Article
Multidisciplinary Sciences
A. E. Herguedas-Alonso, L. Aballe, J. Fullerton, M. Velez, J. I. Martin, A. Sorrentino, E. Pereiro, S. Ferrer, C. Quiros, A. Hierro-Rodriguez
Summary: The use of magnetic vector tomography/laminography allows access to nanoscale magnetization through a 3D experimental window. However, the large number of angular projections required for these methods leads to long measurement times. In this study, a fast method is presented to significantly reduce experiment time for quasi two-dimensional magnetic systems. The algorithm utilizes the Beer-Lambert equation in X-ray transmission microscopy to achieve 3D magnetic configuration of the sample. This method has been successfully demonstrated in permalloy microstructures and provides quantitative results with a reduced number of angular projections. The throughput of this methodology is x 10-x 100 times faster than conventional magnetic vector tomography, making it of great interest to the scientific community.
SCIENTIFIC REPORTS
(2023)
Article
Biochemical Research Methods
Jie E. Yang, Matthew R. Larson, Bryan S. Sibert, Joseph Y. Kim, Daniel Parrell, Juan C. Sanchez, Victoria Pappas, Anil Kumar, Kai Cai, Keith Thompson, Elizabeth R. Wright
Summary: In this paper, robust tools for montage parallel array cryo-tomography (MPACT) tailored for vitrified specimens are presented, which can image large fields of view while preserving high-resolution structural information. The combination of multiple techniques in MPACT supports studies that contextually define the three-dimensional architecture of cells, and it provides flexibility and efficient data processing and analysis.
Article
Spectroscopy
Haiyang Xian, Yiping Yang, Shan Li, Jianxi Zhu, Rui Li, Jieqi Xing, Jiaxin Xi, Hongmei Yang, Xiaoju Lin, Xiao Wu, Hongping He
Summary: Electron tomography (ET) is an effective technique used in structural biology and physical sciences to provide 3D structural information with atomic-scale resolution. This study verifies the applicability of ET in earth and planetary science research and demonstrates its capability to observe the 3D morphology of mineral grains and chemical components distribution in earth and planetary materials.
ATOMIC SPECTROSCOPY
(2022)
Article
Chemistry, Physical
Yoon Jang Chung, K. A. Villegas Rosales, K. W. Baldwin, P. T. Madathil, K. W. West, M. Shayegan, L. N. Pfeiffer
Summary: By improving sample quality and innovating materials and vacuum chamber design, researchers have achieved a breakthrough in GaAs quantum well systems with two-dimensional electrons, demonstrating high mobility and low residual impurities.
Article
Materials Science, Multidisciplinary
Zhongquan Jiang, Danqing Sun, Huize Guan, Yutong Sun, Menglei Ye, Lin Zhang, Tingting Gu, Jiani Chen, Shujie Wang, Chunhua Zhang, Shimei Wang, Zhenlei Zhou, Ying Ge, Zhen Li
Summary: The ULLS technique offers a novel approach for preparing ultra-thin sections for TEM analysis, simplifying the sample preparation process and providing acceptable toxicity levels for most microbial cells. It holds promise for playing a significant role in studying the ultrastructure of microbial cells.
MICROSCOPY AND MICROANALYSIS
(2021)
Article
Cell Biology
Roger Olofsson Bagge, Jens Berndtsson, Ornella Urzi, Jan Lotvall, Massimo Micaroni, Rossella Crescitelli
Summary: This study presents the first 3D visualization of extracellular vesicles (EVs) in human tissue using transmission electron tomography. The spherical structures of EVs were observed in the interstitium of both metastatic and normal liver tissues.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Multidisciplinary Sciences
Semih Barutcu, Selin Aslan, Aggelos K. Katsaggelos, Doga Gursoy
Summary: Computed tomography is widely used for reconstructing the three-dimensional structure of objects, but faces challenges in limited angle reconstruction. To address this, researchers have utilized generative models and deep networks to effectively constrain the solution space.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Robert Kiewisz, Gunar Fabig, William Conway, Daniel Baum, Daniel Needleman, Thomas Mueller-Reichert
Summary: In this study, the three-dimensional structure of kinetochore fibers in mammalian cells was characterized using large-scale electron tomography. It was found that each kinetochore fiber is composed of approximately nine kinetochore microtubules, with variations in circumference and density along their length. The association between kinetochore microtubules and non-kinetochore microtubules was primarily observed in the spindle pole regions.
