Article
Biochemistry & Molecular Biology
Xiangyuan Li, Lei Fu, Shan Zhang, Yipeng Wang, Lianghui Gao
Summary: This study used molecular dynamics simulations to evaluate the damaging effects of sequence-truncated and residue-substituted variants of Alligator sinensis cathelicidins on the membranes of Gram-negative bacteria. The simulations predicted that these variants can disrupt the structures of the inner membrane of Gram-negative bacteria, but not the outer membrane. AS4-9, in particular, showed the highest antibacterial activity and was able to extract lipids from the membrane. These findings have the potential to inspire the development of potent therapeutic agents targeting bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Qingliang Wang, Xiaojie Li, Wenting Tang, Xiaoling Guan, Zhiyong Xiong, Yong Zhu, Jiao Gong, Bo Hu
Summary: This study explored the gene differences between Gram-negative, Gram-positive, and mixed infections in sepsis using genome-wide techniques. The results showed molecular heterogeneity between Gram-negative and Gram-positive sepsis, which was confirmed in the validation dataset.共有40个共同的差异基因集识别. The findings offer new insights into investigating the pathophysiology of bacterial sepsis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Applied
M. S. Gualdesi, J. Vara, V Aiassa, C. I. Alvarez Igarzabal, C. S. Ortiz
Summary: The study focused on developing third generation photosensitizers using PAA-NPs for antimicrobial photodynamic therapy. These new photosensitizers exhibited improved photochemical reactivity and photodynamic efficacy, showing potential applications against Gram-positive and Gram-negative bacteria.
Article
Multidisciplinary Sciences
Kumar Sachin Singh, Rishabh Sharma, Poli Adi Narayana Reddy, Prashanthi Vonteddu, Madeline Good, Anjana Sundarrajan, Hyeree Choi, Kar Muthumani, Andrew Kossenkov, Aaron R. Goldman, Hsin-Yao Tang, Maxim Totrov, Joel Cassel, Maureen E. Murphy, Rajasekharan Somasundaram, Meenhard Herlyn, Joseph M. Salvino, Farokh Dotiwala
Summary: Isoprenoids are essential for maintaining cellular functions and IspH is a crucial enzyme in their synthesis pathway. Through structure-guided design, a class of IspH inhibitors with nanomolar potency were identified, showing efficacy against multidrug-resistant bacteria while being relatively non-toxic to mammalian cells. These prodrugs also induce expansion and activation of human V gamma 9V delta 2 T cells, suggesting a potential strategy to limit antibiotic-resistant bacterial populations.
Review
Cell Biology
He Liu, Zhen Hu, Mengyang Li, Yi Yang, Shuguang Lu, Xiancai Rao
Summary: G(+) bacterial infections pose a significant burden and require urgent development of antimicrobial agents due to the prevalence of multidrug-resistant G(+) bacteria. Endolysins, phage-encoded enzymes, are promising alternatives for combating resistance due to their low bacterial resistance. This review classifies and summarizes the mechanisms, efficacy, and advantages of endolysins as antibacterial drug candidates targeting G(+) bacteria. Safety, challenges, and potential solutions are also addressed, with indications that endolysin-based drugs will be approved in the near future.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Article
Immunology
Stephanie Shulder, Pranita D. Tamma, Suiyini Fiawoo, Kathryn Dzintars, Daniel Escobar, Daniel J. Livorsi, Anurag N. Malani, Danica Palacio, Emily S. Spivak, Matty Zimmerman, Jacqueline T. Bork
Summary: A 24-site observational cohort study showed that infectious diseases consultation (IDC) reduced the risk of 30-day mortality by 40% in hospitalized patients with Gram-negative bacteremia (GN-BSI).
