期刊
MICROCHIMICA ACTA
卷 174, 期 3-4, 页码 247-255出版社
SPRINGER WIEN
DOI: 10.1007/s00604-011-0623-2
关键词
Copper(II) ions; Fluorescent probe; Fluorescence enhancement; Rhodamine derivatives; Fluorescence imaging; Living cells
资金
- National Natural Science Foundation of China [20975089]
- Department of Science and Technology of Shandong Province of China [2008GG20005005]
- Chinese Academy of Sciences [KZCX2-EW-206]
- Shandong Province Doctor Fund [2008BS04031]
A fluorescent probe for Cu(II) ion is presented. It is based on the rhodamine fluorophore and exhibits high selectivity and sensitivity for Cu(II) ion in aqueous methanol (2:8, v/v) at pH 7.0. The response is based on a ring opening reaction and formation of a strongly fluorescent 1:1 complex. The response is reversible and linear in the range between 50 nM and 900 nM, with a detection limit of 7.0 nM. The probe was successfully applied to fluorescent imaging of Cu(II) ions in HeLa cells.
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