Article
Biochemistry & Molecular Biology
Amanda Gabrielle da Silva, Luiz Ricardo Goulart, Philipp Loffler, Christian Code, Adriana Freitas Neves
Summary: This study proposed a method using aptamers to capture viral RNA in combination with RT-PCR, which can improve the detection rate of dengue and Zika viruses. Experimental results demonstrate the potential application of this method in rapid molecular detection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Yixin Liu, Binxiao Li, Ya-Jun Wang, Zihui Fan, Yang Du, Bin Li, Yan-Jun Liu, Baohong Liu
Summary: In this study, enzyme-free-mediated cascade amplified nanoprobes were designed for in situ single-molecule imaging of dual-microRNAs in switchable migrating cells. The differential expression and localization of dual-miRNAs were clearly exhibited in multiple cell lines due to the enhanced sensitivity via the cascade signal amplification strategy. Moreover, in situ three-dimensional imaging of dual-miRNAs in transition of cell migration phenotypes was successfully reconstructed in both non-confined and confined microenvironments in vitro, demonstrating differential spatial distribution in a single cell. This holds great promise for exploring the key roles of spatial RNA distribution at the single-molecule level in migrating cells, and for advancing the understanding of molecular mechanisms and physical principles in 3D cell migration in vivo.
ANALYTICAL CHEMISTRY
(2022)
Article
Virology
Manon Broutin, Fleur Costa, Sandy Peltier, Jennifer Maye, Nicolas Versille, Bernard Klonjkowski
Summary: There is a need for highly effective vaccines for veterinary infectious diseases. Canine adenovirus type 2 (CAV2) vectors have shown potential in developing multiple vaccines. In this study, a CAV2 vector encoding the rabies virus glycoprotein was tested with and without an oil-based adjuvant. The adjuvant increased rabies antibody titers and did not affect the anti-CAV2 serological responses. The study also identified host antiviral genes modulated after vaccination, revealing the up-regulation of certain signaling pathways.
Article
Chemistry, Analytical
Cheng Fang, Yuming Li, Song Hu, Hao Wang, Xiaoxia Chen, Xiaoli Zhu
Summary: The developed DNA tree in this study enables amplified analysis of mRNA in living cells, achieving sensitive, accurate, and nondestructive analysis. The probes are transported into cells by exosomes, are LDNAs resistant to degradation, laying the foundation for reliable analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Daniel Plante, Julio Alexander Bran Barrera, Maude Lord, Irene Iugovaz, Neda Nasheri
Summary: A method for extracting viral RNA from raw oysters has been developed, showing high efficiency in removing RT-PCR inhibitors and reliably detecting viral contamination. The method outperformed the ISO 15216-1 method and showed comparable efficiency to Method E, with fewer steps and shorter hands-on time.
Article
Plant Sciences
Yingying Song, Xinlei Guo, Jian Wu, Jianli Liang, Runmao Lin, Zifu Yan, Xiaowu Wang
Summary: This study successfully located gene expression in guard cells of Brassica rapa through optimized in situ RT-PCR protocol, providing a sensitive and effective method for detecting guard cell specific genes.
HORTICULTURAL PLANT JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Hsin-Hung Lin, Kuan-Hung Lin, Kuan-Fu Wu, Yu-Chi Chen
Summary: IbACP, a peptide from sweet potato leaves, affects ROS accumulation and defense gene expression in sweet potato plants. Enhanced POD activity by IbACP treatment suggests a potential mechanism for scavenging H2O2. Genes related to IbACP were classified into various categories, indicating its importance in genetic regulation in sweet potato.
Article
Plant Sciences
Amol D. Kokane, Sunil B. Kokane, Ashish J. Warghane, Mrugendra G. Gubyad, Ashwani Kumar Sharma, M. Krishna Reddy, Dilip Kumar Ghosh
Summary: ICRSV is a devastating pathogen affecting 'Kinnow mandarin' in northwest India, highlighting the need for sensitive and rapid detection methods.
