期刊
MICROSCOPY RESEARCH AND TECHNIQUE
卷 75, 期 5, 页码 691-697出版社
WILEY
DOI: 10.1002/jemt.21113
关键词
confocal microscopy; particle tracking; endosomes; lysosomes; polyethylenimine
资金
- National Science Foundation [BES 9978160, BES 0346716]
- National Institutes of Health [T32-GM07057, RC2GM092599]
- ARCS
Using live-cell confocal microscopy and particle tracking technology, the simultaneous transport of intracellular vesicles of the endo-lysosomal pathway and nonviral polyethylenimine (PEI)/DNA nanocomplexes was investigated. Due to potential problems associated with the use of acid-sensitive probes in combination with a gene vector that is hypothesized to buffer the pH of intracellular vesicles, the biological location of PEI/DNA gene vectors was revealed by probing their trafficking in cells expressing fluorescent versions of either early endosome antigen 1, a protein that localizes to early endosomes, or Niemann Pick C1, a protein that localizes to late endosomes and lysosomes. Studies directly show that PEI/DNA nanoparticles are actively transported within both early and late endosomes, and display similar overall transport rates in each. Additionally, gene vector transfer between endosomes is observed. Over time post-transfection, gene vectors accumulate in late endosomes/lysosomes; however, real-time escape of vectors from membrane-bound vesicles is not observed. Microsc. Res. Tech., 2012. (c) 2011 Wiley Periodicals, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据