrition is of great importance for food and agroecological transitions. Sulfur is crucial for the synthesis of sulfur-rich proteins in seeds, and its deficiency negatively impacts protein composition, leading to health problems in humans. Sulfur also plays a key role in stress tolerance. Recent studies have made significant advances in understanding the interaction between sulfur nutrition and environmental or nutritional stresses, metabolic pathways to mitigate stress impacts on seed protein quality, and the regulation of seed protein composition through sulfur homeostasis.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Nicholas de Jager, Varsa Shukla, Anna Koprivova, Martin Lycka, Lorina Bilalli, Yanrong You, Juergen Zeier, Stanislav Kopriva, Daniela Ristova
Summary: Sulfur is an essential mineral nutrient for plant growth and development. Its content in leaves is tightly connected with sulfate uptake, concentration of sulfate and phosphate anions, and synthesis of glucosinolates and glutathione. Sulfur homeostasis in Arabidopsis thaliana is regulated by biotic and abiotic stressors, leading to large variation in sulfur content among different accessions.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)