Article
Anatomy & Morphology
Heitor Hussni Casarin, Vicente Silva Mattos, Jarbas Caiado de Castro Neto, Michelle Alexandra Chinelatti
Summary: The study evaluated the chemical and morphological changes of femtosecond laser-irradiated enamel using Raman spectroscopy, CLSM, and SEM. Results showed a significant decrease in carbonate content and carbonate/phosphate ratio in enamel after laser irradiation, without altering its structure and morphology.
MICROSCOPY RESEARCH AND TECHNIQUE
(2021)
Article
Optics
Kye-Sung Lee, Naresh Kumar Ravichandran, Woo-Jong Yeo, Hwan Hur, Sangwon Hyun, Ji Yong Bae, Dong Uk Kim, Jong Kim, Ki-Hwan Nam, Min Gab Bog, Ki Soo Chang, Geon Hee Kim
Summary: We propose a method to integrate optical coherence tomography (OCT) and spectrally encoded confocal microscopy (SECM) for complementary imaging by adding orthogonal scanning to the SECM configuration. The co-registration of SECM and OCT is automatic, and the system is compact and cost-effective. Speckle noise reduction is achieved by averaging the speckles generated by shifting the spectral-encoded field in the direction of dispersion. The interfaced multimodal imaging of SECM and OCT was implemented at a speed of approximately 7 frames/s using fast-switching technology and GPU processing.
Article
Plant Sciences
Keith E. Duncan, Kirk J. Czymmek, Ni Jiang, August C. Thies, Christopher N. Topp
Summary: This study presents technical advances in using lab-based X-ray microscopy for high-resolution 3D imaging of plant samples at multiple scales. Serial imaging and improved sample preparation methods allow for the generation of sub-micron 3D volumes co-registered with lower magnification scans, providing explicit contextual reference. This method bridges the imaging gap between light and electron microscopy and can be applied to various economically and scientifically important plant systems.
Article
Optics
Danielis Rutkauskas, Egidijus Auksorius
Summary: Full-field optical coherence tomography (FF-OCT) is a camera-based interferometric microscopy technique that can image deep in tissue with high spatial resolution. In this study, digital confocal line scanning is implemented in time-domain FF-OCT using a rolling-shutter camera and digital micromirror device (DMD). The improvement in signal-to-noise ratio by an order of magnitude is demonstrated on a sample.
Article
Dentistry, Oral Surgery & Medicine
Yuan Zhou, Khairul Matin, Yasushi Shimada, Guoqing Wang, Alireza Sadr, Junji Tagami
Summary: The study aims to evaluate early degradation at the resin-dentin interface using non-invasive techniques. Three types of degradation at the interface were identified using SS-OCT. The presence of Bis-GMA affected the gap scale and dentin wall lesion depth in restorative materials.
JOURNAL OF DENTISTRY
(2021)
Article
Biochemical Research Methods
Weikai Xue, Jonas Ogien, Pavel Bulkin, Anne-Lise Coutrot, Arnaud Dubois
Summary: This study aimed to develop a more compact and lighter LC-OCT device that can acquire images faster without significant degradation of resolution. The device is based on a Mirau interferometer using a single objective and incorporates a galvanometer scanner for lateral scanning of the illumination line. The optimized device can acquire B-scans at an unprecedented rate of 17 fps and produce 3D images of human skin at cellular resolution.
JOURNAL OF BIOMEDICAL OPTICS
(2022)
Review
Medicine, Research & Experimental
Arash Dadkhah, Shuliang Jiao
Summary: Photoacoustic microscopy is a hybrid optical imaging technology that provides high-resolution anatomical and functional imaging of biological tissues by imaging optical absorption contrasts with low acoustic scattering. When combined with other imaging modalities, it can reveal complementary information of tissues. Sharing the ultrasonic transducer or light source in a multimodal photoacoustic microscopy system can achieve intrinsically and precisely registered images. The recently developed penta-modal photoacoustic microscopy system features a dynamic focusing technique enabled by OCT contour scan.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Dentistry, Oral Surgery & Medicine
T. C. Pereira, R. J. B. Dijkstra, X. Petridis, P. K. Sharma, W. J. van de Meer, L. W. M. van der Sluis, F. B. de Andrade
Summary: The study found that ultrasonic activation and high flow rate significantly increased the removal of biofilm from the artificial lateral canal, while no significant differences were found between the groups for biofilm removal from the artificial isthmus. RISA and NaOCl exhibited the greatest anti-biofilm efficacy in decontaminating infected dentinal tubules.
