Review
Immunology
Ryley D. McClelland, Tyce N. Culp, David J. Marchant
Summary: Viruses use host factors to enter cells and cause disease, and imaging techniques are crucial for studying virus entry and replication processes, providing insights into viral infection mechanisms. Quantifying viral images and using cryo-electron microscopy to gather structural information of viruses can help identify novel targets for antiviral therapeutics and vaccines.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Immunology
Raffaella Morini, Matteo Bizzotto, Fabio Perrucci, Fabia Filipello, Michela Matteoli
Summary: The role of microglia in controlling synapse homeostasis is increasingly recognized by the scientific community, with potential therapeutic implications in conditions like schizophrenia and Alzheimer's Disease. Methodological approaches to investigate microglial synaptic engulfment include various in vitro assays and co-cultures of microglia and neurons, requiring further validation in mice brain for representative in vivo results. This review aims to analyze the technical approaches to studying microglia-mediated phagocytosis of neuronal and synaptic substrates in critical developmental time windows.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Sarah M. Yannarell, Eric S. Beaudoin, Hunter S. Talley, Alexi A. Schoenborn, Galya Orr, Christopher R. Anderton, William B. Chrisler, Elizabeth A. Shank
Summary: This study investigated the spatial organization and gene expression relationships for important genes in B. subtilis biofilms, expanding the description of cell populations. The results revealed that only a few subpopulations of cells have specific gene expression, while others exhibit simultaneous expression of multiple genes. These findings provide a framework for further studying and predicting the roles of diverse cellular phenotypes in B. subtilis biofilms.
Article
Biotechnology & Applied Microbiology
Hiroyuki Kubota, Masaki Serata, Hoshitaka Matsumoto, Kan Shida, Takekazu Okumura
Summary: This study demonstrates for the first time that CF efflux requires uncompromised glycolytic activity in certain lactic acid bacteria. CF-efflux activity enables the accurate detection of culturable cells, especially in products stored for long periods at cold temperatures. These findings suggest that CF-efflux activity could be an appropriate indicator of cell viability in certain probiotic strains.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Istvan Csomos, Peter Nagy, Csenge Filep, Istvan Rebenku, Eniko Nizsaloczki, Tamas Kovacs, Gyoergy Vamosi, Laszlo Matyus, Andrea Bodnar
Summary: STAT3 is a crucial transcription factor involved in cancer development, with its activation playing a key role. Interleukin-6 is a major activator of STAT3, while chelidonine exerts regulatory effects on STAT3 signaling in treating uveal melanoma cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nanoscience & Nanotechnology
Svetlana Yefimova, Anatolii Onishchenko, Vladimir Klochkov, Valeriy Myasoedov, Yurii Kot, Liliya Tryfonyuk, Oleksandr Knigavko, Pavel Maksimchuk, Umut Kokbas, Yuliia Kalashnyk-Vakulenko, Andrii Arkatov, Vladyslav Khanzhyn, Volodymyr Prokopyuk, Iryna Vyshnytska, Anton Tkachenko
Summary: Rare-earth orthovanadate nanoparticles (ReVO4:Eu3+, Re = Gd, Y or La) have potential as agents for photodynamic therapy of cancer due to their modifiable redox properties. However, their toxicity limits their application. This research aimed to study the pro-eryptotic effects of GdVO4:Eu3+ and LaVO4:Eu3+ nanoparticles and identify the mechanisms of eryptosis induction.
Article
Engineering, Biomedical
Fei Ye, Bei Zhang, Lige Qiu, Yunrui Zhang, Yang Zhang, Jian Zhang, Qingliang Zhao, Ligong Lu, Zhenlin Zhang
Summary: The synergy imaging modality combining capillary microscopy and laser speckle microcirculation imaging allows for dynamic visualization of thrombus events and quantification of hemodynamics information. This method demonstrated excellent thrombolysis effect in an animal model, showing potential for early detection and treatment of thrombus-related diseases in living systems.
