Article
Immunology
Guillaume Padern, Claire Duflos, Rosanna Ferreira, Said Assou, Philippe Guilpain, Alexandre Thibault Jacques Maria, Radjiv Goulabchand, Pascale Galea, Maja Jurtela, Christian Jorgensen, Yves-Marie Pers
Summary: This exploratory study investigated serum biomarkers that may distinguish primary Sjogren's syndrome (pSS) from rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). Results showed that certain biomarkers such as BDNF and I-TAC/CXCL11 can significantly discriminate pSS from RA, while other biomarkers like sCD163, Fractalkine/CX3CL1, MCP-1/CCL2, and TNFa were associated with SLE diagnosis compared to pSS. Combinations of these biomarkers showed high specificity and positive predictive value for distinguishing between pSS, RA, and SLE.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Theophilos Tzaridis, Johannes Weller, Daniel Bachurski, Farhad Shakeri, Christina Schaub, Peter Hau, Andreas Buness, Uwe Schlegel, Joachim-Peter Steinbach, Clemens Seidel, Roland Goldbrunner, Niklas Schaefer, Robert J. Wechsler-Reya, Michael Hallek, Bjoern Scheffler, Martin Glas, Lothar Haeberle, Ulrich Herrlinger, Christoph Coch, Katrin S. Reiners, Gunther Hartmann
Summary: Evaluation of serum-derived extracellular vesicles (EVs) from glioblastoma patients reveals that the upregulation of CD29, CD44, CD81, C1QA, and histone H3 in serum EVs correlates with tumor progression, suggesting their potential as biomarkers.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Parasitology
Nathalia Anderson Calomeno, Renato Simoes Moreira, Leonardo Antonio Fernandes, Franciane Batista, Julia Marques, Glauber Wagner, Luiz Claudio Miletti
Summary: The study conducted a comprehensive analysis of the serum proteome of mice infected with Trypanosoma evansi using LC-MS/MS, identifying 30 T. evansi proteins and 31 mouse proteins with abundance changes. Two exclusive VSG epitopes were also identified in the host serum.
VETERINARY PARASITOLOGY
(2021)
Article
Oncology
Ammara Muazzam, Matt Spick, Olivier N. F. Cexus, Bethany Geary, Fowz Azhar, Hardev Pandha, Agnieszka Michael, Rachel Reed, Sarah Lennon, Lee A. Gethings, Robert S. Plumb, Anthony D. Whetton, Nophar Geifman, Paul A. Townsend
Summary: Despite ongoing research, effective tools for detection and monitoring of prostate cancer are still lacking. This study aims to identify non-invasive biomarkers that can complement PSA in clinical decision-making. Novel prostate cancer protein signatures were discovered using an advanced analytical technique, and the results were validated using a second cohort of patients. The study provides a proteomic signature that can enhance the diagnosis and risk assessment of localized prostate cancer.
Article
Cardiac & Cardiovascular Systems
Dongmei Wei, Sander Trenson, Jan M. Van Keer, Jesus Melgarejo, Ella Cutsforth, Lutgarde Thijs, Tianlin He, Agnieszka Latosinska, Agnieszka Ciarka, Thomas Vanassche, Lucas Van Aelst, Stefan Janssens, Johan Van Cleemput, Harald Mischak, Jan A. Staessen, Peter Verhamme, Zhen-Yu Zhang
Summary: This study identified and validated a urinary proteomic signature for the surveillance of cardiac allograft vasculopathy (CAV). The proteins in the signature may offer insights into CAV pathological processes and warrant further investigation into personalized treatment targets.