CLINICAL INFECTIOUS DISEASES
(2023)
Review
Immunology
Jing Zhang, Ling Chen, Yang Yang, Xiao Liu, Yuan Yuan, Shi-rong Song, Yue Zhao, Jian Mao
Summary: The purpose of this study was to compare the clinical and laboratory characteristics of late-onset sepsis (LOS) caused by Gram-negative and Gram-positive bacteria in preterm neonates and evaluate the diagnostic accuracy of biomarkers. The results showed that LOS caused by Gram-negative bacteria in preterm neonates had higher levels of CRP and lower platelet counts. Therefore, platelet count and CRP values may be useful biomarkers to differentiate sepsis caused by Gram-negative and Gram-positive bacteria in preterm neonates.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Microbiology
Arianne Lovey, Mila Krel, Allen Borchardt, Thomas Brady, Jason N. Cole, Quyen-Quyen Do, Joanne Fortier, Grayson Hough, Wanlong Jiang, Alain Noncovich, Les Tari, Qiping Zhao, James M. Balkovec, Yanan Zhao, David S. Perlin
Summary: ADCs have the potential to bind strongly to drug-resistant G- pathogens, regardless of lipopolysaccharide modifications, indicating a promising treatment alternative in infections caused by drug-resistant G- bacteria.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Antonella Migliaccio, Maria Stabile, Maria Bagattini, Maria Triassi, Rita Berisio, Eliana De Gregorio, Raffaele Zarrilli
Summary: Resveratrol can reverse tolerance and restore susceptibility to CHX and BZK in the majority of microorganisms causing HAI. The efflux pump inhibitor reduced the MIC and MBC of CHX and BZK in most strains, while resveratrol dose-dependently decreased the MIC and MBC of the biocides.
Article
Immunology
Cynthia Mubanga, Michelo Simuyandi, Kapambwe Mwape, Kennedy Chibesa, Caroline Chisenga, Obvious Nchimunya Chilyabanyama, Arlo Randall, Xiaowu Liang, Richard H. Glashoff, Roma Chilengi
Summary: Developing a broadly protective vaccine against most variants of ETEC has been difficult. The most promising candidate so far is an oral inactivated ETEC vaccine called ETVAX (R). In a study involving Zambian children, the use of a proteome microarray showed that post-vaccination antibody responses were stronger against certain ETEC proteins compared to pre-vaccination levels. However, there were also cross-reactive responses observed in the placebo group, indicating the need for further research.
Article
Multidisciplinary Sciences
Zhili Yu, Yaoming Wu, Muyuan Chen, Tong Huo, Wei Zheng, Steven J. Ludtke, Xiaodong Shi, Zhao Wang
Summary: In this study, the authors investigated the secretins GspD(& alpha;) and GspD(& beta;) in Escherichia coli and determined their intermediate structures during the translocation process using Cryo-ET. The obtained structures revealed different membrane interaction patterns and peptidoglycan layer transitions, suggesting two distinct models for the membrane translocation of GspD(& alpha;) and GspD(& beta;). These findings provide valuable insights into the biogenesis of T2SS secretins and their role in bacterial toxin secretion.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Guocheng Xie, Sijia Gao, Jinzhao Ou, Meng Zhu, Man Wu, Xiaoyan Ju, Zhuang Li, Ye Tian, Zhongwei Niu
Summary: By conjugating peptides onto tobacco mosaic virus, the resulting nanoparticles show significantly enhanced antibacterial effects against Gram-negative bacteria. The high osmotic pressure and reactive oxygen species generated by the peptide-TMV cause the death of Escherichia coli, while also downregulating biofilm-related genes to inhibit biofilm formation. This study opens up new possibilities for combating Gram-negative bacteria-related infections.
Review
Microbiology
Claire Maher, Karl A. Hassan
Summary: Gram-negative bacteria are resistant to antibiotics due to their cell envelope's permeability barrier. Understanding the role of different components in the envelope is crucial for developing new broad-spectrum antibiotics.