MATERIALS TODAY BIO
(2022)
Review
Cell Biology
Nirosha Ganesan, Steven Ronsmans, Peter Hoet
Summary: The ability to monitor lymphocyte responses is crucial for understanding the immune response in humans. The current method, using [H-3] thymidine incorporation, has limitations in accurately measuring cell proliferation. There are several alternative techniques, most notably flow cytometry, that have shown promising results in measuring lymphocyte proliferation. This review evaluates these alternatives and their compatibility with the [H-3] thymidine method to identify the best replacement.
Article
Biochemistry & Molecular Biology
Mariele Montanari, Sabrina Burattini, Caterina Ciacci, Patrizia Ambrogini, Silvia Carloni, Walter Balduini, Daniele Lopez, Giovanna Panza, Stefano Papa, Barbara Canonico
Summary: This study compared the automated-mechanical and enzymatic disaggregation methods for obtaining cell suspensions from various rat tissues. The results showed that these methods worked similarly in some tissues, but the enzyme-free Medimachine II protocol preserved lysosome and mitochondria labeling better, while the enzymatic gentle dissociation consistently induced a lower amount of intracellular ROS.
Article
Optics
Jiarui Li, Hongyuan Zhang, Minjian Lu, Haoyun Wei, Yan Li
Summary: Impulsive stimulated Brillouin spectroscopy (ISBS) is a critical method for fast measurement of mechanical properties. Traditional data processing methods are inefficient in dealing with low signal-to-noise ratio (SNR) signals. In this study, an adaptive noise-suppression Matrix Pencil method is proposed to improve the sensitivity and speed of multi-component viscoelastic identification.
Article
Chemistry, Analytical
Diwakar M. Awate, Cicero C. Pola, Erica Shumaker, Carmen L. Gomes, Jaime J. Juarez
Summary: Despite the limitations of flow cytometers, a 3D-printed imaging platform (3DPIP) has been developed to accurately count particles and conduct fluorescence microscopy efficiently. The 3DPIP utilizes a custom particle counter code and machine vision-based algorithm to identify and count particles, showing comparable accuracy to commercial particle counters. Additionally, the platform can perform static experiments and flow-through experiments effectively, making it a promising tool for use in resource-limited clinical environments.
Article
Hematology
Fatemeh Shahneh, Hans Christian Probst, Sabine C. Wiesmann, Noelia A-Gonzalez, Wolfram Ruf, Kerstin Steinbrink, Verena K. Raker, Christian Becker
Summary: This study investigated the role of inflammatory Ly6C(hi) monocytes in deep vein thrombosis, and found that thrombus formation, growth, and resolution can be therapeutically manipulated with a NR4A1 agonist.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Developmental Biology
Rene Reitermaier, Tanya Ayub, Julia Staller, Philip Kienzl, Nikolaus Fortelny, Pablo Augusto Vieyra-Garcia, Christof Worda, Christian Fiala, Clement Staud, Wolfgang Eppel, Anke Scharrer, Thomas Krausgruber, Adelheid Elbe-Buerger
Summary: This study describes a new workflow for isolating and expanding significant amounts of T cells from fetal human skin. The characteristics of T cells in fetal skin were determined using multiparametric flow cytometry and immunofluorescence techniques. The molecular state of these cells was characterized using single-cell transcriptomics and TCR repertoire profiling.
Article
Pharmacology & Pharmacy
Norival Alves Santos-Filho, Laura Marise de Freitas, Claudia Tavares Dos Santos, Julia Pinto Piccoli, Carla Raquel Fontana, Ana Marisa Fusco-Almeida, Eduardo Maffud Cilli
Summary: The peptide derived from the C-terminal region of the myotoxin BthTX-I showed different mechanisms of action against Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria, indicating its potential as a prototype for new antimicrobial drugs.
Article
Biotechnology & Applied Microbiology
Patricia Lopez-Chicon, Tatiana Riba-Tietz, Oscar Farinas, Pablo-Eduardo Gelber, Ricardo-Pedro Casaroli-Marano, Anna Vilarrodona
Summary: The aim of this study was to compare the effectiveness of fluorescence confocal microscopy and flow cytometry in evaluating the viability of chondrocytes in osteochondral allografts, and to ensure the reproducibility and robustness of the analysis.