Article
Chemistry, Multidisciplinary
Xander F. van Kooten, Yana Rozevsky, Yulia Marom, Efrat Ben Sadeh, Amit Meller
Summary: Most RNA-based COVID-19 diagnostic methods use enzymatic amplification for high sensitivity but rely on arbitrary thresholding. This study introduces a solid-state nanopore sensing technology for label-free quantification of SARS-CoV-2 RNA in clinical nasal swab samples. The method allows for ratiometric quantification of RNA abundance through single-molecule counting and length-based nanopore identification of dsDNA. The results show that this approach can provide quantitative positive outcomes in clinical samples classified as negative by RT-qPCR.
Article
Fisheries
Gary F. Kett, Sarah C. Culloty, Marcel A. K. Jansen, Sharon A. Lynch
Summary: A novel generic PCR and DIG-labeled in situ hybridization method were developed to increase diagnostic capabilities for Vibrio bacteria, allowing for blanket screening of hosts and environmental samples and visual localization of bacteria. These techniques can be used in conjunction with specific methods for certain Vibrio species, enhancing the diagnostic arsenal against this globally significant genus of bacteria.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
Chia-Yu Hsieh, Tirawat Rairat, Chi-Chung Chou
Summary: Researchers in southern Taiwan observed higher mortality rates in American bullfrogs from July 2007 to January 2012, with skin ulcerations on the dorsal surface of infected bullfrogs being a common symptom. Ultrastructural examination revealed the presence of Ranavirus particles in splenic phagocytes. Ranavirus was determined to be the main cause of economic losses in Taiwanese bullfrog aquaculture, with isolates closely related to strains from Korea and Japan.
Article
Entomology
Kirsten Warcup, Alex S. Torson, George D. Yocum
Summary: "Research on Sex Determination Methods of Colorado Potato Beetles" used publicly available RNA-seq data to select 5 candidate genes for sex-specific markers in adult Colorado potato beetles. The study confirmed that these 5 marker candidates exhibit a sex-specific expression pattern and can be used as PCR markers for sex determination. This method allows for simple sex detection using cDNA as the template and assigning sex to RNA-seq samples post hoc.
JOURNAL OF INSECT SCIENCE
(2023)
Article
Pathology
Hoi Yi Leung, Martin Ho Yin Yeung, Wai Tung Leung, King Hin Wong, Wai Yan Tang, William Chi Shing Cho, Heong Ting Wong, Hin Fung Tsang, Yin Kwan Evelyn Wong, Xiao Meng Pei, Hennie Yuk Lin Cheng, Amanda Kit Ching Chan, Sze Chuen Cesar Wong
Summary: ISH has evolved from simply identifying the chromosomal location of human genes to being extensively used for gene expression studies and clinical diagnosis, especially in identifying biomarkers for diseases such as breast and ovarian cancer. Advances in ISH technologies and training have the potential to improve diagnosis in Anatomical Pathology.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2022)
Article
Biochemistry & Molecular Biology
Mei Yang, Ruihua Ji, Zhengqing Zhao, Wenwen Wang, Ye Lu, Zhenghua Xiang, Hongbin Yuan
Summary: This study established a new method called In Situ Hybridization Chain Reaction (isHCR) for in situ detection of apoptotic DNA fragments. Compared to the current TUNEL method, isHCR can specifically detect DNA fragmentation in the cytoplasm of early-stage apoptotic cells, with low cost and the ability to handle large numbers of specimens. This suggests that isHCR has the potential to play a role in the in situ detection of DNA fragmentation in apoptotic cells.
Article
Microbiology
Xue-Lian Luo, Xiu-Dan Zhang, Bei-Jie Li, Tian Qin, Zhi-Jie Cao, Qian-Jin Fan, Jing Yang, Dong Jin, Shan Lu, Ya-Yun Zheng, Xue-Fang Xu, Ji Pu, Jianguo Xu
Summary: Lassa virus is an important human pathogen causing hemorrhagic fever in West Africa, with an increasing risk of imported cases to other countries due to increased traveling. The high nucleotide diversity of the virus strains complicates the development of diagnostic assays. The GPC RT-PCR/2007 assay and the Mabsky kit showed good sensitivity and specificity for detecting most LASV strains.