Article
Cell Biology
Shuang-shuang Gu, Jin Li, Min Jiang, Yi Zhou, Bing Yang, Kehui Xie, Yun-fei Jiang, Xin-rui Jiang, Fei He, Jun Wang
Summary: By analyzing serum proteomics, 38 up-regulated proteins and 16 down-regulated proteins related to neurological recovery were identified in patients after cardiac arrest. Serum AGT and SERPINA1 were confirmed to be associated with neurological function and prognosis. The established prognostic risk score can assist in adjusting current therapeutic strategies.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Indu Sinha, Rachel L. Fogle, Gizem Gulfidan, Anne E. Stanley, Vonn Walter, Christopher S. Hollenbeak, Kazim Y. Arga, Raghu Sinha
Summary: Breast cancer is a leading cause of death in women in the US, and current diagnostic methods have high false positivity rates. This study aimed to identify protein markers in saliva and serum for early breast cancer detection. Through rigorous analysis using iTRAQ technique, a total of 591 and 371 differentially expressed proteins were identified in saliva and serum samples, respectively. These proteins were mainly related to exocytosis, secretion, immune response, and signaling pathways. A network biology approach was used to evaluate potential biomarkers for breast cancer diagnosis and prognosis. The findings suggest that saliva and serum samples from the same women can provide a feasible platform for studying responsive proteomic profiles in benign and malignant breast disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Gregorio Guilherme Almeida, Inga Rimkute, Isabela Natalia Pascoal Campos Do Vale, Thomas Liechti, Priscilla Miranda Henriques, Ester Roffe, Fernanda Fortes de Araujo, Manoel Otavio da Costa Rocha, Silvana Maria Eloi Santos, Olindo Assis Martins-Filho, Dragana Jankovic, Alan Sher, Andrea Teixeira-Carvalho, Mario Roederer, Lis Ribeiro do Valle Antonelli
Summary: In this study, high-dimensional flow cytometry was used to evaluate CD4(+) T cells in patients with different clinical forms. The results showed that activated CD4(+) T cells were increased in patients, while functional regulatory T cells were reduced. These findings provide evidence for the involvement of these cells in the development of Chagas cardiomyopathy and their potential as biomarkers for disease progression.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Rania Dagher, Paul Fogel, Jingya Wang, David Soussan, Chia-Chien Chiang, Jennifer Kearley, Daniel Muthas, Camille Taille, Patrick Berger, Arnaud Bourdin, Cecile Chenivesse, Sylvie Leroy, Gary Anderson, Alison A. Humbles, Michel Aubier, Roland Kolbeck, Marina Pretolani
Summary: This study investigates novel biomarkers related to clinical hallmarks of COPD using proteomic analysis. Two clusters of COPD patients were identified, with one cluster showing elevated levels of factors contributing to tissue injury, and the other cluster showing higher expression of proteins associated with enhanced immunity, host defense, cell fate, remodeling and repair, and altered metabolism/mitochondrial functions. These protein expression patterns were consistent during follow-up visits and in a limited series of COPD patients. A signature of 15 proteins accurately differentiated the two COPD clusters. This study provides insights into COPD heterogeneity and suggests the potential for targeted therapies based on specific COPD endotypes.
Article
Pharmacology & Pharmacy
Rui Zeng, Tian-Cheng Ke, Mao-Ta Ou, Li-Liang Duan, Yi Li, Zhi-Jing Chen, Zhi-Bin Xing, Xiao-Chen Fu, Cheng-Yu Huang, Jing Wang
Summary: By analyzing gene expression data, we established a transcriptome diagnostic signature that can predict the risk of postmenopausal osteoporosis and has potential clinical application and drug candidate selection value.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Pawel Gutaj, Jan Matysiak, Eliza Matuszewska, Katarzyna Jaskiewicz, Dorota Kaminska, Agata Swiatly-Blaszkiewicz, Tomasz Szczapa, Anastasia Kalantarova, Marzena Gajecka, Ewa Wender-Ozegowska
Summary: This study analyzed serum proteomic profiling in pregnant women with T1DM in the late third trimester and identified distinct protein differences in the coagulation and inflammatory pathways. However, further investigation is needed to determine the utility of these differential proteins as biomarkers for pregnancy complications in women with T1DM.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Denise S. Uyar, Yi-Wen Huang, Marla A. Chesnik, Ninh B. Doan, Shama P. Mirza
Summary: This study aimed to identify unique biomarkers of early stage, low-grade endometrial cancer through comprehensive proteomic analysis of patient serum, with FAM83D identified as a potential biomarker. The overexpression of FAM83D in early endometrial cancer samples suggests its significance as a target for therapies in a disease with few biomarkers.