BIOENGINEERING-BASEL
(2022)
Review
Biochemistry & Molecular Biology
Jana Patton-Vogt, Anton I. P. M. de Kroon
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2020)
Article
Hematology
Frederik Denorme, Kimberly Martinod, Aline Vandenbulcke, Cecile V. Denis, Peter J. Lenting, Hans Deckmyn, Karen Vanhoorelbeke, Simon F. De Meyer
Summary: The study reveals that VWF plays a role in recruiting proinflammatory monocytes, neutrophils, and T cells to the ischemic brain through its A1 domain. Targeting this domain can reduce immune cell recruitment and protect mice from brain damage caused by ischemic stroke.
Article
Biochemistry & Molecular Biology
Grzegorz Pawlik, Mike F. Renne, Matthijs A. Kol, Anton I. P. M. de Kroon
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Hematology
Elien Roose, An-Sofie Tellier, Edwige Tellier, Gyorgy Sinkovits, Berangere S. Joly, Charlotte Dekimpe, Gilles Kaplanski, Maelle Le Besnerais, Ilaria Mancini, Tanja Falter, Charis Von Auer, Hendrik B. Feys, Marienn Reti, Heidi Rossmann, Aline Vandenbulcke, Inge Pareyn, Jan Voorberg, Andreas Greinacher, Ygal Benhamou, Hans Deckmyn, Rob Fijnheer, Zoltan Prohaszka, Flora Peyvandi, Bernhard Lammle, Paul Coppo, Simon F. De Meyer, Agnes Veyradier, Karen Vanhoorelbeke
Article
Biology
Adela Constantinescu-Bercu, Luigi Grassi, Mattia Frontini, Isabelle I. Salles-Crawley, Kevin Woollard, James T. B. Crawley
Article
Hematology
Sirima Kraisin, Kimberly Martinod, Linda Desender, Inge Pareyn, Sebastien Verhenne, Hans Deckmyn, Karen Vanhoorelbeke, Philippe E. Van den Steen, Simon F. De Meyer
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2020)
Editorial Material
Hematology
Adela Constantinescu-Bercu, Isabelle I. Salles-Crawley, James T. B. Crawley
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2020)
Editorial Material
Hematology
Isabelle I. Salles-Crawley
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Hematology
M. Edward Quach, Wenchun Chen, Yingchun Wang, Hans Deckmyn, Francois Lanza, Bernhard Nieswandt, Renhao Li
Summary: The study identified a novel rat monoclonal antibody 3G6 that acts as a positive modulator of GPIb beta, enhancing GPIb-IX binding and activation. In contrast, another antibody RAM.1 inhibits GPIb-IX signaling, suggesting that changes in GPIb beta conformation may be crucial for outside-in activation via GPIb-IX.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Biochemistry & Molecular Biology
Xue Bao, Martijn C. Koorengevel, Marian J. A. Groot Koerkamp, Amir Homavar, Amrah Weijn, Stefan Crielaard, Mike F. Renne, Joseph H. Lorent, Willie Jc Geerts, Michal A. Surma, Muriel Mari, Frank C. P. Holstege, Christian Klose, Anton I. P. M. de Kroon
Summary: Phosphatidylcholine (PC) is an essential membrane lipid component in most eukaryotes, but its necessity can be suppressed in yeast through cellular evolvability mechanisms involving chromosomal monosomy or a point mutation in the ACC1 gene. These adaptations lead to rewiring of lipid biosynthesis pathways and decreased activity of Acc1, resulting in altered acyl chain length and regulation of membrane fluidity.