Article
Medicine, General & Internal
Jorge Calderon Parra, Daniel De Castro-Campos, Patricia Munoz Garcia, Maria Olmedo Samperio, Mercedes Marin Arriaza, Aristides De Alarcon, Encarnacion Gutierrez-Carretero, Maria Carmen Farinas Alvarez, Jose Maria Miro Meda, Miguel Angel Goneaga Sanchez, Raquel Rodriguez Garcia, Guillermo Ojeda Burgos, Zeltia Valcarce-Gonzalez, Antonio Ramos-Martinez
Summary: IE caused by non-HACEK Gram-Negative Bacilli is a rare infection affecting elderly patients with high comorbidity, nosocomial acquisition, and unfavorable outcome. Factors associated with mortality include age, prosthetic valve, and failure to undergo indicated surgery. Patients treated with quinolone combination therapy had lower mortality rates.
EUROPEAN JOURNAL OF INTERNAL MEDICINE
(2021)
Article
Microbiology
Richard Lueking, Wenjing Wei, Norman S. S. Mang, Jessica K. K. Ortwine, Jessica Meisner
Summary: This study provides additional experience in the use of dalbavancin for off-label indications, particularly for patients who are not eligible for outpatient parenteral antimicrobial therapy. The majority of patients were people who inject drugs and are uninsured. The use of dalbavancin at our institution increased during the COVID-19 pandemic to conserve hospital resources and limit healthcare exposure. Institution-specific cost-saving data with the use of dalbavancin are also provided in this study.
MICROBIOLOGY SPECTRUM
(2023)
Article
Agricultural Engineering
Nikolai Khramtsov, Luise McDade, Alexander Amerik, Esther Yu, Kunjan Divatia, Alexander Tikhonov, Michael Minto, Georges Kabongo-Mubalamate, Zdenek Markovic, Marie Ruiz-Martinez, Steven Henck
BIORESOURCE TECHNOLOGY
(2011)
Article
Immunology
Stefan Fernandez, Emily D. Cisney, Alexander P. Tikhonov, Barry Schweitzer, Robert J. Putnak, Monika Simmons, Robert G. Ulrich
CLINICAL AND VACCINE IMMUNOLOGY
(2011)
Article
Multidisciplinary Sciences
Sarah Keasey, Christine Pugh, Alexander Tikhonov, Gengxin Chen, Barry Schweitzer, Aysegul Nalca, Robert G. Ulrich
Article
Biochemical Research Methods
Kara Schmid, Sarah L. Keasey, Phillip Pittman, Ginny L. Emerson, James Meegan, Alexander P. Tikhonov, Gengxin Chen, Barry Schweitzer, Robert G. Ulrich
PROTEOMICS CLINICAL APPLICATIONS
(2008)
Article
Microbiology
David Dodds, Jeffrey L. Bose, Ming-De Deng, Gilles R. Dube, Trudy H. Grossman, Alaina Kaiser, Kashmira Kulkarni, Roger Leger, Sara Mootien-Boyd, Azim Munivar, Julia Oh, Matthew Pestrak, Komal Rajpur, Alexander P. Tikhonov, Traci Turecek, Travis Whitfill
Article
Multidisciplinary Sciences
Changhui Guan, Peter J. Larson, Elizabeth Fleming, Alexander P. Tikhonov, Sara Mootien, Trudy H. Grossman, Caroline Golino, Julia Oh
Summary: Researchers have developed a skin probiotic that can detect and respond to the presence of S. aureus, releasing antimicrobials only when the target organism is present. In vitro testing has demonstrated the activity of this biosensor, and challenges to the deployment of engineered topical skin probiotics are discussed.
Article
Biochemistry & Molecular Biology
Raul Alvarez-Venegas, Monther Sadder, Alexander Tikhonov, Zoya Avramova
MOLECULAR BIOLOGY AND EVOLUTION
(2007)
Article
Biochemistry & Molecular Biology
AP Tikhonov, JL Bennetzen, ZV Avramova
Article
Biochemistry & Molecular Biology
AP Tikhonov, L Lavie, C Tatout, JL Bennetzen, Z Avramova, JM Deragon
CHROMOSOME RESEARCH
(2001)