JOURNAL OF PROTEOMICS
(2021)
Article
Biochemistry & Molecular Biology
Ana Filipa Fernandes, Luis Gafeira Goncalves, Maria Bento, Sandra Anjo, Bruno Manadas, Clara Barroso, Miguel Villar, Rita Macedo, Maria Joao Simoes, Ana Varela Coelho
Summary: This study aimed to discover molecular biomarkers for the early diagnosis of tuberculosis. Proteomics and metabolomics analyses were performed, and a combination of protein and metabolite markers was found to accurately identify tuberculosis patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Alireza Delfarah, Nicolas G. Hartel, DongQing Zheng, Jesse Yang, Nicholas A. Graham
Summary: Senescence is a permanent cell cycle arrest in response to cellular stress, with diverse effects depending on cell type and inducer. Analyzing primary human mammary epithelial cells using mass spectrometry-based proteomics revealed a robust proteomic signature of senescence. The study identified known and novel senescence biomarkers, and demonstrated upregulation of lysosomal proteins and downregulation of RNA metabolic processes in senescent cells. The results highlight the power of proteomics in identifying cell type-specific senescence signatures and enhancing understanding of senescence in HMECs.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Mengxue Zhang, Xiao Chen, He Chen, Minyue Zhou, Yaoqiang Liu, Yali Hou, Minhai Nie, Xuqian Liu
Summary: The study identified 6 potential biomarkers for the diagnosis of oral squamous cell carcinoma (OSCC) using protein microarray assay, with GDF15 as a prognostic indicator. Experimental results showed significantly higher expression levels of these proteins in cancer cells compared to normal cells, and upregulated expression in cancer tissues.
Article
Microbiology
Jake Jianjun Wen, Yuhui Whitney Yin, Nisha Jain Garg
Article
Infectious Diseases
Jake J. Wen, Nisha Jain Garg
PLOS NEGLECTED TROPICAL DISEASES
(2018)
Article
Surgery
Jake J. Wen, Claire B. Cummins, Bartosz Szczesny, Ravi S. Radhakrishnan
JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS
(2020)
Article
Biochemistry & Molecular Biology
Jake J. Wen, Claire B. Cummins, Ravi S. Radhakrishnan
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Cell Biology
Jake J. Wen, Claire Cummins, Ravi S. Radhakrishnan
Article
Biochemistry & Molecular Biology
Jake J. Wen, Claire B. Cummins, Taylor P. Williams, Ravi S. Radhakrishnan
Review
Biochemistry & Molecular Biology
Yu Xiao, Mengjuan Li, Xiaoyi Guo, Hui Zeng, Xuehong Shuai, Jianhua Guo, Qingzhou Huang, Yuefeng Chu, Bo Zhou, Jake Wen, Jun Liu, Hanwei Jiao
Summary: Brucellosis is a severe zoonotic infectious disease that causes considerable economic losses. The mechanism of placentitis or abortion caused by Brucella infection in livestock is still not fully understood and requires further research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Health Care Sciences & Services
Jana E. DeJesus, Jake J. Wen, Ravi Radhakrishnan
Summary: Burn injuries can cause persistent inflammatory response, leading to cardiac dysfunction and changes in gene expression. The release of inflammatory mediators and the role of cytokines are the main mechanisms underlying these issues.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
Jake J. Wen, Geetha L. Radhakrishnan, Craig Porter, Ravi S. Radhakrishnan
CIRCULATION RESEARCH
(2019)
Meeting Abstract
Surgery
Claire Cummins, Xiaofu Wang, Jake J. Wen, Ravi Radhakrishnan
JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS
(2019)
Meeting Abstract
Critical Care Medicine
Xiaofu Wang, Claire B. Cummins, Jake J. Wen, Celeste C. Finnerty, David N. Herndon, Jia Zhou, Xiang Fang, Ravi Radhakrishnan
Meeting Abstract
Critical Care Medicine
Jake J. Wen, Jake J. Wen, Claire B. Cummins, Xiaofu Wang, David N. Herndon
Meeting Abstract
Cardiac & Cardiovascular Systems
Jake J. Wen, Nisha J. Garg
CIRCULATION RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Jake Jianjun Wen, Craig Porter, Nisha Jain Garg
ANTIOXIDANTS & REDOX SIGNALING
(2017)
Meeting Abstract
Critical Care Medicine
Jake J. Wen, Geetha L. Radhakrishnan, Claire B. Cummins, Ravi S. Radhakrishnan