Article
Hematology
Adela Constantinescu-Bercu, Yuxiao A. Wang, Kevin J. Woollard, Pierre Mangin, Karen Vanhoorelbeke, James T. B. Crawley, Isabelle I. Salles-Crawleyl
Summary: In this study, a novel GPIbα transgenic mouse (GpIbα(Δsig/Δsig)) was generated using CRISPR-Cas9 technology, and it was found that the intracellular tail of GPIbα plays an important role in transducing VWF-GPIbα and collagen-GPVI signaling events in platelets.
Article
Multidisciplinary Sciences
Yu Huang, Boram Gu, Isabelle I. Salles-Crawley, Kirk A. Taylor, Li Yu, Jie Ren, Xuhan Liu, Michael Emerson, Colin Longstaff, Alun D. Hughes, Simon A. Thom, Xiao Yun Xu, Rongjun Chen
Summary: A biomimetic multiarm nanovesicle designed through bioinspired methods can efficiently deliver tPA and target thrombolysis at the site of thrombus with high selectivity and efficacy. Additionally, a novel computational model is capable of simulating thrombolysis process and has potential application in predicting thrombolysis dynamics in physiological scenarios.
Review
Biochemistry & Molecular Biology
Laura J. Mereweather, Adela Constantinescu-Bercu, James T. B. Crawley, Isabelle I. Salles-Crawley
Summary: Platelets and neutrophils interact with each other, and their interaction is crucial for both arterial and venous thrombosis as well as COVID-19. Targeting platelets and coagulation is effective in treating thrombosis, but it is also accompanied by a bleeding risk. Novel approaches to reduce platelet-mediated recruitment of neutrophils might offer promising therapies for thrombosis without affecting hemostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Hematology
Amna Malik, Anwar A. Sayed, Panpan Han, Michelle M. H. Tan, Eleanor Watt, Adela Constantinescu-Bercu, Alexander T. H. Cocker, Ahmad Khoder, Rocel C. Saputil, Emma Thorley, Ariam Teklemichael, Yunchuan Ding, Alice C. J. Hart, Haiyu Zhang, Wayne A. Mitchell, Nesrina Imami, James T. B. Crawley, Isabelle I. Salles-Crawely, James B. Bussel, James L. Zehnder, Stuart Adams, Bing M. Zhang, Nichola Cooper
Summary: This study investigates the role of cytotoxic CD8(+) T cells in ITP and finds that adults with chronic ITP have increased polyfunctional TEMRA cells expressing interferon gamma, tumor necrosis factor a, and granzyme B. TCR sequencing shows expanded T-cell clones in patients with ITP, which persist over many years and are more prominent in refractory cases. In vitro experiments demonstrate that CD8(+) T cells from patients with ITP form aggregates with autologous platelets, release interferon gamma, and trigger platelet activation and apoptosis via TCR-mediated release of cytotoxic granules.
Review
Hematology
Susan R. Kahn, Donald M. Arnold, Caterina Casari, Karl C. Desch, Katrien M. J. Devreese, Emmanuel J. Favaloro, Florian Gaertner, Samantha C. Gouw, Paolo Gresele, Arjan W. Griffioen, Lukas Heger, R. Manjunatha Kini, Shrey Kohli, Avi Leader, Ton Lisman, Marie Lordkipanidze, Eric Mullins, Helen Chioma Okoye, Rachel P. Rosovsky, Isabelle I. Salles-Crawley, Rita Selby, Michelle Sholzberg, David Stegner, Francesco Violi, Angela C. Weyand, Suzan Williams, Ze Zheng
Summary: The Congress of the International Society of Thrombosis and Haemostasis (ISTH) was held in Montreal, Canada from June 24-28, 2023. The conference showcased cutting-edge advances in various scientific fields related to thrombosis and haemostasis, with a special focus on immuno-thrombosis. The congress covered a wide range of topics including arterial thromboembolism, coagulation and natural anticoagulants, diagnostics and omics, and women's health.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Peripheral Vascular Disease
Jingting Yao, Justin D. Sprick, Jinhee Jeong, Jeanie Park, David A. Reiter
Summary: This study used time-frequency spectral analysis of resting near-infrared spectroscopy (NIRS) signals to evaluate the regulation of peripheral microvascular perfusion in CKD patients. The findings suggest that CKD patients have impaired neurogenic and endothelial functions, which may be associated with increased sympathetic activity and reduced endothelial function in CKD.
