Article
Fisheries
Hagilaa Ganesan, Kok Lian Ho, Abdul Razak Mariatulqabtiah, Chean Yeah Yong, Chuan Loo Wong, Zee Hong Goh, Jia Sen Tan, Wen Siang Tan
Summary: This study identified the minimum contiguous sequence of the capsid protein (CP) of MrNv virus required for virus-like particle (VLP) formation. Truncation and deletion experiments revealed that the N-terminal arm (NTA) and residues involved in dimeric interaction in the shell (S) domain play crucial roles in VLP assembly. These findings provide mechanistic insights into the assembly of MrNv VLP and expand its potential applications in vaccine development and nucleic acid delivery.
Article
Biochemistry & Molecular Biology
Bethilda Anne Selvaraj, Abdul Razak Mariatulqabtiah, Kok Lian Ho, Chyan Leong Ng, Chean Yeah Yong, Wen Siang Tan
Summary: This study aims to enhance the stability of MrNV CP by modulating protease degradation activity. The addition of a specific protease inhibitor E-64 significantly increased the yield of MrNV CP, providing an economical method to improve scalability for downstream applications such as drug delivery and vaccine development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Gulshan Kumar, A. K. Singh, Deepak Agarwal
Summary: Macrobrachium rosenbergii, a valuable farmed freshwater species, is globally affected by white tail disease caused by M. rosenbergii nodavirus (MrNV). MrNV is an important drug target for pathogenesis, encoding RNA-dependent RNA polymerase (RdRp) for genome replication. The predicted three-dimensional structure of MnRdRp shows similarity with other flaviviridae members. The study identified metal binding residues and potential inhibitors for MnRdRp.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Fisheries
Yupei Tong, Jia Yang, Li Wang, Xiaoping Chi, Chan Zhu, Rong Yin, Le Zhang, Yaru Li, Chunyan Zhao, Rui Jia
Summary: Feeding Macrobrachium nipponense with Anabaena sp. PCC 7120 expressing VP28 can reduce the probability of WSSV infection and improve the survival rate and disease resistance of the shrimp.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Jitao Xia, Chong Wang, Liang Yao, Wei Wang, Wenxiu Zhao, Tianchang Jia, Xingtong Yu, Guoliang Yang, Qingli Zhang
Summary: This study detected the presence of Covert mortality nodavirus (CMNV) in farmed giant freshwater prawn (Macrobrachium rosenbergii) in Jiangsu, China using TaqMan RT-qPCR. The study identified the intestine, gill, hepatopancreas, and ovary as target organs of CMNV infection in giant freshwater prawn. In addition, the study observed CMNV-like particles in the hepatopancreas and gill tissues. These findings further expand our knowledge on CMNV and provide new insights for investigating the pathogenic factors of giant freshwater prawn disease.
Article
Fisheries
Gwang Il Jang, Bo Seong Kim, Su Mi Kim, Yun Kyeong Oh, Jae Ok Kim, Jee Youn Hwang, Soon Jeong Lee, Sung Youl Hong, Mun Gyeong Kwon
Summary: White tail disease is caused by Macrobrachium rosenbergii nodavirus (MrNV) and an extra-small virus (XSV). This study detected MrNV in both adult and post-larval prawns, while XSV was not detected. Although no gross signs of the disease were observed, histopathological changes were found in the muscle of adult and post-larval prawns.
Article
Virology
Vincenzo A. Costa, Jemma L. Geoghegan, Edward C. Holmes, Erin Harvey
Summary: This study reports the occurrence of the Macrobrachium rosenbergii nodavirus (MrNV) outside of the Queensland region in Australia and provides evidence for genetic reassortment between endemic and introduced MrNV. In addition, it suggests that Macrobrachium australiense may be an important reservoir host for MrNV.