MICROBIOLOGY SPECTRUM
(2023)
Article
Cell Biology
Marco Gambarotti, Angelo P. Dei Tos, Daniel Vanel, Piero Picci, Dino Gibertoni, Michael J. Klein, Alberto Righi
Correction
Orthopedics
Shinji Tsukamoto, Andreas F. Mavrogenis, Giulio Leone, Alberto Righi, Manabu Akahane, Piergiuseppe Tanzi, Akira Kido, Kanya Honoki, Yasuhito Tanaka, Davide Maria Donati, Costantino Errani
INTERNATIONAL ORTHOPAEDICS
(2019)
Article
Orthopedics
Shinji Tsukamoto, Andreas F. Mavrogenis, Giulio Leone, Alberto Righi, Manabu Akahane, Piergiuseppe Tanzi, Akira Kido, Kanya Honoki, Yasuhito Tanaka, Davide Maria Donati, Costantino Errani
INTERNATIONAL ORTHOPAEDICS
(2019)
Article
Endocrinology & Metabolism
S. Asioli, A. Righi, M. Iommi, C. Baldovini, F. Ambrosi, F. Guaraldi, M. Zoli, D. Mazzatenta, M. Faustini-Fustini, P. Rucci, C. Giannini, M. PFoschini
EUROPEAN JOURNAL OF ENDOCRINOLOGY
(2019)
Article
Oncology
Shinji Tsukamoto, Andreas F. Mavrogenis, Piergiuseppe Tanzi, Giulio Leone, Alberto Righi, Manabu Akahane, Akira Kido, Kanya Honoki, Yasuhito Tanaka, Davide Maria Donati, Costantino Errani
JOURNAL OF SURGICAL ONCOLOGY
(2019)
Article
Oncology
Marilena Cesari, Alberto Righi, Marco Colangeli, Marco Gambarotti, Paolo Spinnato, Andrea Ferraro, Alessandra Longhi, Massimo Eraldo Abate, Emanuela Palmerini, Anna Paioli, Cristina Ferrari, Davide Maria Donati, Piero Picci, Stefano Ferrari
PEDIATRIC BLOOD & CANCER
(2019)
Article
Oncology
Emanuela Palmerini, Roberta Sanfilippo, Giovanni Grignani, Angela Buonadonna, Antonella Romanini, Giuseppe Badalamenti, Virginia Ferraresi, Bruno Vincenzi, Alessandro Comandone, Antonio Pizzolorusso, Antonella Brunello, Fabio Gelsomino, Tommaso De Pas, Toni Ibrahim, Federica Grosso, Francesca Zanelli, Maria Abbondanza Pantaleo, Laura Milesi, Libero Ciuffreda, Vittorio Ferrari, Emanuela Marchesi, Irene Quattrini, Alberto Righi, Elisabetta Setola, Elisa Carretta, Piero Picci, Stefano Ferrari
Summary: This study examined the real-world efficacy and safety of trabectedin in treating advanced STS patients in Italy. Results showed that trabectedin is most commonly used as a second-line treatment for patients with high-grade, metastatic leiomyosarcoma and liposarcoma, with manageable safety profiles. The study suggests that trabectedin is an active treatment for advanced STS, especially in patients with L-sarcoma histologies.
Review
Oncology
Giuseppe Bianchi, Debora Lana, Marco Gambarotti, Cristina Ferrari, Marta Sbaraglia, Elena Pedrini, Laura Pazzaglia, Luca Sangiorgi, Isabella Bartolotti, Angelo Paolo Dei Tos, Katia Scotlandi, Alberto Righi
Summary: In contrast to SFT of soft tissues, aggressive behavior of primary SFT of the bone appears to be independent from mitotic count or any other clinicopathological and molecular features.