Article
Multidisciplinary Sciences
Marian Boktor, Benjamin R. Ecclestone, Vlad Pekar, Deepak Dinakaran, John R. Mackey, Paul Fieguth, Parsin Haji Reza
Summary: Histopathological visualizations play a crucial role in modern medicine and biological research. This paper presents a deep-learning-based approach for virtual label-free histochemical staining of unstained tissue using total-absorption photoacoustic remote sensing (TA-PARS) images. The method has been validated against traditional staining techniques and confirmed to produce high-quality images suitable for diagnostic interpretation. This approach paves the way for slide-free histological imaging, which could significantly reduce the time required for histopathology.
SCIENTIFIC REPORTS
(2022)
Review
Cell Biology
Snjezana Radulovic, Sowmya Sunkara, Reinhard Rachel, Gerd Leitinger
Summary: A major aim in structural cell biology is to analyze intact cells in three dimensions, visualize subcellular structures, and even localize proteins at the best possible resolution in three dimensions. Recently developed electron microscopy tools offer great resolution in three dimensions, but the challenge is to investigate smaller volumes. Different approaches, including array tomography, batch tomography, and scanning transmission electron tomography, were presented at the Microscopy Conference in Vienna in 2021 to overcome this challenge and provide increased 3D information.
HISTOCHEMISTRY AND CELL BIOLOGY
(2022)
Article
Electrochemistry
Moritz Kroll, Sarah L. Karstens, Marvin Cronau, Alexandra Hoeltzel, Sabine Schlabach, Nikita Nobel, Claudia Redenbach, Bernhard Roling, Ulrich Tallarek
Summary: By utilizing focused ion-beam scanning electron microscopy tomography, this study presents a three-phase reconstruction model of a lithium-ion battery cathode containing the carbon binder domain (CBD), representing the pore space morphology effectively. Results reveal the CBD as a convoluted network that dominates the pore space structure and limits Li+ transport through tortuous and constricted diffusion pathways.
BATTERIES & SUPERCAPS
(2021)
Article
Biology
Sebastian Stroeh, Eric W. Hammerschmith, David W. Tank, H. Sebastian Seung, Adrian Andreas Wanner
Summary: The in situ time-lapsed X-ray-assisted staining procedure allows real-time observation of individual staining steps in electron microscopy and measurement of heavy metal accumulation in large tissue samples immersed in different staining solutions. The accumulation of osmium in fixed tissue was found to have a quadratic dependence on incubation time and sample size. This method provides insights for optimizing staining protocols for large samples.
Article
Biology
Ryuji Yanase, Flavia Moreira-Leite, Edward Rea, Lauren Wilburn, Jovana Sadlova, Barbora Vojtkova, Katerina Pruzinova, Atsushi Taniguchi, Shigenori Nonaka, Petr Volf, Jack D. Sunter
Summary: Attachment to a substrate is a common strategy among eukaryotic parasites for maintaining position in a specific ecological niche. In this study, the researchers used advanced electron microscopy techniques to investigate the adhesion of Leishmania parasites to the stomodeal valve in the sand fly vector. They found distinct structural elements in the attachment plaque and identified five stages of haptomonad-like cell differentiation. The study also demonstrated the importance of calcium for Leishmania adhesion in vitro. These findings provide important insights into the structural and regulatory aspects of Leishmania adhesion and contribute to a better understanding of its life cycle.