Article
Fisheries
Wattana Weerachatyanukul, Chettupon Pooljun, Ikuo Hirono, Charoonroj Chotwiwatthanakun, Pitchanee Jariyapong
Summary: It has been confirmed that the baculovirus-insect cell line is suitable for the replication, propagation, and secretion of shrimp virus in vitro. The study aimed to produce a clone of Macrobrachium rosenbergii nodavirus (MrNV) within S2 cells to improve viral production compared to the previous model. The results showed that S2 cells were permissive for MrNV and the produced virus was useful for testing MrNV infection and investigating viral pathogenesis.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Review
Fisheries
Ken Fong Chen, Wen Siang Tan, Lin Kooi Ong, Syafiq Asnawi Zainal Abidin, Iekhsan Othman, Beng Ti Tey, Ronald Fook Seng Lee
Summary: The review discusses key aspects of Macrobrachium rosenbergii nodavirus, including its structure, transmission and infection mechanisms, and common strategies for detection and prevention, highlighting the importance of understanding the virus for effective outbreak control.
REVIEWS IN AQUACULTURE
(2021)
Article
Zoology
Feng Lin, Jinyu Shen, Yuelin Liu, Aixia Huang, Haiqi Zhang, Fan Chen, Dongren Zhou, Yang Zhou, Guijie Hao
Summary: Nucleic acid sequence-based amplification (NASBA) combined with an immunoassay-based lateral flow dipstick (LFD) is a rapid, accurate, sensitive, and specific detection method for MrNV from China. The assay showed a sensitivity 104 times higher than the present RT-PCR approach, and it was specific for MrNV without creating products in shrimps with other virus infections. Early detection of this infectious disease is important for implementing efficient therapeutic strategies, preventing its spread, and limiting loss of aquatic breeds.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2023)
Article
Fisheries
Ken Fong Chen, Sathiya Maran, Wen Siang Tan, Lin Kooi Ong, Syafiq Asnawi Zainal Abidin, Iekhsan Othman, Beng Ti Tey, Ronald Fook Seng Lee
Summary: This meta-analysis assesses the effectiveness of different prophylactic agents in promoting the survival of giant freshwater prawns infected with Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV). The study found that DNA/RNA prophylaxis, viral protein-based prophylaxis, and supplements all provided protection against MrNV infection. A shorter incubation period and prophylactic agents derived from MrNV origin were found to be more effective. However, a high degree of heterogeneity was detected between studies, indicating the need for future large-scale studies.
Article
Fisheries
Snehal Jamalpure, S. Vimal, A. Nafeez Ahmed, A. S. Sahul Hameed, Kishore M. Paknikar, Jyutika M. Rajwade
Summary: Macrobrachium rosenbergii nodavirus (MrNV) causes white tail disease (WTD) in larval, post-larval, and juvenile stages of the giant freshwater prawn M. rosenbergii. An antibody-based lateral flow assay (LFA) was developed and optimized for early detection of MrNV in the post-larval stage with high specificity, reproducibility, and sensitivity. The LFA showed good agreement with the 'gold standard' qRT-PCR test, demonstrating high sensitivity (100%) and specificity (90%).
Article
Fisheries
Chutima Srisuk, Chanitcha Choolert, William G. Bendena, Siwaporn Longyant, Paisarn Sithigorngul, Parin Chaivisuthangkura
Summary: This study isolated the cDNA of hemocyanin isoform 2 of the prawn Macrobrachium rosenbergii (MrHc2) and characterized its mRNA expression. The study found that MrHc2 contains three domains and was significantly expressed when challenged with MrNV, suggesting its important role in the innate immune response of M. rosenbergii to MrNV.
