期刊
MICROSCOPY AND MICROANALYSIS
卷 19, 期 1, 页码 150-170出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S1431927612014067
关键词
high content; GPCR; adrenergic; parathyroid; arrestin; colocalization; kinetics; image processing; microscopy
资金
- National Institutes of Health [R01 DK55524]
- South Carolina Clinical & Translational Research (SCTR) Institute through NIH/NCRR [TL1 RR029881, TL1 TR000061]
- Foundation Fighting Blindness WG-TRAP [TA-NP-0446-MUSC-WG]
- Department of Education [P200A040143]
- NIH [T32 GM08716, C06 RR-015455]
We report the development of a method to analyze receptor and beta-arrestin2 mobilization between Class A and B GPCRs via time-resolved fluorescent microscopy coupled with semiautomated high-content multiparametric analysis. Using transiently expressed, tagged beta 2-adrenergic receptor (beta(2)-AR) or parathyroid hormone receptor type 1 (PTH1R), we quantified trafficking of the receptors along with the mobilization and colocalization of coexpressed tagged beta-arrestin2. This classification system allows for exclusion of cells with nonoptimal characteristics and calculation of multiple morphological and spatial parameters including receptor endosome formation, beta-arrestin mobilization, colocalization, areas, and shape. Stimulated Class A and B receptors demonstrate dramatically different patterns with regard to beta-arrestin interactions. The method provides high kinetic resolution measurement of receptor translocation, which allows for the identification of the fleeting beta-arrestin interaction found with beta 2-AR agonist stimulation, in contrast to stronger mobilization and receptor colocalization with agonist stimulation of the PTH1R. Though especially appropriate for receptor kinetic studies, this method is generalizable to any dual fluorescence probe system in which quantification of object formation and movement is desired. These methodologies allow for quantitative, unbiased measurement of microscopy data and are further enhanced by providing real-time kinetics.
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