Article
Oncology
Shinji Tsukamoto, Alberto Righi, Andreas F. Mavrogenis, Manabu Akahane, Kanya Honoki, Yasuhito Tanaka, Davide Maria Donati, Costantino Errani
Summary: In cases of giant cell tumor of bone (GCTB), an intermediate malignant bone tumor, approximately 4% experience malignant transformation. Local recurrence is an independent prognostic factor for unfavorable malignant transformation, with late local recurrence of GCTB being associated with a higher risk of malignant transformation.
Article
Oncology
Francesca Ambrosi, Alberto Righi, Stefania Benini, Giovanna Magagnoli, Ilaria Chiaramonte, Marco Manfrini, Alessandro Gasbarrini, Tommaso Frisoni, Marco Gambarotti
Summary: Giant cell tumor of the bone is a rare, locally aggressive tumor that typically occurs in patients between 20 and 45 years old. Immunohistochemical and molecular analysis can effectively diagnose H3F3A gene mutations in this tumor.
Review
Oncology
Rokuro Morii, Shinji Tsukamoto, Alberto Righi, Kanya Honoki, Yuu Tanaka, Akira Kido, Hiromasa Fujii, Andreas F. Mavrogenis, Yasuhito Tanaka, Costantino Errani
Summary: The effect of adjuvant chemotherapy on primary localized malignant GCTB remains unclear, but it can improve survival rates for patients with secondary localized malignant GCTB.
Article
Oncology
Sofia Avnet, Silvia Lemma, Margherita Cortini, Gemma Di Pompo, Francesca Perut, Maria Veronica Lipreri, Laura Roncuzzi, Marta Columbaro, Costantino Errani, Alessandra Longhi, Nicoletta Zini, Dominique Heymann, Massimo Dominici, Giulia Grisendi, Giulia Golinelli, Lorena Consolino, Dario Livio Longo, Cristina Nanni, Alberto Righi, Nicola Baldini
Summary: The study validated the correlation between tumor glycolysis/acidosis and metastasis, and investigated the role of acidity-induced inflammation in the development of metastasis in osteosarcoma.
Review
Oncology
Shinji Tsukamoto, Andreas F. Mavrogenis, Lucia Angelelli, Alberto Righi, Giuseppe Filardo, Akira Kido, Kanya Honoki, Yuu Tanaka, Yasuhito Tanaka, Costantino Errani
Summary: The 5-year disease-free survival rate for patients with localized extraskeletal osteosarcoma (ESOS) who underwent surgery combined with (neo)adjuvant chemotherapy was slightly higher than those who underwent surgery alone. Therefore, routine adjuvant chemotherapy for localized ESOS should be avoided.
Article
Biochemistry & Molecular Biology
Stefania Benini, Gabriella Gamberi, Stefania Cocchi, Giovanna Magagnoli, Angela Rosa Fortunato, Enrica Sciulli, Alberto Righi, Marco Gambarotti
Summary: This study evaluated the diagnostic utility of molecular analysis compared with histological assessment in sarcoma. It demonstrated the accuracy of c/qRT-PCR and FISH for predicting sarcoma subtypes, facilitating the differential diagnosis of these tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Silvia Terzi, Federica Trentin, Cristiana Griffoni, Elisa Carretta, Stefano Bandiera, Cristina Ferrari, Fabio Vita, Alberto Righi, Margherita Maioli, Dario De Biase, Annalisa Monetta, Giovanni Barbanti Brodano, Gisberto Evangelisti, Marco Girolami, Valerio Pipola, Marco Gambarotti, Alessandro Gasbarrini
Summary: This retrospective study aimed to evaluate the overall survival of surgically treated patients with lung cancer spinal metastases and identify factors associated with better survival. The results showed that surgical treatment can improve the quality of life and control pain, thereby enhancing autonomy.
Article
Pathology
Juliana Mota Siqueira, Yoshitsugu Mitani, Camilla Oliveira Hoff, Flavia Bonini, Luana Guimaraes de Sousa, Mario L. Marques-Piubelli, Anurag Purushothaman, Mutsumi Mitani, Hui Dai, Shiaw-Yih Lin, Michael T. Spiotto, Ehab Y. Hanna, Daniel J. McGrail, Adel K. El-Naggar, Renata Ferrarotto
Summary: B7-H4 expression pattern varies among different types of salivary gland carcinomas, and high B7-H4 expression is associated with poor prognosis in adenoid cystic carcinoma.
