期刊
MICROGRAVITY SCIENCE AND TECHNOLOGY
卷 21, 期 4, 页码 311-318出版社
SPRINGER
DOI: 10.1007/s12217-008-9092-6
关键词
Magnetic levitation; MC3T3; Osteoblastic cell; Microgravity
资金
- NIH [1R21EB003947, 1S10RR16783-01]
- Super-computing Institute for Advanced Computational Research of the University of Minnesota
Diamagnetic samples placed in a strong magnetic field and a magnetic field gradient experience a magnetic force. Stable magnetic levitation occurs when the magnetic force exactly counter balances the gravitational force. Under this condition, a diamagnetic sample is in a simulated microgravity environment. The purpose of this study is to explore if MC3T3-E1 osteoblastic cells can be grown in magnetically simulated hypo-g and hyper-g environments and determine if gene expression is differentially expressed under these conditions. The murine calvarial osteoblastic cell line, MC3T3-E1, grown on Cytodex-3 beads, were subjected to a net gravitational force of 0, 1 and 2 g in a 17 T superconducting magnet for 2 days. Microarray analysis of these cells indicated that gravitational stress leads to up and down regulation of hundreds of genes. The methodology of sustaining long-term magnetic levitation of biological systems are discussed.
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