Article
Chemistry, Medicinal
Sandhya P. Tiwari, Florence Tama, Osamu Miyashita
Summary: XFEL scattering technology faces challenges in studying single biomolecular complexes, leading to the proposal of an alternative method to find potential 3D biological shape models for further analysis.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)
Article
Multidisciplinary Sciences
Haonan Zhang, Yan Li, Yanan Liu, Dongyu Li, Lin Wang, Kai Song, Keyan Bao, Ping Zhu
Summary: Researchers developed a deep learning-based method called REST for denoising and restoring information in cryo-electron tomography. The method performed well in both simulated and real datasets and was able to reveal the dynamic states of target molecules.
NATURE COMMUNICATIONS
(2023)
Correction
Endocrinology & Metabolism
Ryuta Osumi, Ziyi Wang, Yoshihito Ishihara, Naoya Odagaki, Tadahiro Iimura, Hiroshi Kamioka
JOURNAL OF BONE AND MINERAL METABOLISM
(2021)
Article
Endocrinology & Metabolism
Ryuta Osumi, Ziyi Wang, Yoshihito Ishihara, Naoya Odagaki, Tadahiro Iimura, Hiroshi Kamioka
Summary: The study revealed that mechanical unloading significantly decreased bone density in the distal metaphysis of the femur, causing Scl distribution to be closer to the marrow on the anterior and medial side, while increasing Scl expression only on the lateral side. Transmission electron microscopy findings showed that only the expression of Scl in canaliculi was affected by mechanical unloading.
JOURNAL OF BONE AND MINERAL METABOLISM
(2021)
Article
Dentistry, Oral Surgery & Medicine
Jaeyeon Lim, Chihiro Tanikawa, Mikihiko Kogo, Takashi Yamashiro
Summary: This study used artificial intelligence systems to determine prognostic factors for orthognathic surgery in children with cleft lip and/or palate. Factors identified included the number of clefts, palatal repair method, male sex, cephalometric variables, growth patterns, and number of missing teeth.
ORTHODONTICS & CRANIOFACIAL RESEARCH
(2021)
Article
Dentistry, Oral Surgery & Medicine
Chihiro Tanikawa, Ayaka Oka, Jaeyoen Lim, Chonho Lee, Takashi Yamashiro
Summary: The study estimated the number of cephalograms needed to re-learn for different quality images when artificial intelligence systems are introduced in a clinic by testing different data sets and comparing the success rates.
ORTHODONTICS & CRANIOFACIAL RESEARCH
(2021)
Article
Dentistry, Oral Surgery & Medicine
Koji Fujiyama, Yohei Kera, Shimizu Yujin, Chihiro Tanikawa, Takashi Yamashiro, Xiaohan Guo, Andy Ni, Toru Deguchi
Summary: The study shows that both Invisalign and conventional fixed appliances are effective in treating patients with a severe deep overbite. Invisalign therapy may be more preferable for patients with high angle and deep overbite compared to conventional fixed appliance therapy.
AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Yuujin Shimizu, Chihiro Tanikawa, Tomoyuki Kajiwara, Hajime Nagahara, Takashi Yamashiro
Summary: This study aimed to evaluate the performance of two artificial intelligence systems in creating prioritized problem lists and treatment plans, and compare them with orthodontists. The results showed that the AI systems performed similarly to orthodontists in some aspects, but not as well in others.
EUROPEAN JOURNAL OF ORTHODONTICS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Jun Maeda, Chihiro Tanikawa, Namiki Nagata, Jaeyeon Lim, Sven Kreiborg, Shumei Murakami, Takashi Yamashiro
Summary: The study evaluated the errors of three-dimensional mandibular surfaces generated using MRI data compared to the gold standard of CT. Random errors were quantified for the 3-D rendering of the mandible, with most regions showing minimal detectable change values that allowed for evaluation. The coronoid region had the greatest errors, but overall there was 'almost perfect' agreement between the CT and MRI imaging.