Article
Pathology
Basile Tessier-Cloutier, Felix K. F. Kommoss, David L. Kolin, Kristyna Nemejcova, Dupreez Smith, Jennifer Pors, Colin J. R. Stewart, W. Glenn Mccluggage, William D. Foulkes, Andreas von Deimling, Martin Kobel, Cheng-Han Lee
Summary: This study provides a detailed analysis of the clinical, pathological, immunohistochemical, and molecular features of DDOC/UDOC. The majority of patients presented with extraovarian disease and had rapid disease progression resulting in high mortality rate.
Review
Pathology
Sophia J. Wagner, Christian Matek, Sayedali Shetab Boushehri, Melanie Boxberg, Lorenz Lamm, Ario Sada, Dominik J. E. Winter, Carsten Marr, Tingying Peng
Summary: Computational pathology research driven by deep learning faces challenges in reproducibility and reusability. Codebase with good documentation and robustness and generalizability of models are crucial. The reuse of computational pathology algorithms is limited, and their application in clinical settings is even rarer. This study evaluates 160 peer-reviewed articles, providing criteria for data and code availability and statistical analysis of results.
Article
Pathology
Andres M. Acosta, Lynette M. Sholl, Fiona Maclean, Chia-Sui Kao, Thomas M. Ulbright
Summary: This study assessed the clinicopathologic and genomic features of 14 cases of testicular sex cord-stromal tumors. The results showed that CTNNB1 mutations are rare in these tumors, and most of them have genomic alterations similar to testicular sex cord-stromal tumors with pure or predominant spindle cell components.
Article
Pathology
Toru Odate, Kaishi Satomi, Takashi Kubo, Yuko Matsushita, Toshihide Ueno, Akira Kurose, Kohei Shomori, Tokiko Nakai, Reiko Watanabe, Keiko Segawa, Shusa Ohshika, Naritomo Miyake, Sayaka Kudo, Tatsunori Shimoi, Eisuke Kobayashi, Motokiyo Komiyama, Seiichi Yoshimoto, Fumihiko Nakatani, Akira Kawai, Yasushi Yatabe, Shinji Kohsaka, Koichi Ichimura, Hitoshi Ichikawa, Akihiko Yoshida
Summary: Inflammatory rhabdomyoblastic tumors (IRMTs) are newly recognized skeletal muscle tumors with uncertain malignant potential. This study investigated 13 IRMTs using clinicopathologic, genetic, and epigenetic methods. The results showed specific histologic features and genetic mutations in these tumors, and most of them exhibited benign behavior.
Article
Pathology
Dale L. Davis, Adam C. Lechner, David B. Chapel, Jonathan C. Slack, Chrystalle Katte Carreon, Bradley J. Quade, Carlos Parra-Herran
Summary: The Amsterdam Consensus Statement introduced the term maternal vascular malperfusion (MVM) to classify a group of findings related to impaired maternal-placental circulation. The study found that features such as low placental weight, accelerated villous maturation, decidual arteriopathy, and infarcts are associated with adverse obstetrical outcomes, while the role of other features like distal villous hypoplasia, excess multinucleated trophoblast, and retroplacental hemorrhage needs further research.
Review
Pathology
Alain C. Borczuk
Summary: COVID-19 is an acute respiratory illness that can progress to acute respiratory distress syndrome. While most patients recover completely, some may experience persistent respiratory dysfunction, known as long COVID. The pathogenesis involves immune and cellular disturbances.
Article
Pathology
Annikka Weissferdt, Cheuk H. Leung, Heather Lin, Boris Sepesi, William N. William, Stephen G. Swisher, Tina Cascone, J. Jack Lee, Abujiang Pataer
Summary: Neoadjuvant treatment of non-small cell lung cancer challenges traditional processing of pathology specimens, and accurate evaluation of residual tumor is crucial for assessing treatment efficacy.