MICROVASCULAR RESEARCH
(2024)
Article
Peripheral Vascular Disease
Yoh Sugawara, Yusuke Mizuno, Shinya Oku, Yuri Sawada, Takahisa Goto
Summary: This study investigated the role of Protein kinase D (PKD) in aortic contraction and hemodynamics, finding that PKD1 plays a crucial role in these processes through phosphorylation of MYPT1 and actin polymerization in a calcium-independent manner.
MICROVASCULAR RESEARCH
(2024)
Article
Peripheral Vascular Disease
Xuenan Zhuang, Guiqin He, Yunkao Zeng, Miaoling Li, Yongyue Su, Xuelin Chen, Yining Zhang, Yuhong Gan, Feng Wen, Xiongze Zhang
Summary: This study assessed the impact of acute, heavy alcohol consumption on the ocular microvasculature. The findings suggest that excessive alcohol consumption leads to vasoconstriction in ocular arterioles and vasodilation in capillaries, particularly evident 1-hour post-consumption.
MICROVASCULAR RESEARCH
(2024)
Article
Peripheral Vascular Disease
Ayuki Nakano, Akane Morita, Shiho Arima, Tohru Nagamitsu, Tsutomu Nakahara
Summary: This study examines the preventative and therapeutic effects of inhibiting mammalian target of rapamycin complex 1 (mTORC1) on abnormal retinal blood vessels in a rat model of retinopathy of prematurity (ROP). The results suggest that mTORC1 activation in proliferating endothelial cells contributes to the appearance and progression of ROP-like retinal blood vessels. Inhibition of mTORC1 may be a promising approach for selectively targeting abnormal retinal blood vessels in ROP.
MICROVASCULAR RESEARCH
(2024)
Article
Peripheral Vascular Disease
Bing Wang, Xiaohong Song, Xu Zhang, Yuan Li, Mengting Xu, Xueting Liu, Bingwei Li, Sunjing Fu, Hao Ling, Yingyu Wang, Xiaoyan Zhang, Ailing Li, Mingming Liu
Summary: This study found that glycine supplementation can improve pancreatic microcirculation in T1DM mice and reverse the production of inflammatory cytokines. Furthermore, glycine supplementation also alleviates damage to pancreatic islet and vascular tissues, suggesting a potential therapeutic effect for T1DM.
MICROVASCULAR RESEARCH
(2024)
Article
Peripheral Vascular Disease
Merve Erkisa Genel, Kaan Adacan, Selin Selvi, Deniz Erol Kutucu, Ayca Uvez, Elif Ilkay Armutak, Abdurrahman Sengul, Engin Ulukaya, Ebru Gurel Gurevin
Summary: This study investigated the anti-growth/cytotoxic effect of a dinuclear Pd(II) complex on breast cancer cell lines and confirmed its anti-migration and antiangiogenic properties. The results suggest the potential of this complex in the treatment of breast cancer.
MICROVASCULAR RESEARCH
(2024)
Article
Peripheral Vascular Disease
Cedric Mueller, Christoph Hauser, Justin Carrard, Konstantin Gugleta, Timo Hinrichs, Arno Schmidt-Trucksass, Henner Hanssen, Lukas Streese
Summary: This study aimed to evaluate the differences in microvascular health between normotensive individuals and patients with arterial hypertension and to assess the effects of short-term high-intensity interval training (HIIT) on microvascular health in patients with arterial hypertension. The study found that short-term HIIT can ameliorate hypertension-induced retinal microvascular abnormalities in patients with arterial hypertension.