JOURNAL OF AQUATIC ANIMAL HEALTH
(2022)
Article
Biology
S. Thanigaivel, John Thomas, A. S. Vickram, K. Anbarasu, Rohini Karunakaran, Jeyanthi Palanivelu, P. S. Srikumar
Summary: The study demonstrates the biological synthesis of AgNPs by Ch. antennia against V. harveyi in M. rosenbergii, showing effective antibacterial activity. Oral administration of encapsulated AgNPs significantly improves the survival rate in vivo.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Alejandro Hernandez-Soto, Randall Chacon-Cerdas
Summary: RNAi technology is a versatile, effective, and eco-friendly alternative for crop protection, with applications for controlling viruses, bacteria, fungi, insects, and nematodes through HIGS and SIGS approaches. Encapsulation of RNAi in materials such as liposomes is a key focus for ensuring stability and controlled release, with challenges including stability, biodegradability, and biocompatibility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Kwanta Thansa, Thanapong Kruangkum, Arnon Pudgerd, Lamai Chaichandee, Piti Amparyup, Rungkarn Suebsing, Charoonroj Chotwiwatthanakun, Rapeepun Vanichviriyakit, Kallaya Sritunyalucksana
Summary: The study achieved in vitro culture of hematopoietic tissue cells of the giant freshwater prawn, and confirmed their potential for studying pathogens and hemocyte development. The presence of hematopoietic stem cell markers in the cultured cells indicated their ability to differentiate into mature blood cells.
Article
Fisheries
Arnon Pudgerd, Thanapong Kruangkum, Kallaya Sritunyalucksana, Rapeepun Vanichviriyakit, Supapong Imsonpang, Charoonroj Chotwiwatthanakun
Summary: Hemocyte reduction in fatally VPAHPND-infected prawns is caused by an acute self-destructive immune response by hematopoietic cells.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Channapha Sakseepipad, Reiko Nozaki, Keisuke Yoshii, Yutaka Fukuda, Kaori Mizuno, Hidemasa Kawakami, Ikuo Hirono, Hidehiro Kondo
Summary: This study developed two primer sets to amplify SNP regions of RSIV-type megalocytiviruses, identifying 53 polymorphic sites and clustering 25 isolates into eight haplotypes through phylogenetic analysis. These findings suggest that SNPs in the RSIV genome are a powerful tool for detection and identification of RSIV-type megalocytiviruses.
JOURNAL OF FISH DISEASES
(2021)
Article
Genetics & Heredity
Masaya Sato, Satoshi Kawato, Hikaru Oyama, Gen Kaneko, Eric J. Post, Rei Suo, Noriyuki Takai, Haruo Sugita, Hidehiro Kondo, Ikuo Hirono, Shiro Itoi
Summary: The complete mitochondrial genome of the Atlantic Gnomefish was determined, revealing distinct phylogenetic relationships with Japanese and African populations based on the analysis of mitochondrial DNA and cytochrome c oxidase subunit I gene.
MITOCHONDRIAL DNA PART B-RESOURCES
(2021)
Article
Microbiology
Kei Kagaya, Rika Kogure, Masahiko Okai, Satoshi Kawato, Hidehiro Kondo, Ikuo Hirono, Masami Ishida
Summary: This article presents the draft genome sequences of two lipid-degrading bacteria strains, Moritella sp. F1 and F3, isolated from intermediate water in the Sagami Trough, Japan. The genome sizes of these strains are 4,983,334 bp and 4,967,310 bp respectively.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Microbiology
Satoshi Kawato, Reiko Nozaki, Ikuo Hirono, Hidehiro Kondo
Summary: The genome sequence of lymphocystis disease virus 2 LCDV-JP_Oita_2018, isolated from a diseased Japanese flounder in Japan, is presented here. The circular contig of LCDV-JP_Oita_2018 genome is 186,627 bp long, with 140 predicted protein-coding genes and a GC content of 27%.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Fisheries
Wattana Weerachatyanukul, Chettupon Pooljun, Ikuo Hirono, Hidehiro Kondo, Charoonroj Chotwiwatthanakun, Pitchanee Jariyapong
Summary: This study evaluated the immune-stimulating effect of infectious hypodermal and hematopoietic necrosis virus like particle (IHHNV-VLP) in Fenneropenaeus merguiensis and identified the antioxidative gene, peroxiredoxin (FmPrx), as a key gene that is up-regulated after VLP injection. The up-regulation of FmPrx enhances the level of immune-related genes and peroxidase activity, thereby improving the shrimp's immune system.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Prawporn Thaijongrak, Charoonroj Chotwiwatthanakun, Phaivit Laphyai, Anuphap Prachumwat, Thanapong Kruangkum, Prasert Sobhon, Rapeepun Vanichviriyakit
Summary: In this study, researchers identified and characterized genes PmOvtra2, Pmfru-1, and Pmfru-2, which are involved in sex determination pathways in the species P. monodon. They investigated the expression patterns and functions of these genes and found that they are expressed in the undifferentiated gonads during early developmental stages, with significantly higher expression in the ovaries compared to the testes. This suggests that these genes play important roles in female sex determination in P. monodon.
