Article
Chemistry, Multidisciplinary
Xiangyu Guo, Wenbo Cao, Xiaomin Fan, Zhiying Guo, Donghui Zhang, Haoyue Zhang, Xiaoxiao Ma, Jiahong Dong, Yunfang Wang, Wenpeng Zhang, Zheng Ouyang
Summary: Mass spectrometry imaging (MSI) of lipids in biological tissues can provide valuable information for biological research and disease diagnosis by correlating molecular distribution with pathological results. A new multiplexed MS2 imaging method (MSI-I-2) has been developed, allowing for MS2 images of 10 or more lipid species at each sampling spot, providing spatial structural lipidomic information. Coupled with on-tissue photochemical derivatization, imaging of 20 phospholipid C=C location isomers is also possible, resulting in enhanced molecular images with high structural definition for mouse brain and human liver cancer tissue sections. Spatially mapped t-distributed stochastic neighbor embedding has been utilized to visualize the tumor margin with the assistance of structural lipidomic information.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Aron Bartha, Zsuzsanna Darula, Gyoengyi Munkacsy, Eva Klement, Peter Nyirady, Balazs Gyorffy
Summary: This research used a proteotranscriptomic approach to differentiate normal and tumor tissues in clear cell renal cell carcinoma (ccRCC). Top genes over-expressed in ccRCC were identified using transcriptomic data of patients with malignant and normal tissue samples. Protein level analysis and mass spectrometry further validated the differential protein abundance of these genes. A minimal panel of proteins highly specific for ccRCC tissues was identified using transcriptomic and proteomic data.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Louis J. Sparvero, Hua Tian, Andrew A. Amoscato, Wan-Yang Sun, Tamil S. Anthonymuthu, Yulia Y. Tyurina, Oleksandr Kapralov, Sabzali Javadov, Rong-Rong He, Simon C. Watkins, Nicholas Winograd, Valerian E. Kagan, Hulya Bayir
Summary: Using gas cluster ion beam secondary ion mass spectrometry (GCIB-SIMS) imaging, peroxidized phosphatidylethanolamine (PEox) could be mapped with high spatial resolution in ferroptotic cardiomyocytes and neurons after traumatic brain injury, revealing their accumulation at very low physiological levels in subcellular compartments.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Biochemistry & Molecular Biology
Rashid Ahmed, Robin Augustine, Enrique Valera, Anurup Ganguli, Nasrin Mesaeli, Irfan S. Ahmad, Rashid Bashir, Anwarul Hasan
Summary: Spatial mapping of gene expression in cancer tissues can enhance our understanding of cancers and improve cancer detection accuracy. Recent advancements in OMICS technologies have the potential to map biopsy tissue samples and their molecular profiles at a single-cell level. However, further developments are needed for the widespread and effective application of these technologies.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2022)
Article
Chemistry, Analytical
Marjan Dolatmoradi, Sylwia A. Stopka, Chloe Corning, Gary Stacey, Akos Vertes
Summary: In this study, a mass spectrometry enhanced with ion mobility separation and coupled with an automated sampling platform was utilized for in situ high-throughput single-cell metabolomics in soybean root nodules. The system achieved a high sampling rate and improved molecular coverage, leading to the discovery of metabolic heterogeneity. The upgraded platform allowed for the characterization of both unimodal and bimodal metabolite abundance distributions, revealing the presence of distinct subpopulations.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Anthony Maus, Adam Girtman, Jessa Kiesling, Jennifer Faber, Stefan K. G. Grebe
Summary: Pyridoxal-5'-phosphate (PLP), the active form of vitamin B6, plays a crucial role in enzymatic reactions. Accurate measurement of PLP is essential for diagnoses and monitoring, but traditional assays are prone to interferences. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) can overcome these issues, and adding an ion pairing reagent like 1-octanesulfonic acid (OSA) after extraction greatly improves the signal-to-noise ratio and precision of measurements without harming the instrument hardware commonly associated with traditional ion pairing reagent techniques.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Review
Chemistry, Analytical
Hanne Roberg-Larsen, Elsa Lundanes, Tuula A. Nyman, Frode S. Berven, Steven Ray Wilson
Summary: Single-cell analysis provides in-depth insights into diseases and diagnostics, with liquid chromatography playing a crucial role. The development of novel sample preparation techniques and improvements in mass spectrometry are enhancing the promise of single-cell analysis. However, technical challenges and the need for higher throughput and robustness may lead to the reinvention of alternative nano LC column formats.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biotechnology & Applied Microbiology
Marco Vicari, Reza Mirzazadeh, Anna Nilsson, Reza Shariatgorji, Patrik Bjaerterot, Ludvig Larsson, Hower Lee, Mats Nilsson, Julia Foyer, Markus Ekvall, Paulo Czarnewski, Xiaoqun Zhang, Per Svenningsson, Lukas Kaell, Per E. Andren, Joakim Lundeberg
Summary: We present a method that combines histology, mass spectrometry imaging, and spatial transcriptomics to measure mRNA transcripts and low-molecular-weight metabolites simultaneously across tissue regions. The potential of this method is demonstrated using mouse and human brain samples in the context of dopamine and Parkinson's disease.