MICROVASCULAR RESEARCH
(2024)
Article
Peripheral Vascular Disease
Yuyang Hou, Wenjing Du, Qiong Wu, Xinyu Chai, Yinan Wang, Yingqian Mi, Yuan Tian, Mengyan Tang, Jun Li, Dongmei Yan
Summary: This study identified a prognostic signature consisting of four genes in bladder cancer patients and utilized angiogenesis-related genes and molecular subtypes to construct this signature, which could help predict survival outcomes. The study also evaluated immune cell infiltration and predicted chemotherapeutic responses, confirming the effectiveness of this prognostic signature.
MICROVASCULAR RESEARCH
(2024)
Article
Peripheral Vascular Disease
Sean D. Mcgarry, Cynthia Adjekukor, Suchit Ahuja, Jasper Greysson-Wong, Idy Vien, Kristina D. Rinker, Sarah J. Childs
Summary: The article introduces a software tool called Vessel Metrics for analyzing developmental vascular microscopy images, which can expedite the analysis process and ensure consistency between research groups. The tool includes a segmentation algorithm that accurately quantifies different image types and has been validated in zebrafish and mouse models.
MICROVASCULAR RESEARCH
(2024)
Article
Peripheral Vascular Disease
Y. P. Latul, C. Ince, N. E. van Trommel, A. van den Brandhof-van den Berg, J. P. W. R. Roovers, A. W. Kastelein
Summary: This study evaluated the real-time detection capability of handheld vital microscopy (HVM) for cervical intraepithelial neoplasia (CIN) and cervical cancer (CC). The results showed that HVM enables easy, real-time, and non-invasive assessment of cervical lesions through the detection of microvascular alterations. This method has the potential to be used for point-of-care screening, reducing unnecessary surgical interventions.
MICROVASCULAR RESEARCH
(2024)
Article
Peripheral Vascular Disease
Zhifei Liu, Jieqiong Zhang, Xue Li, Qiumei Hu, Xi Chen, Linlin Luo, Liqianyu Ai, Jian Ye
Summary: The study revealed that astrocytic YAP can alleviate retinal pathological angiogenesis by inhibiting overactivation of astrocytes, thereby suppressing excessive VEGF-A production and neuroinflammation.
MICROVASCULAR RESEARCH
(2024)
Article
Peripheral Vascular Disease
Robert G. Hahn
Summary: The fast-exchange interstitial fluid compartment is directly connected to the circulating blood, while the slow-exchange compartment is not.
MICROVASCULAR RESEARCH
(2024)
Review
Peripheral Vascular Disease
Nadia Hamjane, Mohcine Bennani Mechita, Naima Ghailani Nourouti, Amina Barakat
Summary: Gut microbiota dysbiosis plays a crucial role in the development of obesity-related metabolic abnormalities, including insulin resistance, type 2 diabetes, and cardiovascular diseases. Inflammation is a major mechanism linking gut microbiota dysbiosis to obesity-associated metabolic complications.
MICROVASCULAR RESEARCH
(2024)
Article
Peripheral Vascular Disease
Guangzhen Zhou, Yan Gao, Yanbin Shi, Shengnan Qiu, Guimei Lin, Xinbing Ding, Wenguang Wang, Yihua Feng, Fei Wang, Jinwei Qiao
Summary: This study utilized an in vitro biomimetic experimental system to investigate the transvascular transport of nano-preparations, and found that the pressure gradient between vascular fluid pressure and interstitial fluid pressure determines the existence of the enhanced penetration and retention effect of nanopreparations.
MICROVASCULAR RESEARCH
(2024)
Article
Peripheral Vascular Disease
Kenji Chamoto, Barry C. Gibney, Willi L. Wagner, Maximilian Ackermann, Hassan A. Khalil, Steven J. Mentzer
Summary: In the study, the process of angiogenesis in adult regenerating tissue was investigated by grafting portions of the regenerating lung onto the chick chorioallantoic membrane. The results showed peri-graft angiogenesis, particularly in the cardiac lobe. Functional vascular connections were observed between the cardiac lobe and the chick chorioallantoic membrane vascular network. Elevated gene expression levels were found in the cardiac lobe compared to other lobes.
MICROVASCULAR RESEARCH
(2024)