Article
Genetics & Heredity
Wafaa Ragab, Satoshi Kawato, Reiko Nozaki, Hidehiro Kondo, Ikuo Hirono
Summary: This study presents a comparative genome analysis of Vibrio penaeicida, revealing its genetic features, evolution, and pathogenicity. The results indicate that the strains belong to the same species and possess typical virulence factors and other related genes. These findings are important for understanding the pathogenesis of Vibrio penaeicida.
MICROBIAL GENOMICS
(2022)
Article
Virology
Wattana Weerachatyanukul, Pauline Kiatmetha, Ponlawoot Raksat, Supawich Boonkua, Orawan Thongsum, Pitchanee Jariyapong, Charoonroj Chotwiwatthanakun, Puey Ounjai, Zoltan Metlagel
Summary: This study aimed to encapsulate large-sized double-stranded DNA (dsDNA) into a small-sized virus-like particle and compare the changes in capsid structure between dsDNA-filled and empty particles. The results showed that the dsDNA-filled particles were slightly bigger and had a thicker capsid structure compared to the empty particles. The shape of the capsid exterior also changed, particularly at the five-fold axes. The ability to encapsulate dsDNA opens up opportunities for gene delivery and targeting susceptible cells to combat viral infections in shrimp.
Article
Virology
Satoshi Kawato, Shoya Fujishima, Reiko Nozaki, Hidehiro Kondo, Tadashi Isshiki, Ikuo Hirono
Summary: This study reports the causative agent of milky hemolymph disease in snow crab, known as Chionoecetes opilio bacilliform virus (CoBV), and reveals its complete genome sequence, confirming its classification as a member of the Nimaviridae family.
ARCHIVES OF VIROLOGY
(2023)
Article
Fisheries
Mahmoud Mostafa Mahmoud, Mohamed Abdelsalam, Satoshi Kawato, Shogo Harakawa, Hidemasa Kawakami, Ikuo Hirono, Hidehiro Kondo
Summary: This study compared the whole genome sequences of three different serotypes of Lactococcus garvieae isolated from diseased cultured striped jack in Ehime prefecture, Japan. The serotypes showed different virulence in a challenge test. Two serotypes were identified as L. garvieae and one as L. formosensis. The genome sizes ranged from 1.9 to 2.0 Mb, GC content ranged from 38.2% to 38.9%, and the number of predicted protein-coding sequences ranged from 1922 to 1959. Only one serotype harbored two plasmids.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Jayasundara Mudiyanselage Sajani Nisansala Rathnapala, Wafaa Ragab, Satoshi Kawato, Miho Furukawa, Reiko Nozaki, Hidehiro Kondo, Ikuo Hirono
Summary: By sequencing the genomes of three strains of Vibrio nigripulchritudo, it was found that they possess various virulence factors and mobile genetic elements, as well as multidrug resistance. Comparative genomic analysis showed that all three strains belong to the same clade.
JOURNAL OF FISH DISEASES
(2023)
Article
Biotechnology & Applied Microbiology
Keiichiro Koiwai, Hidehiro Kondo, Ikuo Hirono
Summary: In this study, Drop-seq technology was used to investigate the impact of virus infection on hemocyte populations in shrimp. The findings revealed that virus infection reduced specific cell populations in circulating hemolymph and inhibited the expression of antimicrobial peptides. Unknown genes with antimicrobial activity were also identified. Additionally, the study discussed the effects of methanol fixation on Drop-seq data.