INTERNATIONAL ENDODONTIC JOURNAL
(2021)
Article
Optics
Reddikumar Maddipatla, Patrice Tankam
Summary: Combining optical coherence microscopy with fluorescence microscopy allows for the alignment of structural and functional information, enabling new investigations into biological processes in animal models.
OPTICS AND LASERS IN ENGINEERING
(2022)
Article
Optics
Yusi Miao, Mahsa Siadati, Jun Song, Da Ma, Yifan Jian, Mirza Faisal Beg, Marinko Sarunic, Myeong Jin Ju
Summary: The article presents a coherent buffer averaging technique for megahertz-rate retinal imaging to improve OCTA contrast, demonstrating a 10-fold increase in phase alignment among buffers. This method provides better OCTA contrast than conventional approaches with a faster acquisition time.
Article
Dermatology
Silvana Ciardo, Claudia Pezzini, Stefania Guida, Ester Del Duca, Jonathan Ungar, Emma Guttman-Yassky, Marco Manfredini, Francesca Farnetani, Caterina Longo, Giovanni Pellacani
Summary: This study proposes a standardized methodology and defined parameters for objective skin assessments in the cosmetically sensitive cheekbone area of the face, utilizing OCT and RCM features. Through a rigorous validation process, excellent inter-rater reliability was achieved, indicating high reproducibility for semi-quantitative and qualitative parameters. The results may serve as a starting point for standardized application of RCM and OCT in dermo-cosmetic research and practice.
EXPERIMENTAL DERMATOLOGY
(2021)
Article
Anatomy & Morphology
Raquel Viana Rodrigues, Camila Sobral Sampaio, Aline Carvalho Girotto, Caroline Paiuta Pinhatti, Alexsandra Shizue Iwamoto, Anderson Zanardi de Freitas, Glaucia Maria Bovi Ambrosano, Regina Maria Puppin-Rontani, Fernanda Miori Pascon
Summary: This study evaluated the behavior of enamel/restoration interface using FD-OCT, SEM examination, and fluoride release of different restorative materials after a cariogenic challenge. RMGIC showed the highest fluoride release rate, followed by GIC, while RC did not release any fluoride. There were no changes on the restorations margins, but enamel detachment was observed for RC and GIC after pH-cycling.
MICROSCOPY RESEARCH AND TECHNIQUE
(2021)
Article
Food Science & Technology
Liliana Perez-Lavalle, Antonio Valero, Manuel Cejudo-Gomez, Elena Carrasco
Summary: Fresh strawberries are popular and consumed frequently, but outbreaks caused by food-borne pathogens have been associated with their consumption. Salmonella enterica subsp. enterica serovar Thompson can form biofilms on the surface of strawberries during storage, which allows the bacteria to survive and spread. This is crucial for food safety.
Article
Optics
Liheng Shi, Yue Wang, Ruixue Zhang, Jinxu Zhang, Yuetang Yang, Yang Li, Jiayang Chen, Siyu Zhou, Guanhao Wu
Summary: Coherence scanning interferometer (CSI) enables 3D imaging with nanoscale precision. However, the efficiency of such a system is limited because of the restriction imposed by the acquisition system. Here, we propose a phase compensation method that reduces the interferometric fringe period of femtosecond-laser-based CSI, resulting in larger sampling intervals. Experimental results show that our method can maintain a root-mean-square axial error of below 2 nm at a high scanning speed of 6.44 μm per frame, enabling fast nanoscale profilometry over a wide area.
Article
Engineering, Mechanical
M. Gaier, H-T Lin, Z. N. Farhat, K. P. Plucknett
Summary: This study demonstrates the feasibility of using a heat treatable binder phase for TiC-based cermets, showing a significant increase in hardness and scratch resistance with heat treatment. The research provides insights into the effects of heat treatments on stainless steels in metal ceramic composites.
