期刊
MICROSCOPY RESEARCH AND TECHNIQUE
卷 75, 期 4, 页码 492-498出版社
WILEY
DOI: 10.1002/jemt.21082
关键词
CARS tomography; non-linear microscopy; skin imaging; multiphoton imaging
资金
- German Federal Ministry of Education and Research [13N10507]
We present combined epi-coherent anti-Stokes Raman scattering (CARS) and multiphoton imaging with both chemical discrimination and subcellular resolution on human skin invivo. The combination of both image modalities enables label-free imaging of the autofluorescence of endogenous fluorophores by two-photon excited fluorescence, as well as imaging of the distribution of intercellular lipids, topically applied substances and water by CARS. As an example for medical imaging, we investigated healthy and psoriasis-affected human skin with both image modalities in vivo and found indications for different lipid distributions on the cellular level. Microsc. Res. Tech., 2011. (C) 2011 Wiley-Liss, Inc.
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