MARINE BIOTECHNOLOGY
(2023)
Article
Fisheries
Chalermkwan Eurlaphan, Reiko Nozaki, Motohiko Sano, Keiichiro Koiwai, Ikuo Hirono, Hidehiro Kondo
Summary: This study investigated the kinetics of red sea bream iridovirus and host gene expression during infection in rock bream (Oplegnathus fasciatus). The results showed that viral gene transcripts were detected at 3 and 5 days post-injection, and certain host genes were differentially expressed during the infection process. The downregulation of certain inflammation-related genes may contribute to the increased susceptibility of this fish to the virus.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Hunter S. Bailey, Ashley N. Fincannon, Lee A. Fuiman
Summary: This study investigated the transfer of fatty acids from broodstock diets to eggs and the time needed for the eggs to equilibrate to the diet in Southern flounder. The findings indicate that different diet treatments significantly affect the fatty acid composition of eggs, and it takes 8-16 weeks for the eggs to adapt to changes in the adult diet. These results are important for improving broodstock diets and feeding protocols in stock-enhancement programs.
Article
Fisheries
Yu Liu, Mingtao Lei, Hector Victor, Yan Wang
Summary: This study confirms the feasibility of replacing raw fish diet with a formulated diet in commercial farming of large yellow croaker, which can increase fish survival and yield, and reduce feed cost and body lipid deposition.
Article
Fisheries
Xiaoyan Zhang, Lize San, Yucong Yang, Yuehong Tao, Jiangong Ren, Yufeng Liu, Zhongwei He, Jiashuo Di, Ziteng Pei, Guixing Wang, Jilun Hou
Summary: Gynogenesis is an effective technique for establishing homogeneous lines and confirming potential chromosomal mechanisms of sex determination in fish. This study successfully induced gynogenetic families in Thamnaconus modestus and established a complete method for gynogenesis induction.
Article
Fisheries
Julieta C. Martinelli, Megan Considine, Helen R. Casendino, Carolyn M. Tarpey, Isadora Jimenez-Hidalgo, Jacqueline L. Padilla-Gamino, Teri L. King, Lorenz Hauser, Steven Rumrill, Chelsea L. Wood
Summary: Shell-boring polychaetes have caused significant damage to mariculture operations worldwide, particularly in the US Pacific region. This study provides a comprehensive dataset on the prevalence, species distribution, and environmental associations of these parasites, highlighting the impact of season, culturing methods, and environmental factors on infestation.
Article
Fisheries
Juan Gao, Xueshan Li, Kangle Lu, Kai Song, Jian Zhang, Ling Wang, Chunxiao Zhang
Summary: This study investigated the effects of dietary protein levels on growth performance, antioxidant capacity, digestive enzyme activity, and metamorphosis rate of bullfrog tadpoles. The optimal dietary protein level for bullfrog tadpoles was estimated to be 42.49%-46.50% of the diet.
Article
Fisheries
Qiang Li, Lijun Duan, Dongsheng Jin, Yuxin Chen, Yirong Lou, Qianjin Zhou, Zhongjie Xu, Fangjie Chen, Hongxian Chen, Guizong Xu, Maocang Yan, Guanjun Yang, Jianfei Lu, Yanjun Zhang, Jiong Chen
Summary: This study developed a centrifugal microfluidic chip with on-chip RPA to detect five pathogenic microorganisms. The chip enabled the parallel analysis of six genetic markers from a single sample and allowed for the highly automated detection of multiple samples. Compared with PCR and DNA sequencing, the on-chip RPA assay showed high sensitivity and specificity for detecting multiple pathogens in shrimp aquaculture.
Article
Fisheries
Xingchen Huo, Pengxu Wang, Fengxia Zhao, Qian Liu, Qingqing Tian, Lingjie Tang, Maolin Lv, Zhaohui Wei, Chunrong Yang, Jianguo Su
Summary: Bacterial diseases in aquaculture ponds have negative effects on sustainable development. The use of composite I20 biotherapy has been shown to effectively treat bacterial diseases in bullfrog ponds, providing a new strategy for controlling bacterial diseases in aquaculture.