Review
Cell & Tissue Engineering
Gilest. S. Kirby, Andrew Michelmore, Louise E. Smith, Jason D. Whittle, Robert D. Short
Meeting Abstract
Cell & Tissue Engineering
M. T. Elsemary, M. F. Maritz, L. E. Smith, B. Thierry
Meeting Abstract
Cell & Tissue Engineering
P. Ahangar, S. Mills, A. Cowin, L. E. Smith, A. Ting, B. Vaes
Article
Biochemical Research Methods
Scott McCormick, Louise E. Smith, Amy M. Holmes, Ziqiu Tong, Enzo Lombi, Nicolas H. Voelcker, Craig Priest
Article
Cell & Tissue Engineering
Parinaz Ahangar, Stuart J. Mills, Louise E. Smith, Xanthe L. Strudwick, Anthony E. Ting, Bart Vaes, Allison J. Cowin
STEM CELL RESEARCH & THERAPY
(2020)
Article
Cell & Tissue Engineering
Parinaz Ahangar, Stuart J. Mills, Louise E. Smith, Stan Gronthos, Allison J. Cowin
NPJ REGENERATIVE MEDICINE
(2020)
Article
Cell Biology
Parinaz Ahangar, Stuart J. Mills, Louise E. Smith, Xanthe L. Strudwick, Anthony E. Ting, Bart Vaes, Allison J. Cowin
Summary: MAPC cells have shown promising effects on burn injury repair by inhibiting T cell proliferation, reducing inflammation, and promoting angiogenesis. When injected into burn wounds, MAPC cells can decrease wound area, width, and increase re-epithelialization, as well as dampen inflammation and improve angiogenesis in the burn area. However, extracellular matrix production was not significantly affected by MAPC cell treatment. These findings suggest that MAPC cells could be a potential candidate for clinical cell therapies in burn injury repair.
WOUND REPAIR AND REGENERATION
(2021)
Article
Materials Science, Biomaterials
Panthihage Ruvini L. Dabare, Akash Bachhuka, Rahul M. Visalakshan, Hanieh S. Shirazi, Kostya Ostriko, Louise E. Smith, Krasimir Vasilev
Summary: The study showed that surfaces with larger nanoscale topography lead to faster closure of gaps between cell monolayers, mainly driven by cell migration independent of cell proliferation. Surface nanotopography significantly modulates the signaling pathways and proteins involved in cell migration by directly recruiting receptor and adapter proteins responsible for cell-surface interaction.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Article
Cell & Tissue Engineering
Bridget Milky, Michael Zabolocki, Sameer A. Al-Bataineh, Mark van den Hurk, Zarina Greenberg, Lucy Turner, Paris Mazzachi, Amber Williams, Imanthi Illeperuma, Robert Adams, Brett W. Stringer, Rebecca Ormsby, Santosh Poonnoose, Louise E. Smith, Marta Krasowska, Jason D. Whittle, Antonio Simula, Cedric Bardy
Summary: Advances in cellular reprogramming have improved the use of patient-derived cells for neurological research. However, the adherence of human neurons on tissue cultureware is unreliable, hindering their electrophysiological maturation. Thin-film plasma polymer treatments and extracellular matrix coatings can enhance the adherence of human neuronal cultures on glass and improve their electrophysiological properties.
Article
Materials Science, Biomaterials
Mona T. Elsemary, Michelle F. Maritz, Louise E. Smith, Majid Warkiani, Veronika Bandara, Silvana Napoli, Simon C. Barry, Justin T. Coombs, Benjamin Thierry
Summary: CAR-T cell therapy manufacturing process is complex and costly with potential defects. Inertial microfluidics can effectively increase cell viability, reduce cryoprotectant usage, and maintain CAR-T cell functionality.
Meeting Abstract
Oncology
Mona T. Elsemary, Michelle F. Maritz, Louise Smith, Majid Warkiani, Benjamin Thierry
CANCER IMMUNOLOGY RESEARCH
(2021)
Meeting Abstract
Oncology
Mona T. Elsemary, Michelle F. Maritz, Louise Smith, Majid Warkiani, Benjamin Thierry
CANCER IMMUNOLOGY RESEARCH
(2021)
Article
Biophysics
Giles T. S. Kirby, Stuart J. Mills, Tanja E. Mueller, Anthony E. Ting, Allison J. Cowin, Andrew Michelmore, Louise Smith
Meeting Abstract
Cell Biology
P. Ahanger, S. Mills, L. Smith, A. Ting, B. Vaes, A. Cowin
WOUND REPAIR AND REGENERATION
(2020)
Article
Dermatology
T. Fernandez, X. L. Strudwick, S. Al-Bataineh, R. D. Short, A. J. Cowin, J. D. Whittle, L. E. Smith
WOUND PRACTICE AND RESEARCH
(2018)