NATURE BIOTECHNOLOGY
(2023)
Review
Immunology
Laura A. Solt
Summary: T cells transition from a quiescent state to active proliferation and effector function upon exposure to cognate antigen, a process tightly controlled by signal transduction pathways. Dysregulation of these events can mediate disease, and recent sequencing advances at the single-cell level have improved our understanding of T cell biology.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Social Issues
Rajenlall Siriram
Summary: An innovation management investigative framework (IMIF) is developed to examine the relationship between drivers of technological change and firm performance. The study finds that factors such as effectuation, entrepreneurial orientation, strategy and leadership, contribute to overcoming barriers to technological change and improving firm's competitive position and performance. The research supports the proposed framework and suggests further research opportunities.
TECHNOLOGY IN SOCIETY
(2022)
Review
Endocrinology & Metabolism
Jacquelien J. Hillebrand, Wjera Wickenhagen, Annemieke C. Heijboer
Summary: Despite little attention paid to hormone analyses in research studies, it is crucial to consider the quality of hormone measurements to prevent false conclusions and ensure appropriate follow-up studies. Various factors such as technique quality, expertise, matrices, timing and storage conditions, freeze-thaw cycles, variation, and standardization should be considered when conducting scientific research involving hormone measurements. This article aims to clarify the importance of paying attention to hormone analyses and provide guidance for decision-making in research studies.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Review
Chemistry, Physical
Danish Khan, Geping Qu, Imran Muhammad, Zeguo Tang, Zong-Xiang Xu
Summary: This article provides an overview of the development of monolithic perovskite-silicon tandem solar cells and discusses the major challenges faced in achieving efficient, stable, and scalable devices. The relationship between perovskite band gap and silicon texture is crucial but currently unclear due to conflicting research outcomes.
ADVANCED ENERGY MATERIALS
(2023)
Review
Chemistry, Analytical
Caitlin E. Randolph, Palak Manchanda, Harshit Arora, Sanjay Iyer, Pooja Saklani, Connor Beveridge, Gaurav Chopra
Summary: Lipidomics, as a standalone subdiscipline of metabolomics, has received considerable attention in the past decade. Mass spectrometry has emerged as the preferred tool for lipid identification and detection due to its sensitivity and versatility. However, traditional lipidomics methods based on bulk cell samples do not capture the cellular heterogeneity present in seemingly homogeneous cell populations. This review focuses on recent advances in mass spectrometry-based single cell lipidomics and explores the reasons behind the slow development of single-cell lipidomics techniques.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Sean W. W. Huth, James V. V. Oakley, Ciaran P. P. Seath, Jacob B. B. Geri, Aaron D. D. Trowbridge, Dann L. L. Parker Jr, Frances P. P. Rodriguez-Rivera, Adam G. G. Schwaid, Carlo Ramil, Keun Ah Ryu, Cory H. H. White, Olugbeminiyi O. O. Fadeyi, Rob C. C. Oslund, David W. C. MacMillan
Summary: Characterization of ligand binding modes is crucial in drug discovery, especially in campaigns resulting from phenotypic screening. X-ray crystallography and photoaffinity labeling (PAL) are commonly used methods, but they have limitations. We have developed a photocatalytic approach to map protein binding sites, achieving identification of residues near the ligand binding pocket. This method has been successfully used to map multiple protein targets and investigate the binding site of a ligand with no known crystal structure, both in vitro and in live cells.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Suprama Datta, Da-Yuan Chen, Alexander H. Tavares, Tamara Reyes-Robles, Keun Ah Ryu, Nazimuddin Khan, Tyler J. Bechtel, Jayde M. Bertoch, Cory H. White, Daria J. Hazuda, Kalpit A. Vora, Erik C. Hett, Olugbeminiyi O. Fadeyi, Rob C. Oslund, Andrew Emili, Mohsan Saeed
Summary: Identifying virus-host interactions on the cell surface is crucial for understanding viral entry and pathogenesis. Researchers have developed a platform called ViraMap, which utilizes photocatalysis to study the molecular neighborhood of the SARS-CoV-2 spike protein on human cell surfaces. This study has revealed novel cell surface proteins that contribute to viral entry, providing valuable insights into SARS-CoV-2 infection.
CELL CHEMICAL BIOLOGY
(2023)