ORTHODONTICS & CRANIOFACIAL RESEARCH
(2022)
Review
Endocrinology & Metabolism
Takashi Yamashiro, Hiroshi Kurosaka, Toshihiro Inubush
Summary: The Runx family genes are crucial transcriptional regulators in tissue development. Recent studies have revealed their novel roles in tooth, salivary gland, and palate development. Future studies may uncover additional roles of Runx signaling pathways in morphogenesis.
CURRENT OSTEOPOROSIS REPORTS
(2022)
Article
Anatomy & Morphology
Sakhr Ahmed Murshid
Summary: Lacunar-canalicular (LC) permeability and fluid flow-shear stress/drag force play important roles in mechanotransduction in bone tissue by inducing mechanical stimuli in osteocytes, modulating cellular functions, and determining bone adaptation. Alterations in LC structure may affect the ability of osteocytes to sense and translate mechanical signals, potentially contributing to bone remodelling. This review discusses recent studies on LC networks, their formation and transfer of mechanical stimuli, and changes in structure, functional permeability, and mechanotransduction resulting from age, pathology, and mechanical loading. Applications of vibration and low-intensity pulsed ultrasound in bone healthcare and regeneration fields are also presented.
Article
Genetics & Heredity
Toshihiro Inubushi, Yuichiro Nakanishi, Makoto Abeid, Yoshifumi Takahata, Riko Nishimura, Hiroshi Kurosaka, Fumitoshi Irie, Takashi Yamashiro, Yu Yamaguchi
Summary: HA degradation mediated by TMEM2 plays a crucial role in neural crest development and the morphogenesis of neural crest derivatives.
Article
Dentistry, Oral Surgery & Medicine
Sayuri Yamamoto, Chihiro Tanikawa, Takashi Yamashiro
Summary: The morphology of the human face varies widely due to genetic and environmental factors. This study focused on the 3D facial morphology of Japanese adults with skeletal Class 1 malocclusion and examined the association between sexual dimorphism and shape variations.
AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Yuka Murata, Chihiro Tanikawa, Yuki Shiraishi, Toshihiro Inubushi, Kana Kono, Hiroshi Kamioka, Takashi Yamashiro
Summary: Masticatory activity affects the skeletal development of the cranial base, particularly leading to morphological changes in the sphenoid bone.
EUROPEAN JOURNAL OF ORTHODONTICS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Yuka Murata, Hiroshi Kurosaka, Takumi Hirose, Makoto Fujiwara, Yoko Miyoshi, Masayo Kagami, Kaori Hara-Isono, Keiichi Ozono, Takashi Yamashiro
Summary: This article describes comprehensive orthodontic treatment for an 11-year-old girl with SRS, achieving improvement in facial profile and occlusion through two phases of treatment. Long-term follow-up is also necessary.
CLINICAL AND INVESTIGATIVE ORTHODONTICS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Chihiro Tanikawa, Yuko Shintaku, Hiroshi Yoshikawa, Takashi Yamashiro
Summary: This study developed a novel method for accurately superimposing an intraoral digital model on CBCT images based on palatal mucosa coated with barium sulphate. It was found that the errors when using CBCT images obtained with barium sulphate coating were significantly smaller than when using uncoated images.
CLINICAL AND INVESTIGATIVE ORTHODONTICS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Hirotaka Ueda, Naoki Oka, Tsuyoshi Shimo, Akira Sasaki, Takashi Yamashiro, Hiroshi Kamioka
Summary: This article presents the orthognathic treatment of an adult patient with skeletal Class III malocclusion, facial asymmetry, and anterior disc displacement without reduction (ADDwoR) at the right temporomandibular joint (TMJ) using intraoral vertical ramus osteotomy (IVRO). The IVRO procedure successfully corrected the mandibular deviation and improved the symptoms of temporomandibular disorders. The results demonstrate the potential effectiveness of IVRO in addressing facial asymmetry and anterior disc displacement in patients with mandibular deviation.
CLINICAL AND INVESTIGATIVE ORTHODONTICS
(2022)