Article
Fisheries
Peng Yin, Takaya Saito, Per Gunnar Fjelldal, Bjorn Tharandur Bjornsson, Sofie Charlotte Remo, Sandeep Sharma, Rolf Erik Olsen, Kristin Hamre
Summary: This study aims to investigate the effect of seasonal changes in temperature and photoperiod on the redox metabolism of Atlantic salmon at the molecular level. The research findings suggest that the changing photoperiod and temperature influenced the redox metabolism of Atlantic salmon, highlighting the importance of oxidative status in the ecological implications.
Article
Fisheries
Nicholas Oppong Mensah, Jeffery Kofi Asare, Emmanuel Tetteh-Doku Mensah, Ernest Christlieb Amrago, Frank Osei Tutu, Anthony Donkor
Summary: This study investigates aquaculture farmers' preference for climate-smart aquaculture insurance products, the challenges they face, and their preferred insurance coverage. The results show that farmers prefer Climate-Induced Aquaculture Stock Mortality Insurance and the most significant constraint is the delay in claim settlement.
Article
Fisheries
Qiu-Ping Chai, Pei Wu, Wei-Dan Jiang, Yang Liu, Hong-Mei Ren, Xiao-Wan Jin, Lin Feng, Xiao-Qiu Zhou
Summary: The study found that appropriate levels of potassium diformate (KDF) can enhance the immune defense and mediate the inflammatory process in fish, possibly through the regulation of T cell differentiation via JAK-STAT and NF-kappa B signaling pathways.
Article
Fisheries
Tian Zhu, Haomin Jia, Haopeng Zhang, Yujing Xiao, Cui Han, Jiaxin Yang
Summary: Chicken manure has significant effects on the cultivation of Chlorella and rotifers. The optimal amount of chicken manure extract for continuous cultivation is 10 mLL-1. Chicken manure can promote the growth of Chlorella and improve the fatty acid composition, indirectly enhancing the growth condition and fatty acid content of rotifers.
Article
Fisheries
Zheng Luo, Yang Yu, Zhenning Bao, Fuhua Li
Summary: This study analyzed the heritability and genetic correlation of two growth traits in Pacific white shrimp and evaluated the genomic prediction using different genomic selection models. The results showed that the NeuralNet model had the highest prediction accuracy and better prospects for predicting shrimp growth traits.
Article
Fisheries
Alberto Ruiz, Ignasi Sanahuja, Karl B. Andree, Dolors Furones, Paul G. Holhorea, Josep A. Calduch-Giner, Jose J. Pastor, Marc Vinas, Jaume Perez-Sanchez, Sofia Morais, Enric Gisbert
Summary: Supplementing diets with a combination of spice oleoresins can reduce lipid accumulation and inflammation in farmed fish. The combination of spice oleoresins also has an immunomodulatory effect on the fish's intestinal immune system, potentially offering anti-inflammatory benefits.
Article
Fisheries
Beibei Zhao, Renhui Mei, Le Li, Di Hu, Lei Li
Summary: This study reveals that JfCXCL8_L1a and JfCXCL8_L1b have different immune pathways, and JfCXCL8_L1b plays a significant role in enhancing the adaptive immunity of T cell-dependent antigen.
Article
Fisheries
Yuhang Liu, Danying Cao, Nan Wu, Xuyang Zhao, Qingsong Zhu, Lian Su, Fatima Altaf, Qianqian Zhang, Haokun Liu, Yongming Li, Bruno Hamish Unger, Yingyin Cheng, Wanting Zhang, Aihua Li, Yaping Wang, Xiao-Qin Xia
Summary: Based on previous research, sinomenine has been found to protect mucosal immunity in farmed fish species by preventing intestinal pathological changes and regulating gene expression related to inflammation. It also enhances immune homeostasis and controls the growth of pathogenic bacteria.
